RESUMO
Long noncoding RNA, RNA component of mitochondrial RNA processing endoribonuclease (RMRP) plays an important role in cancer development and is closely correlated with prognosis in cancer patients. However, whether RMRP affects prognosis in patients with hepatocellular carcinoma (HCC) remains unclear. The aim of the present study was to investigate the expression level of RMRP in HCC and its correlation with prognosis in patients with HCC and explain the effects and associated mechanisms by conducting an in vitro study. The high expression level of RMRP was correlated with poor prognosis in patients with HCC. Using in vitro analysis, RMRP knockdown suppressed HCC cell proliferation, invasion, and migration (P < .05). miRNA-206 overexpression had similar effects in HCC cell lines (Bel-7402 and Huh-7). Using Western blot analysis and cellular immunofluorescence detection, RMRP downregulation significantly suppressed TACR1/Erk1/2 pathway, while miRNA-206 was significantly upregulated (P < .05). RMRP downregulation inhibits HCC-related biological activities by the regulation of miRNA-206/TACR1.
Assuntos
Carcinoma Hepatocelular , Técnicas de Silenciamento de Genes , Neoplasias Hepáticas , MicroRNAs , Proteínas de Neoplasias , RNA Longo não Codificante/genética , RNA Neoplásico , Receptores da Neurocinina-1 , Carcinoma Hepatocelular/genética , Carcinoma Hepatocelular/metabolismo , Carcinoma Hepatocelular/patologia , Células Hep G2 , Humanos , Neoplasias Hepáticas/genética , Neoplasias Hepáticas/metabolismo , MicroRNAs/genética , MicroRNAs/metabolismo , Proteínas de Neoplasias/genética , Proteínas de Neoplasias/metabolismo , RNA Longo não Codificante/metabolismo , RNA Neoplásico/genética , RNA Neoplásico/metabolismo , Receptores da Neurocinina-1/genética , Receptores da Neurocinina-1/metabolismoRESUMO
A novel kind of optrode fabricated on a sapphire substrate is proposed for optogenetic applications in neuroscience. Eight thin-film neural electrodes and a GaN-LED are monolithically integrated on the surface of a sapphire shank. The LED is used for optogenetic stimulation and the multiple electrodes are used for simultaneous recording of neural activities. The output power density of the LED is 1-19 mW/mm2 at 468 nm, driving with a current from 0.7-10 mA. The mean electrochemical impedance of the eight recoding sites on the optrode at 1 kHz is 385 kΩ. The highest temperature-raise at tissue around the LED is almost 1 °C when the output power density is 3 mw/mm2. The monolithic integrated structure will make it a powerful tool for optogenetics.
Assuntos
Óxido de Alumínio , Optogenética/instrumentação , Optogenética/métodos , Óxido de Alumínio/química , Animais , Encéfalo , Impedância Elétrica , Eletrodos , Desenho de Equipamento , TemperaturaRESUMO
The size-controllable and highly monodispersed cuboidal barium strontium titanate (BST) nanocrystals have been successfully synthesized through a glycothermal process with no mineralizers but oleic acid as growth-directing agent. The synthesized BST nanocrystals under different glycothermal conditions were structurally characterized by XRD, IR, FESEM, TEM and HRTEM and investigated with respect to the effects of key influencing factors including the amount of oleic acid, duration of glycothermal process and 1,4-butanediol/water volume ratio in the reaction media on the formation of BST nanocrystals and their size and morphological evolution. It has been found that the oleic acid incorporated into the glycothermal system plays a decisive role in promoting the formation of cuboidal nanocrystals. It allows the BST nanocrystals to form via a nucleation-growth mechanism instead of in situ reactions and the selective chemical adsorption of oleic acid molecules on the facets with lower plane indices of newly-built BST nuclei directs them to grow into uniform cuboidal BST nanocrystals. The duration of glycothermal reactions and the polarity of reaction media can remarkably affect the dynamic process of the formation of BST nanocrystals. These regularly-shaped and highly monodispersed nanocrystals show a spontaneity of self-assembling into 2D ordered architectures when they were dispersed in organic solvents like cyclohexane and droped onto a hydrophobic surface of substrates, which creates a chance for the ferroelectric oxide nanocrystals to self-assemble into nanoscale electronic devices.