RESUMO
T cell neonatal tolerance to self evolves perturbation of the Th1/Th2 balance towards Th2-type self-specific T cells. In the current study we have demonstrated that a tolerant state could be extended to another encephalitogenic determinant only if the neonatally tolerizing determinant was co-administered in adult life with an emulsion of Mycobacterium tuberculosis (i.e. complete Freund's adjuvant). The mechanisms underlying tolerance elicitation and expansion were then explored by an in vitro system in which indirect suppression could be measured. Addition of a tolerizing epitope to splenic T cells from neonatally tolerized animals induced a marked suppression of the anti-MT response. This response could be restored by neutralizing antibodies to IL-4. In contrast, the neutralizing antibodies to IL-4 had no affect on the response of these cells to the tolerizing determinant. These findings are highly significant not only because they explore the important role of microbial antigens in neonatal tolerance, but also because they distinguish, for the first time, between tolerizing and tolerized T cells.
Assuntos
Envelhecimento/imunologia , Animais Recém-Nascidos/crescimento & desenvolvimento , Animais Recém-Nascidos/imunologia , Adjuvante de Freund/imunologia , Mycobacterium tuberculosis/imunologia , Tolerância a Antígenos Próprios/fisiologia , Sequência de Aminoácidos , Animais , Encefalomielite Autoimune Experimental/imunologia , Feminino , Ativação Linfocitária , Dados de Sequência Molecular , Ratos , Ratos Endogâmicos Lew , Células Th1/imunologia , Células Th2/imunologiaRESUMO
Structuredness of the cytoplasmic matrix (SCM)-responding lymphocytes from healthy donors can be differentiated from SCM-responding lymphocytes of patients with malignant disease on the basis of the change in the intracellular fluorescein fluorescence polarization (IFFP) induced by their exposure to various antigens. We have found that the overall sensitivity, specificity and positive and negative predictive values of the test were 92.0, 92.6, 96.3, and 84.7%, respectively. We demonstrated the capability of the test to distinguish between healthy people and colorectal cancer patients per stage of the disease. We also found a significant difference in IFFP values between Dukes' C patients and patients with metastatic disease, rendering the test potentially helpful in follow-up.
Assuntos
Neoplasias Colorretais/diagnóstico , Linfócitos/química , Adulto , Idoso , Idoso de 80 Anos ou mais , Citoplasma/química , Feminino , Polarização de Fluorescência , Humanos , Masculino , Pessoa de Meia-Idade , Metástase Neoplásica , Estudos Retrospectivos , Sensibilidade e EspecificidadeRESUMO
Neutropenic colitis (NC) is an inflammatory process of the terminal ileum, cecum and ascending colon which often develops in neutropenic patients following chemotherapy for malignant diseases. A case of NC in 65-year old man who was neutropenic after chemotherapy for chronic lymphatic leukemia, is presented. Early diagnosis on CT-scan and appropriate antibiotic therapy led to prompt resolution of the bowel condition. Physicians who treat patients with malignant diseases should be aware of this potentially lethal condition so that it can be effectively diagnosed and treated.