Fabrication of a ratiometric electrochemiluminescence biosensor using single self-enhanced nanoluminophores for the detection of spermine.

A ratiometric electrochemiluminescence strategy using a single luminophore for accurate and sensitive biomolecule detection could be immensely valuable in bioanalysis. Herein, an ultrasensitive ratiometric electrochemiluminescence sensing system was fabricated using a self-enhanced luminophore with dual-signal emission for the detection of spermine. A nanocomposite was synthesized by the covalent attachment of N, N-diisopropylethylenediamine onto glutathione-protected Au-Ag bimetallic nanoclusters (DPEA-GSH@Au/Ag BNCs). The nanocomposite exhibited efficient intra-cluster charge transfer to produce strong anodic self-enhanced electrochemiluminescence emission at 0.8 V without external co-reactants. Interestingly, the DPEA@GSH@Au-Ag BNCs exhibited cathodic electrochemiluminescence emission upon the addition of the co-reactant potassium persulfate at -1.6 V, exhibiting stable and efficient dual-signal electrochemiluminescence emission features at a continuous potential window of -1.75 to 1.2 V. Thus, they were used to fabricate a single-luminophore electrochemiluminescence sensor with dual emission. The cathodic emission of the biosensor gradually increased with increasing concentrations of spermine, whereas the anodic electrochemiluminescence intensity remained almost constant, enabling the ratiometric detection of spermine. The fabricated biosensor, with an internal standard, significantly improved the accuracy and reliability of spermine detection in a wide concentration range of 0.85 pM-100 µM, with a low limit of detection of 0.12 pM (S/N = 3) under optimum conditions. This single-luminophore electrochemiluminescence sensing system could be used for the detection of spermine and could guide the construction of ratiometric electrochemiluminescence sensors in the future.

RanBP2/Nup358 Mediates Sumoylation of STAT1 and Antagonizes Interferon-α-Mediated Antiviral Innate Immunity.

Type I interferon (IFN-I)-induced signaling plays a critical role in host antiviral innate immune responses. Despite this, the mechanisms that regulate this signaling pathway have yet to be fully elucidated. The nucleoporin Ran Binding Protein 2 (RanBP2) (also known as Nucleoporin 358 KDa, Nup358) has been implicated in a number of cellular processes, including host innate immune signaling pathways, and is known to influence viral infection. In this study, we documented that RanBP2 mediates the sumoylation of signal transducers and activators of transcription 1 (STAT1) and inhibits IFN-α-induced signaling. Specifically, we found that RanBP2-mediated sumoylation inhibits the interaction of STAT1 and Janus kinase 1 (JAK1), as well as the phosphorylation and nuclear accumulation of STAT1 after IFN-α stimulation, thereby antagonizing the IFN-α-mediated antiviral innate immune signaling pathway and promoting viral infection. Our findings not only provide insights into a novel function of RanBP2 in antiviral innate immunity but may also contribute to the development of new antiviral therapeutic strategies.

The Influence of Carbon Sources on the Microstructures of In Situ-Synthesized TiC in Al Melts.

In this work, graphite and diamond are successfully introduced into Al melts and TiC is in situ-synthesized based on reactive wetting. It is found that the microstructures of the prepared TiC-reinforced Al composites are varied with the change in carbon sources and their sizes. TiC particles tend to form agglomerations in the composites prepared by both graphite and diamond, but the size of the TiC particles as well as their agglomerations will decrease with the decrease in the carbon source size. In addition, the Ti-C reaction is also difficult to fully carry out due to the influence of the Al-C reaction. As a result, in addition to TiC particles, Al4C3 will also be present in the composites prepared by graphite, especially when the size of graphite is large. As for the composites prepared by diamond, diamond@Al4C3@TiC core-shell particles will form when the size of the diamond is large, such as 10 µm in this work, and these particles will transform into Al4C3@TiC core-shell particles when the size of the diamond is decreased.

Triggered peroxidase-like activity of Au decorated carbon dots for colorimetric monitoring of Hg2+ enrichment in Chlorella vulgaris.

Developing a rapid, low-cost, and multimode detection method for heavy metal ions remains a compelling goal for many applications, including food safety, environmental and biological analysis. This study investigated the influence of Hg2+ on the peroxidase-like activity of gold nanoparticles (GNPs) decorated on carbon dots (CDs) from lysine (denoted as GNP@CDs). A new type of Hg2+-triggered peroxidase-like activity of GNP@CDs was discovered, which could catalyze the oxidation of the colorless 3,3',5,5'-tetramethylbenzidine (TMB) into blue TMB. Based on the regulation of the catalytically triggered activity, a sensitive colorimetric method for the detection of Hg2+ was developed, with a linear range of 7-150 nM, providing a limit of detection as low as 3.7 nM. The sensor is simple and rapid, and was successfully applied to the detection of Hg2+ enrichment in chlorella, suggesting a promising application in biological analysis.