Ten years of directing seeding restoration in the Brazilian savanna: Lessons learned and the way forward.

Savannas and grasslands have lost almost 50% of their original cover worldwide. Therefore, the development of methods and information on open-canopy ecosystem restoration is urgent for the inclusion of these ecosystems into global and regional priorities. In the Brazilian savanna, the most diverse savanna in the world, restoration efforts focused on open ecosystems have been virtually absent, but have increased in the last 10 years. Such efforts are frequently threatened by invasive exotic grasses (IEG) that invade and dominate areas excluding native species, oftentimes aided by altered soil conditions. Long-term studies of savanna restoration trajectories are rare. In this study, we surveyed 22 savanna restoration areas established two to ten years before the study with similar restoration methods to assess their current status. We show that the current restoration methods are successful in establishing native species and allowing species turnover but they are threatened by IEG. Restoration success varies and is affected by soil conditions, IEG landscape cover and post-sowing weeding. Despite that, the simultaneous introduction of different plant functional groups allows turnover from fast to slow-growing plants. Establishing savanna native species is possible at an operational scale with current knowledge and techniques. However, native species establishment fails to prevent IEG reinfestation, which needs to be managed in restoration efforts in the Brazilian savanna.

Anti-mycotoxin feed additives: effects on metabolism, mycotoxin excretion, performance, and total tract digestibility of dairy cows fed artificially multi-mycotoxin-contaminated diets.

The aim of this study was to evaluate the effects of different anti-mycotoxin feed additives on the concentration of mycotoxins in milk, urine, and blood plasma of dairy cows fed artificially multi-mycotoxin-contaminated diets. Secondarily, performance, total-tract apparent digestibility of nutrients, and blood parameters were evaluated. Twelve multiparous cows (165 ± 45 d in milk, 557 ± 49 kg body weight, and 32.1 ± 4.57 kg/d milk yield at the start of the experiment) were blocked according to parity, milk yield, and days in milk and used in a 4 × 4 Latin square design experiment with 21-d periods, where the last 7 d were used for sampling and data analysis. Treatments were: 1) Mycotoxin group (MTX), basal diet (BD) without anti-mycotoxin feed additives; 2) Hydrated sodium calcium aluminosilicate (HSCA), HSCA added to the BD at 25g/cow/d; 3) Mycotoxin deactivator 15 (MD15), MD (Mycofix® Plus, dsm-firmenich) added to the BD at 15 g/cow/d; and 4) Mycotoxin deactivator 30 (MD30), MD added to the BD at 30 g/cow/d. Cows from all treatments were challenged with a blend of mycotoxins containing 404 µg aflatoxins B1 (AFB1), 5,025 µg deoxynivalenol (DON), 8,046 µg fumonisins (FUM), 195 µg T2 toxin (T2), and 2,034 µg of zearalenone (ZEN) added daily to the BD during the last 7 d of each period. Neither performance (milk yield and composition) nor nutrient digestibility was affected by treatments. All additives reduced aflatoxin M1 (AFM1) concentration in milk, whereas MD15 and MD30 group had lower excretion of AFM1 in milk than HSCA. DON, FUM, T2, or ZEN were not detected in milk of MD15 and MD30. Concentrations in milk of DON, FUM, T2, and ZEN were similar between MTX and HSCA. Except for AFM1, none of the analyzed mycotoxins were detected in urine of MD30 group. Comparing HSCA to MD treatments, the concentration of AFM1 was greater for HSCA, whereas MD30 was more efficient at reducing AFM1 in urine than MD15. AFM1, DON, FUM, and ZEN were not detected in the plasma of cows fed MD30, and DON was also not detected in MD15 group. Plasma concentration of FUM was lower for MD15, similar plasma FUM concentration was reported for HSCA and MTX. Plasma concentration of ZEN was lower for MD15 than MTX and HSCA. Serum concentrations of haptoglobin and hepatic enzymes were not affected by treatments. Blood concentration of sodium was lower in HSCA compared with MD15 and MD30 groups. In conclusion, the mycotoxin deactivator proved to be effective in reducing the secretion of mycotoxins in milk, urine, and blood plasma, regardless of the dosage. This reduction was achieved without adverse effects on milk production or total-tract digestibility in cows fed multi-mycotoxin-contaminated diets over a short-term period. Greater reductions in mycotoxin secretion were observed with full dose of MD.

Dietary supplementation with live or autolyzed yeast: Effects on performance, nutrient digestibility, and ruminal fermentation in dairy cows.

This study was conducted to evaluate the effects of live or autolyzed yeast supplementation on dairy cow performance and ruminal fermentation. Two experiments were conducted to evaluate performance, feed sorting, total-tract apparent digestibility of nutrients, purine derivatives excretion, N utilization, ruminal fermentation, and the abundance of specific bacterial groups in the rumen. In experiment 1, 39 Holstein cows (171 ± 40 DIM and 32.6 ± 5.4 kg/d milk yield) were blocked according to parity, DIM, and milk yield and randomly assigned to the following treatments: control (CON); autolyzed yeast fed at 0.625 g/kg DM (AY; Levabon, DSM-Firmenich); or live yeast fed at 0.125 g/kg DM (LY; Vistacell, AB Vista). Cows were submitted to a 2-wk adaptation period followed by a 9-wk trial. In experiment 2, 8 ruminal cannulated Holstein cows (28.4 ± 4.0 kg/d milk yield and 216 ± 30 DIM), of which 4 were multiparous and 4 were primiparous, were blocked according to parity and enrolled into a 4 × 4 Latin square experiment with 21-d periods (the last 7 d for sampling). Cows within blocks were randomly assigned to treatment sequences: control (CON), LY (using the same product and dietary concentration as described in experiment 1), AY, or autolyzed yeast fed at 0.834 g/kg DM (AY2). In experiments 1 and 2, nutrient intake and total-tract apparent digestibility were not affected by treatments. Sorting for long feed particles (>19 mm) tended to be greater in cows fed yeast supplements than CON in experiment 1. Efficiency of N conversion into milk N was increased when feeding yeast supplements in experiment 1, and 3.5% FCM yield tended to be greater in cows fed yeast supplements than CON. Feed efficiency was increased when yeast supplements were fed to cows in relation to CON in experiment 1. In experiment 2, yield of FCM and fat were greater in cows fed yeast supplements compared with CON. Uric acid concentration and output in urine were increased when feeding yeast supplements when compared with CON. Neither ruminal pH nor total VFA were influenced by treatments. The current study did not reveal treatment differences in ruminal abundance of Anaerovibrio lipolytica, the genus Butyrivibrio, Fibrobacter succinogenes, Butyrivibrio proteoclasticus, or Streptococcus bovis. Yeast supplementation can increase feed efficiency without affecting nutrient intake and digestibility, ruminal VFA concentration, or ruminal abundance of specific bacterial groups. Supplementing live or autolyzed yeast, regardless of the dose, resulted in similar performance.

Enneothrips Hood: a Neotropical genus of leaf-feeding thrips (Thysanoptera: Thripidae) with generic diagnosis and four new species.

Enneothrips is a lineage of leaf-feeding thrips conventionally placed in the Anaphothrips genus-group (Thripinae) and exclusively found in the Neotropics. Here we describe E. amazonicus sp. n., E. fulbrightae sp. n. and E. manauara sp. n. from the Brazilian Amazon and E. atlanticus sp. n. from the Brazilian Atlantic Forest, together with an illustrated key to all ten species now recognized in the genus.

Radioactive gold nanoparticles coated with BSA: A promising approach for prostate cancer treatment.

Background: Nanotechnology has revolutionized medicine, especially in oncological treatments. Gold nanoparticles (AuNPs) stand out as an innovative alternative due to their biocompatibility, potential for surface modification, and effectiveness in radiotherapeutic techniques. Given that prostate cancer ranks as one of the leading malignancies among men, there's a pressing need to investigate new therapeutic approaches. Methods: AuNPs coated with bovine serum albumin (BSA) were synthesized and their cytotoxicity was assessed against prostate tumor cell lines (LNCaP and PC-3), healthy prostate cells (RWPE-1), and endothelial control cells (HUVEC) using the MTS/PMS assay. For in vivo studies, BALB/C Nude mice were employed to gauge the therapeutic efficacy, biodistribution, and hematological implications post-treatment with BSA-coated AuNPs. Results: The BSA-coated AuNPs exhibited cytotoxic potential against PC-3 and LNCaP lines, while interactions with RWPE-1 and HUVEC remain subjects for further scrutiny. Within animal models, a diverse therapeutic response was observed, with certain instances indicating complete tumor regression. Biodistribution data emphasized the nanoparticles' affinity towards particular organs, and the majority of hematological indicators aligned with normative standards. Conclusions: BSA-coated AuNPs manifest substantial promise as therapeutic tools in treating prostate cancer. The present research not only accentuates the nanoparticles' efficacy but also stresses the imperative of optimization to ascertain both selectivity and safety. Such findings illuminate a promising trajectory for avant-garde therapeutic modalities, holding substantial implications for public health advancements.

Extraction of high methoxyl pectin from unripe waste Ponkan mandarine (Citrus reticulata Blanco cv. Ponkan) with an eco-friendly solvent.

The aqueous batch extraction of pectin from unripe Ponkan mandarin was evaluated for potential application in the food industry. A small central composite design with 4 variables (temperature, volume to mass ratio, pH, and mean particle size) and 3 levels was applied for pectin extraction optimization. Also, the kinetic of the pectin yield extraction was investigated at temperatures 70-90 °C, stirring rate of 100-700 rpm, ultrasound pretreatment system, and modeling using four mathematical models. The pectin extraction process was evaluated by yield of pectin and esterification degree. FTIR, TGA, and DTA were performed to evaluate the quality of pectin obtained. The small central composite design demonstrated that temperature and volume to mass ratio were significant variables, and the highest yield of pectin extraction was 11.62 % obtained at temperature and volume to molar ratio of 81.9 °C and 33.9 v/w %, respectively. Besides, the esterification degree showed higher than 70 % for all extraction conditions, suggesting high methoxyl pectin. The kinetics showed the stirring rate and the ultrasound pretreatment did not cause any significant alteration, while high temperatures proved to be beneficial to the rate and the yield of the pectin extraction. The best fit was provided by Fick's law, suggesting the extraction process is limited by internal mass transfer. FTIR showed the functional groups expected for pectin, and TGA and DTA indicated that the pectin obtained is proper for most food products, as only above 200 °C the pectin should degrade.

Mapping the regeneration potential of native vegetation in cultivated pastures of the Brazilian Cerrado.

The Brazilian Cerrado is a hotspot of biodiversity conservation and an important global agricultural region. Cultivated pastures under different degradation levels are dominant in the landscape and are being targeted for sustainable agricultural intensification and restoration of native vegetation. In this study, we classified the cultivated pastures of the Brazilian Cerrado according to their potential for natural regeneration, based on field surveys and environmental predictors. We surveyed the native vegetation cover in 186 plots distributed along 93 cultivated pastures. The environmental predictors considered in this study were the proportion of sand in the soil, cation exchange capacity, climate water deficit, pasture age, slope, and pasture vigor index. We then applied the Random Forest regression algorithm to predict and map the cultivated pastures according to their potential for natural regeneration in the 19 Cerrado ecoregions. The potential for natural regeneration was classified into low (< 30% of native plant cover), medium (30-50%), and high (> 50%). Our prediction explained 75% of the data variability. Most of the cultivated pastures presented medium potential for natural regeneration (57%), while 31% and 12% presented high and low potentials, respectively. Cultivated pastures in ecoregions with high mechanization, due to their high water availability and extensive flat terrains, presented low potential for natural regeneration. This first attempt to map the potential for natural regeneration in the cultivated pastures of the Brazilian Cerrado can be used as a proxy for planning low-cost and predictable restoration or environmentally sustainable intensification in this major type of land use found in this biome.

Comparison of topical 0.03% tacrolimus and homologous injectable platelet-rich plasma in the treatment of keratoconjunctivitis sicca in dogs.

Background and Aim: Keratoconjunctivitis sicca (KCS) is predominantly an immune-mediated chronic inflammatory ocular disease that is commonly diagnosed in dogs. This study aimed to compare the conventional use of topical immunosuppressant tacrolimus 0.03% eye drops and a new therapy injectable homologous platelet-rich plasma (HPRP) into the third eyelid gland and inferior and superior palpebral conjunctiva of dogs with KCS. Materials and Methods: A total of 66 eyes from 33 dogs were evaluated. The eyes were divided into three equal groups: Negative control group, tacrolimus group (TG), and homologous platelet-rich plasma group (HPRPG). The animals were evaluated using the Schirmer's tear test-1 (STT-1), osmolarity test (OT), strip meniscometry test (SMT), tear film break-up test (TBUT), fluorescein test, lissamine green test (LGT), and cytological and histopathological analyses. Results: In TG, there was a significant increase (p < 0.05) in the STT-1 and SMT values, and goblet cell count in the palpebral conjunctiva by the end of the study. In HPRPG, 36% (four dogs) received three applications, 55% (six dogs) received two applications, and 9% (one dog) received one application before the initial ocular signs improved. There was a significant decrease (p < 0.05) in the lymphocyte and neutrophil counts of the palpebral conjunctiva in HPRPG than in TG. Both groups showed equivalent improvements in TBUT, OT, and LGT values. Conclusion: Tacrolimus 0.03% eye drops were more efficient than HPRP in increasing tear production and the number of goblet cells. However, injectable HPRP was more efficient than tacrolimus in decreasing the number of conjunctival inflammatory cells. Treatment with injectable HPRP requires an average of two to three applications, is safe and feasible, and can be used as a cheaper alternative or as an adjuvant to conventional treatment with topical immunosuppressants.

Factors affecting savanna and forest regeneration in pastures across the cerrado.

The Cerrado region comprises the world's most biodiverse savanna and the largest cultivated pastures for cattle in Brazil. Forty percent of these pastures are unproductive or degraded, with bare soil and native vegetation increasingly replacing exotic forage grasses. This study sought to investigate the regeneration of native vegetation in the pastures of the Cerrado and to evaluate the contribution of biophysical, land management, and landscape attributes to this process. Across the Cerrado, we analyzed pasture plant communities and the attributes of pasture management intensification, fire events, landscape native vegetation cover, and climate and soil types of 93 active pastures and 15 abandoned pastures. For the abandoned pastures, time since abandonment was an additional variable. On actively cultivated pastures, savanna regeneration varied from 0 to 70%, with a diversity of herbs and woody species. Pasture management was the main predictor of savanna regeneration on cultivated pastures. On abandoned pastures, time since abandonment was the main predictor. Exotic grass cover had a strong negative relationship with savanna regeneration and they were present even in pastures abandoned for 44 years. Our study reveals the potential of natural regeneration of the Cerrado and its particular predictors. The occurrence of pastures with high natural regeneration indicates that national policies can promote native vegetation restoration and silvopastoral systems with predictable, low cost implementation.

Landscape-scale forest cover drives the predictability of forest regeneration across the Neotropics.

Abandonment of agricultural lands promotes the global expansion of secondary forests, which are critical for preserving biodiversity and ecosystem functions and services. Such roles largely depend, however, on two essential successional attributes, trajectory and recovery rate, which are expected to depend on landscape-scale forest cover in nonlinear ways. Using a multi-scale approach and a large vegetation dataset (843 plots, 3511 tree species) from 22 secondary forest chronosequences distributed across the Neotropics, we show that successional trajectories of woody plant species richness, stem density and basal area are less predictable in landscapes (4 km radius) with intermediate (40-60%) forest cover than in landscapes with high (greater than 60%) forest cover. This supports theory suggesting that high spatial and environmental heterogeneity in intermediately deforested landscapes can increase the variation of key ecological factors for forest recovery (e.g. seed dispersal and seedling recruitment), increasing the uncertainty of successional trajectories. Regarding the recovery rate, only species richness is positively related to forest cover in relatively small (1 km radius) landscapes. These findings highlight the importance of using a spatially explicit landscape approach in restoration initiatives and suggest that these initiatives can be more effective in more forested landscapes, especially if implemented across spatial extents of 1-4 km radius.

Emergence of Wigner oscillations in a model of real time cooling process: a time-dependent density-functional theory approach.

Friedel and Wigner oscillations are well known phenomena occurring in quantum systems. Specifically, in a system composed byNconfined fermions, the former are characterized by the presence ofN/2peaks in the density distributions, whereas the last byNpeaks. Here, we considerN = 2 fermions harmonically confined in one-dimensional quantum dots. It is known that the transition from the Friedel to the Wigner oscillations is induced by the increment of interaction between the fermions. The increment of temperature, on the other hand, acts on eliminating the oscillations. In this context, by employing a time-dependent density-functional theory formalism, we here obtain the emergence of Wigner oscillations in a model which simulates a real time cooling process.

Ecological integrity of tropical secondary forests: concepts and indicators.

Naturally regenerating forests or secondary forests (SFs) are a promising strategy for restoring large expanses of tropical forests at low cost and with high environmental benefits. This expectation is supported by the high resilience of tropical forests after natural disturbances, yet this resilience can be severely reduced by human impacts. Assessing the characteristics of SFs and their ecological integrity (EI) is essential to evaluating their role for conservation, restoration, and provisioning of ecosystem services. In this study, we aim to propose a concept and indicators that allow the assessment and classification of the EI of SFs. To this end, we review the literature to assess how EI has been addressed in different ecosystems and which indicators of EI are most commonly used for tropical forests. Building upon this knowledge we propose a modification of the concept of EI to embrace SFs and suggest indicators of EI that can be applied to different successional stages or stand ages. Additionally, we relate these indicators to ecosystem service provision in order to support the practical application of the theory. EI is generally defined as the ability of ecosystems to support and maintain composition, structure and function similar to the reference conditions of an undisturbed ecosystem. This definition does not consider the temporal dynamics of recovering ecosystems, such as SFs. Therefore, we suggest incorporation of an optimal successional trajectory as a reference in addition to the old-growth forest reference. The optimal successional trajectory represents the maximum EI that can be attained at each successional stage in a given region and enables the evaluation of EI at any given age class. We further suggest a list of indicators, the main ones being: compositional indicators (species diversity/richness and indicator species); structural indicators (basal area, heterogeneity of basal area and canopy cover); function indicators (tree growth and mortality); and landscape proxies (landscape heterogeneity, landscape connectivity). Finally, we discuss how this approach can assist in defining the value of SF patches to provide ecosystem services, restore forests and contribute to ecosystem conservation.

Systematics and species recognition in Oneilliella Wilson (Thripidae: Panchaetothripinae).

Oneilliella is a poorly sampled Panchaetothripinae genus with two described species based on very few specimens. Here we provide further information about the genus and describe three species: O. chicoi sp. n. and O. wanessae sp. n. from Brazil, and O. tica sp. n. from Costa Rica. We discuss the relationships between Oneilliella and other members of Panchaetothripinae based on morphology. The Indian species Oneilliella shivii Singha et al. requires further study as it is probably not congeneric with the Neotropical lineage recognized here. An illustrated key to the five Oneilliella species is provided.

Is a diet of Planococcus citri nymphs and adults suitable for Chrysoperla externa for use in biological control?

ABSTRACT Several studies have shown that the survival and reproduction of lacewings varies depending on the type of prey they consumed, especially during the first instar. In this study, we evaluated the effect of a diet consisting of Ephestia kuehniella (EK) and/or Planococcus-citri (PC) eggs offered to first instar Chrysoperla externa on subsequent development and survival. Larvae supplied only with P. citri (PC) nymphs had extended immature development, low survival, adults with lower weight and longevity, low fecundity and egg viability, and lower net reproduction rate (R0). Larvae submitted to the treatment EK+PC responded positively to the diet, showing results similar to those obtained in the control-EK treatment. Therefore, this mealybug supports suitable development and reproduction of C. externa when it is provided to second instar larvae after they fed on E. kuehniella eggs as first instars. As far as we know, this is the first report that demonstrates the effect of natural prey and alternatives on different larval stages of lacewings. This information is valuable in developing protocols for use of C. externaas a pest control agent.

Relationships between soil properties and species establishment in the restoration of mined soils in the Cerrado biome.

Restoring the ecosystems of the Cerrado biome is challenging considering the diversity of phytophysiognomies present in the biome, some of which are composed of species from different strata (herbaceous, shrubby, and arboreal), which increases the complexity of restructuring the floristic composition. Other factors was involved, such as soil quality, which directly influences the success of restoration, water storage, and nutrients, the financial costs, and a slow ecological process, due to the adverse circumstances found in the area. be restored. The strong anthropogenic interventions by mining processes reduce dramatically the physical and nutritional composition of the soil. We studied two restoration areas in Paracatu, Brazil, to examine their edaphic conditions six years after mining activities ceased and relate them to the status of the restoration process. In 2009, a Cerrado restoration were established in an area previously explored for gravel extraction. Plants were sampled and identified in 11 transects along the planting lines. The diameter base (DB) and total height (HT) were measured. The physical/chemical quality of the soil substrate was determined using a collection of samples in open trenches at four types of points: Cerrado (TC); dead plant pits (TM); seedling pits having living individuals of the most abundant species (TT); and those of the second-most abundant species (TE). Cecropia pachystachya Trécul and Tapirira guianensis Aubl. were most abundant and demonstrated the potential to thrive in areas degraded by mining having low mortality rates and growth at relatively DB and HT. The physical quality indicators in the gravel pits were not limiting, indicating that substrate preparation was efficient in this regard. The organic matter content in TM, TT, and TE was low in comparison to that of TC, and the chemical conditions in the TE pit substrates were similar to those in TM pits, suggesting C. pachystachya is a species with good plasticity, whereas T. guianensis is present in pits with higher levels of phosphorus.

Therapeutic Potential of Human Immature Dental Pulp Stem Cells Observed in Mouse Model for Acquired Aplastic Anemia.

Aplastic anemia (AA) is a rare and serious disorder of hematopoietic stem cells (HSCs) that results in the loss of blood cells due to the failure of the bone marrow (BM). Although BM transplantation is used to treat AA, its use is limited by donor availability. In this sense, mesenchymal stem cells (MSCs) can offer a novel therapeutic approach for AA. This is because the MSCs contribute to the hematopoietic niche organization through their repopulating. In our study, we used the human immature dental pulp stem cell (hIDPSC), an MSC-like cell, to explore an alternative therapeutic approach for AA. For this, isogenic C57BL/6 mice were exposed to total body irradiation (TBI) to induce the AA. After 48 h of TBI, the mice were intraperitoneally treated with hIDPSC. The immunohistochemistry analyses confirmed that the hIDPSCs migrated and grafted in the mouse bone marrow (BM) and spleen, providing rapid support to hematopoiesis recovery compared to the group exposed to radiation, but not to those treated with the cells as well as the hematological parameters. Six months after the last hIDPSC transplantation, the BM showed long-term stable hematopoiesis. Our data highlight the therapeutic plasticity and hematoprotective role of hIDPSC for AA and potentially for other hematopoietic failures.

Synthesis, In Vitro Testing, and Biodistribution of Surfactant-Free Radioactive Nanoparticles for Cancer Treatment.

New forms of cancer treatment, which are effective, have simple manufacturing processes, and easily transportable, are of the utmost necessity. In this work, a methodology for the synthesis of radioactive Gold-198 nanoparticles without the use of surfactants was described. The nuclear activated Gold-198 foils were transformed into H198AuCl4 by dissolution using aqua regia, following a set of steps in a specially designed leak-tight setup. Gold-198 nanoparticles were synthesized using a citrate reduction stabilized with PEG. In addition, TEM results for the non-radioactive product presented an average size of 11.0 nm. The DLS and results for the radioactive 198AuNPs presented an average size of 8.7 nm. Moreover, the DLS results for the PEG-198AuNPs presented a 32.6 nm average size. Cell line tests showed no cytotoxic effect in any period and the concentrations were evaluated. Furthermore, in vivo testing showed a high biological uptake in the tumor and a cancer growth arrest.

Uncovering Novel Plasma Membrane Carboxylate Transporters in the Yeast Cyberlindnera jadinii.

The yeast Cyberlindnera jadinii has great potential in the biotechnology industry due to its ability to produce a variety of compounds of interest, including carboxylic acids. In this work, we identified genes encoding carboxylate transporters from this yeast species. The functional characterization of sixteen plasma membrane carboxylate transporters belonging to the AceTr, SHS, TDT, MCT, SSS, and DASS families was performed by heterologous expression in Saccharomyces cerevisiae. The newly identified C. jadinii transporters present specificity for mono-, di-, and tricarboxylates. The transporters CjAto5, CjJen6, CjSlc5, and CjSlc13-1 display the broadest substrate specificity; CjAto2 accepts mono- and dicarboxylates; and CjAto1,3,4, CjJen1-5, CjSlc16, and CjSlc13-2 are specific for monocarboxylic acids. A detailed characterization of these transporters, including phylogenetic reconstruction, 3D structure prediction, and molecular docking analysis is presented here. The properties presented by these transporters make them interesting targets to be explored as organic acid exporters in microbial cell factories.

A classification of cultivated pastures in the Brazilian Cerrado for sustainable intensification and savanna restoration.

The Brazilian Cerrado, with over 200 million hectares, has approximately 28% of its area occupied by cultivated pasturelands and 39% of them are degraded. In this study, we propose a new classification of the Cerrado pastures and recommendations for sustainable intensification and savanna restoration. We propose seven classes of pastures based on the ground cover proportions of exotic grass, bare soil, and native vegetation. These lands need to be acknowledged for their biodiversity conservation and potential for sustainable intensification and restoration. In order to make ecological intensification available for the ranchers, research and technology transfer have to embrace native tree species-based silviculture, native-grass-based forage management and enhancement, and value chain of biodiversity-friendly products. The pasture management proposals of this paper are based on a concept of biodiversity as an ecosystem service, promoting local productivity and global ecosystem services.