RESUMO
Contractility of ovarian (OP) and cervical parts (CP) of uterus under the condition of immune-mediated injury which was induced by immunization with bovine serum albumin (BSA) was investigated. It was shown that under the activation of energy-synthesizing function of mitochondria with Mexidol the frequency of reductions in both uterine parts decreased, the amplitude and contractility index in the OP and CP as well as the duration of the active state in CP increased. Mexidol under the condition of immunization with BSA leads to the decrease in amplitude in 2,6 time and contractility index in 2,2 time in OP and to the increase of them in CP. It was shown that contractility features of ovarian and cervical parts of uterine under the condition of BSA- induced immunization were caused by changes of mitochondria functional state and were associated with nitric oxide.
Assuntos
Antioxidantes/farmacologia , Óxido Nítrico/metabolismo , Picolinas/farmacologia , Salpingite/prevenção & controle , Cervicite Uterina/prevenção & controle , Contração Uterina/efeitos dos fármacos , Animais , Bovinos , Feminino , Camundongos , Camundongos Endogâmicos CBA , Mitocôndrias/efeitos dos fármacos , Mitocôndrias/metabolismo , Salpingite/induzido quimicamente , Salpingite/metabolismo , Salpingite/fisiopatologia , Soroalbumina Bovina , Cervicite Uterina/induzido quimicamente , Cervicite Uterina/metabolismo , Cervicite Uterina/fisiopatologia , Contração Uterina/metabolismo , Útero/efeitos dos fármacos , Útero/metabolismo , Útero/fisiopatologia , Valina/análogos & derivados , Valina/farmacologiaRESUMO
BACKGROUND: The present study was conducted to investigate the expression of TNF-alpha and its receptors (types I and II) in both oocytes with germinal vesicle and the first polar body in mice. METHODS: Oocytes with intact germinal vesicle were isolated from mouse ovaries and subjected to in vitro maturation to obtain oocytes forming the first polar body. A reverse transcription polymerase chain reaction (RT-PCR) was used to examine the expression of TNF-alpha and its receptors at mRNA level. RESULTS: mRNA TNF-alpha was expressed in single oocytes and its level was decreasing during transition from germinal vesicle to the first polar body stage. At the same time the expression of TNF-receptors was not observed in single oocyte. CONCLUSIONS: These data are the important link in understanding of the molecular mechanisms regulating oocyte maturation as well as follicle development.