RESUMO
Scanning electron microscopy study showed that exposure to CO for 60, 75, and 90 min induced heterogeneous changes in erythrocyte population. Increasing the duration of exposure of blood erythrocytes to CO was followed by the appearance of cells with morphological changes. The formation of discocytes with processes (≥1) was followed by the appearance of "deflated ball"-shaped erythrocytes. Moreover, CO modulated activity of glucose-6-phosphate dehydrogenase in human erythrocytes and disturbed their energy metabolism (suppressed lactate dehydrogenase activity in forward reaction and increased it in reverse reaction). A significant decrease in the coefficient of energy metabolism of erythrocytes (from 36±14 to 5.0±2.5 arb. units) reflected metabolic maladaptation induced by the exposure to CO.
Assuntos
Monóxido de Carbono/farmacologia , Metabolismo Energético , Eritrócitos/metabolismo , Adolescente , Adulto , Catálise , Eritrócitos/citologia , Feminino , Glucosefosfato Desidrogenase/metabolismo , Voluntários Saudáveis , Humanos , L-Lactato Desidrogenase/metabolismo , Masculino , Microscopia Eletrônica de Varredura , Pessoa de Meia-Idade , Propriedades de Superfície , Fatores de Tempo , Adulto JovemRESUMO
We studied the effect of carbon monoxide (60-, 75-, and 90-min exposure) on the expression of antiapoptotic proteins (survivin and Bcl-2) in human blood lymphocytes in the presence of recombinant IL-2 in an apoptosis-inducing dose (0.1 ng/ml). Incubation of cells in atmosphere with carbon monoxide in the presence of recombinant IL-2 was accompanied by accumulation of Bcl-2 protein with simultaneous decrease of survivin content. It was concluded that carbon monoxide plays a role in the dysregulation of apoptosis of human blood lymphocytes Bcl-2 (i.e. CO inhibits the proapoptotic effect of recombinant IL-2).