Risk Factors and Methods for Predicting Ovarian Hyperstimulation Syndrome (OHSS) in the in vitro Fertilization.

Ovarian hyperstimulation syndrome (OHSS) is the most severe and life-threatening complication of assisted reproductive technologies (ART). OHSS is based on an excessive ovarian response to ovarian stimulation; however, the pathogenesis has not been fully understood yet. The most serious complications of OHSS are thromboembolic complications and ovarian torsion. The current study describes the risk factors for the development of ovarian hyperstimulation syndrome and proposes a method for specific prediction of this syndrome. This study was designed to investigate 671 therapeutic cycles in the IVF program during 2009-2018. All patients were divided into two groups. Group one (n=56) included women who developed OHSS during the IVF procedure. Group two (n=615) consisted of women who did not have this complication during the IVF procedure. All the observation and examination outcomes were entered into a specially developed questionnaire, and then into a Microsoft Excel spreadsheet. The data were processed by variable statistics using Statistica 10.0. Analyzing of the recorded data revealed that the rate of OHSS was higher in the group of younger women, aged 30.76±3.67 years, in comparison with those aged 32.78±4.40 years in the group of patients without OHSS (p<0.05). The analysis of the initial phase of the reproductive system has confirmed that the group of patients with OHSS had a higher level of prolactin, 462.84±191.56 mIU/L in comparison with 363.43±187.84 mIU/L, which corresponded to the group of women without OHSS (p<0.05). In our results, 7.15±1.04% of cases with OHSS had obesity, while of the patients from the group without OHSS suffered from it (p<0.05). OHSS is the most severe iatrogenic complication of ART, therefore it is extremely important to consider its risk factors and take timely preventive measures. This study has established a high relationship between the studied risk factors and ovarian hyperstimulation syndrome and proposed a model for predicting this syndrome.

[Polymorphic locus rs1061624 of the ТNFR2 gene is associated with the development of arterial hypertension in males].

Aim To study the involvement of cytokine polymorphous loci in development of arterial hypertension (AH) in men from the Central Black Earth region of Russia.Materials and methods 821 men were evaluated, including 564 patients with AH and 257 individuals of the control group. Analysis of 8 cytokine mononucleotide polymorphisms (MNP) was performed using the real-time polymerase chain reaction with TagMan probes. Statistical analysis was performed with the STATISTICA (v.10.0) and PLINK (v.1.06) software. The regulatory potential of MNP was analyzed with the HaploReg (v.4.1) service (http://archive.broadinstitute.org).Results The rs1061624 ТNFR2 polymorphous locus was associated with development of AH in men in recessive (odd ratio (OR), 0.33; 95 % confidence interval (CI): 0.18-0.61, рperm=0.0004) and additive (OR, 0.50, 95 % CI: 0.34-0.74, рperm=0.0006) genetic models and exerted a protective effect in development of AH. The rs1061624 MNP of the ТNFR2 gene has a regulatory significance; it is located in the DNA sites hypersensitive to the action of DNAase 1 and in binding sites for transcriptional factors and histones that mark enhancers and promoters in different organs and tissues.Conclusion The rs1061624 ТNFR2 gene polymorphism is involved in the development of AH in men of the Central Black Earth region of Russia.

[STUDY OF THE INFLUENCE OF MINERAL FERTILIZERS ON THE NEONATAL MORBIDITY RATE IN INFANTS WITH CONGENITAL MALFORMATIONS (CM) IN THE BELGOROD REGION].

Results of the performed study showed that the use of mineral fertilizers for crops increases the risk of morbidity in neonates with congenital malformations (CM). P-elevated levels of relative environmental risk is established for: anomalies of the musculoskeletal system, multiple congenital malformations, the CM as a whole. Maximum environmental risk in the conditions of high load offertilizers on farmland is established for congenital abnormalities of the development of reproductive system in newborn infants (RR = 2,76).

[Multifactorial analysis of the impact of pesticides on the gynecologic rate in adolescents in the Belgorod Region].

In the course of the performed study the significant influence of pesticides introduced into arable farmland, on the frequency of gynecological morbidity in adolescents in 21 districts of the Belgorod region was established. With increasing pesticide load on the soil the prevalence of gynecological pathology in adolescents in rural areas is raising.

[Influence of geliophysical factors on neonatal morbidity in the Belgorod Region].

The study of the influence of geliophysical factors on neonatal morbidity in the Belgorod Region in 2000 to 2008 established a rise in the rate of congenital malformations, fetal hypoxia and asphyxia, prematurity, and overall neonatal morbidity during a period of high solar activity.

[Examining the impact of solar activity on the incidence of congenital malformations].

The investigation revealed statistically significant positive and negative correlations between the impact of solar activity (SA) and the incidence of congenital malformations (CM). As SA decreased, the incidence of multiple CM, defects of the musculoskeletal system, and those as a whole increased, but among the nosological entities, there was a rise in the prevalence of diaphragmatic hernia and omphalocele. At the same time, increased SA determines the higher incidence of abnormalities of the central nervous system, sensory organs, face and neck, anencephalies, and cleft lips. The maximum influence of SA was established for CM of the skin (56.25%), face and neck (53.46%); and among the nosological entities of CM that was for the cleft lip (60.65%) and cerebrospinal hernia (46.51%).

[Impact of man-made atmospheric pollution on the incidence of congenital malformations].

S u m mary. The paper presents the results of studying the qualitative and quantitative representation of anthropogenic pollutants in the ambient air environment of Belgorod and evaluating their impact on the prevalence of neonatal congenital malformations. The level of atmospheric pollutant emissions is shown to vary by an average of 4 to 10 times in some areas. The vast majority (91.7%) of pollutants form clusters (n = 11), suggesting their combined influence. Each group of clusters includes 2 to 9 pollutants (median 4.64 pollutants). Atmospheric pollutants have been ascertained to adversely affect the prevalence of congenital malformations in children. A multiple regression equation has been derived to predict the frequency of congenital malformations, by taking into account the qualitative and quantitative spectrum of atmospheric pollutants.