Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 5 de 5
Filtrar
Mais filtros

Base de dados
Tipo de documento
Intervalo de ano de publicação
1.
Plant J ; 2024 Aug 08.
Artigo em Inglês | MEDLINE | ID: mdl-39115043

RESUMO

The biogenesis of functional forms of chloroplast ribosomal RNAs (rRNAs) is crucial for the translation of chloroplast mRNAs into polypeptides. However, the molecular mechanisms underlying the proper processing and maturation of chloroplast rRNA species are poorly understood. Through a genetic approach, we isolated and characterized an Arabidopsis mutant, α1-4, harboring a missense mutation in the plastid chaperonin-60α1 gene. Using allelism tests and transgenic manipulation, we determined functional redundancy among ptCPN60 subunits. The ptCPN60α1S57F mutation caused specific defects in the formation of chloroplast rRNA species, including 23S, 5S, and 4.5S rRNAs, but not 16S rRNAs. Allelism tests suggested that the dysfunctional ptCPN60α1S57F competes with other members of the ptCPN60 family. Indeed, overexpression of the ptCPN60α1S57F protein in wild-type plants mimicked the phenotypes observed in the α1-4 mutant, while increasing the endogenous transcriptional levels of ptCPN60α2, ß1, ß2, and ß3 in the α1-4 mutant partially mitigated the abnormal fragmentation processing of chloroplast 23S, 5S, and 4.5S rRNAs. Furthermore, we demonstrated functional redundancy between ptCPN60ß1 and ptCPN60ß2 in chloroplast rRNA processing through double-mutant analysis. Collectively, our data reveal a novel physiological role of ptCPN60 subunits in generating the functional rRNA species of the large 50S ribosomal subunit in Arabidopsis chloroplasts.

2.
Chem Commun (Camb) ; 59(39): 5926-5929, 2023 May 11.
Artigo em Inglês | MEDLINE | ID: mdl-37098685

RESUMO

An in situ electrochemical reduction strategy is proposed to avoid the aggregation of nano-Ru in lithium batteries for the first time. The high-dispersion face-centered cubic (fcc) nano-Ru is successfully synthesized with an average diameter of 2.0 nm, and the as-assembled lithium-oxygen batteries deliver an excellent cycling performance of 185 cycles and an ultralow overpotential of only 0.20 V at 100 mA g-1.

3.
Plant J ; 114(1): 96-109, 2023 04.
Artigo em Inglês | MEDLINE | ID: mdl-36705084

RESUMO

Ribosome biogenesis is a process of making ribosomes that is tightly linked with plant growth and development. Here, through a suppressor screen for the smo2 mutant, we found that lack of a ribosomal stress response mediator, ANAC082 partially restored growth defects of the smo2 mutant, indicating SMO2 is required for the repression of nucleolar stress. Consistently, the smo2 knock-out mutant exhibited typical phenotypes characteristic of ribosome biogenesis mutants, such as pointed leaves, aberrant leaf venation, disrupted nucleolar structure, abnormal distribution of rRNA precursors, and enhanced tolerance to aminoglycoside antibiotics that target ribosomes. SMO2 interacted with ROOT INITIATION DEFECTIVE 2 (RID2), a methyltransferase-like protein required for pre-rRNA processing. SMO2 enhanced RID2 solubility in Escherichia coli and the loss of function of SMO2 in plant cells reduced RID2 abundance, which may result in abnormal accumulation of FIBRILLARIN 1 (FIB1) and NOP56, two key nucleolar proteins, in high-molecular-weight protein complex. Taken together, our results characterized a novel plant ribosome biogenesis factor, SMO2 that maintains the abundance of RID2, thereby sustaining ribosome biogenesis during plant organ growth.


Assuntos
Proteínas de Arabidopsis , Arabidopsis , Arabidopsis/metabolismo , Proteínas de Arabidopsis/genética , Proteínas de Arabidopsis/metabolismo , Nucléolo Celular/genética , Plantas/metabolismo , Ribossomos/metabolismo , RNA Ribossômico/genética , RNA Ribossômico/metabolismo
4.
Plant Physiol ; 180(2): 827-836, 2019 06.
Artigo em Inglês | MEDLINE | ID: mdl-30910906

RESUMO

The rapid appearance of herbicide-resistant weeds combined with a lack of novel herbicides being brought to market reduces crop production, thereby threatening food security worldwide. Here, we report on the use of the previously identified cellulose biosynthesis-inhibiting chemical compound C17 as a potential herbicide. Toxicity tests showed that C17 efficiently inhibits the growth of various weeds and widely cultivated dicotyledonous crops, whereas only slight or no growth inhibition was observed for monocotyledonous crops. Surprisingly, when exposed to a mixture of C17 and one of two well-known cellulose biosynthesis inhibitors (CBIs), isoxaben and indaziflam, an additive growth inhibition was observed, demonstrating that C17 has a different mode of action that can be used to sensitize plants toward known CBIs. Moreover, we demonstrate that a C17-resistant CESA3 allele can be used as a positive transformation selection marker and that C17 resistance can be obtained through genome engineering of the wild-type CESA3 allele using clustered regularly interspaced short palindromic repeats-mediated base editing. This editing system allowed us to engineer C17 tolerance in an isoxaben-resistant line, resulting in double herbicide-resistant plants.


Assuntos
Proteínas de Arabidopsis/genética , Arabidopsis/genética , Celulose/antagonistas & inibidores , Inibidores Enzimáticos/farmacologia , Edição de Genes , Glucosiltransferases/genética , Benzamidas/farmacologia , Membrana Celular/metabolismo , Celulose/metabolismo , Indenos/farmacologia , Plantas Daninhas/efeitos dos fármacos , Plantas Daninhas/crescimento & desenvolvimento , Mutação Puntual/genética , Relação Estrutura-Atividade , Triazinas/farmacologia
5.
Chem Commun (Camb) ; 53(76): 10568-10571, 2017 Sep 21.
Artigo em Inglês | MEDLINE | ID: mdl-28894872

RESUMO

We report a novel and facile route to improve the catalytic performance of carbon paper-based electrocatalysts, which can be activated by in situ discharging with the assistance of a Li cell. Our results show that the OER potentials were successfully reduced to ∼3.5 V, much lower than the value of 4.5 V for graphite paper, which is the best result recorded amongst the reported carbon-based electrocatalysts.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA