Effects of Wx and Its Interaction With SSIII-2 on Rice Eating and Cooking Qualities.

The Wx gene encodes a granule-bound starch synthase (GBSS) and plays a key role in determining rice eating and cooking qualities (ECQs). SSIII-2 (SSIIIa), a member of the soluble starch synthases, is responsible for the synthesis of long chains of amylopectin. To investigate the effects of Wx and its interaction with SSIII-2 on grain ECQs, a population from a hybrid combination was established as a research material. The genotypes of SSIII-2 and the single nucleotide polymorphisms (SNPs) on intron1, exon6, and exon10 of Wx, and the physicochemical indicators and rapid visco analyzer (RVA) profile characteristics were analyzed. The results revealed various effects of SSIII-2 on rice quality under different backgrounds of Wx alleles. There was no obvious difference between different SSIII-2 alleles under the same background of Wxa , whereas there was a significant diversity under the same background of Wxb . Wxa had a dominant epistasis to SSIII-2 because the effect of SSIII-2 was masked by the massive synthesis of GBSS under Wxa . The apparent amylose content (AAC) was mainly controlled by the In1G/T SNP, and rice gel consistency (GC) was regulated by the Ex10C/T SNP. The combined effects of three SNPs had a significant influence on all ECQs and RVA profile parameters, except for gelatinization temperature. In1T-Ex6A-Ex10C and In1T-Ex6A-Ex10T were classified as being low AAC type. TT-AA-CC and TT-AA-TT had a low AAC and a soft GC. The combined effects of different SNPs of Wx are very important for rice quality breeding.

Combined effects of Wx and SSIIa haplotypes on rice starch physicochemical properties.

BACKGROUND: Wx and SSIIa are central genes for determining starch physicochemical properties and rice endosperm starch is composed of linear amylose, which is entirely synthesized by granule bound starch synthase I (GBSSI, encoded by Wx) and branched amylopectin. In the present study, different haplotypes of rice were examined to investigate the combined effects of pivotal genes in the metabolic chain of starch, Wx and SSIIa. RESULTS: Wx haplotypes differed in terms of apparent amylose content (AAC) and gel consistency (GC). The I-3 [haplotype I (Int1T/Ex10C) of Wx and haplotype 3 (A-G-TT) of SSIIa] and the I-4 combinations of rice had better eating and cooking qualities (ECQs) with lower AAC, lower gelatinization temperature (GT) and softer GC. CONCLUSION: The characteristic parameters of Rapid Visco-analyser (RVA) could distinguish differences in AAC and GC but not GT. The I-3 and I-4 haplotype combinations of Wx and SSIIa represent key targets for the production of rice with better ECQs. © 2016 Society of Chemical Industry.