Effects of pair housing on behavior, cortisol, and clinical outcomes during quarantine-like procedures for rhesus macaques (Macaca mulatta).

BACKGROUND: Compatible pair housing of macaques in research settings increases species-typical behaviors and facilitates beneficial social buffering. It is not yet established whether these benefits are maintained after intrafacility transfer and domestic quarantine, which are two stressors that can lead to behavioral and clinical abnormalities. METHODS: We evaluated 40 adolescent male rhesus macaques who were single- or pair-housed immediately following an intrafacility transfer. We measured behavior, fecal cortisol, body weight, and diarrhea occurrence. Body weight and diarrhea occurrence were also retrospectively analyzed in an additional 120 adolescent rhesus who underwent a similar transfer. RESULTS AND CONCLUSIONS: Pair-housed macaques exhibited less of some undesirable behaviors (e.g., self-clasping) and experienced less diarrhea than single-housed subjects; however, no significant differences in cortisol levels or alopecia measures were found. The demonstrated beneficial effects of pair housing for rhesus macaques following intrafacility transfer and adjustment suggest pairing upon arrival at a new facility will bolster animal welfare.

Harmonisation of welfare indicators for macaques and marmosets used or bred for research.

Background: Accurate assessment of the welfare of non-human primates (NHPs) used and bred for scientific purposes is essential for effective implementation of obligations to optimise their well-being, for validation of refinement techniques and novel welfare indicators, and for ensuring the highest quality data is obtained from these animals. Despite the importance of welfare assessment in NHP research, there is little consensus on what should be measured. Greater harmonisation of welfare indicators between facilities would enable greater collaboration and data sharing to address welfare-related questions in the management and use of NHPs. Methods: A Delphi consultation was used to survey attendees of the 2019 NC3Rs Primate Welfare Meeting (73 respondents) to build consensus on which welfare indicators for macaques and marmosets are reliable, valid, and practicable, and how these can be measured. Results: Self-harm behaviour, social enrichment, cage dimensions, body weight, a health monitoring programme, appetite, staff training, and positive reinforcement training were considered valid, reliable, and practicable indicators for macaques (≥70% consensus) within a hypothetical scenario context involving 500 animals. Indicators ranked important for assessing marmoset welfare were body weight, NHP induced and environmentally induced injuries, cage furniture, huddled posture, mortality, blood in excreta, and physical enrichment. Participants working with macaques in infectious disease and breeding identified a greater range of indicators as valid and reliable than did those working in neuroscience and toxicology, where animal-based indicators were considered the most important. The findings for macaques were compared with a previous Delphi consultation, and the expert-defined consensus from the two surveys used to develop a prototype protocol for assessing macaque welfare in research settings. Conclusions: Together the Delphi results and proto-protocol enable those working with research NHPs to more effectively assess the welfare of the animals in their care and to collaborate to advance refinement of NHP management and use.

Predictors of Long-Term Social Compatibility in Rhesus Macaques (Macaca mulatta) Housed in Research Settings.

Social housing improves the well-being of monkeys in research settings; however, little is known about factors influencing the long-term stability of established, full-contact pairs. Archival data were examined to determine whether sex, age, weight, duration pair housed, familiarity, social interruptions, room changes, or sedation events predicted eventual separation of pairs for social incompatibility (n = 80) or for nonsocial reasons (e.g., research or health needs) (n = 1143). Using a logistic regression model (Wald Χ2(8) = 42.325, p < .001), three significant factors were identified. Pairs in which partners had known prior familiarity in group housing were less likely to experience social incompatibility (p = .034). Pairs housed together longer (p < .001) and who staff had temporarily separated through the placement of a cage divider to reduce physical contact were more likely to require permanent separation for social incompatibility (p < .001); additional analysis revealed that dividers were often placed for social reasons, suggesting early signs of social instability. Findings may be useful for primate caregivers when making decisions about managing social partners.

The identification of effective welfare indicators for laboratory-housed macaques using a Delphi consultation process.

Despite the importance for both animal welfare and scientific integrity of effective welfare assessment in non-human primates, there has been little or no consensus as what should be assessed. A Delphi consultation process was undertaken to identify the animal- and environment-based measures of welfare for laboratory-housed macaques and to determine their relative importance in on-site welfare assessments. One-hundred fifteen potential indictors were identified through a comprehensive literature search, followed by a two-round iterative electronic survey process to collect expert opinion. Stable group response and consensus about the validity, reliability, and feasibility of the proposed indicators (67.5% agreement) was achieved by the completion of Round Two. A substantially higher proportion of environment-based measures (72%: n = 44/61) were considered as valid, reliable, and feasible compared to the animal-based measures (22%: n = 12/54). The indicators that ranked most highly for assessing welfare were the presence of self-harm behaviours and the provision of social enrichment. This study provides an empirical basis upon which these indicators can be validated and then integrated into assessment tools developed for macaques and emphasises the need to include both animal- and environment-based indicators for accurate welfare monitoring.

Two Methods of Social Separation for Paired Adolescent Male Rhesus Macaques (Macaca mulatta).

Literature-based recommendations regarding how to separate pairs of laboratory-housed NHP when required for research, veterinary, or management needs are unavailable. This study assessed 2 separation techniques-rapid and stepwise-to determine whether a period of limited social access mitigates the behavioral stress response after complete separation. Researchers observed 12 pairs of mother-reared, adolescent male rhesus macaques before and after separation with a solid divider; 6 of the pairs experienced a transitional week of limited social access through a perforated panel. Observers collected 30-min focal animal scans during study phase, totaling 144 h of behavioral data. Target behaviors included those classified as protest, agitation, tension, and withdrawn or self-directed. Social separation resulted in a significant change in behavior. Separated monkeys displayed more withdrawn or self-directed behaviors (for example, huddling, self-directed stereotypies) than when pair-housed. Stepwise separation resulted in increased agitation behaviors in the limited contact phase and did not mitigate separation effects. Adverse behavioral changes during the limited contact phase support continuous pair housing until required individual housing.

SSI/SSDI Outreach, Access, and Recovery (SOAR): Disability Application Outcomes Among Homeless Adults.

OBJECTIVE: The SSI/SSDI Outreach, Access, and Recovery (SOAR) program has been shown to increase access to Supplemental Security Income and Social Security Disability Insurance benefits among homeless adults. However, little empirical data exist on how or for whom SOAR achieves successful application outcomes. This study investigated applicant and application characteristics associated with disability application outcomes among homeless adults. METHODS: Secondary data on 6,361 SOAR-assisted applications were obtained. Multilevel models investigated between-applicant differences in application processing time and decision as a function of applicant and application characteristics. RESULTS: Older age and living in an institution were associated with greater odds of application approval. Female gender and receipt of public assistance were associated with longer processing time and lower odds of approval. Except for quality review, SOAR critical components were associated with greater odds of approval. CONCLUSIONS: Women and adults receiving public assistance appear disadvantaged in the SOAR application process. SOAR critical components promote successful disability application outcomes.

Pair housing of Macaques: A review of partner selection, introduction techniques, monitoring for compatibility, and methods for long-term maintenance of pairs.

Pair housing of macaques has become a widely implemented compromise between meeting the social needs of the monkeys and allowing for their use in biomedical research. While beneficial to the animals, pair housing can provide challenges for those caring for them. Drawing from both scientific literature and direct experience, this paper provides a review of practical aspects of pair housing including partner selection, pairing methodologies, staff education, and equipment considerations. Recommendations include selecting a pairing method appropriate to the facility and the individual animals being paired, educating staff on social behavior, and establishing a pair monitoring program to facilitate long-term pair maintenance. Assessment of behavior is essential in determining the compatibility of new pairs and in identifying established pairs that may need interventions to enhance their long-term compatibility. The pair housing program at the Yerkes National Primate Research Center is described as one model of a successful program. Am. J. Primatol. 79:e22485, 2017. © 2015 Wiley Periodicals, Inc.