RESUMO
Polyelectrolytic multilayers (PEMs) with enhanced antibacterial properties were built up onto commercial poly(ethylene terephthalate) (PET) films based on the layer by layer assembling of bacterial contact killing chitosan and bacterial repelling highly hydrated hyaluronic acid. The optimization of the aminolysis modification reaction of PET was carried out by the study of the mechanical properties and the surface characterization of the modified polymers. The layer by layer assembly was successfully monitored by TEM microscopy, surface zeta-potential, contact angle measurements and, after labeling with fluorescein isothiocyanate (FTIC) by absorption spectroscopy and confocal fluorescent microscopy. Beside, the stability of the PEMs was studied at physiological conditions in absence and in the presence of lysozyme and hyaluronidase enzymes. Antibacterial properties of the obtained PEMs against Escherichia coli were compared with original commercial PET.
Assuntos
Antibacterianos/química , Quitosana/análogos & derivados , Quitosana/química , Materiais Revestidos Biocompatíveis/química , Ácido Hialurônico/análogos & derivados , Ácido Hialurônico/química , Polietilenotereftalatos/química , Antibacterianos/farmacologia , Quitosana/farmacologia , Materiais Revestidos Biocompatíveis/farmacologia , Escherichia coli/efeitos dos fármacos , Fluoresceína-5-Isotiocianato/química , Ácido Hialurônico/farmacologia , Hialuronoglucosaminidase/química , Muramidase/química , Polietilenotereftalatos/farmacologiaRESUMO
This study aims to synthesize, characterize and investigate the water solubility and cytotoxicity of branched anionic/cationic ß-cyclodextrins (bßCDs) obtained by reaction with epichlorohydrin and chloroacetic acid or choline chloride, respectively, by a single step polycondensation reaction. Obtained ionic bßCDs were investigated as an attempt to comparatively study anionic and cationic bßCDs. Water solubility of both ionic derivatives was similar (400 mg/mL) at neutral and basic pHs and remarkably higher than that of their neutral homologues. Additionally, a pH-dependent solubility of anionic bßCDs was observed. Cytotoxicity of ionic bßCDs was evaluated on Human colon carcinoma Caco-2 cells and high cell viability (>99%) was observed in the range of 0-100 mg/mL for anionic and cationic samples, in the same range of that of neutral and parent ß-CDs. Additionally, complexes formation capacity with triclosan, a poor water soluble antimicrobial agent, was confirmed by several techniques observing a complexation limit around 4 mg/mL for both systems and higher stability constant for anionic bßCDs than cationic derivatives.