The zinc absorption of the novel peptide-Zn complex in Caco-2 cells: effects of soybean peptides charge and hydrophobicity.

BACKGROUND: The supplemental effect of zinc depends not only on adequate intake, but also on how efficiently it is absorbed in the small intestine. In the present study, weak hydrophobic peptides (WHP), strong hydrophobic peptides (SHP), positively charged peptides (PCP) and negatively charged peptides (NCP) were isolated from soybean peptides (SP). The peptide-Zn complexes (PCP-Zn, NCP-Zn, WHP-Zn, SHP-Zn and SP-Zn) were prepared to compare their promotion zinc absorption capacity in the Caco-2 cells monolayers model. RESULTS: We found that the carboxyl, carbonyl and amino groups in peptide were the primary binding sites of Zn. Compared with zinc sulfate, the peptide-Zn complexes with different charge and hydrophobic peptides could improve zinc solubility at different pH. NCP-Zn had a lower Zn-binding capacity but a higher zinc absorption capacity compared to that of PCP-Zn in Caco-2 cells. In addition, the capacity of PCP-Zn to promote zinc absorption was lower than the control group (SP-Zn). There were no significant differences in transport rates, retention rates and uptake rates of WHP-Zn, SHP-Zn and SP-Zn. NCP-Zn could improve the activity of Zn-related enzymes, and the expression levels of PepT1 and ZnT1 were higher than other peptide-Zn complexes. CONCLUSION: The promotion zinc absorption capacity of peptide-Zn complexes was not completely dependent on the Zn-binding capacity, but also depended on the charge and hydrophobicity of peptides. © 2024 Society of Chemical Industry.

[Ecological sensitivity of jingnanxia-heishanxia reach of Yellow River].

Aiming at the ecological problems such as soil erosion, desertification, and salinization in the Jingnanxia-Heishanxia reach of Yellow River, the single-factorial ecological problem's sensitivity and the comprehensive ecological sensitivity of the reach were analyzed by GIS spatial analysis, grid computing and superposition, and RS image feature extraction. The results showed that the regions with medium- and high ecological sensitivities almost covered the whole study area, including the Wufo Town of Jingtai County, the Beiwan Town, Wulan Town, Mitan Town, Dongwan Town, and Santan Town of Jingyuan County, and the area between Doucheng and Hongliutan of Pingchuan District. The high, medium, and low sensitive areas should be accordingly programmed as forbidden, medium, and deep constructing areas. During the development process of regional hydropower, effective measures should be adopted to protect the ecological environment to achieve the sustainable development of the drainage area.