RESUMO
During their first participation in the external quality assessment program (EQAP), 78-81% of the laboratories detect positive samples of control panels containing 0.125 IU/ml of HBsAg; 95-97% of them identify negative samples of the control panels. The high efficiency of EQAP was established when the results of the same laboratories that had participated in two cycles were compared. For correct detection of positive samples of the control panel, it is necessary to use disposable containers and tips for example for solutions of conjugate concentrate, tetramethylbenzidine, buffer for conjugate dilution, and citrate-phosphate solution. The ranges of 95% confidence interval were established for the individual values of CV values of the commercial preparation ILC-HBsAg in the laboratories: from 0 to 11.3% with estimated convergence and from 0 to 14.2% with estimated reproducibility. In making an intralaboratory control of the quality of HBsAg test using ILC-HBsAg, one should be guided by the test accuracy indices given in the application sheet.
Assuntos
Técnicas de Laboratório Clínico , Antígenos de Hepatite/sangue , Hepatite/sangue , Técnicas de Laboratório Clínico/normas , Hepatite/diagnóstico , Humanos , Programas Nacionais de Saúde , Garantia da Qualidade dos Cuidados de Saúde , Reprodutibilidade dos TestesRESUMO
Slight cooling had no effect on heat emission and heat production, but modulated the immune response to antigen in animals. Changes in the immune response upon rapid slight cooling of the skin (by 1.5 degrees C) depended on the phase of immunogenesis corresponding to cold exposure. When cooling was performed immediately after immunization, antibody production increased in the spleen and blood, while antigen binding in the spleen remained unchanged. Cold exposure on day 5 after antigen treatment as well as immunization at the peak of cooling did not modulate antibody production, but increased antigen binding in the spleen. Our findings attest to an important role of the temperature factor in the formation of the immune response, which should be taken into account during vaccination.