Systematic Analysis of Autophagy-Related Signature Uncovers Prognostic Predictor for Acute Myeloid Leukemia.

Autophagy, a highly conserved cellular protein degradation process, has been involved in acute myeloid leukemia (AML). The present study aims to establish a novel, autophagy-related prognostic signature for prediction of AML prognosis. Differentially expressed autophagy-related genes in AML and healthy samples were screened using GSE1159. Univariate Cox regression analysis was applied to determine survival-associated autophagy-related genes in The Cancer Genome Atlas (TCGA) AML cohort. Lasso regression was performed to develop multiple-gene prognostic signatures. A novel six-gene signature (including CASP3, CHAF1B, KLHL24, OPTN, VEGFA, and VPS37C) DC was established for AML prognosis prediction. The Kaplan-Meier survival analysis revealed that patients in the high-risk score group had poorer overall survival (OS). The receiver operating characteristic (ROC) curve validated its good performance in survival prediction in TCGA AML cohort, and the area under the curve value was 0.817. Moreover, our signature could independently predict OS. A nomogram was constructed, including the six-gene signature and other clinical parameters, and predictive efficiency was confirmed using the ROC curve and calibration curve. Furthermore, gene set enrichment analyses identified several tumor-associated pathways that may contribute to explain the potential molecular mechanisms of our signature. Overall, we developed a new autophagy-associated gene signature and nomogram to predict OS of AML patients, which may help in clinical decision-making for AML treatment.

Di-µ-chlorido-bis-[chlorido(N,N'-dibenzyl-propane-1,2-diamine-κN,N')copper(II)].

In the title complex, [Cu(2)Cl(4)(C(17)H(22)N(2))(2)], the Cu(II) cation is coordinated by a N,N'-dibenzyl-propane-1,2-diamine ligand and two Cl(-) anions, and a Cl(-) anion from an adjacent mol-ecule further bridges to the Cu(II) cation in the apical position, with a longer Cu-Cl distance of 2.9858 (18) Å, forming a centrosymmetric dimeric complex in which each Cu(II) cation is in a distorted square-pyramidal geometry. Intra-molecular N-H⋯Cl hydrogen bonding is observed in the dimeric complex.

Diaqua-bis(N,N'-dibenzyl-ethane-1,2-diamine-κN,N')nickel(II) dichloride N,N-dimethyl-formamide solvate.

The asymmetric unit of the title complex, [Ni(C(16)H(20)N(2))(2)(H(2)O)(2)]Cl(2)·C(3)H(7)NO, consists of two Ni(II) atoms, each lying on an inversion center, two Cl anions, two N,N'-dibenzyl-ethane-1,2-diamine ligands, two coordinated water mol-ecules and one N,N-dimethyl-formamide solvent mol-ecule. Each Ni(II) atom is six-coordinated in a distorted octa-hedral coordination geometry, with the equatorial plane formed by four N atoms and the axial positions occupied by two water mol-ecules. The complex mol-ecules are linked into a chain along [001] by N-H⋯Cl, N-H⋯O and O-H⋯Cl hydrogen bonds. The C atoms and H atoms of the solvent mol-ecule are disordered over two sites in a ratio of 0.52 (2):0.48 (2).

Bis{6,6'-dimeth-oxy-2,2'-[ethane-1,2-diyl-bis(imino-methyl-ene)]diphenolato(1.5-)-κO,N,N',O'}erbium(III).

In the title compound, [Er(C(18)H(22.5)N(2)O(4))(2)], the Er atom is located on a twofold rotation axis and is eight-coordinated by four O atoms and four N atoms from two symmetry-related 6,6'-dimethoxy-2,2'-(ethane-1,2-diyldiiminodimethylene)diphenolate(1.5-) ligands. Due to disorder of one phenolate H atom with half-occupation, the overall charge of one tetradentate ligand is -1.5. The ligand molecules are stabilised by intramolecular N-H⋯O and O-H⋯O hydrogen bonds and are linked into a chain parallel to the a axis by a C-H⋯O hydrogen bond. Neighbouring chains are connected by van der Waals forces, resulting in a three-dimensional network.

Bis{6,6'-dimeth-oxy-2,2'-[ethane-1,2-diyl-bis(imino-methyl-ene)]diphenolato(1.5-)-κO,N,N',O'}praeseodymium(III).

The title compound, [Pr(C(18)H(22.5)N(2)O(4))(2)], is isotypic with its Er and Tb analogues. All interatomic distances, angles and the hydrogen bond geometry are very similar for the three structures..

Bis{6,6'-dimeth-oxy-2,2'-[ethane-1,2-diyl-bis(imino-methyl-ene)]diphenolato(1.5-)-κO,N,N',O'}terbium(III).

The title compound, [Tb(C(18)H(22.5)N(2)O(4))(2)], is isotypic with its Pr and Tb analogues. All interatomic distances, angles and the hydrogen bond geometry are very similar for the three structures.

Tetra-kis(µ-naphthalene-1-acetato-1:2κO:O')bis-(naphthalene-1-acetato)-1κO,O';2κO,O'-bis-(1,10-phenanthroline)-1κN,N';2κN,N'-europium(III)samarium(III).

In the title compound, [EuSm(C(12)H(9)O(2))(6)(C(12)H(8)N(2))(2)], the metal site is statistically occupied (50:50) by Eu and Sm atoms, forming a centrosymmetric complex. The metal site is nine-coordinate, in a distorted monocapped square-anti-prismatic coordination geometry. Mol-ecules are linked into three chains by C-H⋯π interactions and C-H⋯O hydrogen bonds. The combination of these chains generates a three-dimensional framework structure. One of the bridging naphthalene-1-ace-tate ligands was found to be disordered over two sites; the site occupancies for the naphthylmethyl group refined to 0.628 (14) and 0.372 (14).

Poly[(N,N-dimethyl-formamide-κO)tris-(µ-naphthalene-1-acetato)terbium(III)].

In title compound, [Tb(C(12)H(9)O(2))(3)(C(3)H(7)NO)](n), the Tb atom is nine-coordinated by nine O atoms from three naphthalene-1-acetate and one N,N-dimethyl-formamide ligands. The Tb atoms are linked by three bridging naphthalene-1-acetate ligands into a chain parallel to the b axis. Further stabilization of the structure is accomplished by non-classical C-H⋯O hydrogen bonds and C-H⋯π interactions.

The dinuclear copper(II) complexes di-mu-chlorido-bis{[N,N'-bis(4-chlorobenzyl)propane-1,2-diamine]chloridocopper(II)} and di-mu-chlorido-bis{[N,N'-bis(3,4-methylenedioxybenzyl)propane-1,2-diamine]chloridocopper(II)}.

The two title dinuclear copper(II) complexes, [Cu2Cl4(C17H20Cl2N2)2], (I), and [Cu2Cl4(C19H22N2O4)2], (II), have similar coordination environments. In each complex, the asymmetric unit consists of one half-molecule and the two copper centres are bridged by a pair of Cl atoms, resulting in complexes with centrosymmetric structures containing Cu(mu-Cl)2Cu parallelogram cores; the Cu...Cu separations and Cu-Cl-Cu angles are 3.4285 (8) A and 83.36 (3) degrees, respectively, for (I), and 3.565 (2) A and 84.39 (7) degrees for (II). Each Cu atom is five-coordinated and the coordination geometry around the Cu atom is best described as a distorted square-pyramid with a tau value of 0.155 (3) for (I) and 0.092 (7) for (II). The apical Cu-Cl bond length is 2.852 (1) A for (I) and 2.971 (2) A for (II). The basal Cu-Cl and Cu-N average bonds lengths are 2.2673 (9) and 2.030 (2) A, respectively, for (I), and 2.280 (2) and 2.038 (6) A for (II). The molecules of (I) are linked by one C-H...Cl hydrogen bond into a complex [10 1] sheet. The molecules of (II) are linked by one C-H...Cl and one N-H...O hydrogen bond into a complex [100] sheet.

[N,N'-Bis(4-chloro-benz-yl)ethane-1,2-diamine]dichloridozinc(II).

In the title complex, [ZnCl(2)(C(16)H(18)Cl(2)N(2))], the asymmetric unit contains one mol-ecule and two half-mol-ecules, which have similar geometric parameters; in the latter two molecules each Zn atom lies on a twofold rotation axis. The environment about each Zn(II) atom is distorted tetra-hedral with coordination of two terminal Cl atoms and two N atoms of the N,N'-bis-(4-chloro-benz-yl)ethane-1,2-diamine ligand. Four N-H⋯Cl hydrogen bonds link the mol-ecules into a chain of R(2) (2)(8) rings in the [001] direction.