Developing a 3D animation tool to improve veterinary undergraduate understanding of obstetrical problems in horses.

BACKGROUND: Many challenges are encountered in both teaching and learning veterinary obstetrics. This may be due to outdated teaching materials, as the main model of content transmission remains centred around text and images. METHODS: Visualisation methods such as three-dimensional (3D) and Graphics Interchange Format (GIF) tools were applied in an attempt to improve obstetrics education outcomes in the third-year class. Traditional teaching methods were utilised in the fourth-year and fifth-year students. RESULTS: These supplementary tools significantly increased the third-year students' final examination results compared with the results of fourth-year and fifth-year students (P<0.05). These examinations were designed to evaluate comprehension of the subject matter. Self-assessment questionnaire results further indicated that 3D animation and GIF promoted learning efficiency. CONCLUSION: Incorporation of 3D animation learning tools into the veterinary curriculum is predicted to better prepare students for the management of obstetrical cases after graduation.

[Immediate-early c-fos and tyrosine hydroxylase protein expression in different brain regions in high-fat induced obesity in mice].

OBJECTIVE: To examine brain dopamine expression in chronic high-fat diet(HFD)-induced obese mice. METHODS: Ten male mice were fed by a high-fat diet (HF:45% of calories from fat) for 12 weeks and then classified as HFD group. Ten male mice were fed a low-fat diet (LF:10% of calories from fat) and used as control group (NCD). In the 10th week, the blood of the caudal vein was collected to determine the basal blood glucose level after both groups mice were fast for 12 h. Intraperitoneal (IP) glucose tolerance test (GTT) and insulin tolerance (ITT) were performed in HFD and NCD mice in the 12th week. Animals were sacrificed after fasting for 4 hours at the 12th week. Brain tissues were processed for Fos-ir and TH-ir by immunohistochemistry. RESULTS: After 12 weeks of feeding, body weight was significant higher in HFD mice than that in NCD ones. During GTT and ITT, HFD mice had significantly decreased glucose tolerance and insulin tolerance at 15 min and 30 min respectively than NCD ones (P<0.05). There were higher plasma insulin concentration and leptin concentration in HFD mice than those in NCD ones (P<0.05). High fat-induced increased body weight was associated with increased cellular activation, indicated by Fos immunoreactive (ir) staining, in nucleus accumbens(NAcc), paraventricular nucleus (PVN), ventral tegmental area (VTA) and substantia nigra (SN) than those of NCD ones (P<0.05); and also significantly associated with enhanced in the number of cells labeled for tyrosine hydroxylase (TH-ir), and the number of cells co-labeled for TH-ir/Fos-ir in the VTA and SN than those of NCD ones (P<0.01). Moreover, there was significantly relationship TH-ir positive cell numbers with final body weight in VTA and SN in HFD mice (P<0.05). CONCLUSIONS: The results showed that chronic consumption of high-fat food was associated with plasticity-related changes in reward circuitry in mice.

[Effect of dopamine receptor agonist apomorphine on scopolamine induced memory deficits in mice].

OBJECTIVE: To research the mechanism of dopamine (DA) controlled memory in mice. METHODS: Mice received i.p. injection of scopolamine (0.3 mg/kg, SCOP 0.3, and 3.0mg/kg, SCOP 3.0, respectively, n = 10) and saline (NS, n = 10) for 60 days in experiment 1. Memory of mice was detected by dark avoidance behavior in the 53" d and the 60"' d. Animals were sacrificed after the memory test; brain tissues were processed for Fos-ir and TH-ir by immunohistochemistry. Mice were divided into four groups according results of expri-ment 1, they received i.p. injection of apomorphine (0.1 mg/kg, APO 0.1, 0.5 mg/kg, APO 0.5, and 2.0 mg/kg, APO2.0 respectively, n = 10). RESULTS: Memory was inhibited in mice injected scopolamine 3.0 mg/kg. Latency was significantly less than in NS group, only 1/ 4 that of NS group (P > 0.05). The number of mistake of SCOP 3.0 group increased about four times than that of NS group (P > 0.05). But there was no difference of latency and number of mistake between SCOP 0.3 and NS group in expriment 1. Scopolamine-induced memory deficit was associated with decreased cellular activation, indicated by Fos immunoreactive (ir) staining, in NAcc CA1 and CA3 (P < 0.05), and also associated with decreases in the number of cells labeled for tyrosine hydroxylase (TH-ir), the rate limiting enzyme for dopamine conversion (P < 0.01) and the number of cells co-labeled for TH-ir/Fos-ir (P <0.01) in the ventral tegmental area(VTA), apomorphine lessened scopolamine-induced memory deficit in experiment 2. The number of cells co-labeled for TH-ir/Fos-ir (P <, 0.05) was increased in VTA after apomorphine treatment. CONCLUSION: Apomorphine lessened scopolamine-induced memory deficit in mice by increasing DA activities in VTA.

Food hoarding, but not food intake, is attenuated by acute diazepam treatment in female Mongolian gerbils (Meriones unguiculatus).

This article is part of a Special Issue "Energy Balance". Effects of γ-aminobutyric acid (GABA) on food hoarding are unknown in rodents, and the effects of energy balance and GABA have not been evaluated in females. To evaluate the role of food deprivation and GABA on food hoarding, female Mongolian gerbils were given i.p. injection of diazepam (1mg/kg and 3mg/kg, respectively), a GABAA receptor agonist. Among food-deprived females, there was a bimodal pattern in the frequency of gerbils with different levels of food hoarding. High food hoarding (HFH) and low food hoarding (LFH) gerbils were analyzed. Diazepam blocked food deprivation-induced food hoarding in HFH gerbils, but not in LFH gerbils. This blockade was associated with increased cellular activation in selected brain areas, such as the nucleus accumbens (NAcc), caudate putamen (CP) and ventral tegmental area (VTA), which suggested that direct activation of GABA in the brain reward circuitry decreased food hoarding in HFH females. Moreover, diazepam increased Fos expression in field CA2 and CA3 of the hippocampus, but had no significant effect on Fos expression in field CA1 and dentate gyrus (DG) of the hippocampus, indicating that the hippocampus has area-specific effects on food hoarding in HFH gerbils. Diazepam did not alter food intake in both HFH and LFH gerbils. In addition, serum corticosterone concentrations were higher in the HFH than in the LFH ones. Together, these data indicated that food deprivation increased food hoarding in female gerbils, diazepam reduced food deprivation-induced food hoarding in HFH gerbils, and that GABA might influence food hoarding via classical reward circuitry via the mesolimbic dopamine system and specific hippocampal areas.

Increased feeding and food hoarding following food deprivation are associated with activation of dopamine and orexin neurons in male Brandt's voles.

Small mammals usually face energetic challenges, such as food shortage, in the field. They have thus evolved species-specific adaptive strategies for survival and reproductive success. In the present study, we examined male Brandt's voles (Lasiopodomys brandtii) for their physiological, behavioral, and neuronal responses to food deprivation (FD) and subsequent re-feeding. Although 48 hr FD induced a decrease in body weight and the resting metabolic rate (RMR), such decreases did not reach statistical significance when compared to the control males that did not experience FD. During the first 2 hr of re-feeding following 48 hr FD, voles showed higher levels of feeding than controls. However, when permitted to hoard food, FD voles showed an increase in food hoarding, rather than feeding, compared to the controls. Further, both feeding and food hoarding induced an increase in neuronal activation, measured by Fos-ir, in a large number of brain areas examined. Interestingly, feeding and food hoarding also induced an increase in the percentage of tyrosine hydroxylase immunoreactive (TH-ir) cells that co-expressed Fos-ir in the ventral tegmental area (VTA), whereas both FD and feeding induced an increase in the percentage of orexin-ir cells that co-expressed Fos-ir in the lateral hypothalamus (LH). Food hoarding also increased orexin-ir/Fos-ir labeling in the LH. Together, our data indicate that food-deprived male Brandt's voles display enhanced feeding or food hoarding dependent upon an environmental setting. In addition, changes in central dopamine and orexin activities in selected brain areas are associated with feeding and hoarding behaviors following FD and subsequent re-feeding.

Food hoarding and associated neuronal activation in brain reward circuitry in Mongolian gerbils.

Mongolian gerbils (Meriones unguiculatus) display food hoarding and thus provide an opportunity to study the neuromechanisms underlying this behavior. In the present study, male gerbils exhibited a bimodal expression of food hoarding behavior-some displayed high levels of food hoarding whereas others virtually lacked this behavior under normal laboratory conditions with free access to food. Food hoarding was found to be associated with an increase in neuronal activation, indicated by Fos immunoreactive (ir) staining, in several brain areas including the nucleus accumbens, ventral tegmental area (VTA), and lateral hypothalamus. Food hoarding was also associated with increases in the number of cells labeled for tyrosine hydroxylase (TH-ir), the rate limiting enzyme for dopamine conversion, and the number of cells co-labeled for TH-ir/Fos-ir in the VTA, suggesting that dopamine in the brain reward circuitry may be involved in food hoarding. Further, we found that 22 h of food deprivation induced food hoarding in some, but not all, males that naturally did not display food hoarding. In these males, however, food hoarding did not increase TH-ir or TH-ir/Fos-ir expression in the VTA. Together, these data indicate that male Mongolian gerbils display diverse phenotypes of food hoarding behavior and that dopamine in the brain reward circuitry may be involved in the control of naturally occurring, but not food deprivation-induced, food hoarding.

Memory performance of hypercholesterolemic mice in response to treatment with soy isoflavones.

The aim of this study is to investigate the memory performance of hypercholesterolemic mice in response to soy isoflavones (SI) treatment and the mechanism involved. In this study, 64 mice were randomly divided into four groups: control, high lipid diet without SI, high lipid diet with a low SI level (50 mg/kg bw) and high lipid diet with a high SI level (100 mg/kg bw). The experimental period was 30 days. The results indicated that the mice given the different treatments showed the different percentages of good, medium and poor memory performance. chi(2) analysis revealed significant difference in memory performance (P<0.05) between the high lipid diet without SI group and the high lipid diet with a low SI level group or high lipid diet with a high SI level group. Moreover, SI treatment resulted in a decrease in blood cholesterol (TC) level (high lipid diet without SI group versus high lipid diet with a low SI level group or high lipid diet with a high SI level group, P<0.05) and triglyceride (TG) level (high lipid diet without SI group versus high lipid diet with a low SI level group or high lipid diet with a high SI level group, P<0.05). In addition, SI treatment resulted in a significant decrease in acetylcholinesterase (AChE) activity and significant increases in glutamic acid and aspartic acid contents in the frontal cerebral cortex and hippocampus. The results suggest that SI improve the memory performance of hypercholesterolemic mice, and the mechanism underlying the improvement might closely correlate with its roles in decreasing high blood lipid levels and modulating the metabolism of neurotransmitters such as acetylcholine and amino acids in brain areas of hypercholesterolemic mice.