RESUMO
Apparently, the genomes of many organisms are pervasively transcribed, and long noncoding RNAs (lncRNAs) make up the majority of cellular transcripts. LncRNAs have been reported to play important roles in many biological processes; however, their effects on locomotion are poorly understood. Here, we presented a novel lncRNA, Locomotion Regulatory Gene (LRG), which participates in locomotion by sequestering Synaptotagmin 1 (SYT1). LRG deficiency resulted in higher locomotion speed which could be rescued by pan-neuronal overexpression but not by limited ellipsoid body, motoneuron or muscle-expression of LRG. At the molecular level, the synaptic vesicles (SVs) release and movement-related SYT1 protein was recognized as LRG-interacting protein candidate. Furthermore, LRG had no effects on SYT1 expression. Genetically, the behavioral defects in LRG mutant could be rescued by pan-neuronal knock-down of Syt1. Taken together, all the results suggested LRG exerts regulatory effects on locomotion via sequestering SYT1 thereby blocking its function without affecting its expression. Our work displays a new function of lncRNA and provides insights for revealing the pathogenesis of neurological diseases with motor disorders.
RESUMO
The protonation abilities of phthalocyaninatometals (MPcs) increase but their stabilities reduce by the introduction of alkoxyl substituents at alpha position. In the toluene, the order of mono-protonation rate for the tetra-alpha-(2, 2, 4-trimethyl-3-pentoxy)phthalocyaninatometals sorts with the center metals is Zn>Co>Cu>Ni>Fe, which is opposite to the order of their wavelength difference between the Q bands and X bands. However, their mono-protonated species can be decomposed easily at the rate order FePc>CoPc>CuPc>NiPc>ZnPc, analogous to their decomposition abilities in the benzoylperoxide (BPO) oxidation. In addition, it is interesting that a more remarkable decomposition is found when partial CuPc was mono-protonated.