Effect of climate change on current and future potential distribution of Strawberry tree (Arbutus unedo L.) in Türkiye.

The demand for nontimber forest products (NTFPs) has increased significantly in recent years. Hundreds of plant species that grow naturally in Türkiye have medicinal and aromatic value. Medicinal and aromatic plants are primarily used as a sources of tea, spices, condiments and essential oils. Species belonging to the genus Arbutus L. are used for decorative purposes and as fuel wood in many wood-based industries, they also have a wide range of uses in packaging, chairs making and furniture production. Additionally, the fruits of these trees are widely consumed by humans and animals because they are rich in sugar and vitamin C. It is predicted that changes in climatic conditions will significantly change the distribution, composition and function of forests threatening biodiversity. The purpose of this study was to model current and future potential geographical distributions of Arbutus unedo L., which is among the species that naturally grow in Türkiye and is of substantial value in terms of its ecological contribution to forest ecosystems, based on species presence data and environmental variables (bioclimatic variables and altitude). The current and future distribution area models for Arbutus unedo L. indicate that the potential distribution areas of the species in the coming years will gradually decrease, and in the SSP5 8.5 model, which represents the highest level of world resource usage this gradual decrease will reach its highest level and there will be no suitable distribution area left for the species. Therefore, it is predicted that the species will become endangered. In-situ and ex-situ conservation measures need to be taken to ensure the sustainability of the species in forestry and landscape areas.

Novel data on genotoxic assessment of bismuth sulfide nanoflowers in common carp Cyprinus carpio.

The environmental impacts and risks of nanomaterials that are commonly used in different technologies are of great concern as their toxic effects on the aquatic ecosystem remain unclear. In this study, bismuth sulfide (Bi2S3) nanoflowers (nfs) were synthesized using a microwave-based hydrothermal process, and their genotoxic effects were investigated in the common carp, Cyprinus carpio. Bi2S3 nanoflowers were applied to common carp for 96 h. LC50 value (LC50 = 350 mg/L-1) was determined for acute toxicity with probit analysis, and three sublethal concentrations (35, 87, and 175 mg/L-1) were selected accordingly for genotoxicity tests. Such LC50 value - 350 mg L-1 for the common carp makes these nanoflowers non-toxic to aquatic organisms according to the EU-Directive 93/67/EEC classification scheme. Toxicological evaluations of the sublethal concentrations of Bi2S3 nanoflowers demonstrated that the 35 and 87 mg L-1 Bi2S3nfs groups were generally harmless and similar to the control group. Only the 175 mg L-1 Bi2S3nfs group had significant DNA damage frequency and nuclear abnormalities than the control and other Bi2S3nfs groups. To the best of our knowledge, this is a novel data on genotoxicity reported for fish species exposed to Bi2S3 nanoflowers; however, further systematic studies need to be performed to fully estimate the effects of Bi2S3 nanoflowers on aquatic life.

Bioaccumulation of trace metals and genotoxicity responses in Liza aurata as an indicator of industrial pollution.

Heavy metal contamination in the coastal and marine ecosystems is becoming a serious risk to aquatic organisms and humans. This study reports the effects, including genetic damage, of accumulations of trace metals on Liza aurata, which is used as a bio-indicator species, in the Payas coast of Iskenderun Bay, north-eastern Mediterranean by COMET Assay. L. aurata were seasonally collected from a sampling site and a reference site for one year. Physicochemical parameters in water and trace metals in the tissues of fish collected from these sites were determined by electrochemical techniques. High DNA damage frequency in L. aurata was observed along the Payas coast of Iskenderun Bay compared to the reference site because of pollutants. The detected high levels of Cd, Pb, Fe and Cu accumulation in L. aurata exceed the maximum levels allowed by the national and international limit values. Significant positive correlations between Cd, Pb, Hg, Cr, Fe, Zn, and Cu accumulations and DNA damage parameters were observed in the present study. Additionally, we first reported the successful use of the electrochemical technique in the determination of trace metal concentrations in mullet. Moreover, L. aurata constitutes a key tool as a sentinel organism for biomonitoring of coastal ecosystems.

Effect of modulating glutamate signaling on myelinating oligodendrocytes and their development-A study in the zebrafish model.

Myelination is crucial for the development and maintenance of axonal integrity, especially fast axonal action potential conduction. There is increasing evidence that glutamate signaling and release through neuronal activity modulates the myelination process. In this study, we examine the effect of manipulating glutamate signaling on myelination of oligodendrocyte (OL) lineage cells and their development in zebrafish (zf). We use the "intensity-based glutamate-sensing fluorescent reporter" (iGluSnFR) in the zf model (both sexes) to address the hypothesis that glutamate is implicated in regulation of myelinating OLs. Our results show that glial iGluSnFR expression significantly reduces OL lineage cell number and the expression of myelin markers in larvae (zfl) and adult brains. The specific glutamate receptor agonist, L-AP4, rescues this iGluSnFR effect by significantly increasing the expression of the myelin-related genes, plp1b and mbpa, and enhances myelination in L-AP4-injected zfl compared to controls. Furthermore, we demonstrate that degrading glutamate using Glutamat-Pyruvate Transaminase (GPT) or the blockade of glutamate reuptake by L-trans-pyrrolidine-2,4-dicarboxylate (PDC) significantly decreases myelin-related genes and drastically declines myelination in brain ventricle-injected zfl. Moreover, we found that myelin-specific ClaudinK (CldnK) and 36K protein expression is significantly decreased in iGluSnFR-expressing zfl and adult brains compared to controls. Taken together, this study confirms that glutamate signaling is directly required for the preservation of myelinating OLs and for the myelination process itself. These findings further suggest that glutamate signaling may provide novel targets to therapeutically boost remyelination in several demyelinating diseases of the CNS.

Heavy metal bioaccumulation, oxidative stress and genotoxicity in African catfish Clarias gariepinus from Orontes river.

One of the major problems affecting the ecosystem health is the exposure of heavy metals due to anthropogenic activities. These exposures may induce toxiogenetic damage and carcinogenesis in aquatic organism. DNA damage biomarkers for fish species detect genotoxic parameters for ecological risk assessment. In the present study, the effect of heavy metals (Cr, Cd, Cu, Fe and Mn) on DNA damage and oxidative stress of Clarias gariepinus was examined by Comet assay at Reference site and two different sampling sites of the Orontes River. Moreover, the relationship between DNA damage and physiological response to oxidative stress caused in C. gariepinus was investigated by sampling seasonal water and fish samples for one year. In this study, Cr, Cd and Cu in water column of Orontes River and the concentrations of Fe and Mn in liver tissue of C. gariepinus were highly exceed the maximum permissible limit set by EPA (1999, 2016) WHO (1989) and TEG (2012) that can cause adverse effects on environment health. SOD activities in liver tissues were significantly higher than those in muscle tissues. SOD, CAT activity and MDA levels of both Site 1 and Site 2 at Orontes River were higher than those of the Reference site. In result of the COMET analysis, the highest levels of DNA damage were found at gill and liver tissues in Site 2 (17.746 ± 1.072% T-DNA; 16.014 ± 0.710% T-DNA respectively) at Orontes river. A higher level of DNA damage in gill cells compared to liver cells was observed in all regions of the Orontes River. In Principal component analysis (PCA), the heavy metals Cd, Cr and Cu (in the water column) and MDA in liver and muscle revealed strong contribution to the observed DNA damage that were scattered opposite to each other's along the principal components. Moreover, correlations between parameters revealed a positive significant relationship between Cd, Cr and Cu in water and DNA damage levels in C. gariepinus. Pearson correlation analysis detected a positive relationship between MDA, CAT and SOD and DNA damage levels in African catfish. Consequently, this study exposed genotoxic damage and oxidative stress of C. gariepinus due to metal pollution in Orontes River and lead to the better understanding of genotoxicty, oxidative stress and heavy metal relationships.

Effect of heavy metals on toxicogenetic damage of European eels Anguilla anguilla.

Aquatic organisms are exposed to a variety of contaminants such as heavy metals introduced into the environment as a consequence of anthropogenic activities that usually cause genotoxic damage in aquatic organisms. DNA damage biomarkers for fish species detect genotoxic parameters for ecological risk assessment. In the present study, the effect of heavy metals (Cd, Cr, Cu, Fe, Ni, Pb, Zn, Co, and Mn) on DNA damage of Anguilla anguilla was examined by comet assay at reference site and three different sampling sites of the Orontes River. The relative mean heavy metal concentrations in water column were in the order of Fe>Cr>Pb>Zn>Ni>Cu>Mn>Cd>Co in all the sampling sites. Cr, Cd, and Pb concentrations at all sampling sites were above the values allowed by the EPA (1999, 2016). With these results, negative effects of Cr, Cd, and Pb appeared on ecosystem health. The comet assay showed a higher level of DNA damage in the gill cells in comparison with the liver cells of A. anguilla. The highest level of DNA damage as %T-DNA, tail moment, and tail migration in gill cells were 20.007 ± 1.744 %; 2.899 ± 0.341 µm, and 12.383 ± 01.040 TMi and 20.172 ± 1.944 %, 2.559 ± 0.265 µm, and 10.763 ± 0.910 TMi at Site 2 and Site 3, respectively. The correlations between heavy metals and DNA damage parameters revealed that both Cu and Co in water showed significant negative correlations (p < 0.05) with DNA damage levels. Consequently, this study revealed the genotoxic damage of A. anguilla due to pollution in Orontes River and lead to the better understanding of genotoxicity and heavy metal relationships.

The effects of Gokshura, Tribulus terrestris on sex reversal of guppy, Poecilia reticulata.

This study examined the effects of Tribulus terrestris (TT) on sex reversal in guppy, Poecilia reticulata. The objective of this study was to introduce a new environmentally friendly method for masculinization in P. reticulata. Since male guppy has higher commercial value than female. TT is a natural, non-toxic herb which helps enhance testosterone levels in human and animals. It was prepared in a laboratory in France. Different concentration (0.0, 0.05, 0.1 and 0.15 g L(-1)) of TT was investigated for sex reversal in the Poecilia reticulata. TT extract was administered by immersion of newly born offspring once weekly for two months. Among the dosages used in the present study 0.15 g L(-1) TT was the most effective dosage that ensured maximum male ratio (80%, p < 0.01). Although, sex ratios of 0.05 and 0.1 g L(-1) TT were not significantly different from the expected 1:1 ratio, in these two groups treatment with TT also result in higher number of males (58.25 and 59.77%, respectively), than control (p > 0.05). Total survival rates in all treatments and control were uniformly high ranging from 83 to 87% (p > 0.05). It is concluded that TT has no negative effect on survival rate of P. reticulata. All groups of TT-treated fish exhibited successful growth acceleration comparing to the control group, but only TT treatment at the concentration of 0.15 and 0.1 g L(-1) TT significantly improved growth rate of P. reticulata (p < 0.01). Histological examinations revealed that testes of fish treated with TT-extract contained all stages of spermatogenesis. Sex reversal in P. reticulata demonstrated that TT treated new-born progenies showed successful sex reversal, spermatogenesis and better growth rate than untreated progenies.