[A comparative study of the level of plasma prolactin in women and men undergoing operations under halothane and neuroleptanesthesia]. / Sravnitelno prouchvane na nivoto na plazmeniia prolaktin pri zheni i muzhe pri operatsii, izvursheni pod khalotanova i nevroleptanestezia.

Twenty women and 20 men subjected to urologic operations comprise the study group. Ten women and 10 men were operated under halothane anesthesia and 10 women and 10 men--under neuroleptanesthesia. Plasma prolactin levels were determined before induction in anesthesia, and 30 and 60 minutes after the onset of the operation. The mean plasma prolactin level in women exceeded the upper normal limit at the first determination before induction in anesthesia. During the operation, both in women and in men, with either type of anesthesia there occurred statistically significant rise in the mean plasma prolactin, more pronounced in women than in men. In women, the rise in plasma prolactin was greater during operations performed under halothane anesthesia and in men--under neuroleptanesthesia.

[The differential diagnosis of hairy virilism in polycystic ovaries from adrenal cortical hirsutism]. / Diferentsialna diagnoza na kosmeniia virilizum pri polikistozni iaichnitsi ot nadbubrechnokorov khirzutizum.

The hormones on the axis hypophysis-ovary and hypophysis-suprarenal gland were examined in 60 women with polycystic ovaries and hairy virilism, 53 women with hirsutism and 45 healthy women, all of the same age. The results show that in hairy virilism from ovarian origin the luteinizing hormone level (LH) is increased 100% and the follicle stimulating hormone level (FSH) is decreased 200%. The index LH/FSH is increased more than 3 times. The testosterone and androstanedione levels are increased, too. In patients with suprarenal-cortex hirsutism the levels of ACTH, androstanedione, dehydroepiandrosterone-sulfate, testosterone and cortisol are increased. A hirsutism from running with hyperprolactinemia is differentiated. The three hirsutism forms may appear as separate forms or in combination.

Effect of diazepam and medazepam on prolactin secretion.

Pharmacological studies of the effect of Diazepam and Medazepam on prolactin secretion were carried out on sexually mature male albino rats. The experiments were carried out using two series of experimental set-ups. In the first series an analysis was made of the effect of Diazepam in doses of 2 and 4 mg/kg body mass, and of Medazepam in doses of 1 and 10 mg/kg body mass, on the prolactin secretion on the 60th min after a single intraperitoneal injection. The second series of experiments was designed to investigate the effect of Diazepam and Medazepam in the same doses under conditions of acute immobilization of the animals for 60 min. The prolactin levels in the serum were tested using a radioimmunological method. Prolactin secretion decreases after a single intraperitoneal administration of Diazepam in both doses. Prolactin content in the serum was reduced only after a dose of 10 mg/kg body mass. Under conditions of experimental immobilization stress, prolactin secretion was stimulated in the animals from the control group, treated with standard solution used as solvent. Diazepam in both doses administered and Medazepam in a dose of 10 mg/kg inhibit the prolactin secretion stimulated by acute immobilization stress.

Neuro-endocrine effects of isotheoline.

Experiments are carried out to study the effect of isotheoline (IST) on the secretion of prolactin (PRL), somatotrophic hormone (STH) and testosterone, using cats and Wistar albino rats of both sexes. PRL, STH and testosterone using cats and wistar albino rats of both sexes. PRL, STH and testosterone were determined radioimmunologically at the 10th, 60th and 120th min after the administration of IST in doses of 0.5 to 5 mg/kg. Comparative experiments were carried out with bromocryptine and glaucine--1 mg/kg i.p., as well as experiments involving the administration of IST on the background of the dopaminergic antagonists haloperidol and pymoside--1 mg/kg i.p. The results obtained show that in doses not exceeding 1 mg/ks IST increases the plasma level of testosterone and, similar to bromocryptine, reduces the plasma level of PRL and increases that of STH. These effects are less pronounced for the 5 mg/kg dose and they are antagonized by the dopaminergic antagonists used. It is assumed that IST, similar to bromocryptine, achieves the described effects through selective stimulation of the D2 dopaminergic receptors involved in neuro-endocrine mechanisms responsible for the control and release of the hormones studied.

[Plasma levels of follicle-stimulating and luteinizing hormones, prolactin and estradiol in women with chronic kidney failure on programmed hemodialysis]. / Plazemni niva na folikulostimulirashtiia (FSKh) i luteinizirashtiia (LKh) khormon, prolaktin (P) i estradiol (E) pri zheni s khronichna bubrechna nedostatuchnost (KhBN) na programma khemodializa.

The plasma level of FSH, LH, P and E of 24 women with CRI, aged from 16-61, on a programmed hemodialysis was determined, 13 of them with Balkan endemic nephropathy, 8--chronic pyelonephritis and one patient with chronic glomerulonephritis, polycystosis of kidneys and lupus nephritis. The patients were grouped into three groups according to age and genital cycle: with genital cycle--10, secondary amenorrhea--7, and in menopause--7. The patients with genital cycle were grouped as follows: 4 with eumenorrea, 4--opsomenorrhea and I with hyper- and I with hypomenorrhea. Plasma hormone level prior to and post hemodialysis was determined by radioimmunologic methods. The results obtained showed that FSH levels, as compared with the control group of healthy women, was unchanged, LH and P--was elevated and E was elevated only in the patients with genital cycle. No significant discrepancy was found in the levels of the hormones studied, in the three groups of females with CRI on programmed hemodialysis (with genital cycle, secondary amenorrhea and menopause), except for E in the women with cycle. The studies of the authors showed that plasma levels of FSH, LH, P and E prior to and post hemodialysis, are insignificantly changed. That provided grounds to admit that the hormones are nondialysable. The authors admit that the programmed hemodialysis is a treatment method by which the genital cycle in some CRI patients with secondary amenorrhea could be restored.

[Thyrotropic hormone plasma level in thyrotoxicosis]. / Plazmeno nivo na tireotropniia khormon pri bolni s tireotoksikoza

Immunoreactive TTH plasma concentration was investigated twice a day (at 9 a.m. and 9 p.m.) in 30 patients with thyrotoxicosis, 10 out of them in acute stage and 20 in a state of remission after thymidazol treatment as well as in 17 healthy subjects. The authors established that in the acute state of thyrotoxicosis the latter is reduced to low normal or subnormal values (an average of 2.94 +/- 0.70 ng/ml) and after thyrostatic therapy leading to remission--is about the average norm (4.65 +/- 1.10 ng/ml) and does not differ much from that in healthy subjects (4.75 +/- 0.92 ng/ml). The negative correlation between the TIH levels and thyroid hormones (BOI, total thyroxin), typical for the healthy subjects, is found in the patients with acute thyrotoxicosis. No significant difference was established between the TTH values of the morning and evening hours in any of the groups. The physiological regulatory feedback mechanism in thyrotoxicosis is assumed not to be disturbed.