High-performance bismuth vanadate photoanodes cocatalyzed with nitrogen, sulphur co-doped ferrocobalt-metal organic frameworks thin layer for photoelectrochemical water splitting.

In this study, we prepare a highly efficient BiVO4 photoanode co-catalyzed with an ultrathin layer of N, S co-doped FeCo-Metal Organic Frameworks (MOFs) for photoelectrochemical water splitting. The introduction of N and S into FeCo-MOFs enhances electron and mass transfer, exposing more catalytic active sites and significantly improving the catalytic performance of N, S co-doped FeCo-based MOFs in water oxidation. The optimized BiVO4/NS-FeCo-MOFs photoanode exhibits impressive results, with a photocurrent density of 5.23 mA cm-2 at 1.23 V vs. Reversible Hydrogen Electrode (RHE) and an incident photon-to-charge conversion efficiency (IPCE) of 74.4 % at 450 nm in a 0.1 M phosphate buffered solution (pH = 7). These values are 4.84 times and 6.2 times higher than those of the original BiVO4 photoanode, respectively. Furthermore, the optimized BiVO4/NS-FeCo-MOFs photoanode demonstrates exceptional long-term stability, maintaining 96 % of the initial current after five hours.

Flower ball cathode assembled from Cu doped Co3S4/Ni3S2 ultrathin nanosheets in a photocatalytic fuel cell for efficient photoelectrochemical rifampicin purification and simultaneous electricity generation based on a CuO QDs/TiO2/WO3 photoanode.

Herein, an efficient CuO QDs/TiO2/WO3 photoanode and a Cu doped Co3S4/Ni3S2 cathode were successfully synthesized. The optimized CuO QDs/TiO2/WO3 photoanode achieved a photocurrent density of 1.93 mA cm-2 at 1.23 vs. RHE, which was 2.27 times that of a WO3 photoanode. The CuO QDs/TiO2/WO3-buried junction silicon (BJS) photoanode was coupled with the Cu doped Co3S4/Ni3S2 cathode to construct a novel photocatalytic fuel cell (PFC) system. The as-established PFC system showed a high rifampicin (RFP) removal ratio of 93.4% after 90 min and maximum power output of 0.50 mW cm-2. Quenching tests and EPR spectra demonstrated that ˙OH, ˙O2- and 1O2 were the main reactive oxygen species in the system. This work provides a possibility to construct a more efficient PFC system for environmental protection and energy recovery in the future.

Expression Profiles, Prognosis, and ceRNA Regulation of SRY-Related HMG-Box Genes in Stomach Adenocarcinoma.

Aberrant expression of the SRY-related HMG-box (SOX) genes contributes to tumor development and progression. This research aimed to identify the regulation of the SOX genes in stomach adenocarcinoma (STAD). Expression profiles downloaded from The Cancer Genome Atlas (TCGA) were conducted to analyze the expression and function of the SOX genes. A competing endogenous RNAs (ceRNA) network mediated by the SOX genes was effectively constructed consisting of 64 lncRNAs, 29 miRNAs, and 11 SOX genes based on predicted miRNAs shared by lncRNAs and mRNAs using miRDB, TargetScan, miRTarBase, miRcode, and starBase v2.0. SOX9 was identified as a prognostic signature, which showed the usefulness of diagnosis and prognosis of STAD by the receiver operating characteristic (ROC) and Kaplan-Meier curves. SOX9 was also shown specifically in STAD and identified as highly expressed in the gastrointestinal tract. Gene Ontology (GO) enrichment analysis showed that SOX9 might influence the genes related to the pattern specification process, sodium ion homeostasis, and potassium ion transport, mainly including FEZF1, HOXC13, HOXC10, HOXC9, HOXA11, DPP6, ATP4B, CASQ2, KCNA1, ATP4A, and SFRP1. Furthermore, HOTAIR knockdown, miR-206-mimic transfection, the Cell Count Kit-8 (CCK-8) assay were performed to verify the function of HOTAIR/miR-206/SOX9 axis, which was identified in the ceRNA network analysis. HOTAIR could induce proliferation potentially by competitively binding miR-206/SOX9 axis in STAD. These findings provide new clues with prognostic and therapeutic implications in STAD and suggest that HOTAIR/miR-206/SOX9 might be a potential new strategy for therapeutic targeting of gastric cancer.

Distinct Urinary Metabolic Biomarkers of Human Colorectal Cancer.

Colorectal cancer (CRC) is one of the most commonly diagnosed cancers with high mortality rate due to its poor diagnosis in the early stage. Here, we report a urinary metabolomic study on a cohort of CRC patients (n =67) and healthy controls (n =21) using ultraperformance liquid chromatography triple quadrupole mass spectrometry. Pathway analysis showed that a series of pathways that belong to amino acid metabolism, carbohydrate metabolism, and lipid metabolism were dysregulated, for instance the glycine, serine and threonine metabolism, alanine, aspartate and glutamate metabolism, glyoxylate and dicarboxylate metabolism, glycolysis, and TCA cycle. A total of 48 differential metabolites were identified in CRC compared to controls. A panel of 12 biomarkers composed of chenodeoxycholic acid, vanillic acid, adenosine monophosphate, glycolic acid, histidine, azelaic acid, hydroxypropionic acid, glycine, 3,4-dihydroxymandelic acid, 4-hydroxybenzoic acid, oxoglutaric acid, and homocitrulline were identified by Random Forest (RF), Support Vector Machine (SVM), and Boruta analysis classification model and validated by Gradient Boosting (GB), Logistic Regression (LR), and Random Forest diagnostic model, which were able to discriminate CRC subjects from healthy controls. These urinary metabolic biomarkers provided a novel and promising molecular approach for the early diagnosis of CRC.

Biomimetic Nanocarriers Guide Extracellular ATP Homeostasis to Remodel Energy Metabolism for Activating Innate and Adaptive Immunity System.

Metabolic interventions via targeting intratumoral dysregulated metabolism pathways have shown promise in reinvigorating antitumor immunity. However, approved small molecule immunomodulators often suffer from ineffective response rates and severe off-target toxicity. ATP occupies a crucial role in energy metabolism of components that form the tumor microenvironment (TME) and influences cancer immunosurveillance. Here, a nanocarrier-assisted immunometabolic therapy strategy that targets the ATP-adenosine axis for metabolic reprogramming of TME is reported. An ecto-enzyme (CD39) antagonist POM1 and AMP-activated protein kinase (AMPK) agonist metformin are both encapsulated into cancer cell-derived exosomes and used as nanocarriers for tumor targeting delivery. This method increases the level of pro-inflammatory extracellular ATP (eATP) while preventing the accumulation of immunosuppressive adenosine and alleviating hypoxia. Elevated eATP triggers the activation of P2X7-NLRP3-inflammasome to drive macrophage pyroptosis, potentiates the maturation and antigen capacity of dendritic cells (DCs) to enhance the cytotoxic function of T cells and natural killer (NK) cells. As a result, synergistic antitumor immune responses are initiated to suppress tumor progress, inhibit tumor distant metastases, provide long-term immune memory that offers protection against tumor recurrence and overcome anti-PD1 resistance. Overall, this study provides an innovative strategy to advance eATP-driven antitumor immunity in cancer therapy.

High-performance BiVO4 photoanodes cocatalyzed with bilayer metal-organic frameworks for photoelectrochemical application.

In this work, we modified a BiVO4 photoanode with bilayer Fe-MOF and Ni-MOF as cocatalysts for the first time and obtained a highly efficient BiVO4 composite photoanode whose photocurrent density was increased by 2.7 times. The optimized BiVO4/Fe-MOF/Ni-MOF photoanode demonstrated a photocurrent density of 1.80 mA/cm2 at 1.23 V vs. a reversible hydrogen electrode (RHE). The onset potential of the BiVO4/Fe-MOF/Ni-MOF photoanode markedly decreased from 0.9 V to 0.69 V in comparison with the pure BiVO4 photoanode. It is speculated that Fe-MOF and Ni-MOF led to more reactive oxygen evolution sites and that the bilayer cocatalysts synergistically promoted the separation of photogenerated electron-hole pairs, which may be the influencing factor for the photoelectrochemical performance of the BiVO4/Fe-MOF/Ni-MOF photoanode being distinctively enhanced. Thus, this work sheds some interesting new light on the construction of a high-efficiency photoanode for photoelectrochemical applications.

Tumor-derived exosomes co-delivering aggregation-induced emission luminogens and proton pump inhibitors for tumor glutamine starvation therapy and enhanced type-I photodynamic therapy.

Although promising, the efficiency of aggregation-induced emission luminogens (AIEgens)-based photodynamic therapy (PDT) is limited by cellular glutathione (GSH). GSH is not a terminal reducing agent but it can be oxidized and subsequently reduced to its original state by reductases to further participate in antioxidant activity. It is therefore imperative to control GSH for effectively inducing oxidation within tumor cells. Recent studies showed that tumor cell metabolism depends mainly on glutamine, which is also the nitrogen and ATP source for GSH synthesis. Therefore, glutamine-based starvation therapy may be effective in enhancing photodynamic therapy. In this work, tumor-derived exosomes were developed for co-delivering AIEgens and proton pump inhibitors (PPI) for tumor combination therapy. Tumor-derived exosomes could specifically deliver drugs to the tumor sites, where PPI inhibited cell glutamine metabolism, suppressed tumor cell GSH and ATP production, and improved the effect of type-I PDT from AIEgens. When used in the treatment of MGC803 gastric cancer subcutaneous model, our system shows a high tumor growth inhibition rate, and even promoting tumor immunogenic death. This is the first work which combine inhibition of glutamine metabolism with PDT, and it has the potential to be applied for future designs of new tumor metabolic therapies and photodynamic systems.

Huge gastric plexiform fibromyxoma presenting as pyemia by rupture of tumor: A case report.

BACKGROUND: Plexiform fibromyxoma (PF) is a rare mesenchymal tumor, with limited case reports worldwide. Common clinical symptoms are abdominal discomfort and bleeding signs, which frequently present slow-onset in reported cases. Herein, we report a case of gastric PF presenting as acute onset and with pyemia accom-panying tumor rupture. We resected the tumor as well as the distal gastric, bulbus duodeni and gallbladder for treatment in emergency surgery. Notably, before the onset of the disease, the patient received coronavirus disease 2019 (COVID-19) vaccines. CASE SUMMARY: A 26-year-old man was admitted to our hospital, due to abdominal pain and fever after having received COVID-19 vaccines. Laboratory examination indicated severe sepsis. Computed tomography scan revealed a large mass in the abdomen. Deformation of the gastrointestinal tract was seen during gastroscopy. After failure of anti-infective treatment and symptoms of shock developed, he received an emergency surgery. We found a huge and partly ruptured mass, with thick purulence. Microscopically, the mass was composed of spindle cells with clarified cytoplasm, accompanied by myxoid stroma and arborizing blood vessels. Immunohistochemistry showed the tumor cells as positive for smooth muscle actin and succinate dehydrogenase subunit B but negative for DOG-1 and CD117. Finally, the patient was diagnosed with gastric PF and discharged from the hospital. CONCLUSION: Gastric PF manifesting as tumor rupture combined with pyemia is rare. Timely surgery is critical for optimal prognosis.

Enhanced reactive oxygen species via in situ producing H2O2 and synchronous catalytic conversion at stable modified copper foam cathode for efficient high-concentration organic wastewater treatment and simultaneous electricity generation.

Efficient high-concentration organics degradation (including 2-CP, phenol, and tetracycline) and simultaneous electricity generation were achieved via in situ producing H2O2 and synchronous catalytic conversion to more reactive oxygen species at stable modified copper foam cathode. The cathode was synthesized using the one-pot electrodeposition method and was used to in-situ generate H2O2 through the two-electron reduction of oxygen. The produced H2O2 was then catalytically converted into ·OH and ·O2- simultaneously. The results showed that the system using the Au-Fe co-modified cathode achieved an optimal rhodamine b (50 mg L-1) removal ratio and the removal ratios of 2-CP, phenol and tetracycline were all higher than 90% in 120 min. Meanwhile, it exhibited a high conversion performance of organics into electricity, which is superior to most of the reported PFC (Photocatalytic Fuel Cell) systems. Electron spin resonance test was conducted to ascertain the role of ·O2- and ·OH in the organics degradation. Furthermore, the Au-Fe-modified cathode exhibited superior stability for long-term application in the pH range of 3-7, which can be attributed to the protection of photocurrent and the interaction between Cu and Fe.

Integrative multiplatform-based molecular profiling of human colorectal cancer reveals proteogenomic alterations underlying mitochondrial inactivation.

Mitochondria play leading roles in initiation and progression of colorectal cancer (CRC). Proteogenomic analyses of mitochondria of CRC tumor cells would likely enhance our understanding of CRC pathogenesis and reveal new independent prognostic factors and treatment targets. However, comprehensive investigations focused on mitochondria of CRC patients are lacking. Here, we investigated global profiles of structural variants, DNA methylation, chromatin accessibility, transcriptome, proteome, and phosphoproteome on human CRC. Proteomic investigations uncovered greatly diminished mitochondrial proteome size in CRC relative to that found in adjacent healthy tissues. Integrated with analysis of RNA-Seq datasets obtained from the public database containing mRNA data of 538 CRC patients, the proteomic analysis indicated that proteins encoded by 45.5% of identified prognostic CRC genes were located within mitochondria, highlighting the association between altered mitochondrial function and CRC. Subsequently, we compared structural variants, DNA methylation, and chromatin accessibility of differentially expressed genes and found that chromatin accessibility was an important factor underlying mitochondrial gene expression. Furthermore, phosphoproteomic profiling demonstrated decreased phosphorylation of most mitochondria-related kinases within CRC versus adjacent healthy tissues, while also highlighting MKK3/p38 as an essential mitochondrial regulatory pathway. Meanwhile, systems-based analyses revealed identities of key kinases, transcriptional factors, and their interconnections. This research uncovered a close relationship between mitochondrial dysfunction and poor CRC prognosis, improve our understanding of molecular mechanism underlying mitochondrial linked to human CRC, and facilitate identifies of clinically relevant CRC prognostic factors and drug targets.

Multi-omics analyses of human colorectal cancer revealed three mitochondrial genes potentially associated with poor outcomes of patients.

BACKGROUND: The identification of novel functional biomarkers is essential for recognizing high-risk patients, predicting recurrence, and searching for appropriate treatment. However, no prognostic biomarker has been applied for colorectal cancer (CRC) in the clinic. METHODS: Integrated with transcriptomic data from public databases, multi-omics examinations were conducted to search prognostic biomarkers for CRC. Moreover, the potential biological functions and regulatory mechanism of these predictive genes were also explored. RESULTS: In this study, we revealed that three mitochondrial genes were associated with the poor prognosis of CRC. Integrated analyses of transcriptome and proteome of CRC patients disclosed numerous down-regulated mitochondrial genes at both mRNA and protein levels, suggesting a vital role of mitochondria in carcinogenesis. Combined with the bioinformatics studies of transcriptomic datasets of 538 CRC patients, three mitochondrial prognostic genes were eventually selected out, including HIGD1A, SUCLG2, and SLC25A24. The expression of HIGD1A exhibited a significant reduction in two subtypes of adenoma and six subtypes of CRC, while the down-regulation of SUCLG2 and SLC25A24 showed more advantages in rectal mucinous adenocarcinoma. Moreover, we unveiled that these three genes had common expressions and might collaboratively participate in the synthesis of ribosomes. Our original multi-omics datasets, including DNA methylation, structural variants, chromatin accessibility, and phosphoproteome, further depicted the altered modifications on their potential transcriptional factors. CONCLUSIONS: In summary, HIGD1A, SUCLG2, and SLC25A24 might serve as predictive biomarkers for CRC. The biological activities they involved in and their upstream regulators we uncovered would provide a functional context for the further-in-depth mechanism study.

Delivery of manganese carbonyl to the tumor microenvironment using Tumor-Derived exosomes for cancer gas therapy and low dose radiotherapy.

The development of novel radiosensitizer with high selectivity and controllability is highly desirable. CO gas could cause damage to mitochondria and thus enhance RT effect. Controlled delivery of CO in tumor is important both to achieve high-efficiency of CO gas therapy and to decrease the risk of CO poisoning. In this study, manganese carbonyl (MnCO) loaded exosome nano-vesicles (MMV) to overcome this conundrum for tumor therapy is developed. After administration, MMV showed its admirable performance in active tumor-targeting, mitochondria damage and radiosensitization therapy. These MMV nanoparticles were able to facilitate robust CO evolution and consequent ROS generation in response to X-ray irradiation both in vitro and in vivo. Significantly, MMV could facilitate a 90% inhibition effect of tumor growth under very low dose (only 2Gy) RT, which is better than high dose (6Gy) radiotherapy. Overall, this study highlights a novel and practical approach to enhancing the efficacy of tumor RT, underscoring the value of future research in the field of CO medicine.

Modification of Black Phosphorus Nanosheets with a Ni-Containing Carbon Layer as Efficient and Stable Hydrogen Production Electrocatalysts.

Few-layered black phosphorus (FP) has recently attracted extensive research in the energy and materials fields. However, because of its chemically unstable nature under ambient conditions, very positive hydrogen adsorption energy and less active sites, FP has not been an efficient catalyst for the hydrogen evolution reaction (HER). In this research, we have developed a new strategy to overcome FP's drawbacks and to make it an active and stable HER catalyst. Our approach is to deposit a Ni2+-anchored thin carbon layer onto the surface of FP via controlled decarboxylation of Ni ethylenediaminetetraacetate (Ni-EDTA). The carbon layer on the surface of FP prevents it from making direct contact with its external environment, thereby greatly improving its stability. At the same time, transition-metal Ni that is dispersed in its carbon layer changes its hydrogen adsorption energy so as to improve its electrocatalytic activity. The prepared FP@Ni-C shows an outstanding HER performance with an overpotential of only 284 mV to obtain 10 mA cm-2 current density with excellent electrocatalytic stability. The FP@Ni-C catalyst showed almost no activity loss during a 12 h catalyst life test. This study provides a new approach to the synthesis of highly efficient and stable electrocatalysts based on two-dimensional materials, using a facile catalyst preparation method.

Nano-Platelets as an Oxygen Regulator for Augmenting Starvation Therapy Against Hypoxic Tumor.

The restriction of a tumor's energy supply is proven to be an effective means of treatment. Glucose oxidase (GOx), an enzyme that catalyzes the conversion of glucose to glucolactone, producing oxygen and hydrogen peroxide in the process, has proved useful in this regard. However, hypoxia, which is implicated in tumor growth, has been found to mediate resistance to this type of tumor starvation. Here, we describe the design and testing of a platelet membrane mimetic, PMS, consisting of mesoporous silica nanoparticles (MSNs) loaded with metformin (MET) as an inner layer and platelet membranes (PM) as an outer layer that inhibits oxygen consumption by the tumor cells' respiratory pathways and enhances the effectiveness of GOx. MET directly inhibits the activity of complex I in mitochondrial electron transport and is thus a potent inhibitor of cell respiration. PMS target tumor tissue effectively and, once internalized, MET can inhibit respiration. When oxygen is plentiful, GOx promotes glucose consumption, allowing amplification of its effects on tumor starvation. This combination of respiratory suppression by PMS and starvation therapy by GOx has been found to be effective in both targeting tumors and inhibiting their growth. It is hoped that this strategy will shed light on the development of next-generation tumor starvation treatments.

Label-free and self-assembled fluorescent DNA nanopompom for determination of miRNA-21.

A label-free fluorescence method based on self-assembled DNA nanopompom has been developed for miRNA-21 detection. In the presence of miRNA-21, three DNA hairpin probes with split G-quadruplex assemble the DNA nanopompom. Based on the isothermal toehold-mediated DNA strand displacement reaction, the target miRNA can be catalytically recycled and trigger three DNA hairpin probes to self-assemble the DNA nanopompom and release the G-quadruplex. The formation of the G-quadruplex increases the fluorescence emission intensity of thioflavin. For thioflavin-based miRNA-21 detection, the excitation and emission wavelengths are set to 425 nm and 490 nm, respectively. The limit of detection for miRNA-21 is 0.8 pM according to F/F0 = 0.0031 × CmiRNA-21 + 1.0382 (R2 = 0.9978). This sensing system provides a low-cost, effective, and convenient method for miRNA detection, which holds great potential in biochemical diagnosis and clinical practice. Graphical abstract Label-free and self-assembled fluorescent DNA nanopompom for miRNA detection.

Tumor-Exocytosed Exosome/Aggregation-Induced Emission Luminogen Hybrid Nanovesicles Facilitate Efficient Tumor Penetration and Photodynamic Therapy.

The development of novel photosensitizing agents with aggregation-induced emission (AIE) properties has fueled significant advances in the field of photodynamic therapy (PDT). An electroporation method was used to prepare tumor-exocytosed exosome/AIE luminogen (AIEgen) hybrid nanovesicles (DES) that could facilitate efficient tumor penetration. Dexamethasone was then used to normalize vascular function within the tumor microenvironment (TME) to reduce local hypoxia, thereby significantly enhancing the PDT efficacy of DES nanovesicles, and allowing them to effectively inhibit tumor growth. The hybridization of AIEgen and biological tumor-exocytosed exosomes was achieved for the first time, and combined with PDT approaches by normalizing the intratumoral vasculature as a means of reducing local tissue hypoxia. This work highlights a new approach to the design of AIEgen-based PDT systems and underscores the potential clinical value of AIEgens.

A lysosome specific, acidic-pH activated, near-infrared Bodipy fluorescent probe for noninvasive, long-term, in vivo tumor imaging.

Long-term, in vivo, fluorescent cell tracking probes are useful for understanding complex cellular processes including tissue regeneration, communication, development, invasion, and cancer metastasis. A near-infrared fluorescent, water-soluble probe is particularly important for studying these biological events and processes. Herein, a lysosome specific, near-infrared Bodipy probe with increased fluorescent intensity in the acidic, lysosome environment is reported. This Bodipy probe is packaged in a nanoparticle using DSPE-PEG2000. The resulting nanoparticle is intravenously delivered to a tumor xenograft, where the fluorescent Bodipy becomes useful for non-invasive, long-term, in vivo fluorescent tumor imaging for periods greater than 36 days. These long-term, in vitro and in vitro tracking data indicate that the described Bodipy nanoparticles hold great potential for monitoring biological processes.

Efficient organic pollutants conversion and electricity generation for carbonate-containing wastewater based on carbonate radical reactions initiated by BiVO4-Au/PVC system.

In this paper, we propose an efficient simultaneous refractory organics degradation and electricity generation method for carbonate-containing wastewater based on carbonate radical reactions initiated by a BiVO4-Au/PVC (PVC: photovoltaic cell) system. In the system, nanoporous BiVO4 film and Au modified PVC were used as photoanode and photocathode, respectively. HCO3- was used as the electrolyte. Carbonate radicals, which have lower recombination rates than hydroxyl radicals and strong oxidation abilities, can be generated easily by the capture reaction of hydroxyl radicals with HCO3-, which is one of the most abundant anions in the aquatic environment. The results show that the removal ratios of rhodamine b, methyl orange and methylene blue in the system increased sharply to 77.98 %, 89.15 % and 93.2 % from 18.23 %, 21 % and 23.14 % (BiVO4-Pt/ SO42-), respectively, after 120 min. Meanwhile, the short-circuit current density is up to 2.19-2.41 times larger than the traditional system. Other common ions in natural water minimally affected the properties of the new system. The excellent performance could be ascribed to large amounts of carbonate radicals in the system, which have great potential for efficient carbonate-containing wastewater treatment and energy recovery.

Analysis of autophagy-related genes and associated noncoding RNAs and transcription factors in digestive system tumors.

Aim: To investigate the autophagy-related gene (ATG) expression and the associated noncoding RNAs (ncRNA) and transcription factors (TF) in digestive system tumors (DST). Methods: We systematically investigated the ATG expression in DST by weighted gene correlation network analysis, crosstalk connection, functional analysis and Pivot analysis. Results: ATGs were clustered into six modules with co-expression in DST. Functional analysis revealed that six ATG-related modules were enriched in biological pathways involved in tumorigenesis. Pivot analysis identified key ncRNAs and TFs, which are essential for the pathogenesis, clinical diagnosis and treatment of DST. Conclusion: Our study highlights the crucial roles of ncRNA and TFs in the identification of potential biomarkers or therapeutic targets for DST.

Local staging of rectal cancer using fused high resolution diffusion weighted imaging and modified MR rectography.

Rectal cancer (RC) is a common malignant tumor with high mortality. MR imaging plays an important role in treatment decision making of RC. Unfortunately, the contents (gas and feces) in the rectum often induce artifacts and thus negatively affect the depicting and staging of RC. We developed a new protocol for MR rectography using oral administration of iso-osmotic mannitol to distend lumen after bowel cleansing preparation. Fused MR rectography and high resolution diffusion weighted imaging (DWI) is then performed to facilitate detection and staging of RC. Our present technique can eliminate the effect of gas and feces on image quality, especially on DWI, and can achieve satisfactory bowel distention, lesion depicture and visualization of surgical planes. Fused high resolution DWI and MR rectography can be a promising technique to improve the accuracy of RC local staging.