Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 5 de 5
Filtrar
Mais filtros

Base de dados
Tipo de documento
Intervalo de ano de publicação
1.
Food Chem ; 461: 140858, 2024 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-39173258

RESUMO

Thaumatin-like proteins (TLP), existing in various fruits, have allergenic and pro-inflammatory activities. The current research attempts to reduce the pro-inflammatory activity of litchi TLP (LcTLP) through high hydrostatic pressure (HHP). This study demonstrated that HHP (250-500 MPa, 5-10 min) was a potential technique to reduce the pro-inflammatory activity of LcTLP, which was attributed to the irreversible destruction of the active domain, ie., V-cleft. SDS-PAGE showed no change in the protein profile. Continuous HHP treatment promoted LcTLP unfolding and then reassembling (400 MPa was the transition pressure), and the content of ß-sheets decreased from 35.4% to 31.1%. HHP treatment could mitigate inflammatory responses of LcTLP, as confirmed by ELISA and western blot. Molecular dynamics simulations showed significant changes in the residue network under HHP, thereby affecting the V-cleft. These findings provide a theoretical explanation and structural insights into the HHP-induced reduction of pro-inflammatory activity of LcTLP.


Assuntos
Pressão Hidrostática , Inflamação , Litchi , Proteínas de Plantas , Proteínas de Plantas/química , Proteínas de Plantas/imunologia , Litchi/química , Inflamação/imunologia , Animais , Camundongos , Simulação de Dinâmica Molecular , Frutas/química , Frutas/imunologia , Células RAW 264.7 , Domínios Proteicos , Humanos
2.
Food Funct ; 15(9): 4818-4831, 2024 May 07.
Artigo em Inglês | MEDLINE | ID: mdl-38606579

RESUMO

Gamma-aminobutyric acid (GABA) is the predominant amino acid in litchi pulp, known for its neuroregulatory effects and anti-inflammatory properties. Although previous research has highlighted the pro-inflammatory characteristics of litchi thaumatin-like protein (LcTLP), interplay between GABA and LcTLP in relation to inflammation remains unclear. This study aims to explore the hepatoprotective effects of the litchi pulp-derived GABA extract (LGE) against LcTLP-induced liver inflammation in mice and LO2 cells. In vivo experiments demonstrated that LGE significantly reduced the levels of aspartate transaminase and alanine transaminase, and protected the liver against infiltration of CD4+ and CD8+ T cells and histological injury induced by LcTLP. Pro-inflammatory cytokines including interleukin-6, interleukin-1ß, and tumor necrosis factor-α were also diminished by LGE. The LGE appeared to modulate the mitogen-activated protein kinase (MAPK) signaling pathway to exert its anti-inflammatory effects, as evidenced by a reduction of 47%, 35%, and 31% in phosphorylated p38, JNK, and ERK expressions, respectively, in the liver of the high-dose LGE group. Additionally, LGE effectively improved the translocation of gut microbiota by modulating its microbiological composition and abundance. In vitro studies have shown that LGE effectively counteracts the increase in reactive oxygen species, calcium ions, and pro-inflammatory cytokines induced by LcTLP. These findings may offer new perspectives on the health benefits and safety of litchi consumption.


Assuntos
Litchi , Extratos Vegetais , Ácido gama-Aminobutírico , Animais , Camundongos , Litchi/química , Extratos Vegetais/farmacologia , Masculino , Ácido gama-Aminobutírico/metabolismo , Fígado/efeitos dos fármacos , Fígado/metabolismo , Citocinas/metabolismo , Anti-Inflamatórios/farmacologia , Proteínas de Plantas/farmacologia , Inflamação/tratamento farmacológico , Microbioma Gastrointestinal/efeitos dos fármacos , Humanos , Camundongos Endogâmicos C57BL , Frutas/química , Aspartato Aminotransferases
3.
PLoS One ; 17(3): e0265457, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35294498

RESUMO

ε-Poly-l-lysine (ε-PL) is a natural antimicrobial polymer with significant inhibitory activity against a broad spectrum of microorganisms, and nowadays used widely as a preservative in the food industry. In the present study, ε-PL broth was obtained from Streptomyces ahygroscopicus GIM8 fermentation in a nutrient-limited liquid medium. The in vitro antifungal activity of the broth against fruit pathogens Penicillium expansum and Colletotrichum gloeosporioides was investigated, and its usage for postharvest storage of two highly perishable fruits wax apple and guava was evaluated. Results showed that ε-PL concentration in the broth reached 0.61 g/L, and the nutrition level of the broth was low. The antifungal activity of ε-PL broth was comparable to that of the aqueous solution of ε-PL under the same concentration. Immersion with the diluted broth (200 mg/L ε-PL) markedly delayed the decline in the quality of postharvest wax apple and guava fruits during storage, and the decay incidences were also greatly decreased as compared to their respective controls (distilled water immersion). A further investigation demonstrated that the ε-PL broth immersion induced an increase in the activity of defense-related enzymes peroxidase and polyphenol oxidase in the two fruits during storage. The present study proved that the fermentation broth of ε-PL could be used as a promising alternative to high purity ε-PL and synthetic fungicides for preserving fruits at postharvest stage.


Assuntos
Psidium , Streptomyces , Syzygium , Antifúngicos/farmacologia , Frutas/microbiologia , Polilisina/farmacologia
4.
J Nat Prod ; 85(2): 375-383, 2022 02 25.
Artigo em Inglês | MEDLINE | ID: mdl-35171609

RESUMO

Eight new 2,6-disubstituted piperidin-3-ol alkaloids (1-8), featuring a C10 unsaturated alkyl side chain, together with three previously reported analogues (9-11) were isolated from the leaves of medicinal plant Microcos paniculata. Their structures and absolute configurations were elucidated unambiguously by means of 1D and 2D NMR spectroscopic data analysis, modified Mosher's method, Snatzke's method, and quantum chemical electronic circular dichroism (ECD) calculations, as well as single-crystal X-ray crystallography. The isolates were evaluated for their antiangiogenic effects on human umbilical vein endothelial cells (HUVECs). Compound 2 displayed an inhibitory effect on tube formation of HUVECs in a concentration-dependent manner.


Assuntos
Alcaloides , Malvaceae , Alcaloides/química , Dicroísmo Circular , Células Endoteliais , Humanos , Estrutura Molecular , Piperidinas/química , Piperidinas/farmacologia , Folhas de Planta/química
5.
Zhongguo Zhong Yao Za Zhi ; 39(12): 2199-203, 2014 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-25244744

RESUMO

ITS2 sequence was used as a barcode to identify herbal tea ingredient Plumeria rubra and its adulterants. Genomic DNAs from forty eight samples were extracted, the ITS2 sequences were amplified and sequenced bi-direstionlly, and then assembled and obtained using CodonCode Aligner. The sequences were aligned using ClustalW, the genetic distances were computed by kimura 2-parameter (K2P) model and the Neighbor-joining (NJ) phylogenetic trees were constructed using MEGA5.0. Results showed that the length of ITS2 sequence of P. rubra were 244 bp. The intra-specific genetic distances (0-0. 016 6) were much smaller than inter-specific ones between P. rubra and its adulterants(0.320 8-0.650 4). The NJ tree indicated that P. rubra and its adulterants could be distinguished clearly. Therefore, Using ITS2 barcode can accurately andeffectively distinguish herbal tea ingredient P. rubra from its adulterants, which providesa new molecular method to identify P. rubra and ensure its safety in use.


Assuntos
Apocynaceae/classificação , Código de Barras de DNA Taxonômico/métodos , Medicamentos de Ervas Chinesas/classificação , Apocynaceae/genética , DNA de Plantas/genética , DNA Espaçador Ribossômico/genética , Contaminação de Medicamentos/prevenção & controle , Medicamentos de Ervas Chinesas/química , Flores/química , Flores/classificação , Dados de Sequência Molecular , Filogenia , Controle de Qualidade
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA