OneView strategy aligns physicians with Carilion.

Carilion's OneView strategy will ultimately integrate and link our ambulatory activities to our members, patients and community. It is our technology level to lift Carilion, our physician partners and patients to a new level of communication, cooperation, service and quality health care. It will take several years to complete the project, but by the turn of the century we will have OneView of our ambulatory world.

Modified techniques for the isolation of Dermatophilus spp. from infected material.

Two improved techniques are described for the isolation of Dermatophilus spp. The first is suitable for samples of scab from lesions of the disease in animals, except for sheep affected with lumpy wool. The second is for the isolation of the organism from samples of lumpy wool in sheep. Both techniques are simple, rapid and reliable and are based on releasing the organism by pulverizing the scab and then by suppressing contaminants by including polymixin B sulphate in the medium. Excellent results are obtained with or without pre-incubation exposure to CO2.

Morphology of colony variants of the rough form of Dermatophilus spp.

A technique is described for the preparation of intact colonies of Dermatophilus and their study under the scanning electron microscope. Using this technique with 38 strains of the organism it is possible to describe for the first time the patterns of colony morphology and to study the relationship of hyphae, cocci and zoospores in situ. Colonies were found to possess both vegetative and aerial hyphae. The various patterns of mycelial activity and structure that were seen raised serious doubts about the previous concepts of the mode of propagation and thus the classification of Dermatophilus within the Actinomycetales.

Use of activated charcoal for the removal of patulin from cider.

Penicillium urticae (NRRL 2159A) was grown in culture broth containing 1 muCi of [1-14C-A1acetate to produce [14C]patulin. [14C]patulin was purified from the broth and added to apple cider. After the patulin concentration of the cider was adjusted to 30 mug/ml with unlabeled patulin, the cider was subjected to various charcoal treatments. [14C]patulin was completely removed by shaking the cider with 20 mg of activated charcoal per ml and by eluting the cider through a 40- to 60-mesh charcoal column. Activated charcola at 5 mg/ml reduced patulin in naturally contaminated cider to nondetectable levels.

Fungicide selective for basidiomycetes.

Concentrations of 2,3-dihydro-5-carboxanilido-6-methyl-1,4-oxathiin lower than 8 parts per million prevented mycelial growth of a number of Basidiomycetes. By contrast, mycelial growth of various other fungi-Phycomycetes, Ascomycetes, and Deuteromycetes-was 50 percent inhibited only by concentrations of 32 ppm or higher. Two exceptions to this pattern of selective fungitoxicity were found:an isolate of Rhizoctonia solani was not as sensitive as other Basidiomycetes, and the deuteromycete Verticillium alboatrum was inhibited by lower concentrations than affected other fungi in this group. Spore germination of two Basidiomycetes, Uromyces phaseoli and Ustilago nuda, was inhibited 95 percent or more at 10 ppm.