Large-scale pattern transfer based on non-through-hole AAO self-supporting membranes.

Fabricating large-scale nanoarrays is a significant and challenging work in the field of nanometer devices. Anodic aluminum oxide (AAO) membrane is considered as a promising mask due to its inherent advantages such as low-cost and tunable pore diameter. However, there are few reports on the use of non-through-hole large-area AAO membrane as a mask. Due to its higher mechanical strength, non-through-hole AAO membrane has the advantage of self-supporting for large-area fabrication. Herein, we present a robust approach to transferring nanopattern to substrates with high fidelity by using the non-through-hole AAO membrane as an etching mask. A novel two-step inductively coupled plasma (ICP) etching method is adopted. The morphological evolution of the AAO during ICP etching is systematically investigated. The aspect ratio of the AAO can be quantitatively controlled by adjusting etching time. The AAO nanopore arrays with an area of 7.1 cm2 are successfully transferred to gallium nitride wafer to enhance photoluminescence. The luminous intensity of the nano-array LED with a pore diameter of 400 nm and a depth of 150 nm is improved by 3.4 times compared with the LED without the nano-array. This method extends the opportunities for AAO mask to serve as generic templates for novel applications that are previously impractical due to the difficulty of large-scale nano-pattern transfer.

Visual in vivo degradation of injectable hydrogel by real-time and non-invasive tracking using carbon nanodots as fluorescent indicator.

Visual in vivo degradation of hydrogel by fluorescence-related tracking and monitoring is crucial for quantitatively depicting the degradation profile of hydrogel in a real-time and non-invasive manner. However, the commonly used fluorescent imaging usually encounters limitations, such as intrinsic photobleaching of organic fluorophores and uncertain perturbation of degradation induced by the change in molecular structure of hydrogel. To address these problems, we employed photoluminescent carbon nanodots (CNDs) with low photobleaching, red emission and good biocompatibility as fluorescent indicator for real-time and non-invasive visual in vitro/in vivo degradation of injectable hydrogels that are mixed with CNDs. The in vitro/in vivo toxicity results suggested that CNDs were nontoxic. The embedded CNDs in hydrogels did not diffuse outside in the absence of hydrogel degradation. We had acquired similar degradation kinetics (PBS-Enzyme) between gravimetric and visual determination, and established mathematical equation to quantitatively depict in vitro degradation profile of hydrogels for the predication of in vivo hydrogel degradation. Based on the in vitro data, we developed a visual platform that could quantitatively depict in vivo degradation behavior of new injectable biomaterials by real-time and non-invasive fluorescence tracking. This fluorescence-related visual imaging methodology could be applied to subcutaneous degradation of injectable hydrogel with down to 7 mm depth in small animal trials so far. This fluorescence-related visual imaging methodology holds great potentials for rational design and convenient in vivo screening of biocompatible and biodegradable injectable hydrogels in tissue engineering.

Seed-mediated growth of ultra-thin triangular magnetite nanoplates.

We report a simple strategy for the growth of ultra-thin magnetite nanoplates. The injection of a large portion of precursor after stunted nucleation is favorable for both the survival of metastable structures in seeds and their subsequent development into anisotropic nanoparticles. The as-synthesized ultrathin triangular magnetite nanoplates are expected to have important applications as T1 contrast agents for magnetic resonance imaging.

UV-crosslinkable and thermo-responsive chitosan hybrid hydrogel for NIR-triggered localized on-demand drug delivery.

Innovative drug delivery technologies based on smart hydrogels for localized on-demand drug delivery had aroused great interest. To acquire smart UV-crosslinkable chitosan hydrogel for NIR-triggered localized on-demanded drug release, a novel UV-crosslinkable and thermo-responsive chitosan was first designed and synthesized by grafting with poly N-isopropylacrylamide, acetylation of methacryloyl groups and embedding with photothermal carbon. The UV-crosslinkable unit (methacryloyl groups) endowed chitosan with gelation via UV irradiation. The thermo-responsive unit (poly N-isopropylacrylamide) endowed chitosan hydrogel with temperature-triggered volume shrinkage and reversible swelling/de-swelling behavior. The chitosan hybrid hydrogel embedded with photothermal carbon exhibited distinct NIR-triggered volume shrinkage (∼42% shrinkage) in response to temperature elevation as induced by NIR laser irradiation. As a demonstration, doxorubicin release rate was accelerated and approximately 40 times higher than that from non-irradiated hydrogels. The UV-crosslinkable and thermal-responsive hybrid hydrogel served as in situ forming hydrogel-based drug depot is developed for NIR-triggered localized on-demand release.

Ultrahigh-yield synthesis of N-doped carbon nanodots that down-regulate ROS in zebrafish.

Oxidative damage induced by accumulation of excessive reactive oxygen species (ROS) could result in increased chronic inflammation and thus ageing and age-related diseases. Carbonaceous nanodrugs hold great promise for ameliorating age-related diseases, and it is necessary to develop ultrahigh-yield synthesis of such nanodrugs. To improve the synthetic yield (less than 50%) of carbon nanodots (CNDs), the general choice is to screen precursors. However, no reliable concept for improving the yield has been explored over the past few decades. We are the first to propose the concept of using carbon-carbon double bonds to boost the synthetic yield and demonstrate record breaking ultrahigh-yield (85.9%) synthesis of N-doped CNDs. When the C[double bond, length as m-dash]C content increased from 14 to 56 mmol, the synthetic yield exhibited a 3.3-fold increase. Nitrogen elements are doped as pyridinic-like N and NH2, where conjugated π-systems as electron donors and pyridinic-like structures would benefit the potential down-regulated effect for ROS. N-doped CNDs exhibit an outstanding protective effect against oxidative stress via inhibiting exogenous and endogenous ROS generation, where the ROS in zebrafish are significantly reduced by 68%. Hence the concept of carbon-carbon double bond-boosted ultrahigh-yield synthesis of N-doped CNDs provides a promising strategy to be employed for carbonaceous nanodrugs aiming at preventing and curing ageing and age-related diseases.