RESUMO
Hepatitis and hepatocellular carcinoma are serious human diseases. Here, we examined the in vivo and in vitro inhibitory effect of extracts of Qizhu decoction (a traditional Chinese medicine) on hepatitis caused by diethylnitrosamine or hepatitis B virus and on diethylnitrosamine-induced hepatocellular carcinoma. The results showed that both the aqueous and ethanol extracts (QC and QS, respectively) of Qizhu decoction significantly inhibited hepatic inflammation and liver cancer induced by diethylnitrosamine or hepatitis B virus by suppressing NF-κB signaling and decreasing the levels of TNF-α and IL-1ß. Both QC and QS inhibited the proliferation and migration of primary cancer hepatocytes by reducing cyclin B1, cyclin D1 and N-cadherin expression and increasing E-cadherin expression. QC and QS also promoted the apoptosis of primary cancer hepatocytes by upregulating caspase-3 and downregulating BCL-2 expression. The knockdown of p65 in NF-κB signaling inhibited the ability of QC and QS to significantly reduce the colony formation ability of liver cancer cells. Additionally, QC and QS might significantly inhibit the DNA replication of hepatitis B virus in vivo and in vitro, and we found that corilagin and polydatin were the active compounds of QC and QS. Taken together, our in vitro findings and our results in C57BL/6 mice showed that extracts of Qizhu decoction might inhibit hepatitis and hepatocellular carcinoma by suppressing NF-κB signaling.
Assuntos
Carcinoma Hepatocelular/tratamento farmacológico , Medicamentos de Ervas Chinesas/uso terapêutico , Vírus da Hepatite B/genética , Hepatite B Crônica/tratamento farmacológico , Hepatite Animal/tratamento farmacológico , Neoplasias Hepáticas/tratamento farmacológico , Fator de Transcrição RelA/metabolismo , Animais , Carcinoma Hepatocelular/induzido quimicamente , Carcinoma Hepatocelular/patologia , Dietilnitrosamina/farmacologia , Modelos Animais de Doenças , Medicamentos de Ervas Chinesas/farmacologia , Técnicas de Silenciamento de Genes , Células Hep G2 , Hepatite B Crônica/virologia , Hepatite Animal/induzido quimicamente , Humanos , Neoplasias Hepáticas/induzido quimicamente , Neoplasias Hepáticas/patologia , Masculino , Medicina Tradicional Chinesa/métodos , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Transgênicos , Transdução de Sinais/efeitos dos fármacos , Transdução de Sinais/genética , Fator de Transcrição RelA/antagonistas & inibidores , Fator de Transcrição RelA/genética , TransfecçãoRESUMO
Based on MODIS images and by using the algorithm of maximum value composite (MVC), the monthly vegetation indices (VIs) in 2005 in Hunan Province were obtained. Through the analysis of the MODIS VIs, Hunan Province was divided into six districts to describe the spatial distribution of the VIs, and by using the monthly mean temperature and rainfall data collected from 5 climatic monitoring stations in this province, the temporal variation of the VIs was analyzed. The results showed that the spatial distribution of MODIS VIs was positively correlated with vegetation cover, and appeared regional characteristics. The MODIS VIs varied with season, and the curves of their monthly mean values were downwards opening quadratic parabolas, with the maximum appeared in July. The value of MODIS EVI was smaller than that of MODIS NDVI. MODIS VI was mainly affected by monthly mean temperature, but this effect was decreased with decreasing latitude. The variation pattern of MODIS EVI was more apparent than that of MODIS NDVI, i. e. , the quadratic parabola of MODIS EVI was smoother, going gradually from minimum to maximum and then going down, while that of MODIS NDVI had tiny fluctuations on both sides of the maximum point.