Expression of eukaryotic-like protein in the microbiome of sponges.

Eukaryotic-like proteins (ELPs) are classes of proteins that are found in prokaryotes, but have a likely evolutionary origin in eukaryotes. ELPs have been postulated to mediate host-microbiome interactions. Recent work has discovered that prokaryotic symbionts of sponges contain abundant and diverse genes for ELPs, which could modulate interactions with their filter-feeding and phagocytic host. However, the extent to which these ELP genes are actually used and expressed by the symbionts is poorly understood. Here, we use metatranscriptomics to investigate ELP expression in the microbiomes of three different sponges (Cymbastella concentrica, Scopalina sp. and Tedania anhelens). We developed a workflow with optimized rRNA removal and in silico subtraction of host sequences to obtain a reliable symbiont metatranscriptome. This showed that between 1.3% and 2.3% of all symbiont transcripts contain genes for ELPs. Two classes of ELPs (cadherin and tetratricopeptide repeats) were abundantly expressed in the C. concentrica and Scopalina sp. microbiomes, while ankyrin repeat ELPs were predominant in the T. anhelens metatranscriptome. Comparison with transcripts that do not encode ELPs indicated a constitutive expression of ELPs across a range of bacterial and archaeal symbionts. Expressed ELPs also contained domains involved in protein secretion and/or were co-expressed with proteins involved in extracellular transport. This suggests these ELPs are likely exported, which could allow for direct interaction with the sponge. Our study shows that ELP genes in sponge symbionts represent actively expressed functions that could mediate molecular interaction between symbiosis partners.

Non-institutionalized nonagenarians health-related quality of life and nutritional status: is there a link between them?

INTRODUCTION: Nonagenarian population, clearly increasing, shows different characteristics from the rest of elderly people. Health-related quality of life is a way to study population health in physical, psychological and social dimensions. OBJECTIVES: To examine the relationship between nutritional status and health-related quality of life in a group of free-living nonagenarians. Differences with octogenarians were also studied. METHODS: Within Villanueva Older Health Study, 20 non-institutionalised people (92.5 ± 3.5 years; 80% women) make the nonagenarian subsample. Nutritional risk was assessed by Mininutritional Assessment questionnaire, dietary intake by a 24-hour dietary recall and health-related quality of life by EuroQoL-5D questionnaire. SPSS was used for statistical analysis. RESULTS: 40% nonagenarians were at risk of malnutrition. Dietary assessment showed magnesium, zinc, potassium, folic acid, vitamin D and vitamin E deficiencies. Problems in mobility were more frequently reported (80%). EQ-5Dindex was associated with MNA(p<0.05). Self-care dimension was associated with calcium and niacin(p<0.05), retinol and cholesterol(p<0.01) intake. Usual activities dimension was associated with niacin(p<0.01) and cholesterol(p<0.05) intake. Pain/discomfort dimension was associated with protein(p<0.01), energy, selenium and niacin(p<0.05) intake. Anxiety/depression was associated with protein(p<0.01) and selenium(p<0.05) intake. CONCLUSIONS: Risk of malnutrition is a factor associated to health-related quality of life. Results suggest that energy and some nutrient intakes could be possibly associated to health-related quality of life but further research on this influence is required.

Introducción: La población nonagenaria, en claro crecimiento, muestra características diferentes del resto de la población anciana. La calidad de vida relacionada con la salud es una forma de estudiar la salud de la población en sus dimensiones física, psicológica y social. Objetivos: Observar la relación entre el estado nutricional y la calidad de vida relacionada con la salud en un grupo de nonagenarios de vida independiente. También se estudian las diferencias con los octogenarios. Método: Dentro del Estudio sobre Salud en Mayores de Villanueva de la Cañada, 20 personas (92,5±3,5 años; 80% mujeres) forman la submuestra de nonagenarios. El riesgo nutricional fue valorado mediante el Mininutritional Assessment, la ingesta dietética mediante un recuerdo de 24 horas y la calidad de vida relacionada con la salud usando el cuestionario EuroQoL-5D (EQ-5D). Para el análisis estadístico se utilizó el programa SPSS. Resultados: 40% de los nonagenarios presentaban riesgo de malnutrición. Se detectaron posibles deficiencias de magnesio, cinc, potasio, ácido fólico, vitamina D y vitamina E. Los problemas de movilidad fueron los más frecuentes (80%). El EQ-5Dindice se asoció con el MNA(p.

Influence of nutritional status on health-related quality of life of non-institutionalized older people.

OBJECTIVES: Health-related quality of life (HRQoL) is a multidimensional health measurement and a key to optimal aging. The aim of this study was to examine the association of nutritional status with HRQoL in the elderly. DESIGN: Cross-sectional study. SETTING: Villanueva Older Health Study, a community-based study in Villanueva de la Cañada (Madrid, Spain). PARTICIPANTS: 83 (53 women) non-institutionalized inhabitants aged 80 years and above. MEASUREMENT: HRQoL was assessed by EuroQoL-5D (EQ-5D) questionnaire, nutritional risk by Mini Nutritional Assessment (MNA) questionnaire and dietary intake by 24-hour dietary recall. Statistical significance was evaluated at 95% confidence level (P<0.05). RESULTS: EQ-5D pointed out differences between men and women (0.782±0.235 and 0.633±0.247; p=0.02). Problems in mobility (total sample) and pain/discomfort (women) dimensions were most frequently reported. MNA (26.5±3.2 men and 24.3±3.2 women; p=0.03) revealed malnutrition in 3.3% of men and 1.9% of women, and risk of malnutrition in 6.7% and 37.7%, respectively. Total sample was at risk of folic acid, zinc, magnesium, vitamin D and vitamin E deficiency. EQ-5D was associated with MNA (p<0.001). EQ-5Dindex was associated with energy intake (p=0.04) and EQ-5Dvas was negatively correlated with body mass index (p=0.02). EQ-5D pain/discomfort dimension was associated with energy (p=0.006), protein (p=0.005), lipid (p=0.03), magnesium (p=0.032), phosphorus (p=0.012), selenium (p=0.043) and niacin (p=0.004) intake. CONCLUSIONS: Women showed poorer HRQoL and higher malnutrition risk. A relationship between HRQoL and risk of malnutrition was observed. Results suggest that when energy and protein, lipid, phosphorus, magnesium, selenium and niacin intake increase, HRQoL is promoted, although the increase does not seem to have a strong direct effect on it. The limited influence of energy and nutrient intake on HRQoL observed requires further research.

Specificity and temporal dynamics of complex bacteria--sponge symbiotic interactions.

Microbes are known to form intricate and intimate relationships with most animal and plant taxa. Microbe--host symbiotic associations are poorly explored in comparison with other species interaction networks. The current paradigm on symbiosis research stems from species-poor systems where pairwise and reciprocally specialized interactions between a single microbe and a single host that coevolve are the norm. These symbioses involving just a few species are fascinating in their own right, but more diverse and complex host-associated microbial communities are increasingly found, with new emerging questions that require new paradigms and approaches. Here we adopt an intermediate complexity approach to study the specificity, phylogenetic community structure, and temporal variability of the subset of the most abundant bacteria associated with different sponge host species with diverse eco-evolutionary characteristics. We do so by using a monthly resolved annual temporal series of host-associated and free-living bacteria. Bacteria are very abundant and diverse within marine sponges, and these symbiotic interactions are hypothesized to have a very ancient origin. We show that host-bacteria reciprocal specialization depends on the temporal scale and level of taxonomic aggregation considered. Sponge hosts with similar eco-evolutionary characteristics (e.g., volume of tissue corresponding to microbes, water filtering rates, and microbial transmission type) have similar bacterial phylogenetic community structure when looking at interactions aggregated over time. In general, sponge hosts hypothesized to form more intricate relationships with bacteria show a remarkably persistent bacterial community over time. Other hosts, however, show a large turnover similar to that observed for free-living bacterioplankton. Our study highlights the importance of exploring temporal variability in host--microbe interaction networks if we aim to determine how specific and persistent these poorly explored but extremely common interactions are.

[Comparative analysis of vitamin D status and solar exposition habits in adolescent and elderly Spanish women. The Five Countries Study (OPTIFORD Project)]. / Análisis comparativo del estado nutricional de vitamina D y de los hábitos de exposición solar de las participantes Españolas (adolescentes y de edad avanzada) del Estudio de los Cinco Países (Proyecto OPTIFORD).

INTRODUCTION: Vitamin D deficiency is known to be very common in adolescent girls and elderly women. AIM: To analyze vitamin D status, vitamin D intake and solar exposure of Spanish participants in The Five Countries Study of Optiford Project. METHODS: Questionnaires approved and validated of lifestyle and food consumption frequency applied. The biochemical analysis of 25-hidroxivitamin D and PTH and the evaluation of the solar exposure (dosymeter UV VioSpor) were carried out. RESULTS: The average solar exposure of adolescent girls (1,519 J/m²) was double than elderly women (740 J/m²). The vitamin D status of adolescent girls in summer and winter was better than elderly women. CONCLUSIONS: Solar exposure habits are different between the two age groups. This fact, jointly to the lower body's capacity to synthesize Vitamin D through exposure to the sun in the elderly, may justify the worse vitamin D status in this age group.

[Influence of sun exposure and diet to the nutritional status of vitamin D in adolescent Spanish women: the five countries study (OPTIFORD Project)]. / Influencia de la exposición solar y la dieta en el estado nutricional de vitamina D en adolescentes españolas: estudio de los cinco países (Proyecto OPTIFORD).

INTRODUCTION: Vitamin D deficiency is a big public health concern, especially among women. For this reason, "The Five Countries Study" was set up within the OPTIFORD project. This study aims at knowing the vitamin D status in adolescent and older women in five European countries according to their different dietary and behavioral habits. OBJECTIVES: To analyze the relative contribution of sun exposure and the diet to the vitamin D status among 47 Spanish adolescents participating in "The Five Countries Study". METHODS: Validated and standardized questionnaires (health, lifestyle, sun exposure, and frequency of food consumption), biochemical analysis (25-hydroxyvitamin D (S-25-OHD) and parathormone), and assessment of sun exposure (UV dosimeter). RESULTS: Serum levels of S-25-OHD were higher during the summertime than in winter (61.55±12.85 nmol/l and 45.81±9.29 nmol/l, respectively). Forty-five percent of the sample had a worsening of their vitamin status in the winter, as compared with the summer (p<0.0001). During the summer, vitamin D insufficiency (S-25-OHD≤50 nmol/l) affected 17% of the population, going up to 63% in the winter. Sixty-six percent of the sample did not reach the IR of vitamin D (5 g/day), being the fish the main dietary source of vitamin D. CONCLUSIONS: Only 34% of the participants had an adequate vitamin D status. Both adequate sun exposure and vitamin D dietary intake are recommended in order to correct deficient and insufficient situations.

[The contribution of diet and sun exposure to the nutritional status of vitamin D in elderly Spanish women: the five countries study (OPTIFORD Project)]. / Contribución de la dieta y la exposición solar al estatus nutricional de vitamina D en españolas de edad avanzada: Estudio de los Cinco Países (Proyecto OPTIFORD).

UNLABELLED: Vitamin D deficiency represents an important public health problem, especially among elderly people, by increasing the morbimortality. Because of the importance of this, in the year 2001 the "Five Countries Study" was put in place, within the European OPTIFORD project (Towards a strategy for optimal vitamin D fortification). This cross-sectional and observational study aims at knowing the vitamin D status in adolescent and elder women from five European countries, including Spain, according to different dietary and behavioral habits. OBJECTIVE: This work analyzes the relative contribution of sun exposure and diet to the vitamin D status in 53 Caucasian Spanish elder women (72 +/- 1.6 years), with an autonomous life, participating at the Five Countries Study. METHODOLOGY: The information was gathered in summer and winter time, by using homologated and validated questionnaires: health status and life style questionnaire; assessment of sun exposure by a standardized test and using a Viospor UV dosimeter (only in summer time); biochemical analysis of 25 hydroxyvitamin D (S-25-OHD) and parathyroid hormone; and questionnaire of frequency of selective intake. RESULTS: We observed a direct relationship between sun exposure measured with the dosimeter (741 +/- 624 J/m2) and the number of hours outdoors during the measuring week (3.4 +/- 1.9 hours/day) (p < 0.0001). The serum levels of S-25-OHD were higher in summer time than during the winter (40.32 +/- 20.39 nmol/L and 30.08 +/- 17.39 nmol/L, respectively), and 40% of the participants had worse vitamin status in winter as compared with summer time (p < 0.001). During the summer time, vitamin D deficiency (S-25-OHD < or = 25 nmol/L) affected 28% of the population, being virtually twice as much during the winter time. The higher the sun exposure, as assessed by the dosimeter, the higher the value of S-25-OHD, with clear differences between participants in the S-25-OHD < or = 25 nmol/L group and those in the S-25-OHD > 50 nmol/L (p = 0.01). There is also a direct association between the number of hours outdoors and S-25-OHD (p = 0.09), with differences between the participants in the S-25-OHD < or = 25 nmol/L group and the S-25-OHD > 50 nmol/L group. In more than 95% of the sample, mean dietary intakes of vitamin D (5.17 +/- 4.84 microg/day in summer time and 4.70+/- 4.72 microg/day in winter time), the main source of which being fish, did not cover the Recommended Allowances. We did not observe a relationship between the dietary intake and blood levels of vitamin D. By contrast, those participants taking vitamin D supplements presented higher S-25-OHD levels (summer = 69.64 nmol/L and winter = 55 nmol/L) than those not consuming it (summer = 36.83 nmol/L and winter = 25.82 nmol/L) (psummer =0.0003 and p winter < 0.001). CONCLUSIONS: The deficient status of vitamin D among the elderly female population has to be corrected, whenever possible, with appropriate sun exposure and an increase in vitamin D intake through the diet, assessing at each particular case the benefits of pharmacological supplementation.

Greenhouse gas emission reduction and environmental quality improvement from implementation of aerobic waste treatment systems in swine farms.

Trading of greenhouse gas (GHG) emission reductions is an attractive approach to help producers implement cleaner treatment technologies to replace current anaerobic lagoons. Our objectives were to estimate greenhouse gas (GHG) emission reductions from implementation of aerobic technology in USA swine farms. Emission reductions were calculated using the approved United Nations framework convention on climate change (UNFCCC) methodology in conjunction with monitoring information collected during full-scale demonstration of the new treatment system in a 4360-head swine operation in North Carolina (USA). Emission sources for the project and baseline manure management system were methane (CH4) emissions from the decomposition of manure under anaerobic conditions and nitrous oxide (N2O) emissions during storage and handling of manure in the manure management system. Emission reductions resulted from the difference between total project and baseline emissions. The project activity included an on-farm wastewater treatment system consisting of liquid-solid separation, treatment of the separated liquid using aerobic biological N removal, chemical disinfection and soluble P removal using lime. The project activity was completed with a centralized facility that used aerobic composting to process the separated solids. Replacement of the lagoon technology with the cleaner aerobic technology reduced GHG emissions 96.9%, from 4972 tonnes of carbon dioxide equivalents (CO2-eq) to 153 tonnes CO2-eq/year. Total net emission reductions by the project activity in the 4360-head finishing operation were 4776.6 tonnes CO2-eq per year or 1.10 tonnes CO2-eq/head per year. The dollar value from implementation of this project in this swine farm was US$19,106/year using current Chicago Climate Exchange trading values of US$4/t CO2. This translates into a direct economic benefit to the producer of US$1.75 per finished pig. Thus, GHG emission reductions and credits can help compensate for the higher installation cost of cleaner aerobic technologies and facilitate producer adoption of environmentally superior technologies to replace current anaerobic lagoons in the USA.

[Intimate partner violence in Spain]. / Violencia del compañero íntimo en España.

OBJECTIVE: To estimate mortality and the incidence of formal complaints of intimate partner violence (IPV) by age groups and Spanish provinces. METHODS: We performed a descriptive, epidemiological study of murders by intimate partners registered in the database of the Divorced and Separated Women's Federation (1998-2001) and of formal complaints of IPV in The Home Affairs database (1997-2000). The percentage of increase in formal complaints and deaths and the individual risk of dying from IPV and of reporting this crime was calculated through the simple accumulative method, specific mortality rates by age, average rates by provinces, and specific incidence rates of formal complaints of IPV by provinces. Finally, a simple regression analysis was performed to assess the relation between mortality and the incidence of formal complaints of IPV. RESULTS: Between the first and the last year of the study, formal complaints increased by 27% and murders increased by 49%. In Spain, during the period analyzed, one out of every 100,000 women was murdered by an intimate partner and one out of every 200 women reported IPV. Women aged 22-41 years and those aged 82-86 years presented the highest rates of mortality from IPV. Mortality from IPV and the incidence of formal complaints of IPV showed no clear geographical pattern. No correlation between mortality and the incidence of formal complaints of IPV by provinces was found (corrected r2 = 0.016). CONCLUSIONS: IPV is an increasing public health problem in Spain. There seems to be a bimodal curve by age in mortality from IPV, but no geographical patterns in mortality and the incidence of formal complaints of IPV or between the provinces with the highest incidence of formal complains and those with highest mortality were observed.

Nutritional regulation of glucose-6-phosphatase gene expression in liver of the gilthead sea bream (Sparus aurata).

To examine the role of glucose-6-phosphatase (G6Pase) in glucose homeostasis in the diabetes-like experimental model of carnivorous fish, we analysed postprandial variations and the effect of starvation, ration size and diet composition on the regulation of G6Pase expression at the enzyme activity and mRNA level in the liver of gilthead sea bream (Sparus aurata). G6Pase expression increased in long-term starved or energy-restricted fish. In contrast to data reported for other fish species, short-term regulation of G6Pase expression was found in regularly fed S. aurata. G6Pase mRNA levels were lowest between 4 and 15 h after food intake, whereas minimal enzyme activity was observed 10-15 h postprandially. Alterations of plasma glucose levels affect G6Pase in mammals. However, the carbohydrate content of the diet did not affect hepatic expression of G6Pase in S. aurata, suggesting that a different molecular mechanism is involved in the control of G6Pase expression in fish. Although G6Pase was unaffected, high-carbohydrate low-protein diets increased glucokinase (GK) expression and thus allowed a metabolic adaptation favouring glycolysis over gluconeogenesis. Interestingly, only the nutritional conditions that promoted variations in the blood glucose levels resulted in changes in the hepatic expression of G6Pase. These findings indicate a concerted regulation of G6Pase and GK expression and suggest that the direction and rate of the glucose-glucose-6-phosphate substrate cycle flux is finely regulated in the liver of S. aurata, challenging the role attributed to deficient regulation of G6Pase or GK expression in the low ability of carnivorous fish to metabolize glucose.

Coordinated modulation of the fibroblast growth factor dual receptor mechanism during transformation from human colon adenoma to carcinoma.

Basic fibroblast growth factor (bFGF) is dependent on heparan sulphate for its ability to activate the cell surface signal transducing receptor. We have investigated the FGF dual receptor mechanism in a novel model of the transformation from human colon adenoma to carcinoma in vitro. Reverse transcription-polymerase chain reaction showed that mRNA for FGF receptors 1 and 2 were expressed in both the adenoma and carcinoma cells whereas immunocytochemistry showed that the expression of the FGF R1 was reduced significantly in the carcinoma cells. We have reported previously that the composition and sequence of human colon adenoma and carcinoma heparan sulphate (HS) differ in a defined and specific manner. The functional significance of these changes was assessed by affinity co-electrophoresis, which showed that the affinity of adenoma HS for bFGF was 10-fold greater than that of the carcinoma HS (Kd 220 nM vs. 2493 nM, respectively). In addition, Northern studies of the expression of syndecan 1 and 4 mRNA showed that proteoglycan core protein expression was reduced significantly in the carcinoma cells. These findings were associated with a reduced biological response to bFGF in the carcinoma cells that could be partially reversed by the addition of exogenous heparin, suggesting that both the proteoglycan and signal transducing receptor control the cells' response to bFGF.

Molecular spectra of HPRT deletion mutations in circulating T-lymphocytes in Fanconi anemia patients.

The principal cellular feature of Fanconi anemia (FA), an inherited cancer prone disorder, is a high level of chromosomal breakage, amplified after treatment with crosslinking agents. Three of the eight genes involved in FA have been cloned: FANCA, FANCC and FANCG. However, their biological functions remain unknown. We previously observed an excessive production of deletions at the HPRT locus in FA lymphoblasts belonging to the relatively rare complementation group D(1) and an increased frequency of glycophorin A (GPA) variants in erythrocytes derived from FA patients (2). In thi study, we examined the molecular nature of 31 HPRT mutations formed in vivo in circulating T-lymphocytes isolated from 9 FA male patients. The results show that in all FA patients investigated the deletions are by far the most prevalent mutational event in contrast to age matched healthy donors, in which point mutations predominate. The complementation group in the FA patients examined in the present study has not yet been defined. However, knowing that mutations in the FANCA and FANCC gene are found to be involved in at least 70% of the FA patients, it can be expected that the excessive production of deletions is a general feature of the FA phenotype. In addition, the spectrum of HPRT deletions observed in FA patients differs from that of healthy children: there is a high frequency of 3'-terminal deletions and a strikingly low proportion of V(D)J mediated events. Based on previous findings, a decreased fidelity of coding V(D)J joint formation (3) and an inaccurate repair of specific DNA double strand breaks via Non-Homologous End Joining (4), we propose that FA genes play a role in the control of the fidelity of rejoining of specific DNA ends. Such a defect may explain several basic features of FA, such as chromosomal instability and deletion pronenness.

[Evaluation of measurements intended to develop regularity of blood donation]. / Evaluation de mesures destinées à développer la régularité du don de sang.

An increase in the regularity of blood donation is desirable for two main reasons. First, the lower incidence of viral disease markers in regular donors when compared to first donors could help to reduce risks of transmissible diseases. Second, a higher frequency of donation could contribute to a more satisfactory supply of blood products. Two measures implemented with the aim of increasing the regularity of blood donation were evaluated: (i) an increase in the annual frequency of blood collection by mobile teams at collection sites, and (ii) a "next donation document" given to each donor indicating the earliest possible date of the next donation. The regularity index was calculated as the mean number of cellular donations (whole blood and platelet apheresis) per donor per year, over two consecutive periods: 1-7-1993-30-6-1994 (P1) and 1-7-1994-30-6-1995 (P2). The junction of these two periods corresponded to the date of introduction of the "next donation document" and to the time of reinforcement of the mobile collection frequency. First donors in either period were not taken into account in the study. A significant relationship was observed between the annual frequency of mobile collection and the average number of donations per donor (comparison test of two means on large samples, p < 0.001 in all the cases excepted those of stable mobile collection numbers). Thus, in the first period, the average donation rate was the lowest on sites having only one mobile collection per year (M: 1.55, W: 1.38) and the highest on sites with five mobile collections per year (M: 2.05, W: 1.71). These average numbers significantly increased in the second period as compared to the first (M: +4.8%, W: +1.8%; comparison test of two means from paired series, p < 0.001), as did the yearly frequency of mobile blood collections (+9.2%). On the other hand, the "next donation document" was not associated to any change in the regularity index. The results of this study therefore showed an increase in the frequency of visits of mobile collection teams to be the main factor leading to an improvement in the regularity of blood donation. Moreover, this evolution was associated with a decrease in the incidence of viral markers detected at donation.

[Staff certification for mobile blood collection units]. / Une expérience de certification du personnel des prélèvements en collectes mobiles.

Training and official acknowledgment of the competence of each staff member are essential to the quality and safety of collected blood products prepared and delivered by a blood transfusion center. A procedure was created to indicate in detail the methods employed to implement such accreditation. Based on individual training according to activity, it defines for each type of activity (secretary, physician, collector, driver) the required theoretical and practical knowledge of his/her position. Accreditation, consisting of assessment of the degree of competence attained in these areas of responsibility, was applied to the members of mobile blood collection teams in 1995. No major deficiency was detected, and this certification was well accepted by the staff. In order to complete this initial accreditation, blood collection abnormalities (inadequate blood volumes, clots or defective welding of tubing) were assessed for each collector individually. Comparison of these abnormalities in qualified nurses and laboratory technicians with a blood collection diploma showed no differences. On the other hand, significantly higher numbers of abnormalities were found in intermittent as compared to regular collectors and in senior as compared to new collectors. The applied corrective measures led to obviation of differences and improvement in performance. In 1996, in the first individual evaluation of medical selection carried out by each physician, discrepancies of one to 20 donors (0.7-14.2%) were observed from one doctor to another in the frequency of elimination of candidates for blood donation after the medical interview. Regular meetings with physicians resulted in reducing these discrepancies to one to 3.1 donors (4.6-14.1%) in 1997. In conclusion, the association of an initial accreditation procedure with an individual follow-up of work quality allowed satisfactory assessment of the training and competence of staff members. This kind of method could be extended to those working in other fields of transfusion medicine.

[Quality control of homologous blood collection activities: 5 years' experience]. / Contrôle de qualité des activités de prélévements homologues: 5 ans d'expérience.

Quality control (QC) of blood collection activities for transfusion is a regulatory requirement. The authors report on their experience in this field over the past 5 years. In their institution, this QC is based on both the recording and analyzing of predefined data, as well as the search for an active collaboration from each person involved in these activities. QC of medical selection relies on the assessment of several associated criteria: effectiveness of the information given to blood donors for recruitment, preparation of the medical interview and encouragement to perform regular donations; frequency of donors deferred after the medical interview; frequency of biological abnormalities detected at donation; results of the inquiries into the corresponding medical interviews following adverse transfusion reactions. The quantitative and qualitative evaluation of blood collection permits assessment of the quality of the blood collection program, collection procedures and directly derived blood products. Quality assessment of facilities and equipment is also included in this QC. Results have been improving in recent years, especially regarding medical selection. In particular, an increase in the mean donation rate of donors, a decrease in biological abnormalities detected at donation and an absence of adverse transfusion reactions attributed after inquiry to an inadequate medical interview have been noticed. A decrease in both shortage and outdating of labile blood products likewise indicates an improvement of blood collection planning. However, this QC reveals deficiencies in the information given to donors and a lack of analysis of the data specific to first time donors. In order to further improve the efficiency of QC, these results now require comparison with similar data collected on a nation-wide scale.

Overproduction and purification of an agarase of bacterial origin.

The agarase gene from Streptomyces coelicolor has been cloned in the non-producer bacterium Streptomyces lividans under the control of its own set of promoters and under the control of a heterologous promoter that is functional only during exponential growth. The best level of overproduction was obtained when the strain containing the natural gene was cultivated in fed batch with mannitol as carbon source. The protein, with a relative molecular mass of 32 kDa, has been purified following an affinity purification method. Contaminating activities seem to be absent from the purified enzyme preparation that can be used to purify DNA from agarose gels.

[Study of in vitro antimicrobial resistance of positive coprocultures to Shigella sp]. / Estudo da resistência antimicrobiana in vitro das coproculturas positivas para Shigella sp.

BACKGROUND: The gastroenteritis caused by Shigella sp (shigellosis) represents an important cause of morbimortality, especially in children under two years old. It is well known that the proper antimicrobial therapy can improve clinical state and diminish the disease dissemination. Increasing levels of resistance to drugs commonly used in the treatment of shigellosis have been described, and its sensibility has not been recently evaluated in our setting. OBJECTIVE: To evaluate the Shigella antimicrobial resistance in our setting. METHODS: A retrospective study was conducted using 106 coprocultures of patients, hospitalized or not, who have been assisted at HCPA and at HPV, during the period of 1994 to 1996, with the sensibility test done through the Kirby-Bauer technique of diffusion discs. RESULTS: Out of the 106 coprocultures studied, 35 (33%) came from HPV and 71 (67%), from HCPA. Considering the species, 58.8% were S. flexneri, 39.6%, S. sonnei, 0.9%, S. disenteriae and 0.9%, S. boydii. The resistance levels to usually indicated antibiotics were: ampicillin -57.5%, trimethropim-sulfamethoxazol -75.5%, and nalidixic acid -7.5%. CONCLUSIONS: These results show significant (p<0.001) in vitro antimicrobial resistance to ampicillin and trimethoprim-sulfamethoxazol and low resistance to nalidixic acid among Shigella sp. Thus, nalidixic acid may be an option to treat infections caused by resistant organisms.

[Intake of pollutants (heavy metals) via total diet at the national level as a whole and in autonomous communities: a new approach]. / Ingesta de contaminantes (metales pesados) vía dieta total del Conjunto Nacional y Comunidades Autónomas: nuevo enfoque.

As an answer to the request of information on the real intake of pollutants, both WHO and FAO recommend its study through the study of total diet. Following their criteria we have determined the contents of arsenic, cadmium, mercury and lead in the total mean spanish diet and in the seventeen Autonomous Regions, comparing them with the acceptable daily intakes (ADI). At the same time it is studied the contribution of the different groups of nutrients to the total pollutant charge. There is no detectable health risk due to the intake through the total diet of arsenic, mercury or lead. However the intake of cadmium from the total national reaches 85% of ADI, which is higher in Galicia (153%), Canary Islands (129%) and Cantabria (103%) and is very close in Murcia (97%), Asturias (92%) and the Balearic Islands (85%).