Assuntos
Artrite Infecciosa , Artrite , Bacteriemia , Infecções Estafilocócicas , Articulação Esternoclavicular , Humanos , Infecções Estafilocócicas/diagnóstico , Infecções Estafilocócicas/tratamento farmacológico , Staphylococcus aureus , Bacteriemia/diagnóstico , Bacteriemia/tratamento farmacológico , Antibacterianos/uso terapêutico , Artrite Infecciosa/diagnóstico , Artrite Infecciosa/tratamento farmacológico , Articulação Esternoclavicular/diagnóstico por imagemAssuntos
Edema/etiologia , Doença de Erdheim-Chester/diagnóstico por imagem , Perna (Membro)/patologia , Idoso , Diagnóstico Diferencial , Doença de Erdheim-Chester/patologia , Humanos , Perna (Membro)/diagnóstico por imagem , Imageamento por Ressonância Magnética , Masculino , Tomografia por Emissão de Pósitrons , Tomografia Computadorizada por Raios XAssuntos
Antibióticos Antituberculose/uso terapêutico , Ascite/metabolismo , Antígeno Ca-125/metabolismo , Neoplasias Peritoneais/diagnóstico , Peritonite Tuberculosa/diagnóstico , Dor Abdominal , Idoso , Diagnóstico Diferencial , Evolução Fatal , Feminino , Febre , Hemorragia Gastrointestinal , Humanos , Neoplasias Peritoneais/patologia , Peritonite Tuberculosa/tratamento farmacológico , Peritonite Tuberculosa/patologia , VômitoRESUMO
In winter 2001, an outbreak of pertussis involving an estimated 75 people occurred among soldiers serving in an infantry regiment of the Israeli Defense Forces (IDF). Nasopharyngeal swabs were obtained from patients and contacts for culture and PCR. Serum samples were obtained and assayed by ELISA for the presence of IgA, IgM and IgG antibodies to a lysate antigen of Bordetella pertussis. The calculated attack rate was 21% based on clinical signs alone (cough lasting 30 days or longer) and 9.5% based on clinical signs with laboratory confirmation (by PCR, IgA or IgM). A high carriage rate was observed; 20% of the asymptomatic and previously symptomatic subjects were PCR-positive for B. pertussis. These findings emphasize the importance of B. pertussis as a causative agent of epidemic respiratory infections in young adults and reveal the occurrence of a significant proportion of pertussis transient carriers during an outbreak of the disease.
Assuntos
Surtos de Doenças , Militares , Coqueluche/epidemiologia , Adulto , Bordetella pertussis/genética , Bordetella pertussis/imunologia , Bordetella pertussis/patogenicidade , Portador Sadio , Ensaio de Imunoadsorção Enzimática , Feminino , Humanos , Imunoglobulina A/análise , Imunoglobulina G/análise , Imunoglobulina M/análise , Israel/epidemiologia , Masculino , Nasofaringe/microbiologia , Vacina contra Coqueluche , Reação em Cadeia da Polimerase , Coqueluche/imunologiaRESUMO
Hairpin and tetrahelical structures of a d(CGG)(n) sequence in the FMR1 gene have been implicated in its expansion in fragile X syndrome. The identification of tetraplex d(CGG)(n) destabilizing proteins (Fry, M., and Loeb, L. A.(1999) J. Biol. Chem. 274, 12797-12803; Weisman-Shomer, P., Naot, Y., and Fry, M. (2000) J. Biol. Chem. 275, 2231-2238) suggested that proteins might modulate d(CGG)(n) folding and aggregation. We assayed human TK-6 lymphoblastoid cell extracts for d(CGG)(8) oligomer binding proteins. The principal binding protein was identified as Ku antigen by its partial amino acid sequence and antigenicity. The purified 88/75-kDa heterodimeric Ku bound with similar affinities (K(d) approximately 1. 8-10.2 x 10(-9) mol/liter) to double-stranded d(CGG)(8).d(CCG)(8), hairpin d(CGG)(8), single-stranded d(CII)(8), or tetraplex structures of telomeric or IgG switch region sequences. However, Ku associated more tightly with bimolecular G'2 tetraplex d(CGG)(8) (K(d) approximately 0.35 x 10(-9) mol/liter). Binding to Ku protected G'2 d(CGG)(8) against nuclease digestion and impeded its unwinding by the tetraplex destabilizing protein qTBP42. Stabilization of d(CGG)(n) tetraplex domains in FMR1 by Ku or other proteins might promote d(CGG) expansion and FMR1 silencing.