RESUMO
The present study investigated the prevalence of blood chloride (Cl) abnormalities in diarrheic neonatal calves with metabolic acidosis and attempted to identify the most relevant electrolyte abnormality to these abnormalities. A retrospective analysis was conducted on the medical records of 157 diarrheic neonatal calves aged 10.3 ± 4.2 days old with metabolic acidosis. Hypochloremia, normochloremia, and hyperchloremia were observed in 8.9% (14/157), 43.3% (68/157), and 47.8% (68/157), respectively, of diarrheic calves with metabolic acidosis. This distribution remained similar regardless of age (under 8 days or 8 days and older). Furthermore, a multiple logistic regression analysis showed that variations in values for blood sodium [Na (regression coefficients 0.877; 95% confidence interval (CI) 13.977-134.195; P<0.01)], pH (regression coefficients -10.719; 95% CI -19.076- -2.362; P<0.05), and bicarbonate [HCO3- (regression coefficients -0.555; 95% CI -0.820- -0.290; P<0.01)] were associated with blood Cl abnormalities. The present results revealed that blood Na concentrations were more strongly associated with blood Cl concentrations than blood pH and HCO3- values. In the present study, diarrheic calves with hyperchloremia were characterized by normonatremia and extremely severe metabolic acidosis.
Assuntos
Acidose , Animais Recém-Nascidos , Doenças dos Bovinos , Cloretos , Diarreia , Animais , Bovinos , Acidose/veterinária , Acidose/sangue , Doenças dos Bovinos/sangue , Cloretos/sangue , Animais Recém-Nascidos/sangue , Estudos Retrospectivos , Diarreia/veterinária , Diarreia/sangue , Masculino , Feminino , Sódio/sangue , Concentração de Íons de Hidrogênio , Bicarbonatos/sangueRESUMO
The present paper reports on the clinical efficacy and optimal clinical dose of medetomidine for sedation of young cows during dehorning surgery. Medical records of 24 female Holstein cows that underwent dehorning surgery were used in this study. In four groups, the sedation of animals was carried out by one of the four intravenous treatments: 0.1 mg kg-1 xylazine (Xyl group, n = 6), 5.0 µg kg-1 medetomidine (5.0 Med group, n = 6), 10.0 µg kg-1 medetomidine (10.0 Med group, n = 6) or 20.0 µg kg-1 medetomidine (20.0 Med group, n = 6). The clinical sedation score (CSS) and heart rate (HR) were recorded. The CSSs after intravenous administration of each α2-adrenergic receptor agonist increased rapidly and peaked at 12.5 (10.0-16.0) at t = 20 min in the Xyl group, 11.5 (10.0-15.0) at t = 10 min in the 5.0 Med group, 16.0 (14.0-16.0) at t = 20 min in the 10.0 Med group and 16.0 (14.0 - 16.0) at t = 20 min in the 20.0 Med group. A similar degree of bradycardia was observed after every sedative treatment. We conclude that the intravenous administration of 10.0-20.0 µg kg-1 medetomidine is appropriate for sedation of young cows without severe side effects.
Assuntos
Anestesia , Medetomidina , Feminino , Bovinos , Animais , Medetomidina/farmacologia , Imidazóis/farmacologia , Hipnóticos e Sedativos/farmacologia , Anestesia/veterinária , Xilazina/farmacologia , Frequência CardíacaRESUMO
This study aimed to clarify the relationship between acute phase protein (APP) concentrations and serum Fe concentrations to determine whether serum iron (Fe) can be clinically applied as a substitute for APPs in cows. One hundred five Holstein-Friesian breed lactating dairy cows were enrolled in this study. Cows with inflammatory diseases were 16 subclinical, and 15 severe mastitis cows, in addition to 15 mild and 16 severe sole ulcer cows. The plasma haptoglobin (HPT), alpha-1 acid glycoprotein (AGP), SAA, serum Fe levels, and other biochemical parameters in the cows were measured. The two-sample t-tests and multiple logistic regression analysis were used to compare the control and inflammatory disease groups. ROC analysis was used to evaluate the ability to diagnose inflammation disease. From the results, the proposed diagnostic cutoff value for plasma SAA and serum Fe concentrations to identify dairy cows with inflammatory diseases based on analyses of ROC curves were set at > 3.65 mg/l and < 120.50 µg/dl, respectively. Therefore, instead of using expensive inflammatory markers to evaluate the inflammatory state at the first treatment day for inflammatory diseases in cow, it shows the useful for screening with serum Fe concentration that can be measured easily and inexpensively as alternative inflammatory biomarkers.
Assuntos
Doenças dos Bovinos , Lactação , Feminino , Bovinos , Animais , Soro/metabolismo , Proteínas de Fase Aguda/análise , Inflamação/diagnóstico , Inflamação/metabolismo , Biomarcadores/metabolismo , Leite/química , Doenças dos Bovinos/metabolismoRESUMO
The economic efficiency of dairy farms is directly influenced by reproductive performance. Reproductive performance is affected by postpartum health; however, it is unknown which diseases reduce reproductive performance or how often these diseases occur in tie-stall dairy farms. In this study, the 15 most frequent diseases in tie-stalls and their impact on reproduction were determined by logistic analysis. Data from reproductive and veterinary records of 814 cows (473 Holstein) calving between April 2016 and March 2018 reared in 9 tie-stall commercial dairy farms were used. The 15 most frequent diseases were identified by medical records. The odds ratio (OR) of the diseases reducing reproductive performance (days open, number of artificial insemination, and first artificial insemination days in milk) was measured by logistic regression analysis. We focused on mastitis, and factors (inflammation grade, causative bacteria) having a negative effect on reproduction were measured similarly. Of the 15 most frequent diseases, endometritis (OR: 5.45, p < 0.001), ovarian quiescence (OR: 3.95, p < 0.001), mastitis (OR: 2.49, p < 0.001), and follicular cyst (OR: 2.33, p = 0.006) significantly prolonged days open after adjusting for environmental factors (farm, parity, and calving season). Focusing on mastitis, mastitis with mild inflammation significantly prolonged days open. Mastitis and genital diseases were frequent diseases that reduced reproductive performance. Strategies to reduce the incidence of these diseases are considered effective for the economics of tie-stall dairy farms.
RESUMO
The present study aimed to clarify the alkalizing ability of 1.35% isotonic sodium bicarbonate solution (ISBS), which did not contain dextrose, compared with that of 1.35% isotonic bicarbonate sodium solution containing 4.03% dextrose (ISBD) in healthy calves. The calves were intravenously administered with 20.7 mL/kg of the solutions for 30 min as the volume required to correct base deficit of 10 mM. ISBS increased the blood pH, HCO3-, and base excess from 7.44 ± 0.02, 29.6 ± 1.9 mM, and 5.3 ± 2.1 mM to 7.49 ± 0.02, 36.9 ± 2.3 mM, and 13.5 ± 2.6 mM respectively (P<0.05). These factors for the ISBD group increased from 7.41 ± 0.02, 29.0 ± 1.1 mM, and 4.5 ± 1.3 mM to 7.43 ± 0.02, 33.5 ± 1.9 mM, and 9.5 ± 1.7 mM (P<0.05), respectively. Furthermore, in the ISBD group, the relative plasma volume and blood glucose level increased while the K+ level decreased, which did not occur in the ISBS group. Therefore, the results revealed that ISBS had better alkalizing ability in calves than ISBD.
Assuntos
Acidose , Doenças dos Bovinos , Acidose/veterinária , Animais , Bicarbonatos , Glicemia , Bovinos , Soluções Isotônicas , Sódio , Bicarbonato de Sódio/farmacologiaRESUMO
Very few epidemiologic studies have verified the utility of the right paramedian abomasopexy (RPA) technique in cows with right abomasal disorders. This study aimed to investigate the outcomes and risk factors for non-survival in the herd within 30 days of surgery in cows with right abomasal disorders who underwent the RPA technique. Forty-seven Holstein cows with right abomasal disorders (25 with right abomasal displacement [RDA] and 22 with right abomasal volvulus [RAV]) were included. Twenty-two cows with RDA (22/25, 88.0%) and 10 cows with RAV (10/22, 45.5%) survived at 30 days post-surgery. Multivariate logistic regression analysis indicated that hyponatremia, hypokalemia, and the presence of abomasal volvulus were the major risk factors associated with non-survival.
Assuntos
Abomaso , Abomaso/cirurgia , Animais , Bovinos , Feminino , Estudos Retrospectivos , Fatores de RiscoRESUMO
The aims of studies were to estimate the withdrawal period of antibiotic from milk after the intramammary infusion of cefazolin sodium (CEZ) in cows with difficulties in frequent milk discharge due to disease such as teat injury. The period was compared among cows milked twice a day after 150 or 450 mg of CEZ were administered to all quarters (Study 1, 2) and the cows in which milking of front-right quarter was ceased for five days after administration of these infusions to only that quarter (Study 3). In Studies 1 and 2, the median of 17.66 µg/ml and 83.18 µg/ml of CEZ were detected in the samples of first milking after intramammary administration, respectively; however, there was no residual antibiotic by 72 hr in all cows. In Study 3, the median of 1.96 µg/ml of CEZ was detected in the sample after the resumption of milking at 120 hr, and the residual was eliminated by 174 hr. The withdrawal period may be prolonged by the cessation of milking after administration, and the period is the total time from cessation to 72 hr after the resumption of milking.
Assuntos
Indústria de Laticínios , Lactação , Animais , Antibacterianos , Bovinos , Cefazolina , Feminino , Glândulas Mamárias Animais , LeiteRESUMO
Point-of-care (POC) devices that veterinary practitioners can use to easily and rapidly measure blood ionized calcium (iCa) levels in cows immediately after withdrawing a blood sample on the dairy farm are needed. Aims of present studies was to compare the commercially available ion-selective electrode handheld iCa meter (bovine blood iCa checker) with the benchtop blood gas analyzer GEM premier 3500 and handheld analyzer i-STAT 1. Sixty-two paired-point whole blood samples were obtained from three cows with hypocalcemia experimentally induced by Na2-EDTA infusion. Whole blood samples were also obtained from the 36 cows kept on a farm in field conditions. The results using the bovine blood iCa checker correlated with those using the GEM premier 3500 and i-STAT 1. Bovine blood iCa checker was "compatible" with the GEM premier 3500 and i-STAT 1 because the frequency of differences between the measurements within ± 20% of the mean were 100% (65/65, >75%) and 90.8% (59/65, >75%), respectively. In the field trial, the blood iCa concentration measured by the bovine blood Ca checker was significantly positively correlated with that measured by the i-STAT 1 portable analyzer. Bovine blood iCa checker was "compatible" with the i-STAT 1 because the frequency of differences between the measurements within ± 20% of the mean was 100% (36/36, >75%). Results from these findings, the bovine blood iCa checker may be applied as a simplified system to measure the iCa concentration in bovine whole blood.
Assuntos
Doenças dos Bovinos , Hipocalcemia , Animais , Gasometria/veterinária , Cálcio , Bovinos , Feminino , Testes Hematológicos/veterinária , Hipocalcemia/diagnóstico , Hipocalcemia/veterináriaRESUMO
We hypothesized that the serum iron (Fe) concentration in cows with respiratory diseases is a satisfactory substitute for major inflammatory markers such as haptoglobin (HPT) and serum amyloid A (SAA). Twenty Japanese Black cows aged 279.6 ± 120.0 days were enrolled, and divided into respiratory diseases and control groups based on the presence of clinical findings of respiratory diseases. As a result, area under the receiver operating characteristic curves for plasma HPT, SAA and serum Fe concentrations for respiratory disease-associated systemic inflammation were excellent, at 1.00, 0.96 and 0.97, respectively. Therefore we confirmed that the serum Fe concentration is a satisfactory substitute for HPT and SAA in beef cows with respiratory diseases.
Assuntos
Doenças dos Bovinos , Ferro , Animais , Biomarcadores , Bovinos , Doenças dos Bovinos/diagnóstico , Feminino , Haptoglobinas , Proteína Amiloide A SéricaRESUMO
This study established the precision and accuracy of a modified latex agglutination turbidimetric immunoassay (LATIA) reagent, and evaluated the ability of the measurement of serum amyloid A (SAA) compared to haptoglobin and α1-acid glycoprotein, which are acute phase proteins (APPs), for diagnosis of clinical mastitis. Concentrations of APPs in cows with mastitis were significantly higher than those in healthy cow. Only the plasma SAA concentration in cows with clinical mastitis (44.90 mg/l; n=15) was significantly higher than that in those with subclinical mastitis (10.70 mg/l; n=16), enabling their diagnosis in contrast to other APPs. Thus, the SAA assay using a LATIA reagent is useful in assessing mastitis severity due to its higher sensitivity and specificity than other APP assays.
Assuntos
Doenças dos Bovinos , Mastite Bovina , Animais , Bovinos , Feminino , Haptoglobinas , Imunoturbidimetria/veterinária , Testes de Fixação do Látex/veterinária , Mastite Bovina/diagnóstico , Leite , Orosomucoide , Proteína Amiloide A SéricaRESUMO
We hypothesize that some amino acid abnormalities in diarrheic calves are useful for understanding intestinal mucosal damage, as in humans. However, few reports have revealed the relationship between intestinal mucosal damage and plasma amino acids in diarrheic calves. Therefore, the aim of present study was to investigate whether there is a relationship between the amino acid status and plasma diamine oxidase (DAO) activity, which is known to be a biomarker for intestinal mucosal damage in diarrheic calves. Twenty Holstein calves aged 12.6 ± 4.2 days old were enrolled in this study. In the diarrhea group (n = 10), there were yellow loose feces within the rectum and Cryptosporidium parvum (C. parvum) was detected in all fecal samples. These calves were clinically normal except for diarrhea. All calves in the control group (n = 10) appeared to be healthy based on clinical findings with normal feces production and the absence of C. parvum. Plasma amino acid concentrations and DAO activity were measured. The relationships between plasma DAO activity and the concentration of each plasma amino acid were investigated using Spearman's rank test. The plasma DAO activity was significantly lower in the diarrhea group (176.1 ± 60.1 IU mL-1) than in the control group (309.3 ± 74.8 IU mL-1) (p < 0.001). Furthermore, positive correlations were observed when comparing plasma DAO activity with histidine, proline, cystine, arginine, and glutamine concentrations. As a result of relationship between plasma DAO activity and amino acid status, it was concluded that plasma amino acid status is useful for understanding intestinal mucosal damage in calves with cryptosporidiosis.
Assuntos
Aminoácidos/sangue , Criptosporidiose/sangue , Mucosa Intestinal/patologia , Amina Oxidase (contendo Cobre)/sangue , Animais , Biomarcadores/sangue , Bovinos , Criptosporidiose/patologia , Cryptosporidium parvum/isolamento & purificação , Diarreia/sangue , Diarreia/parasitologia , Diarreia/patologia , Diarreia/veterinária , Fezes/parasitologiaRESUMO
The aim of this study was to examine whether 2.16% hypertonic saline solution (HSS) is useful for the treatment of diarrheic calves with hyponatremia. Eleven of 13 female Holstein calves exhibiting moderate diarrhea and hyponatremia received 1,250 ml of 2.16% HSS over 15 min regardless of body weight. The remaining two calves that were unable to stand and had severe hyponatremia received 2,500 ml of 2.16% HSS intravenously over 30 min. As a result, hyponatremia in all diarrheic calves was significantly improved by the administration of 2.16% HSS from 122.2 ± 7.0 mEq/l at pre to 134.8 ± 3.7 mEq/l at post, which was above the threshold of 132 mEq/l for hyponatremia. Therefore, 2.16% HSS may be useful for hyponatremia in calves with diarrhea.
Assuntos
Doenças dos Bovinos , Hiponatremia , Animais , Bovinos , Doenças dos Bovinos/tratamento farmacológico , Diarreia/tratamento farmacológico , Diarreia/veterinária , Feminino , Hiponatremia/tratamento farmacológico , Hiponatremia/veterinária , Solução Salina Hipertônica/uso terapêutico , SódioRESUMO
The aim of study was to investigate the relationships among serum diamine oxidase (DAO) activity, postnatal days and the plasma copper (Cu) concentration, using calves with or without diarrhea. In healthy calves, the serum DAO activity was significantly higher at 2 postnatal days than at ≥7 postnatal days, and no significant changes were observed after 7 postnatal days. In addition, no significant correlation was found between serum DAO activity and plasma Cu concentration at all postnatal days in healthy calves. Although, the serum DAO activity in 14 diarrheic calves (66.78 ± 14.37 IU/ml) was lower than that in 19 healthy calves (170.33 ± 97.83 IU/ml, P<0.01), plasma Cu concentrations in all calves remained within the normal range.
Assuntos
Amina Oxidase (contendo Cobre) , Doenças dos Bovinos , Amina Oxidase (contendo Cobre)/metabolismo , Animais , Biomarcadores , Bovinos , Diarreia/veterinária , OxirreduçãoRESUMO
The aim of present study was to evaluate the precision of plasma haptoglobin (HPT), serum iron (Fe) and plasma transferrin (Tf) concentrations as biomarkers of the severity of acute mastitis (AM) in cows. Fourteen Holstein Friesian cows with AM were divided into severe (n=8) and mild groups (n=6) based on systemic and local inflammation, and 12 healthy cows were also enrolled as controls. As a result, significant changes were observed in plasma HPT and serum Fe concentrations. The proposed cut-off points for plasma HPT and serum Fe concentrations for the severity of AM in cows based on receiver operating characteristic analyses were >10.3 µg/ml and <49.0 µg/dl, respectively. No significant difference was observed in the plasma Tf concentration.
Assuntos
Doenças dos Bovinos , Mastite Bovina , Mastite , Animais , Biomarcadores , Bovinos , Doenças dos Bovinos/diagnóstico , Feminino , Haptoglobinas/análise , Inflamação/veterinária , Ferro , Mastite/veterinária , Mastite Bovina/diagnóstico , Leite/químicaRESUMO
Specific alterations in plasma histidine concentrations and diamine oxidase (DAO) activity were recently reported as a potential biomarker for intestinal mucosal damage in diarrheic calves. However, there are no data on the comparison of precision between histidine concentration and DAO activity in bovine plasma. The aim of the present study was to compare precision of histidine concentrations and DAO activities in plasma as a biomarker for the Cryptosporidium parvum (C. parvum)-associated intestinal mucosal damage in diarrheic calves. Thirty-two Holstein calves aged 12.2 ± 4.1 days old were enrolled in the present study; they were divided into C. parvum (n = 9), diarrhea (n = 11), and control (n = 12) groups based on the presence or absence of diarrhea and with or without C. parvum infection. Receiver operating characteristic (ROC) curves were used to characterize the sensitivity and specificity of each parameter for the C. parvum-associated intestinal mucosal damage. The proposed cut-off points for plasma histidine concentrations and plasma DAO activities for cryptosporidiosis in calves based on ROC analyses were < 55.8 nM and < 246.0 IU/ml, respectively. The sensitivities and specificities of the proposed diagnostic cut-offs were 88.9% and 82.6% for plasma histidine concentrations and 100.0% and 34.8% for plasma DAO activities, respectively. It was concluded that plasma histidine concentrations may be superior to plasma DAO activities as a specific biomarker for the C. parvum-associated intestinal mucosal damage in diarrheic calves.
Assuntos
Doenças dos Bovinos/patologia , Criptosporidiose/patologia , Histidina/sangue , Mucosa Intestinal/patologia , Animais , Biomarcadores/sangue , Bovinos , Doenças dos Bovinos/microbiologia , Criptosporidiose/microbiologiaRESUMO
The objective of the present study was to elucidate sequential changes in mRNA abundance of serum amyloid A (SAA) isotypes in endotoxin (ETX) challenge model cattle. Ten healthy cattle were separated to 2 groups: control and ETX groups. Cattle in the ETX group were challenged by 2.5 µg/kg of O111:B4 lipopolysaccharide in 4 ml of autologous serum. Blood samples were withdrawn at pre, 0.5, 1, 2, 4, 8, 12, 24, 48, 72 and 96 hr after ETX challenge. Plasma ETX activity, serum SAA concentrations, mRNA abundance of interleukin (IL)-6, SAA2 and SAA4 in the liver and polymorphonuclear leukocytes were measured. The plasma ETX activity in the ETX group increased at 0.5 hr after the ETX challenge. The serum SAA value remained higher between 12 and 72 hr after the ETX challenge than that of the control group. Hepatic IL-6 mRNA abundance in the ETX group increased at 2 hr after the ETX challenge. Hepatic SAA2 and SAA4 mRNA abundance significantly increased from 4 hr after administration, and remained significantly higher than those pre-values up to 12 and 24 hr, respectively. The abundance ratio of hepatic SAA2 was much higher than that of SAA4. The major isotype was SAA2 in liver tissue, and it is indicating systemic inflammation in cattle.
Assuntos
Inflamação/veterinária , Lipopolissacarídeos/toxicidade , Fígado/metabolismo , Proteína Amiloide A Sérica/análise , Animais , Bovinos , Inflamação/induzido quimicamente , Interleucina-6 , Lipopolissacarídeos/sangue , Neutrófilos/metabolismo , RNA Mensageiro/metabolismo , Proteína Amiloide A Sérica/genética , Proteína Amiloide A Sérica/metabolismoRESUMO
The objective of this study was to examine whether serum iron (Fe) concentration is useful as a prognostic biomarker for cows with acute coliform mastitis (ACM). Our study was composed of determining the reproducibility of serum Fe concentration as a prognostic criterion in cows with ACM (Study 1) and clarifying the sequential changes in serum Fe concentration in cattle that received endotoxin (Study 2). Seventy-seven cows with (n = 47) or without (n = 30) ACM were enrolled in Study 1. The proposed diagnostic cut-off value of serum Fe concentration indicating a poor prognosis of ACM based on the analysis of the receiver operating characteristic curves was < 31.5 µg/dL. Ten young cattle aged 176.8 ± 23.7 days were enrolled in Study 2. Five young cattle received endotoxin (LPS group) and the remaining five received physiological saline (control group). Blood collections were carried out before endotoxin challenge (pre), and 0.5, 1, 2, 4, 8, 12, 24, and 48 h after the challenge. As a result, a significant decrease in serum Fe concentration was not observed until 24 h after endotoxin challenge. Because in cows with clinical ACM it is difficult to know the time course after infection, the alteration in serum Fe concentrations alone may be an insufficient prognostic criterion.
Assuntos
Endotoxemia/veterinária , Infecções por Escherichia coli/veterinária , Ferro/sangue , Infecções por Klebsiella/veterinária , Mastite Bovina/diagnóstico , Doença Aguda , Animais , Biomarcadores/sangue , Bovinos , Endotoxemia/diagnóstico , Endotoxemia/microbiologia , Endotoxinas/administração & dosagem , Escherichia coli/fisiologia , Infecções por Escherichia coli/complicações , Infecções por Escherichia coli/diagnóstico , Infecções por Escherichia coli/microbiologia , Feminino , Infecções por Klebsiella/complicações , Infecções por Klebsiella/diagnóstico , Infecções por Klebsiella/microbiologia , Klebsiella pneumoniae/fisiologia , Mastite Bovina/microbiologia , Prognóstico , Reprodutibilidade dos TestesRESUMO
The aim of the present study was to investigate whether abnormalities in plasma diamine oxidase (DAO) activity reflect the degree of intestinal mucosal disorder in calves with diarrhea. A total of 50 Holstein calves were enrolled. Thirty-six of the 50 calves presented diarrhea and were sub-classified by severity based on fecal status (0: firm, 1: pasty, 2: loose, and 3: watery) and blood pH (acidemia: blood pH <7.25) as follows: Seventeen calves exhibiting watery diarrhea and/or fall into acidemia were sub-categorized into the severe group. The other nineteen calves exhibiting pasty or loose diarrhea and not fall into acidemia were sub-categorized into the moderate group. The remaining 14 calves without diarrhea were assigned to the control group. The plasma DAO activity was significantly lower (pâ¯<â¯.01) in the calves with severe or moderate diarrhea than in the control group. In addition, the plasma DAO activity was significantly lower (pâ¯<â¯.05) in the severe group than in the moderate group. The relationship between plasma DAO activity and fecal score (râ¯=â¯-0.55, pâ¯<â¯.01) in calves with diarrhea were found to have significantly and negatively correlated by Spearman's rank test in this study. Our results suggested that plasma DAO activity reflect the degree of intestinal mucosal disorder due to diarrhea.
Assuntos
Doenças dos Bovinos/sangue , D-Aminoácido Oxidase/sangue , Diarreia/veterinária , Mucosa Intestinal/patologia , Acidose , Animais , Biomarcadores/sangue , Bovinos , Diarreia/sangue , Diarreia/patologia , FezesRESUMO
The aim of this study was to evaluate the accuracy and precision of portable blood glucose meters, such as i-STAT 1 and Precision Xceed, for use in calves. Whole blood and plasma samples were obtained from eleven calves that received 2.5 or 5.0% dextrose-containing polyelectrolyte isotonic solutions. Measurements using the i-STAT 1 (r2=0.99, P<0.0001) and Precision Xceed (r2=0.96, P<0.0001) were well correlated with those by the hexokinase method, which is the gold standard. Although the accuracy of i-STAT 1 was equivalent to that of the hexokinase method, there was an autocorrelation in the residuals between the results from the Precision Xceed and the hexokinase method. Thus, the i-STAT 1 can be used to measure the blood glucose concentration in cattle.
Assuntos
Análise Química do Sangue/veterinária , Glicemia/análise , Bovinos/sangue , Animais , Análise Química do Sangue/instrumentação , Glucose/administração & dosagem , Soluções Isotônicas/administração & dosagem , Sistemas Automatizados de Assistência Junto ao LeitoRESUMO
The aim of this study was to investigate the relationship between the endotoxin activity in plasma and that in bronchoalveolar lavage fluid (BALF) in bronchopneumonia. Thirty-three calves were included in this study (17 healthy calves and 16 calves with respiratory disease). In the calves with bronchopneumonia, the median endotoxin activity in plasma (0.437 EU/ml, P<0.001) and BALF (29.45 EU/ml, P<0.001) was significantly higher than in the control calves. Plasma endotoxin activity was significantly and positively correlated with that in BALF (r2=0.900, P<0.001). Based on the receiver operating characteristics curves, we propose a diagnostic cutoff point for plasma endotoxin activity (0.104 EU/ml, AUC=0.914, P<0.001, Se 81.3% and Sp 82.4%) for identification of bronchopneumonia in calves which could die within a week.