Production and characterization of microbial levan using sugarcane (Saccharum spp.) juice and chicken feather peptone as a low-cost alternate medium.

An alternate medium consisting of sugarcane juice (SJ) (Saccharum spp.) and chicken feather peptone (CFP) was employed for microbial synthesis of levan. SJ has considerable amounts of vital minerals, vitamins, and amino acids in addition to its major constituent, sucrose. Meanwhile, CFP is also a rich source of essential nutrients such as amino acids, micro and macro elements. Amino acids present in SJ and CFP, such as glutamic acid, arginine, aspartic acid, asparagine and elements such as Ca, Mg favoured the cell growth and levan production. In this present work, levan was produced using Bacillus subtilis MTCC 441 in five different media, namely, sucrose along with defined nutrients (M1), Sugarcane Juice without nutrients (M2), SJ with defined nutrients (M3), SJ along with chicken feather peptone (M4) and sucrose without nutrient (M5). Alternative nutrient medium using SJ and CFP (M4) showed a promising levan yield of 0.32 ± 0.01 g of levan/g of sucrose consumed, which is 64% of the theoretical levan yield possible. Levan produced was characterized using Nuclear Magnetic Resonance (NMR) and Gel Permeation Chromatography (GPC). There is a change in low molecular weight fractions of levan obtained from SJ and CFP medium compared to the defined medium. Produced levan from the composite medium exhibited strong antioxidant activity and was biocompatible when tested against endothelial cells. The substrate cost was 20% lower than the cost of defined medium. Thus, a composite medium made of SJ and CFP can serve as an alternate low-cost medium for microbial fermentation.

Bioprospecting of aqueous phase from pyrolysis of plant waste residues to disrupt MRSA biofilms.

Methicillin resistant Staphylococcus aureus (MRSA) infections have increased at an alarming rate, recently. In India, stubble burning and air pollution due to the burning of agricultural and forest residues have also increased over the past decade causing environmental and health hazards. This work evaluates the anti-biofilm property of the aqueous phase obtained from pyrolysis of wheat straw (WS AQ) and pine cone (PC AQ) against an MRSA isolate. The WS AQ and PC AQ compositions were determined by GC-MS analysis. The minimum inhibitory concentration was found to be 8% (v v-1) and 5% (v v-1) for WS AQ and PC AQ, respectively. The eradication of biofilms was performed on hospital contact surfaces namely, stainless steel and polypropylene and found to be 51% and 52% for WS AQ and PC AQ, respectively. Compounds identified from the aqueous phase of WS and PC docked against AgrA protein showed good binding scores.

Waste to drugs: identification of pyrolysis by-products as antifungal agents against Cryptococcus neoformans.

The fungi, Cryptococcus neoformans cause major infections such as cryptococcal meningitis and cryptococcosis. Therefore, we explored the use of Thioredoxin reductase (Trr1) from C. neoformans as a gene target for the development of novel antifungal agents. Trr1 plays an essential role in the survival in the oxidative environment of macrophages and is important for the virulence of C. neoformans. During the thermochemical conversion (pyrolysis) of lignocellulosic biomass (LCB), a cocktail of compounds is produced by the decomposition and degradation. In general, LCB-derived cocktail of compounds is a rich source of aromatic compounds that have been shown to be antifungal in nature. Usually, the aqueous phase produced during biomass pyrolysis is generally regarded as waste. Here, we used Parthenium hysterophorus biomass as the antifungal source and obtained the aqueous phase after pyrolysis. Using GC-MS analysis of the aqueous phase collected from P. hysterophorus biomass revealed the presence of a large number of aromatic and organic compounds. Using virtual screening, the compounds present in the aqueous phase were docked against Trr1 using GLIDE. Two promising candidates were analyzed further by performing molecular dynamics simulation using GROMACS, to establish stable interactions. We validated the computational results with clustering analysis. We report that 2,4-Di-tertbutyl phenol and 1H-Pyrazole, 4-ethyl-3,5-dimethyl have a potent antifungal property and we postulate that they could be a potent antifungal agent against Trr1 of C. neoformans.

Photocatalytic degradation of Reactive Black dye using ZnO-CeO2 nanocomposites.

This study presents the photocatalytic efficiency of ZnO-CeO2 nanocomposites for the degradation of a model Reactive Black (RB) dye. Nano-CeO2 was synthesized using cerium nitrate precursor solution via chemical precipitation. Synthesized nano-CeO2 was mixed with ZnO nanoparticles in different mass ratios to obtain ZnO-CeO2 heterojunction photocatalyst. The morphology of the nanocomposites was examined using transmission electron microscope (TEM). X-ray diffraction patterns of the CeO2 corresponded well with (1 1 1) plane of cubic-phase CeO2. The band gap of the ZnO-CeO2 nanocatalyst synthesized was determined to be 3.08 eV, which was lower than that of the pristine CeO2 and ZnO powders, respectively. The results indicate that 1:1 wt. ratio ZnO-CeO2 nanocomposite provides about 85% RB degradation within 90 min under UV light under alkaline pH conditions. Degradation rate of RB dye achieved with ZnO-CeO2 nanocomposite was almost 1.5 times greater than that obtained with pristine ZnO. Increasing CeO2 ratio beyond 1:1 wt. ratio did not significantly increase RB degradation. The results demonstrate that addition of CeO2 to ZnO results in lowering its band gap energy and aids charge carrier separation resulting in enhanced oxidation of RB dye under UV light.

Stoichiometric Analysis and Production of Bacterial Cellulose by Gluconacetobacter liquefaciens using Borassus flabellifer L. Jaggery.

The objective of the work is to examine the potential utilization of Palmyra palm jaggery (PPJ) for the enhancement of bacterial cellulose (BC) production by Gluconacetobacter liquefaciens. To evaluate the culturing condition, the production of BC fermentation was carried out in batch mode using different carbon sources namely glucose, sucrose and PPJ. PPJ in the HS medium (PHS medium) resulted maximum concentration of BC (14.35 ± 0.18 g/L) under shaking condition than other carbon sources in HS medium. The influence of different medium variables including initial pH and nitrogen sources on BC production was investigated using PHS medium under shaking condition. The maximum BC concentration of 17.79 ± 2.4 g/L was obtained in shaking condition at an initial pH of 5.6 using yeast extract as nitrogen source. Stoichiometric equation for the cell growth and BC synthesis was developed using elemental balance approach. The metabolic heat of reaction (40 kcal generated per liter of medium) was evaluated using electron balance approach. Based on the process economic analysis and the yield of BC during the fermentation, PHS medium without nitrogen source could be a promising cost-effective nutrient than HS medium. Thermal stability, crystallinity index and structural characterizations of produced BC using PPJ medium were evaluated using TGA, XRD and FTIR and the obtained results were compared with HS medium containing glucose and sucrose.

Waste tire particles as efficient materials towards hexavalent chromium removal: Characterisation, adsorption behaviour, equilibrium, and kinetic modelling.

The growing demand for vehicles is increasing every year, and this paves the way for the environmental problem for the generation of the waste tire (WT) and associated disposal after their end life. On the other direction, effluent from tannery and electroplating industries contented with a higher concentration of chromium, which is a toxic pollutant, induces mutation effects. In this work, the approach of reutilization of waste tires as adsorbent is reported towards the sequestrations of hexavalent chromium (Cr(VI)) from the simulated system. The waste tire sample is activated using orthophosphoric acid (H3PO4) and utilized for adsorption studies. Adsorption studies are performed with native tire sample (NTP) and activated tire sample (ATP). About 96.5% of Cr(VI) adsorption efficiency was attained using ATP and at the optimal adsorption solution pH 2.0. The adsorbent performed relatively well in wide a range of initial solution pH tested in the present research. The experimental data analysis of Cr(VI) adsorption onto NTP and ATP revealed the best fit with the kinetic model of pseudo-second-order. Further, the equilibrium data were analysed using two-parameter isotherms, relatively the Langmuir isotherm best represented in both NTP and ATP. ATP demonstrated with higher Cr(VI) removal performance with an adsorption capacity of 102.90 mg/g, according to the Langmuir model. The proposed idea of utilizing waste tire particles as adsorbents in the treatment of Cr(VI) contaminated water offers valuable guidance towards further investigations in the directions of dynamic adsorption and effluent treatment.

Production of bacterial cellulose using Gluconacetobacter kombuchae immobilized on Luffa aegyptiaca support.

The present work report for the first time on the production of bacterial cellulose (BC) using natural loofa sponge (Luffa aegyptiaca) as a scaffold for the immobilization of Gluconacetobacter kombuchae. Bacterial cellulose (BC) are recently gained more attention in several fields including biological and biomedical applications due to their outstanding physico-chemical characteristics including high thermal stability, easy biodegradability, good water holding capacity, high tensile strength, and high degree of polymerization. The increase in requirement of alternative method for the enhancement of BC production under economical aspect develops a positive impact in large scale industries. In this study, Luffa aegyptiaca (LA) was introduced in a separate fermentation medium so as to enhance the concentration of BC production by Gluconacetobacter kombuchae. Different process/medium parameters such as initial pH, static/shaking condition, inoculum size, nitrogen source, C/N ratio, supplements (ethanol and acetic acid) were analysed for the production of bacterial cellulose using LA support. The maximum yield of BC was obtained using following condition: culturing condition -shaking; initial pH - 5.5; nitrogen source- yeast extract, C/N ratio - 40 and supplement-ethanol. The characterization of the BC was examined using Fourier Transform Infra-Red spectroscopy and thermo gravimetric analysis. The biofilm formation on the surface of LA was examined by SEM photographs. Thus, implementation of LA as a support in shaking fermentation under suitable medium/process variables enhanced the BC production.

Remediation of textile effluents for water reuse: Decolorization and desalination using Escherichia fergusonii followed by detoxification with activated charcoal.

Textile effluents contain high levels of pollutants of different categories like dyes, metal salts, acids, bases and microorganisms. Remediation of textile effluents is often challenging because of its composition, which also varies between dyeing units. In this study, we demonstrate the novel use of a waste-water bacterium, Escherichia fergusonii, in the effective remediation of textile effluents. The bacteria application efficiently caused a reduction of color (98.4%), total dissolved solids (75%), sulphates (87%), bicarbonates (83%), chlorides (64%), calcium (84%), and chemical oxygen demand (81%) of the textile effluents. The bacteria-treated effluents were further disinfected and detoxified by treating with rice husk activated charcoal. After the charcoal treatment, the chemical oxygen demand decreased further by 11.5% and biochemical oxygen demand decreased by 85%. The effluents remediated using the two-step process were subjected to toxicity assays using zebrafish (Danio rerio) model. The textile effluents treated using Escherichia fergusonii, followed by activated charcoal were found to be non-toxic and suitable for reuse for domestic applications. Thus, we present here, a simple, less energy-intensive, economic, two-step process as a complete solution for textile effluent treatment. The results of this investigation can be used to simplify the remediation process of textile effluents in common treatment plants as well as the individual dyeing units.

Studies on solvent precipitation of levan synthesized using Bacillus subtilis MTCC 441.

Levan is a water soluble biopolymer widely used in food, pharma, personal care and aquaculture industries. In this work, levan was synthesized by Bacillus subtilis MTCC 441 using sucrose as a sole carbon source. Effects of pH, sucrose concentration, nitrogen source, nitrogen concentration, inoculum size and agitation speed on levan production were studied. Yeast extract (YE) was found to be the best nitrogen source. Sucrose concentration - 100 g/L, pH - 7, YE concentration - 2 g/L, inoculum size 10% (v/v) and RPM - 150 were found to be optimal values for levan production. Effects of precipitation pH (3-12), choice of solvent (ethanol, isopropanol, acetone, and methanol) and supernatant to solvent ratio (1:1 to 1:6) on levan yield were also studied. Isopropanol resulted in maximum levan recovery among the four solvents considered. Optimal pH and supernatant to solvent ratio for levan precipitation were found to be 11 and 1:5, respectively. Corresponding levan yield was 0.395 g/g of sucrose supplied. The product obtained was characterized using FTIR, 1H-NMR, 13C-NMR, and GPC. The cytotoxicity of the precipitated levan was studied on EA.hy926 cell line using MTT assay and the compound was proven to be non-toxic to the cells.

Response surface methodology: optimisation of antifungal bioemulsifier from novel Bacillus thuringiensis.

An antifungal bioemulsifier compound was produced from a novel strain of Bacillus thuringiensis pak2310. To accentuate the production and as the first step to improve the yield, a central composite design (CCD) was used to study the effect of various factors like minimal salts (1X and 3X), glycerol concentration (2% and 4%), beef extract concentration (1% and 3%), and sunflower oil concentration (2% and 4%) on the production of bioemulsifier molecule and to optimize the conditions to increase the production. The E 24 emulsification index was used as the response variable as the increase in surfactant production was seen to be proportional to increased emulsification. A quadratic equation was employed to express the response variable in terms of the independent variables. Statistical tools like student's t-test, F-test, and ANOVA were employed to identify the important factors and to test the adequacy of the model. Under optimum conditions (1X concentration of minimal salts (MS), 2.6% glycerol (v/v), 1% beef extract (w/v), and 2% sunflower oil (v/v)) a 65% increase in yield was produced.