Analyzing the impact of sustainable economic development from the policy text network: Based on the practice of China's bay area policy.

In order to break through the surface analysis of the content structure of policy texts, an in-depth discussion of the linkage between regional policy makers and objectives is helpful to analyze the formation mechanism of policy effects. Through social network analysis and multi-index analysis, this study takes the QianwanNew Area of Ningbo and the Guangdong-Hong Kong-Macao Greater Bay Area as representatives to explore the policy framework for the sustainable development of manufacturing industry in the two bay areas respectively. Through the construction of government department cooperation network, policy keyword co-occurrence network, department keyword correlation network, and the analysis of network density, network centrality, structural holes, and cohesive subgroups, it is found that the impact results show great differences, which is related to the network structure of manufacturing policy text.

PGC-1α-siRNA suppresses inflammation in substantia nigra of PD mice by inhibiting microglia.

Background and purpose: Parkinson's disease is a common degenerative disease of the central nervous system with complex pathogenesis. More and more studies have found that inflammatory response promotes the occurrence and development of the disease, in which the activation of microglia plays an important role. PGC-1α (peroxisome proliferator activated receptor-γ coactivator-1α) is the main factor in mitochondrial biogenetic, and is closely related to the inflammatory response. Our immunofluorescence test results showed that PGC-1α and microglia (Iba1) have double-labeled phenomenon. The expression of microglia in the MPTP (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine) group increased, and PGC-1α/Iba1 double label increased. To test whether lowering the expression of PGC-1α can reduce the activation of microglia and protect the substantia nigra dopaminergic neurons, we constructed PGC-1α interference lentivirus.Methods: Immunofluorescence, western blot, and ELISA were used to detect microglial phenotype.Results: The results showed that PGC-1α interfering with lentivirus can transfect microglial cells in substantia nigra, and the PGC-1α protein level decreased in substantia nigra accordingly; TH protein expression had no statistical difference compared with MPTP group; PGC-1α interfering lentivirus reduced microglia number and activation, and at the same time the expression of iNOS and Arg1 significantly reduced compared with MPTP group. The IL-6 expression in blood detected using ELISA was significantly reduced compared with MPTP group.Conclusion: PGC-1α downregulation inhibited microglia activity, and both M1 and M2 microglial activities are reduced.

Liraglutide Regulates Mitochondrial Quality Control System Through PGC-1α in a Mouse Model of Parkinson's Disease.

Parkinson's disease (PD) is a multifactorial disorder, and there is strong evidence that mitochondria play an essential role in the disorder. Factors that regulate the mechanism of the mitochondrial quality control system have been drawing more and more attention. PGC-1α (peroxisome proliferator-activated receptor-γ coactivator-1α) is a powerful transcription factor involved in regulation of mitochondrial function. Glucagon-like peptide 1 (GLP-1), a brain-gut peptide, can enter the central nervous system through the blood-brain barrier and play neuroprotective role. However, whether the GLP-1R agonist liraglutide regulates mitochondrial quality control system through PGC-1α is still unclear. We administered different doses of liraglutide to intervene MPTP (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine)-induced PD model, and then immunofluorescence, Western blot, and stereotactic injection of lentivirus to downregulate PGC-1α were used to explore the mechanisms underlying the protective effect of liraglutide in PD. The results showed that MPTP lead to decreased mitochondrial biogenesis, disrupted mitochondrial dynamics, inhibited mitochondrial autophagy, and promoted cell apoptosis. While liraglutide effectively attenuated the neurotoxicity of MPTP, including reversing the dyskinesia caused by MPTP and preserving the expression of GLP-1R, TH, and PGC-1α in the substantia nigra (SN), further experiments showed that downregulation of PGC-1α expression via stereotactic injection PGC-1α lentivirus into the SN reversed the liraglutide protective effects. By PGC-1α downregulation, we found that PGC-1α can not only regulate mitochondria biogenesis, mitochondria dynamics, and autophagy, but also regulate cell apoptosis. In summary, liraglutide has a neuroprotective effect in the PD model induced by MPTP. This protective effect is accomplished by activating PGC-1α, which regulates the mitochondrial quality control system.

Neuroprotective effects of liraglutide against inflammation through the AMPK/NF-κB pathway in a mouse model of Parkinson's disease.

Parkinson's disease (PD) is a neurodegenerative disease with increasing incidence in aged populations, second only to Alzheimer's disease. Increasing evidence has shown that inflammation plays an important role in the occurrence and development of Parkinson's disease. Growing evidence has shown that AMP-activated protein kinase (AMPK) and NF-κB are closely related to inflammation. Glucagon-like peptide 1 (GLP-1) is a hormone that is primarily secreted by intestinal endocrine L cells, and it has a variety of physiology through binding to GLP-1 receptor. GLP-1can be used for treatment of type 2 diabetes. In addition, GLP-1 also has anti-neuroinflammation activity. However, the exact mechanism behind how GLP-1 regulates neuroinflammation remains unclear. This study was designed to examine the effect of liraglutide on 1-methyl-4-phenyl-1,2,3,6- tetrahydropyridine (MPTP)-induced injury in mice and its potential mechanism of action. Results showed that liraglutide dose-dependently ameliorated mouse behavior including swimming time and locomotor activity, increased the number of tyrosine hydroxylase (TH)-positive neurons and protein level, and reduced Iba1 and GFAP expression in the substantia nigra (SN). Liraglutide treatment also increased p-AMPK expression and reduced NF-κB protein level. Applying the AMPK inhibitor Dorsomorphin (Compound C) reversed the effect of liraglutide-reducing p-AMPK and increasing NF-κB expression. Finally, GFAP protein level increased, along with a decrease in TH expression. In conclusion, these results suggest that liraglutide can suppress neuroinflammation. Moreover, this effect is mediated through the AMPK/NF-κB signaling pathway.

Effect of Layer-Wise Varying Parameters on the Microstructure and Soundness of Selective Laser Melted INCONEL 718 Alloy.

Selective laser melting (SLM) is a promising powder bed fusion additive manufacturing technique for metal part fabrication. In this paper, varying scanning speed in the range of 500 mm/s to 1900 mm/s, and laser power in the range of 100 W to 200 W, were realized from layer to layer in a cycle of 56 layers in a single cuboid Inconel 718 alloy specimen through SLM. Layer-wise variation of microstructure and porosity were acquired, showing the layer-wise controlling capability of microstructural soundness. The melt pool size and soundness are closely linked with the energy input. High energy density led to sound regions with larger, orderly stacked melt pools and columnar grains, while low energy density resulted in porous regions with smaller, mismatched melt pools, un-melted powder, and equiaxed grains with finer dendrites. With the increase of laser energy density, the specimen shifts from porous region to sound region within several layers.

Effect of Scanning Routes on the Stress and Deformation of Overhang Structures Fabricated by SLM.

Selective laser melting (SLM) is a promising manufacturing method for the construction of complicated precision parts. However, deformation of the overhang during the fabrication process and post treatment is still a common problem. In this paper, the effect of the scanning route on the residual stress and deformation of fabricated AlSi10Mg overhang specimens by SLM was investigated. Different scanning routes for the overhang including longitudinal direction, transverse direction, and the alternation between these two scanning routes in consecutive layers were studied by experiments within this study. Numerical simulation was utilized to measure the stress of the specimens while deformation prediction was used for the different scanning routes. Both the experimental and simulated results showed that the scanning route had a substantial influence on the residual stress and deformation of the specimens. The longitudinal scanning resulted in significant upward bending deformation of the overhang as it was cut from the baseplate. However, there was less deformation for the overhangs fabricated by transverse and alternating scanning routes. A transverse scanning route is helpful for the reduction of residual stress in the longitudinal direction and the corresponding deformation.

Study on the Selective Laser Melting of CuSn10 Powder.

The selective laser melting of tin bronze (CuSn10) powder was performed with a laser energy density intensity level at 210, 220, and 230 J/mm². The composition was homogeneous with almost all tin dissolved into the matrix. The grain size of the obtained alpha copper phase was around 5 μm. The best properties were achieved at 220 J/mm² laser energy density with a density of 8.82 g/cm³, hardness of 78.2 HRB (Rockwell Hardness measured on the B scale), yield strength of 399 MPa, tensile strength of 490 MPa, and an elongation that reached 19%. “Balling effect” appeared and resulted into a decrease of properties when the laser energy density increased to 230 J/mm².

Visual and semi-quantitative analyses of dual-phase breast-specific gamma imaging with Tc-99m-sestamibi in detecting primary breast cancer.

OBJECTIVES: Breast cancer is the most common malignancy for females worldwide. This study was to evaluate the application of dual-phase breast-specific gamma imaging (BSGI) in detecting primary breast cancer. METHODS: Seventy-six patients with indeterminate breast lesions that underwent dual-phase BSGI enrolled in this study. All included lesions were confirmed by pathology. BSGI was evaluated based on the visual interpretation and dual-phase semi-quantitative indices of lesion to non-lesion ratio (L/N), which were compared with pathological results. The optimal visual analysis and L/N for double-phase were calculated through receiver operating characteristic curve analysis. RESULTS: Among 76 patients, 92 lesions were finally confirmed by the surgery and pathology, with 54 malignant and 38 benign lesions. Both early and delayed L/N of malignant breast diseases were significantly higher than those of benign (3.18±1.57 vs 1.53±0.59, and 2.91±1.91 vs 1.46±0.54, P<0.05). The optimal visual interpretation is over grade 3, and cut-off L/N was 2.06 and 1.77 for early and delayed imaging, respectively. Compared with visual analysis over grade 3 (77.8 and 81.6%), optimal early L/N (81.5 and 92.1%) or delayed L/N (79.5 and 89.5%) alone, the sensitivity and specificity of visual combined with early-phase L/N in diagnosing primary breast cancer are higher, which were 85.2 and 92.2%, respectively. CONCLUSIONS: The combination of visual and semi-quantitative analysis could improve the sensitivity and specificity of BSGI in detecting primary breast cancer. In addition, the potential value of delayed BSGI in diagnosing primary breast cancer should be further investigated in large samples.