Caribbean medicinal plant Argemone mexicana L.: Metabolomic analysis and in vitro effect on the vaginal microbiota.

ETHNOPHARMACOLOGICAL RELEVANCE: Medicinal plants are frequently used in Caribbean traditional medicine as low-cost, culturally relevant treatments for women's health concerns, such as gynecological infections. These plants are typically applied topically, potentially affecting both pathogenic bacteria (e.g., Gardnerella vaginalis) and beneficial vaginal microbes (Lactobacillus spp.). However, few studies have examined the impact of these plants on both beneficial and pathogenic vaginal bacteria. AIM OF THE STUDY: Argemone mexicana, available in New York City and commonly used to treat gynecological infections by immigrants from the Dominican Republic, was investigated for its chemical variation and effects on the vaginal microbiota. We hypothesized that variations in the bioactivity of Argemone mexicana on Gardnerella vaginalis and Lactobacillus spp. are due to differences in antimicrobial compounds across different preparations. MATERIALS AND METHODS: Untargeted and targeted metabolomic analysis using UPLC-qToF-MS and UPLC-TQD-MS were conducted on Argemone mexicana samples collected in New York City. Antimicrobial assays were used to assess the effects of Argemone mexicana samples on beneficial and pathogenic vaginal bacteria. ProGenesis QI and EZinfo were used for metabolomic analysis to link bioactivity with chemometric data. RESULTS: UPLC-qToF-MS and statistical analyses showed that chemical variation correlated with plant tissue type and processing (dry or fresh samples). These differences were evident in antimicrobial screenings, where active plant samples were antimicrobial against pathogenic bacteria only, with no effect on beneficial Lactobacillus. Known antimicrobial benzoquinone alkaloids, such as berberine, were partly responsible for the observed microbiological activity. Berberine exhibited similar inhibition patterns, reduced biofilm formation, and trended towards higher concentration in active samples. CONCLUSIONS: Extracts of Argemone mexicana, a plant used in Caribbean women's health, did not have an effect on beneficial vaginal microbes, but did inhibit pathogenic Gardnerella vaginalis. This antimicrobial activity correlated with the chemical variation of berberine and other related alkaloids across traditional preparations of Argemone mexicana. These results may be relevant for treating gynecological infections, not only with this plant, but other berberine-containing taxa.

Haitian women in New York City use global food plants for women's health.

BACKGROUND: Despite the availability of mainstream biomedical healthcare in New York City (NYC), community-based ethnomedicine practices remain a low-cost, culturally relevant treatment for many immigrants. Previous urban ethnobotany research in NYC has established that several Caribbean communities continue using medicinal plants for women's health after immigration. This study sought to address to what extent: (1) NYC Haitian women continue using medicinal plants for women's health after migration; (2) their plants and the conditions treated were similar to those identified in an earlier survey with NYC immigrants from the Dominican Republic. METHODS: Through an ethnobotanical survey, 100 Haitian women living in NYC and born in Haiti were interviewed about their knowledge of medicinal plants for women's health conditions. Reported species were purchased based on local names in NYC Haitian stores and markets, vouchered, and identified. RESULTS: Nearly all Haitian women (97%) reported using medicinal plants while living in Haiti. Most Haitian women continued using medicinal plants after coming to the USA (83%). The 14% decrease, although significant (z = 3.3; p = 0.001), was mainly due to logistical difficulties with sourcing plants after recent immigration. Popular medicinal plant species reported were primarily global food plants, re-emphasizing the intertwined food-medicine relationship in Caribbean diasporas. Comparison with data from NYC Dominicans identified childbirth and puerperium, gynecological infections, and vaginal cleansing as priority Haitian women's health concerns treated with plants. CONCLUSION: Our findings support the global nature of Caribbean migrant plant pharmacopeia, predominantly centered around food plants and adapted to transnational urban settings. They underscore cultural diversity, dispelling the notion of one uniform traditional knowledge system labeled "Caribbean." The importance of preventative medicine for women's health, particularly the regular consumption of "healthy" foods or teas highlights the role food plants play in maintaining health without seeking treatment for a particular condition. Cross-cultural comparisons with other NYC Caribbean immigrants emphasize the importance of conducting ethnobotanical surveys to ground-truth plant use in the community. Such surveys can also identify culture-specific health priorities treated with these plants. Healthcare providers can leverage these insights to formulate culturally relevant and community-tailored healthcare strategies aligned with Haitian women's health beliefs and needs.

Potential Anti-Alzheimer Properties of Mogrosides in Vitamin B12-Deficient Caenorhabditis elegans.

Vitamin B12 deficiency can lead to oxidative stress, which is known to be involved in neurodegenerative diseases such as Alzheimer's disease (AD). Mogrosides are plant-derived triterpene glycosides that exhibit anti-inflammatory and antioxidant activity in animal cell lines and mouse models. Since amyloid-ß toxicity is known to cause oxidative stress and damage to brain cells, we hypothesized that mogrosides may have a protective effect against AD. In this study, we investigated the potential anti-AD effect of mogrosides in vitamin B12-deficient wild-type N2 and in transgenic CL2355 Caenorhabditis elegans expressing amyloid-ß peptide. Our data indicated that mogrosides have a beneficial effect on the lifespan and egg-laying rate of N2 and vitamin B12-deficient N2 worms. Additionally, the results revealed that mogrosides can effectively delay the paralysis of CL2355 worms as determined by serotonin sensitivity assay. Our analysis showed that mogrosides increase the expression of oxidative protective genes in N2 worms fed with vitamin B12-deficient OP50 bacterium. We conclude that mogrosides may exert preventative rather than curative effects that counteract the detrimental vitamin B12-deficient environment in N2 and CL2355 C. elegans by modulating oxidation-related gene expression.

Caribbean Women's Health and Transnational Ethnobotany.

Caribbean Women's Health and Transnational Ethnobotany. Immigrants from the Dominican Republic (DR) and Haiti are among the top foreign-born communities in New York City (NYC). As people migrate to new countries, they bring their ethnomedical beliefs and practices, and adapt their plant pharmacopoeias. Haiti and the DR share a flora on the island of Hispaniola. In NYC, the flora is limited to what is available in the city. We selected plants for future laboratory research based on ethnobotanical data from two surveys among Dominicans in the DR and NYC, and a Haitian literature review. In both Dominican datasets, gynecological infections were the top women's health condition treated with plants. We identified 10 species for this purpose reported by Dominicans that are also known medicines in Haitian culture, although not yet documented for women's health. Plants for gynecological infections potentially cause dysbiosis of the vaginal microbiota, and may increase rather than prevent disease. There is a public health need to assess traditional medicines for their ability to inhibit pathogenic bacteria, while causing minimal disruption to the vaginal flora. Several species are known antibacterials, but remain to be tested for their efficacy. These results also provide a foundation for a planned ethnobotanical survey among NYC Haitian women. Supplementary Information: The online version contains supplementary material available at 10.1007/s12231-021-09526-3.