RESUMO
Serum pharmacochemistry of traditional Chinese medicine(TCM) is an effective method to rapidly screen the effective substances and reveal the compatibility law of compound by identification and analysis of constituents migrating to blood after oral administration. In the last two decades, it has been universally accepted and widely applied in the field. With the cross-fusion with other disciplines, such as serum pharmacology, pharmacokinetics, metabolomics, network pharmacology and systems biology, serum pharmacochemistry shows comprehensive superiority in explaining drug changes in vivo and in vitro, interactions between drugs, interactions between drug and body, which coincides with the complexity of TCM compatibility, multi-components, multi-targets and multi-mechanisms. Based on the references related with the serum pharmacochemistry from CNKI scholar and Pubmed in 2013-2016, the research results of serum pharmacochemistry were statistically analyzed, and the key technical problems during the study of serum pharmacochemistry, for example, preparation of test sample, selection of experimental animal, determination of drug delivery scheme, method and time of the adoption blood, preparation and pretreatment of blood sample, as well as analysis of constituents migrating to blood, and the solving ways were empirically introduced. In addition, the development and comprehensive application of serum pharmacochemistry in TCM were summarized in this paper, hoping to lay a foundation for the further application of this method in TCM research.
Assuntos
Avaliação Pré-Clínica de Medicamentos , Medicina Tradicional Chinesa , Soro/química , Animais , Medicamentos de Ervas Chinesas , Metabolômica , Biologia de SistemasRESUMO
Twenty-two compounds were isolated from the flowers of Scabiosa tschilliensis. Their structures were identified by spectroscopic methods as octacosanol (1), stearic acid (2), ß-sitosterol (3), oleanolic acid (4), apigenin (5), luteolin (6), daucosterol (7), kaempferol-3-O-ß-D-6-O-(p-hydroxycinnamoyl) -glucopyranoside (8), kaempferol-3-O-ß-D- (3, 6-di-p-(hydroxycinnamoyl) -glucopyranoside (9), apigenin-7-O-ß-D-glucopyranoside (10), luteolin-4'-O-ß-D-glucopyranoside (11), apigenin-7-O-rutinoside (12), luteolin-7-O-ß-D-glucopyranoside (13), apigenin-4'-O-ß-D-glucopyranoside (14), caffeic acid methyl ester (15), loganin (16), adenosine (17), luteolin-6-C-ß-D-glycopyranosyl (18), sweroside (19), sylvestrosides I (20), sylvestrosides II (21), urceolide (22). Among them, compounds 1, 2, 7-9, 12, 15, 17-18, 20-22 were isolated from the genus Scabiosa for the first time, and compounds 1-4, 6-9, 11-12, 14-22 were isolated from this plant for the first time. 13C-NMR data of 22 were reported for the first time.
Assuntos
Dipsacaceae/química , Medicamentos de Ervas Chinesas/química , Flores/química , Estrutura Molecular , Espectrometria de Massas por Ionização por ElectrosprayRESUMO
Two new sesquiterpenes, innatifolone A (1) and pinnatifolone B (2), along with 6 known compounds, furostan (3), isocalamendiol (4), pluviatolide (5), (8S,8'R,9S)-cubebin (6), 2-(4-hydroxy-3-methoxybenzyl)-3-(3,4-dimethoxybenzyl) tetrahydrofuran (7), and methyl 3-acetoxy-12-oleanen-28-oate (8), were isolated from Mongolian herb Syringa pinnatifolia.
Assuntos
Medicamentos de Ervas Chinesas/química , Plantas Medicinais/química , Syringa/química , Medicamentos de Ervas Chinesas/isolamento & purificação , Espectrometria de Massas , Estrutura Molecular , MongóliaRESUMO
Four new furostanol glycosides were isolated from the flowers of Hosta plantaginea (Lam.) Aschers. On the basis of spectroscopic methods including 1D and 2D NMR experiments, their structures were elucidated as 26-O-ß-d-glucopyranosyl-(25R)-22-O-methyl-5α-furostan-2α,3ß,22ξ,26-tetrol 3-O-α-l-rhamnopyranosyl-(1 â 4)-O-ß-d-xylopyranosyl-(1 â 3)-[O-ß-d-glucopyranosyl-(1 â 2)]-O-ß-d-glucopyranosyl-(1 â 4)-ß-d-galactopyranoside (hostaplantagineoside A, 1), 26-O-ß-d-glucopyranosyl-(25R)-5α-furostan-20(22)-ene-2α,3ß,26-triol-3-O-ß-d-glucopyranosyl-(1 â 2)-[O-ß-d-xylopyranosyl-(1 â 3)]-O-ß-d-glucopyranosyl-(1 â 4)-ß-d-galactopyranoside (hostaplantagineoside B, 2), 26-O-ß-d-glucopyranosyl-(25R)-5α-furostan-22(23)-ene-2α,3ß,20α,26-tetraol-3-O-ß-d-glucopyranosyl-(1 â 2)-[O-ß-d-xylopyranosyl-(1 â 3)]-O-ß-d-glucopyranosyl-(1 â 4)-O-ß-d-galactopyranoside (hostaplantagineoside C, 3), 26-O-ß-d-glucopyranosyl-(25R)-5α-furostan-20(22)-ene-2α,3ß,26-triol-3-O-α-l-rhamnopyranosyl-(1 â 4)-O-ß-d-xylopyranosyl-(1 â 3)-[O-ß-d-glucopyranosyl-(1 â 2)]-O-ß-d-glucopyranosyl-(1 â 4)-ß-d-galactopyranoside (hostaplantagineoside D, 4).
Assuntos
Medicamentos de Ervas Chinesas/química , Medicamentos de Ervas Chinesas/isolamento & purificação , Glicosídeos/química , Glicosídeos/isolamento & purificação , Hosta/química , Esteróis/química , Esteróis/isolamento & purificação , Estrutura Molecular , Ressonância Magnética Nuclear Biomolecular , Fitosteróis/química , EstereoisomerismoRESUMO
OBJECTIVE: To investigate the chemical constituents of Potentilla multifida. METHOD: Various chromatographic techniques were employed for isolation and purification of the constituents. The structures were elucidated by spectral analysis. RESULT: Four megastigmane glycosides were isolated from P. multifida and their structures were identified as citroside A (1), icariside B1 (2), (6S,7E,9R)-roseoside (3), (6S,7E,9R)-vomifoliol-9-O-beta-D-xylopyranosyl-(1-->6)-O-beta-D-glucopyranoside (4), respectively. CONCLUSION: All compounds were obtained from the genus Potentilla for the first time.
Assuntos
Glicosídeos/isolamento & purificação , Norisoprenoides/isolamento & purificação , Plantas Medicinais/química , Potentilla/química , Glicosídeos/química , Estrutura Molecular , Norisoprenoides/químicaRESUMO
OBJECTIVE: To study the chemical constituents of Potentilla multifida L. METHODS: Chemical constituents were isolated by the repeated silica gel chromatography and Sephadex LH-20, and their structures were identified by the spectral analysis. RESULTS: Five compounds were obtained as follows: 3beta,24-dihydroxyl-urs-12-ene (1), ursolic acid (2), euscaphic acid (3), tormentic acid (4), and epihedaragenin (5). CONCLUSION: Five compounds were isolated from this plant for the first time. Compounds 1 and 5 were isolated from this genus for the first time. Compound 1 was a new natural product.