RESUMO
The aim of this study was to evaluate the effect of Hermetia illucens larvae meal (HI) on the growth performance and intestinal barrier function of weaned pigs. To achieve this, 72 weaned pigs [28-day-old, 8.44 ± 0.04 kg body weight (BW)] were randomly assigned to three dietary treatments: basal diet (negative control, NC), zinc oxide-supplemented diet (positive control, PC), and HI-supplemented diet [100% replacement of fishmeal (FM), HI], for 28 days in the presence of enterotoxigenic Escherichia coli (ETEC). The results showed that HI and PC increased (p < 0.05) the average daily gain (ADG) and average daily feed intake (ADFI) of weaned pigs from day 1 to 14, and decreased diarrhea incidence from day 1 to 28. Additionally, HI increased (p < 0.05) claudin-1, occludin, mucin-1 (MUC-1), and MUC-2 expression, goblet cell number, and secretory immunoglobulin A (sIgA) concentration in the intestine of weaned pigs. Compared with NC, HI downregulated (p < 0.05) interleukin-1ß (IL-1ß) and IL-8 expression, and upregulated IL-10, transforming growth factor-ß (TGF-ß), antimicrobial peptide [porcine ß defensin 1 (pBD1), pBD2, protegrin 1-5 (PG1-5)] expression in the jejunum or ileum. Moreover, HI decreased (p < 0.05) toll-like receptor 2 (TLR2), phosphorylated nuclear factor-κB (p-NF-κB), and phosphorylated mitogen-activated protein kinase (p-MAPK) expression, and increased sirtuin 1 (SIRT1) expression in the ileum. Additionally, HI increased histone deacetylase 3 (HDAC3) expression and acetylation of histone 3 lysine 27 (acH3k27) in the ileum. Furthermore, HI positively influenced the intestinal microbiota composition and diversity of weaned pigs and increased (p < 0.05) butyrate and valerate concentrations. Overall, dietary HI improved growth performance and intestinal barrier function, as well as regulated histone acetylation and TLR2-NF-κB/MAPK signaling pathways in weaned pigs.
RESUMO
DL-2-hydroxy-4(methylthio) butanoic acid (DL-HMTBA) exhibits a higher antioxidant capability in vitro as compared to DL-Met, but the mechanism is still not known. A total of 400 8-day-old broiler chicks were allotted to a 2 [low (12 to 14°C) vs. control temperature (thermoneutral, 24 to 26°C)] × 2 (0.17% or 0.51% of DL-HMTBA) factorial arrangement to investigate effects of DL-HMTBA on antioxidant capacity and its related gene expression in lung and liver of broilers exposed to low temperature. The hepatic glutathione (GSH), superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) activities were decreased, whereas protein carbonyl and malodndialdehyde contents in lung were increased in low temperature (P < 0.05). Dietary supplementation of 0.51% DL-HMTBA increased (P < 0.05) GSH and GSH-Px activity in liver and SOD activity in lung in spite of the temperature. The greater gene expression of GSH reductase and lower expression of GSH synthetase (P < 0.01) were observed in lung of broilers that were maintained at low temperature. Higher DL-HMTBA supplementation induced greater (P < 0.05) mRNA expression of glutathione-S transferase in lung, GSH synthetase in liver and lung, as well as lower expression of GSH reductase in lung, and this effect were more obvious for the chicks exposed to low temperature (interaction, P < 0.05). Chicks that were maintained under low temperature had the lower expression of cystathionine ß-synthase (CßS), whereas those fed 0.51% DL-HMTBA increased the CßS expression in liver under low temperature (interaction, P ≤ 0.05). The γ-glutamylcysteine synthetase and Met adenosyltransferase 1 (MAT1) gene expression were downregulated (P < 0.05) by low temperature and the higher (P = 0.02) expression of MAT1 was observed in lung of chicks fed 0.51% DL-HMTBA. It indicated that under low temperature, DL-HMTBA supplementation at 0.51% upregulated gene expression of GSH synthesis and Met transsulfuration pathway in liver and lung to increase the antioxidant capacity, and then mitigate the negative effects of cold stress for broilers.