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Cell Res ; 32(12): 1068-1085, 2022 12.
Artigo em Inglês | MEDLINE | ID: mdl-36357786

RESUMO

The emerging SARS-CoV-2 variants, commonly with many mutations in S1 subunit of spike (S) protein are weakening the efficacy of the current vaccines and antibody therapeutics. This calls for the variant-proof SARS-CoV-2 vaccines targeting the more conserved regions in S protein. Here, we designed a recombinant subunit vaccine, HR121, targeting the conserved HR1 domain in S2 subunit of S protein. HR121 consisting of HR1-linker1-HR2-linker2-HR1, is conformationally and functionally analogous to the HR1 domain present in the fusion intermediate conformation of S2 subunit. Immunization with HR121 in rabbits and rhesus macaques elicited highly potent cross-neutralizing antibodies against SARS-CoV-2 and its variants, particularly Omicron sublineages. Vaccination with HR121 achieved near-full protections against prototype SARS-CoV-2 infection in hACE2 transgenic mice, Syrian golden hamsters and rhesus macaques, and effective protection against Omicron BA.2 infection in Syrian golden hamsters. This study demonstrates that HR121 is a promising candidate of variant-proof SARS-CoV-2 vaccine with a novel conserved target in the S2 subunit for application against current and future SARS-CoV-2 variants.


Assuntos
Vacinas contra COVID-19 , COVID-19 , Animais , Cricetinae , Camundongos , Humanos , Coelhos , SARS-CoV-2 , Macaca mulatta , Mesocricetus , Glicoproteína da Espícula de Coronavírus/genética , COVID-19/prevenção & controle , Anticorpos Neutralizantes , Camundongos Transgênicos , Anticorpos Antivirais
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