RESUMO
Background. Two non-tuberculous mycobacterial strains, UM_3 and UM_11, were isolated from the trunk wash of captive elephants in Malaysia. As they appeared to be identical phenotypes, they were investigated further by conventional and whole genome sequence-based methods of strain differentiation. Methods. Multiphasic investigations on the isolates included species identification with hsp65 PCR-sequencing, conventional biochemical tests, rapid biochemical profiling using API strips and the Biolog Phenotype Microarray analysis, protein profiling with liquid chromatography-mass spectrometry, repetitive sequence-based PCR typing and whole genome sequencing followed by phylogenomic analyses. Results. The isolates were shown to be possibly novel slow-growing schotochromogens with highly similar biological and genotypic characteristics. Both strains have a genome size of 5.2 Mbp, G+C content of 68.8%, one rRNA operon and 52 tRNAs each. They qualified for classification into the same species with their average nucleotide identity of 99.98% and tetranucleotide correlation coefficient of 0.99999. At the subspecies level, both strains showed 98.8% band similarity in the Diversilab automated repetitive sequence-based PCR typing system, 96.2% similarity in protein profiles obtained by liquid chromatography mass spectrometry, and a genomic distance that is close to zero in the phylogenomic tree constructed with conserved orthologs. Detailed epidemiological tracking revealed that the elephants shared a common habitat eight years apart, thus, strengthening the possibility of a clonal relationship between the two strains.
RESUMO
Members of the Mycobacterium terrae complex are slow-growing, non-chromogenic acid-fast bacilli found in the natural environment and occasionally in clinical material. These genetically closely-related members are difficult to differentiate by conventional phenotypic and molecular tests. In this paper we describe the use of whole genome data for the identification of four strains genetically similar to Mycobacterium sp. JDM601, a newly identified member of the M. terrae complex. Phylogenetic information from the alignment of genome-wide orthologous genes and single nucleotide polymorphisms show consistent clustering of the four strains together with M. sp. JDM601 into a distinct clade separate from other rapid and slow growing mycobacterial species. More detailed inter-strain comparisons using average nucleotide identity, tetra-nucleotide frequencies and analysis of synteny indicate that our strains are closely related to but not of the same species as M. sp. JDM601. Besides the 16S rRNA signature described previously for the M. terrae complex, five more hypothetical proteins were found that are potentially useful for the rapid identification of mycobacterial species belonging to the M. terrae complex. This paper illustrates the versatile utilization of whole genome data for the delineation of new bacterial species and introduces four new genomospecies to add to current members in the M. terrae complex.
Assuntos
Genômica , Micobactérias não Tuberculosas/genética , Micobactérias não Tuberculosas/isolamento & purificação , Animais , Sequência de Bases , Genoma Bacteriano/genética , Dados de Sequência Molecular , Micobactérias não Tuberculosas/classificação , Filogenia , Especificidade da EspécieRESUMO
Mycobacterium massiliense is a nontuberculous mycobacterium associated with human infections. We report here the draft genome sequence of M. massiliense strain M159, isolated from the bronchial aspirate of a patient who had a pulmonary infection. This strain showed genotypic and in vitro resistance to a number of tetracyclines and beta-lactam antibiotics.
RESUMO
Mycobacterium abscessus is an emerging clinical pathogen commonly associated with non-tuberculous mycobacterial infections. We report herein the draft genome of M. abscessus strain M156.
RESUMO
A patient with Mycobacterium abscessus lung disease was mistaken to have pulmonary tuberculosis with airway colonization by the non-tuberculous mycobacterium. Appropriate antibiotics were only given when the patient's signs and symptoms worsened while on anti-tuberculosis therapy. Despite treatment with a combination of antibiotics showing in vitro susceptibility, the pathogen persisted in the respiratory secretions for longer than 6 months and the patient suffered a spontaneous pneumothorax 14 months into treatment. This case illustrates the chronic course of M. abscessus lung infection, the tendency for flare-ups, the inadequacy of current treatment regimens, and the necessity for prolonged patient follow-up.
RESUMO
Mycobacterium abscessus is an environmental bacterium with increasing clinical relevance. Here, we report the annotated whole-genome sequence of M. abscessus strain M152.
Assuntos
DNA Bacteriano/química , DNA Bacteriano/genética , Genoma Bacteriano , Mycobacterium/genética , Análise de Sequência de DNA , Microbiologia Ambiental , Humanos , Dados de Sequência Molecular , Mycobacterium/isolamento & purificação , Infecções por Mycobacterium/microbiologiaRESUMO
Mycobacterium abscessus is a ubiquitous, rapidly growing species of nontuberculous mycobacteria that colonizes organic surfaces and is frequently associated with opportunistic infections in humans. We report here the draft genome sequence of Mycobacterium abscessus strain M139, which shows genomic features reported to be characteristic of both Mycobacterium abscessus subsp. abscessus and Mycobacterium abscessus subsp. massiliense.
Assuntos
DNA Bacteriano/química , DNA Bacteriano/genética , Genoma Bacteriano , Mycobacterium/genética , Análise de Sequência de DNA , Humanos , Masculino , Dados de Sequência Molecular , Mycobacterium/isolamento & purificação , Infecções por Mycobacterium/microbiologia , Nepal , Escarro/microbiologiaRESUMO
Mycobacterium abscessus is a species of rapidly growing nontuberculous mycobacteria that is frequently associated with opportunistic infections in humans. Here, we report the annotated genome sequence of M. abscessus strain M94, which showed an unusual cluster of tRNAs.
Assuntos
DNA Bacteriano/química , DNA Bacteriano/genética , Genoma Bacteriano , Família Multigênica , Mycobacterium/genética , RNA de Transferência/genética , Análise de Sequência de DNA , Humanos , Dados de Sequência Molecular , Mycobacterium/isolamento & purificação , Infecções por Mycobacterium não Tuberculosas/microbiologia , Infecções Oportunistas/microbiologia , RNA Bacteriano/genéticaRESUMO
Mycobacterium massiliense has recently been proposed as a member of Mycobacterium abscessus subsp. bolletii comb. nov. Strain M154, a clinical isolate from the bronchoalveolar lavage fluid of a Malaysian patient presenting with lower respiratory tract infection, was subjected to shotgun DNA sequencing with the Illumina sequencing technology to obtain whole-genome sequence data for comparison with other genetically related strains within the M. abscessus species complex.
Assuntos
Genoma Bacteriano , Micobactérias não Tuberculosas/genética , Técnicas de Tipagem Bacteriana , Sequência de Bases , Líquido da Lavagem Broncoalveolar/microbiologia , Mapeamento Cromossômico , DNA Bacteriano/genética , Humanos , Malásia , Anotação de Sequência Molecular , Dados de Sequência Molecular , Infecções por Mycobacterium não Tuberculosas/microbiologia , Micobactérias não Tuberculosas/classificação , Micobactérias não Tuberculosas/isolamento & purificação , Infecções Respiratórias/microbiologia , Análise de Sequência de DNARESUMO
We report the draft genome sequence of a clinical isolate, strain M115, identified as Mycobacterium massiliense, a member of the newly created taxon of Mycobacterium abscessus subspecies bolletii comb. nov.
Assuntos
Genoma Bacteriano , Micobactérias não Tuberculosas/genética , Escarro/microbiologia , Técnicas de Tipagem Bacteriana , Sequência de Bases , Mapeamento Cromossômico , DNA Bacteriano/genética , Humanos , Dados de Sequência Molecular , Micobactérias não Tuberculosas/classificação , Micobactérias não Tuberculosas/isolamento & purificação , Análise de Sequência de DNARESUMO
The genome of Mycobacterium massiliense M172, isolated from a human sputum sample, was sequenced using Illumina GA IIX technology and found to contain 5,204,460 bp, including putative genes for virulence and antibiotic resistance as well as a 92-kb genomic region most likely to correspond to a mycobacteriophage.
Assuntos
DNA Bacteriano/química , DNA Bacteriano/genética , Genoma Bacteriano , Mycobacterium/genética , Análise de Sequência de DNA , Farmacorresistência Bacteriana , Humanos , Dados de Sequência Molecular , Micobacteriófagos/genética , Mycobacterium/isolamento & purificação , Mycobacterium/patogenicidade , Mycobacterium/virologia , Infecções por Mycobacterium/microbiologia , Escarro/microbiologia , Fatores de Virulência/genéticaRESUMO
Mycobacterium massiliense is a rapidly growing mycobacterial species. The pathogenicity of this subspecies is not well known. We report here the annotated genome sequence of M. massiliense strain M18, which was isolated from a lymph node biopsy specimen from a Malaysian patient suspected of having tuberculous cervical lymphadenitis.
Assuntos
DNA Bacteriano/química , DNA Bacteriano/genética , Genoma Bacteriano , Mycobacterium/genética , Análise de Sequência de DNA , Biópsia , Humanos , Linfonodos/microbiologia , Malásia , Dados de Sequência Molecular , Mycobacterium/isolamento & purificação , Tuberculose dos Linfonodos/microbiologiaRESUMO
The whole-genome sequence of Mycobacterium bolletii M24, isolated from the bronchoalveolar lavage fluid of a Malaysian patient, is reported here. The circular chromosome of 5,507,730 bp helped to clarify the taxonomic position of this organism within the M. abscessus complex and revealed the presence of proteins potentially important for pathogenicity in a human host.
Assuntos
DNA Bacteriano/química , DNA Bacteriano/genética , Genoma Bacteriano , Mycobacterium/genética , Análise de Sequência de DNA , Líquido da Lavagem Broncoalveolar/microbiologia , Cromossomos Bacterianos , Humanos , Malásia , Dados de Sequência Molecular , Mycobacterium/classificação , Mycobacterium/isolamento & purificação , Infecções por Mycobacterium não Tuberculosas/microbiologiaRESUMO
Mycobacterium abscessus is a rapid-growing species of nontuberculous mycobacteria that is frequently associated with opportunistic infections in humans. We report herein the draft genome sequence of M. abscessus strain M93.