RESUMO
OBJECTIVE: To study the chemical constituents from the leaves of Acanthus ilicifolius. METHODS: The compounds were isolated by silica and gel column chromatographic methods and identified by spectoscopic analysis. The anti-influenza virus activities of these compounds were obtained by measuring the neuraminidase activity of influenza virus. RESULTS: Five compounds were isolated and their structures were identified as blepharin(1), acteoside(2), isoverbascoside(3), daucosterol(4), and 3-O-ß-D-glucopyranosyl-stigmasterol(5). CONCLUSION: All the compounds are isolated from the leaves of Acanthus ilicifolius for the first time, and compounds 1 ~ 3 exhibit the anti-influenza virus activities.
Assuntos
Acanthaceae/química , Antivirais/química , Orthomyxoviridae/efeitos dos fármacos , Compostos Fitoquímicos/química , Folhas de Planta/química , Antivirais/isolamento & purificação , Glucosídeos , Fenóis , Compostos Fitoquímicos/isolamento & purificação , Sitosteroides , EstigmasterolRESUMO
Alzheimer's disease (AD) is a neurodegenerative disorder that affects the elderly population. Deposition of beta-amyloid (Aß) in the brain is a hallmark of AD pathology. In our previous study, we have constructed a cell line expressing human APP695 (hAPP695) in SH-EP1 cells stably transfected with human nicotinic receptor (nAChR) α4 subunit and ß2 subunit gene. In present study, we found that activation of α4ß2 nAChR by nicotine and epibatidine decreased secreted Aß level in the cell line and hippocampal neurons, but had no effects on full-length APP695 and sAPP-α. Nicotine also decreases BACE1 and PSEN1 expression, as well as ERK1 and NFκB P65 subunit expression in the cell line. Furthermore, BACE1 promoter activity is, but PSEN1 not, decreased by nicotine in the cell line. All the results suggest that activation of α4ß2 nAChR decreases Aß through regulating BACE1 transcription by ERK1-NFκB pathway. Additionally, analysis of BACE1 promoter activity by dual-luciferase reporter assay may be useful for drug screening as a high throughput method.
Assuntos
Secretases da Proteína Precursora do Amiloide/genética , Peptídeos beta-Amiloides/metabolismo , Ácido Aspártico Endopeptidases/genética , Nicotina/farmacologia , Transcrição Gênica/efeitos dos fármacos , Precursor de Proteína beta-Amiloide/genética , Linhagem Celular , Ensaio de Imunoadsorção Enzimática , Humanos , Reação em Cadeia da Polimerase , Receptores Nicotínicos/genéticaRESUMO
OBJECTIVE: To establish an HPLC method for determination of hedychenone in Hedychium yunnanense. METHOD: C18 Chromatographic column was used, acetonitrile-water (9:1) as mobile phase, at flow rate of 1.0 mL x min(-1): The wavelength for detection was 235 nm. RESULT: The linear range of hedychenone was 5.92-29.6 microg x mL(-1)(r = 0.9999). The average recovery was 99.0%, RSD of precision was less than 2%. CONCLUSION: The method is simple, effective and feasible, and can be used to evaluate the quality of the herb.