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1.
J Mol Model ; 30(11): 360, 2024 Oct 02.
Artigo em Inglês | MEDLINE | ID: mdl-39356315

RESUMO

CONTEXT: This research assesses the influence of polypropylene (PP) fibers, both homopolymer and hydroxylated (PPOH), on the tensile properties of calcium silicate hydrate (C-S-H) composites through molecular dynamics (MD) simulations. Our models explore C-S-H matrices integrated with PP and PPOH fibers at varying polymerization degrees. The results demonstrate that both PP and PPOH fibers significantly influence the tensile strength and Young's modulus of the composites. Notably, PPOH fibers contribute to more substantial mechanical enhancements than PP, attributed to the increased polarity and enhanced intermolecular interactions from the hydroxyl groups. The study reveals a nonlinear relationship between polymer additive content and mechanical performance, with optimal properties at a polymerization degree of 20. Additionally, stress-strain analysis indicates that PPOH composites exhibit superior ductility and fracture energy, particularly at polymerization degrees of 20, showing enhanced ultimate strain and fracture energy by up to 9.6% and 13.9%, respectively, compared to PP counterparts. These results highlight the crucial role of tailored polymer additive composition and chemical modifications in maximizing the mechanical efficacy of C-S-H-based materials, enhancing their durability and structural performance. METHODS: All MD simulations were conducted using LAMMPS. The models employed a combination of Clayff and Cvff force fields. During the entire tensile simulation, the system was configured under the NPT ensemble at 300 K.

2.
Artigo em Inglês | MEDLINE | ID: mdl-39265182

RESUMO

Despite extensive investigation into estrogen's role in pulmonary hypertension (PH) development, its effects-whether beneficial or detrimental-remains contentious. This study aimed to elucidate estrogen's potential role in PH under normoxic and hypoxic conditions. Utilizing norfenfluramine- and hypoxia-induced rat models of PH, the study evaluated the impact of 17ß-estradiol (E2) on PH progression. E2 promoted PH development under normoxia while providing protection under hypoxia. Mechanistically, under normoxia, E2 upregulated methyltransferase-like 3 (METTL3) gene transcription and protein via an estrogen response element-dependent pathway, which in turn elevated the m6A methylation and translational efficiency of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase isoform 3 (PFKFB3) mRNA, leading to increased PFKFB3 protein levels and enhanced proliferation and migration of pulmonary artery smooth muscle cells (PASMCs). Conversely, under hypoxia, E2 downregulated METTL3 transcription through a hypoxia response element-dependent mechanism, driven by elevated hypoxia-induced factor 1α (HIF-1α) levels, resulting in reduced PFKFB3 protein expression and diminished PASMCs proliferation and migration. Both METTL3 and PFKFB3 proteins are upregulated in the pulmonary arteries of patients with PAH. Collectively, these findings suggest that E2 exerts differential effects on PH progression via dual regulation of the METTL3/PFKFB3 protein under normoxic and hypoxic conditions, positioning the METTL3/PFKFB3 protein as a potential therapeutic target for PH treatment.

3.
Sci Data ; 11(1): 1010, 2024 Sep 18.
Artigo em Inglês | MEDLINE | ID: mdl-39294198

RESUMO

Maruca vitrata, a significant pest of legumes, impacts food security in Asia and Africa. This study presents a high-quality genome assembly of M. vitrata, utilizing advanced sequencing technologies including Nanopore long-read, MGI short-read, and Hi-C. The genome, totaling 482.3 Mb with a contig N50 of 2.91 Mb, features 41.58% repetitive sequences and encompasses 13,320 protein-coding genes. We performed comparative genomic analyses to affirm the accuracy and completeness of the protein sequences assembled, ensuring the assembly's integrity. Additionally, the annotation of 83 Cytochrome P450 (CYP) genes further confirms the comprehensive nature of the genome assembly and its annotations. This genome assembly not only deepens our understanding of M. vitrata biology but also supports the development of sustainable pest management strategies. This research highlights the importance of genomics in advancing sustainable agricultural solutions through innovative pest management approaches.


Assuntos
Genoma de Inseto , Animais , Mariposas/genética , Anotação de Sequência Molecular , Fabaceae/genética
4.
Ren Fail ; 46(2): 2403645, 2024 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-39297199

RESUMO

BACKGROUND: Chronic Kidney Disease (CKD) has emerged as a significant global health issue. This study aimed to reveal and predict the epidemiological characteristics of CKD. METHODS: Data from the Global Burden of Disease Study spanning the years 1990 to 2019 were employed to analyze the incidence, prevalence, death, and disability-adjusted life year (DALY) of CKD. Joinpoint analysis assessed epidemiological trends of CKD from 1990 to 2019. An age-period-cohort model evaluated risk variations. Risk factor analysis uncovered their influences on DALYs and deaths of CKD. Decomposition analysis explored the drivers to CKD. Frontier analysis evaluated the correlations between CKD burden and the sociodemographic index (SDI). A Bayesian Age-Period-Cohort model was employed to predict future incidence and death of CKD. RESULTS: In 2019, there were 18,986,903 incident cases, 697,294,307 prevalent cases, 1,427,232 deaths, and 41,538,592 DALYs of CKD globally. Joinpoint analysis showed increasing age-standardized rates of CKD incidence, prevalence, mortality, and DALY from 1990 to 2019. High systolic blood pressure significantly contributed to CKD-related deaths and DALYs, particularly in the high SDI region. Decomposition analysis identified population growth as the primary driver of CKD incident cases and DALYs globally. Countries like Nicaragua showed the highest effective differences, indicating room for improvement in CKD management. By 2030, while incident cases of CKD were predicted to rise, the global deaths might decrease. CONCLUSIONS: The study revealed a concerning upward trend in the global burden of CKD, emphasizing the need for targeted management strategies across different causes, regions, age groups, and genders.


Assuntos
Teorema de Bayes , Carga Global da Doença , Insuficiência Renal Crônica , Humanos , Insuficiência Renal Crônica/epidemiologia , Masculino , Feminino , Pessoa de Meia-Idade , Adulto , Idoso , Incidência , Prevalência , Fatores de Risco , Carga Global da Doença/tendências , Adulto Jovem , Anos de Vida Ajustados por Deficiência , Estudos de Coortes , Adolescente , Saúde Global/estatística & dados numéricos , Idoso de 80 Anos ou mais , Previsões , Criança
5.
J Glob Health ; 14: 04193, 2024 Sep 20.
Artigo em Inglês | MEDLINE | ID: mdl-39301589

RESUMO

Background: Prior studies exploring the impact of widowhood on cognitive impairment in later life have been focussed on the USA and Europe. We aimed to explore the mediating role of social engagement, health behaviours, and subjective well-being in the association between widowhood and cognitive impairment in the Chinese population. Methods: We conducted a study on 7796 older individuals enrolled in the 2018 wave of the Chinese Longitudinal Health Longevity Study. We used logistic regression models to analyse the impact of widowhood on cognitive health among older adults and performed mediation analysis to determine possible mediating factors in this relationship. Results: Widows and widowers had a higher risk of having cognitive impairment than married older adults (95% confidence interval (CI) = 1.312, 2.279). The results from structural equation modelling (SEM) provided a good fit to the observed data (χ2 = 24.909; P = 0.00) and indicated that the effect of widowhood on cognitive impairment was partially mediated by social engagement, lifestyle behaviours, and subjective well-being (ß = 0.075; P < 0.01). Conclusions: Our findings contribute to existing research on the mechanisms underlying the association between widowhood and cognitive impairment among older individuals, suggesting a need for policies targeted at the specific needs of this vulnerable population, such as the maintenance of social interactions, adoption of a healthy lifestyle, improvement of subjective well-being, and provision of necessary support systems.


Assuntos
Disfunção Cognitiva , Viuvez , Humanos , Viuvez/psicologia , Viuvez/estatística & dados numéricos , Feminino , Masculino , China/epidemiologia , Idoso , Disfunção Cognitiva/epidemiologia , Idoso de 80 Anos ou mais , Estudos Longitudinais , Participação Social/psicologia , Fatores de Risco , Pessoa de Meia-Idade , População do Leste Asiático
6.
Food Chem ; 463(Pt 2): 141180, 2024 Sep 07.
Artigo em Inglês | MEDLINE | ID: mdl-39276541

RESUMO

The effects of chickpea protein (CP) modified by heating and/or high-pressure homogenization (HPH) on the gelling properties of myofibrillar protein under reduced phosphate conditions (5 mM sodium triphosphate, STPP) were investigated. The results showed that heating and HPH dual-modified CP could decrease the cooking loss by 29.57 %, elevate the water holding capacity by 17.08 %, and increase the gel strength by 126.88 %, which conferred myofibrillar protein with gelation performance comparable with, or even surpassing, that of the high-phosphate (10 mM STPP) control. This gelation behavior improvement could be attributed to enhanced myosin tail-tail interactions, decreased myosin thermal stability, elevated trans-gauche-trans disulfide conformation, strengthened hydrophobic interactions and hydrogen bonding, the uncoiling of α-helical structures, the formation of well-networked myofibrillar protein gel, and the disulfide linkages between the myosin heavy chain, actin, and CP subunits. Therefore, the dual-modified CP could be a promising phosphate alternative to develop healthier meat products.

7.
Chem Biodivers ; : e202402048, 2024 Sep 18.
Artigo em Inglês | MEDLINE | ID: mdl-39291792

RESUMO

In this work, bismuth ferrites (BFO) nanoparticles were produced in the form of using sol-gel technique, followed by annealing in a tube furnace in temperatures from 400 °C to 650 ºC. X-ray diffraction (XRD) results showed the formation of small sizes nanoparticles (NPs) with high purity. Structural analysis displayed that annealing at 600 ºC could make BFO NPs be fitted to rhombohedral space group (R3c), with small quantity of spurious phases. The sizes of the BFO nanoparticles determined by transmission electron microscopy (HRTEM) are between 50 to 100 nm. To evaluate the efficiency of BFO in antimicrobial susceptibility tests, the nanoparticles were dispersed through nanoemulsion and tested agar diffusion method and dilution in a 96 well plate using a Gram positive strains (Staphylococcus aureus) and Gram negative strain (Escherichia coli). The antibacterial activity of the BFO NPs was partially tested at concentrations of 2 mg/mL with MIC greater than 60 µg/mL for both bacteria.

8.
Angew Chem Int Ed Engl ; : e202415802, 2024 Sep 18.
Artigo em Inglês | MEDLINE | ID: mdl-39292161

RESUMO

Ventricular arrhythmias (VAs) triggered by myocardial infarction (MI) are the leading cause of sudden cardiac mortality worldwide. Current therapeutic strategies for managing MI-induced VAs, such as left stellate ganglion resection and ablation, are suboptimal, highlighting the need to explore safer and more effective intervention strategies. Herein, we rationally designed two supramolecular sonosensitizers RuA and RuB, engineered through acceptor modification to generate moderate reactive oxygen species (ROS) to modulate VAs. Both RuA and RuB demonstrated high ultrasound (US)-activated ROS production efficiency, with singlet oxygen (1O2) quantum yield (ΦΔ) of 0.70 and 0.88, respectively, surpassing ligand IR1105 and the conventional sonosensitizer ICG (ΦΔ =0.40). In vitro, RuB, at a modest concentration and under US intensity notably boosts pro-survival autophagy in microglia BV2 cell. To improve in vivo stability and biocompatibility, RuB was further encapsulated into DSPE-PEG5000 to prepare RuB NPs. In vivo studies after microinjection of RuB NPs into the paraventricular nucleus and subsequent US exposure, demonstrated that RuB NPs-mediated US modulation effectively suppresses sympathetic nervous activity (SNA) and inflammatory responses, thereby preventing VAs. Importantly, no tissue injury was observed post RuB NPs-mediated US modulation. This work pioneers the design of long-wave emission supramolecular sonosensitizers, offering new insights into regulating cardiovascular diseases.

9.
Front Neurol ; 15: 1358859, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-39346768

RESUMO

Background: Cancer patients frequently suffer pain as one of their symptoms. It includes acute and chronic pain and is one of the most feared symptoms for patients. About one-third of adults actively undergoing cancer treatment suffer from pain related to their condition. Cancer pain control remains suboptimal due to a lack of assessment, knowledge, and access. Fire needle therapy, a traditional Chinese medicine, offers a potentially beneficial addition to current pain management approaches. This protocol outlines a systematic review and meta-analysis to compile evidence and examine the pain-relieving effects and safety of fire needle therapy for cancer patients. Methods and analysis: We will systematically search China National Knowledge Infrastructure (CNKI), Wanfang Database, China Biology Medicine disc (CBM), China Science and Technology Journal Database (CSTJ or VIP), PubMed, Web of Science, Embase, Cochrane Central Registry of Controlled Trials (CENTRAL), Chinese Clinical Trial Registry (Chictr), Opengrey, Worldcat, and Scopus from inception through July 2023. Random control trials (RCTs) include all types of cancer patients (age ≥ 18 years) complaining of pain. The primary outcome will be changes in pain intensity measured by Visual Analogue Scale (VAS), Numerical Rating Scale (NRS), Neuropathic Pain Scale (NPS), or Brief Pain Inventory (BPI). Secondary outcomes include quality of life (EORTC QLQ-C30 and GCQ), performance status (KPS), times of burst pain, treatment response rate, the dose reduction of analgesic drugs, and side effects rates. Utilizing the Cochrane risk bias measurement tool: Risk of Bias 2 (RoB 2), the trials' quality will be evaluated, and meta-analysis will be performed using RevMan software (version 5.4). Discussion: This systematic review will be the first comprehensive review of the literature to provide a meta-analysis of fire needle therapy for cancer pain, including only Random control trials (RCTs). For the sake of transparency and to avoid future duplication, the publication of this protocol offers a clear illustration of the procedures utilized in this evaluation. The results of our future studies may provide a new approach and theoretical basis for the treatment of cancer pain by medical oncology professionals. Systematic review registration: https://www.crd.york.ac.uk/prospero/, identifier CRD42023418609.

10.
Int Immunopharmacol ; 142(Pt B): 113221, 2024 Sep 24.
Artigo em Inglês | MEDLINE | ID: mdl-39321709

RESUMO

Programmed cell death ligand 1 (PDL1) has been implicated in immune evasion in various tumor types. The objective of this investigation was to assess the correlation between metastasis-associated interferon-induced transmembrane protein 2 (IFITM2) and PDL1, and explore their impact on tumor immunity in gastric cancer (GC). The expression of IFITM2 and PDL1 in human GC tissues was initially evaluated using The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases, as well as immunohistochemistry (IHC). Subsequently, the relationship between IFITM2 and PDL1 was analyzed through Real-time quantitative PCR (RT-qPCR) and western blotting after cell transfection and inhibitor treatment in vitro. The role of IFITM2 and PDL1 in immune killing was further elucidated in both in vitro and in vivo settings. Our study revealed frequent overexpression of IFITM2 and PDL1 in GC. Notably, IFITM2 expression was significantly associated with lymphatic metastasis, clinical stage, and poor survival. Moreover, a positive correlation between PDL1 expression and IFITM2 expression in GC was identified. The activation of tumor-derived IFITM2 was found to enhance PDL1 expression via the JAK/STAT3 pathway in human GC cells (MKN28 and MKN45), leading to apoptosis of Jurkat T cells. Furthermore, IFITM2 induced PDL1 expression in a xenograft mouse model of GC. Based on our findings, we propose that IFITM2 modulates PDL1 expression and tumor immunity through the JAK/STAT3 pathway in GC cells, highlighting the potential of IFITM2 as a therapeutic target for GC immunotherapy.

11.
Int J Biol Macromol ; 279(Pt 3): 135463, 2024 Sep 07.
Artigo em Inglês | MEDLINE | ID: mdl-39250999

RESUMO

In response to prevailing challenges encountered in electrical applications, including insufficient mechanical strength, subpar tensile properties, and limited adaptability to dynamic motion environments, we engineered a pioneering hydrogel adhesive. Simultaneously, we presented a novel interpretation of the application of ZnO in hydrogels. Our innovative approach entailed the intertwining of polyvinyl alcohol (PVA) and flexible sodium alginate (SA) double networks (DN) through cross-linking mechanisms, resulting in the formation of a hydrogen-bonding pinned DN hydrogel. This groundbreaking design substantially amplified the cohesive and adhesive properties of the hydrogel, while the incorporation of zinc oxide (ZnO) through modification served to enhance its electrical conductivity. Our hydrogel sensor demonstrated exceptional capabilities in monitoring human motion, adeptly meeting the demands of diverse motion scenarios. Furthermore, meticulous consideration had been given to the influence of perspiration on sensor performance, rendering our sensor exceptionally well-suited for real-world applications.

12.
Adv Mater ; : e2410383, 2024 Sep 17.
Artigo em Inglês | MEDLINE | ID: mdl-39286858

RESUMO

Renal sympathetic nerves play a crucial role in the pathogenesis of hypertension, and renal denervation (RDN) is a new solution for patients with refractory hypertension. However, current RDN techniques show inconsistent results in clinical application probably owing to incomplete endovascular ablation of the sympathetic nerves and a lack of measures to localize and assess efficacy. In this study, a closed-loop RDN system consisting of a sensing unit with a piezoelectric thin-film sensor (PTFS) and a treatment unit with a hollow Pd nanoparticle shell (PdNPS) with a diameter of 202.0 nm for photothermal neural ablation is constructed. The PTFS can monitor and collect arterial pulsation and blood pressure (BP) and direct PdNPS to maximize RDN. PdNPS maintains a local temperature of 58-62 °C under near-infrared-II irradiation (1,064 nm) to achieve effective RDN within a range of 90-120 s treatment window. Photothermal ablation significantly inhibits the activities of renal sympathetic nerves post-procedure and after one month and reduces the elevation of BP by > 50%. The novel closed-loop system enables safe and efficient targeting, dynamic monitoring, and ablation of the renal sympathetic nerves. This closed-loop system provides a new strategy for RDN technology and even for treating sympathetic nerve-related chronic diseases.

13.
Anal Chim Acta ; 1320: 343030, 2024 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-39142794

RESUMO

BACKGROUND: Cobalt, an essential trace element, is vital for maintaining human nervous system function, aiding in DNA synthesis, and contributing to red blood cell production. It is helpful for disease diagnosis and treatment plan evaluation by precisely monitoring its concentration changes in the human body. Despite extensive efforts made, due to its ultra-low concentration, the current limit of detection (LOD) as reported is still inadequate and cannot be satisfied with the precise clinical applications. Therefore, it is crucial to develop novel label-free sensors with high sensitivity and excellent selectivity for detecting trace amounts of Co2+. RESULTS: Here, an ultrasensitive optical fiber SPR sensor was designed and fabricated for label-free detection of Co2+ with ultra-low concentration. It is achieved by modifying the carboxyl-functionalized CQDs on the AuNPs/Au film-coated hetero-core fiber, which can specifically capture the Co2+, leading to changes in the fiber's surface refractive index (RI) and subsequent SPR wavelength shifts in the transmission spectrum. Both the Au film and AuNPs on the fiber are modified with CQDs, leveraging their large surface area to enhance the number of active sites and probes. The sensor exhibits an ultra-high sensitivity of approximately 6.67 × 1019 nm/M, and the LOD is obtained as low as 5.36 × 10-20 M which is several orders of magnitude lower compared to other conventional methods. It is also experimentally demonstrated that the sensor possesses excellent specificity, stability, and repeatability, which may be adapted for detecting real clinical samples. SIGNIFICANCE: The CQDs-functionalized optical fiber SPR sensor exhibits substantial potential for precisely detecting Co2+ of trace amounts, which is especially vital for scarce clinical samples. Additionally, the sensing platform with sample sensor fabrication and measurement configuration introduces a novel, highly sensitive approach to biochemical analysis, particularly adapting for applications involving the detection of trace targets, which could also be employed to detect various biochemical targets by facile modification of CQDs with specific groups or biomolecules.


Assuntos
Cobalto , Ouro , Limite de Detecção , Nanopartículas Metálicas , Fibras Ópticas , Ressonância de Plasmônio de Superfície , Cobalto/química , Ouro/química , Nanopartículas Metálicas/química , Humanos
14.
Heliyon ; 10(15): e35596, 2024 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-39166003

RESUMO

An efficient and rapid method for the detection of total soluble protein in tobacco leaves, utilizing a smartphone-based colorimetric approach has been developed. The proposed low-cost, immediate, general-purpose, and high-throughput (LIGHt) smartphone colorimetric screening assay integrates commercially available microplates, enabling on-site, high-throughput screening of tobacco leaf quality. The study involves preparing protein standard solutions and constructing standard curves using both spectrophotometric and smartphone-based methods. The LIGHt smartphone colorimetry yielded an average relative standard deviation of 10.6 %, a limit of detection of 2 µg/mL, and an average recovery of 93 %. The results demonstrated a comparable performance between intensities from the blue channel and the absorbance values in reflecting protein concentrations, validating the feasibility of utilizing smartphone colorimetry for protein concentration determination. Our approach demonstrates the potential for practical implementation in the field, providing a cost-effective and user-friendly solution for rapid quality assessment in the tobacco industry. The LIGHt smartphone colorimetry enhances quality control practices in the tobacco sector and offers a promising tool for on-site production quality testing in various industries, such as fruits and vegetables.

15.
Regen Med ; 19(5): 257-278, 2024 05 03.
Artigo em Inglês | MEDLINE | ID: mdl-39118532

RESUMO

Bioactive ceramics, primarily consisting of bioactive glasses, glass-ceramics, calcium orthophosphate ceramics, calcium silicate ceramics and calcium carbonate ceramics, have received great attention in the past decades given their biocompatible nature and excellent bioactivity in stimulating cell proliferation, differentiation and tissue regeneration. Recent studies have tried to combine bioactive ceramics with bioactive ions, polymers, bioactive proteins and other chemicals to improve their mechanical and biological properties, thus rendering them more valid in tissue engineering scaffolds. This review presents the beneficial properties and potential applications of bioactive ceramic-based materials in dentistry, particularly in the repair and regeneration of dental hard tissue, pulp-dentin complex, periodontal tissue and bone tissue. Moreover, greater insights into the mechanisms of bioactive ceramics and the development of ceramic-based materials are provided.


[Box: see text].


Assuntos
Materiais Biocompatíveis , Cerâmica , Regeneração , Cerâmica/química , Cerâmica/farmacologia , Humanos , Regeneração/efeitos dos fármacos , Materiais Biocompatíveis/química , Materiais Biocompatíveis/farmacologia , Engenharia Tecidual/métodos , Animais
17.
PLoS Negl Trop Dis ; 18(8): e0012437, 2024 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-39208380

RESUMO

Hemorrhagic fever with renal syndrome caused by hantaviruses has long been a serious public health issue in Yunnan Province. Hantaviruses exhibit a high extent of biodiversity in their natural hosts, particularly in mammalian hosts. This study was conducted to screen for hantaviruses in bats and rodents in Yunnan Province and elucidate their genetic characteristics and possible zoonotic disease risk. Hantaviruses were detected in 202 bats and 372 rodents with the positive rates 27.49% and 1.25% respectively. A novel lineage (named Lineage 10) of the Seoul virus (SEOV) from rodents and the geographic clustering of hantavirus in bats were identified using phylogenetic analyses of the full-length M- and S-segments. Our study suggest a high cross-species transmissibility of hantaviruses in bats and existence of a new lineage of SEOV in rodents differing significantly from other SEOVs. These results provide data to support the prevention and control of hantavirus-associated diseases in Yunnan Province.


Assuntos
Quirópteros , Orthohantavírus , Filogenia , Roedores , Animais , Quirópteros/virologia , Roedores/virologia , China/epidemiologia , Orthohantavírus/genética , Orthohantavírus/classificação , Orthohantavírus/isolamento & purificação , Infecções por Hantavirus/virologia , Infecções por Hantavirus/veterinária , Infecções por Hantavirus/epidemiologia , Vírus Seoul/genética , Vírus Seoul/isolamento & purificação , Vírus Seoul/classificação
18.
Nat Commun ; 15(1): 6512, 2024 Aug 02.
Artigo em Inglês | MEDLINE | ID: mdl-39095395

RESUMO

Many disease resistance genes have been introgressed into wheat from its wild relatives. However, reduced recombination within the introgressed segments hinders the cloning of the introgressed genes. Here, we have cloned the powdery mildew resistance gene Pm13, which is introgressed into wheat from Aegilops longissima, using a method that combines physical mapping with radiation-induced chromosomal aberrations and transcriptome sequencing analysis of ethyl methanesulfonate (EMS)-induced loss-of-function mutants. Pm13 encodes a kinase fusion protein, designated MLKL-K, with an N-terminal domain of mixed lineage kinase domain-like protein (MLKL_NTD domain) and a C-terminal serine/threonine kinase domain bridged by a brace. The resistance function of Pm13 is validated through transient and stable transgenic complementation assays. Transient over-expression analyses in Nicotiana benthamiana leaves and wheat protoplasts reveal that the fragment Brace-Kinase122-476 of MLKL-K is capable of inducing cell death, which is dependent on a functional kinase domain and the three α-helices in the brace region close to the N-terminus of the kinase domain.


Assuntos
Aegilops , Ascomicetos , Resistência à Doença , Doenças das Plantas , Proteínas de Plantas , Triticum , Triticum/microbiologia , Triticum/genética , Doenças das Plantas/microbiologia , Doenças das Plantas/imunologia , Doenças das Plantas/genética , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Resistência à Doença/genética , Aegilops/genética , Aegilops/metabolismo , Plantas Geneticamente Modificadas , Proteínas Quinases/metabolismo , Proteínas Quinases/genética , Proteínas Recombinantes de Fusão/metabolismo , Proteínas Recombinantes de Fusão/genética , Nicotiana/genética , Nicotiana/microbiologia , Folhas de Planta/microbiologia , Folhas de Planta/genética , Folhas de Planta/metabolismo , Regulação da Expressão Gênica de Plantas
19.
J Environ Manage ; 367: 122079, 2024 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-39106800

RESUMO

Trade network connects enterprises together, while its impact on corporate ESG performance is unknown. Drawing on unbalanced panel data of resource-based enterprises in China from 2009 to 2021, this study decomposes the trade network into purchasing network and sales network based on trade directions, and explores their impact on corporate ESG performance and the mechanisms involved. The findings indicate that: (1) The trade networks exhibit a negative correlation with corporate ESG performance. Meanwhile, purchasing network demonstrates a more pronounced adverse impact on corporate ESG than sales network. After a series of endogeneity tests, the above results still hold. (2) Heterogeneity analysis shows that trade networks have a more significant effect on the ESG performance of state-owned enterprises, mature enterprises and heavily polluting enterprises. And the most pronounced negative effect is observed in the governance (G) component of ESG. (3) Mechanism analysis reveals that trade networks negatively influence corporate ESG performance by impeding the progress of green innovation and internal control. (4) Accelerating digital transformation and intensifying government environmental regulations can mitigate the inhibitory impact of trade networks on corporate ESG performance. This study provides theoretical support and empirical evidence for resource-based enterprises to enhance ESG performance while reinforcing trade linkages.


Assuntos
Comércio , China , Conservação dos Recursos Naturais , População do Leste Asiático
20.
Polymers (Basel) ; 16(16)2024 Aug 22.
Artigo em Inglês | MEDLINE | ID: mdl-39204594

RESUMO

In this paper, we propose an innovative light-powered LCE-slider system that enables continuous self-circling on an elliptical track and is comprised of a light-powered LCE string, slider, and rigid elliptical track. By formulating and solving dimensionless dynamic equations, we explain static and self-circling states, emphasizing self-circling dynamics and energy balance. Quantitative analysis reveals that the self-circling frequency of LCE-slider systems is independent of the initial tangential velocity but sensitive to light intensity, contraction coefficients, elastic coefficients, the elliptical axis ratio, and damping coefficients. Notably, elliptical motion outperforms circular motion in angular velocity and frequency, indicating greater efficiency. Reliable self-circling under constant light suggests applications in periodic motion fields, especially celestial mechanics. Additionally, the system's remarkable adaptability to a wide range of curved trajectories exemplifies its flexibility and versatility, while its energy absorption and conversion capabilities position it as a highly potential candidate for applications in robotics, construction, and transportation.

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