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[This corrects the article DOI: 10.34133/2022/9873831.].
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The SARS-CoV-2 variants have been emerging and have made great challenges to current vaccine and pandemic control strategies. It is urgent to understand the current immune status of various Chinese populations given that the preexisting immunity has been established by national vaccination or exposure to past variants. Using sera from 85 individuals (including 21 convalescents of natural infection, 15 cases which suffered a breakthrough infection after being fully vaccinated, and 49 healthy vaccinees), we showed significantly enhanced neutralizing activities against SRAS-CoV-2 variants in convalescent sera, especially those who had been fully vaccinated. The neutralizing antibodies against Omicron were detectable in 75% of convalescents and 44.9% of healthy vaccinees (p = 0.006), with a GMT of 289.5, 180.9-463.3, and 42.6, 31.3-59, respectively. However, the neutralizing activities were weaker in young convalescents (aged < 18 y), with a detectable rate of 50% and a GMT of 46.4 against Omicron. We also examined and found no pan-sarbecovirus neutralizing activities in vaccinated SARS-CoV-1 survivors. A booster dose could further increase the breadth and magnitude of neutralization against WT and variants of concern (VOCs) to different degrees. In addition, we showed that COVID-19-inactivated vaccines can elicit Omicron-specific T-cell responses. The positive rates of ELISpot reactions were 26.7% (4/15) and 43.8% (7/16) in the full vaccination group and the booster vaccination group, respectively, although without statistically significant difference. The neutralizing antibody titers declined while T-cell responses remain consistent over 6 months. These findings will inform the optimization of public health vaccination and intervention strategies to protect diverse populations against SARS-CoV-2 variants. Advances. Breakthrough infection significantly boosted neutralizing activities against SARS-CoV-2 variants as compared to booster immunization with inactivated vaccine. Vaccine-induced virus-specific T-cell immunity, on the other hand, may compensate for the shortfall. Furthermore, the public health system should target the most vulnerable group due to a poorer protective serological response in both infected and vaccinated adolescents.
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Based on the changes in phase characteristics of blood flow and pressure, enhanced external counterpulsation (EECP) reduces cardiac load and improves cerebral perfusion in patients with cerebrovascular diseases. However, increased cerebral blood flow (CBF) is associated with the rise in blood pressure and its complications. Increased EECP frequency is a valuable solution when combined with the electrical equivalent impedance characteristics of the lumped parameter model (LPM) of the human blood circulation system. Herein, to investigate the effect of different EECP frequencies on CBF perfusion, an LPM was established with cardiopulmonary circulation and eight systemic blood flow units with cerebral autoregulation module of ischemic stroke patients. Then, using differential equations, we analyzed those parameters through hemodynamic simulations in four EECP modes. With related influencing parameters remaining constant, we adjusted the pressure frequency of EECP and found that when compared to the traditional sequential EECP mode, the relative increase rate of CBF was 16.68%, 18.95%, and 21.21% from 1 to 3 Hz, respectively. This study validates the effect of improving blood prefusion with increasing EECP frequency through numerical analysis.