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2.
Infect Control Hosp Epidemiol ; 40(7): 804-806, 2019 07.
Artigo em Inglês | MEDLINE | ID: mdl-31088580

RESUMO

Whole-genome sequencing (WGS) has yielded new insights into the transmission patterns of healthcare facility-onset Clostridioides difficile infection (HO-CDI). WGS results prompted a focused diagnostic stewardship program, which was associated with a significant and sustained decrease in HO-CDI at large, urban hospital.


Assuntos
Clostridioides difficile/genética , Clostridioides difficile/isolamento & purificação , Infecções por Clostridium/diagnóstico , Infecções por Clostridium/transmissão , Infecção Hospitalar/diagnóstico , Infecção Hospitalar/microbiologia , Infecção Hospitalar/transmissão , Genoma Bacteriano , Instalações de Saúde , Humanos , Ribotipagem , Sequenciamento Completo do Genoma
3.
J Clin Microbiol ; 48(8): 2953-4, 2010 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-20519465

RESUMO

High-level mupirocin resistance (H-Mu(r)) in S. aureus is associated with the mupA gene. The mupA Evigene test rapidly identifies this gene. This study assessed the performance of mupA Evigene compared to that of susceptibility disk testing. mupA Evigene detected H-Mu(r) in 6/179 S. aureus isolates, and the results were concordant with those of susceptibility disk testing.


Assuntos
Antibacterianos/farmacologia , Proteínas de Bactérias/genética , Farmacorresistência Bacteriana , Técnicas de Diagnóstico Molecular/métodos , Mupirocina/farmacologia , Proteínas Nucleares/genética , Kit de Reagentes para Diagnóstico , Staphylococcus aureus/efeitos dos fármacos , Humanos , Testes de Sensibilidade Microbiana/métodos , Sensibilidade e Especificidade
5.
Ther Clin Risk Manag ; 4(3): 637-40, 2008 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-18827860

RESUMO

Fluorescence in situ hybridization using peptide nucleic acid probes (PNA-FISH) differentiates Staphylococcus aureus from other Gram-positive-cocci in clusters (GPCC). 101/202 patients with GPCC+ blood cultures were randomly assigned to clinician-notification of PNA FISH results. Notification was associated with reduced mortality (8% vs.17%, p = 0.05), further antibiotic use (median -2.5 days, p = 0.01), and trended toward reduced hospital stay and charges.

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