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BACKGROUND: X-ray fluorescence (XRF) emerges as a promising technique for estimating heavy metal elements. However, XRF spectra typically contain a significant amount of environmental information and signal noise, and the relationship between spectral intensity and element concentration is difficult to quantify using a single model, thereby reducing the predictive performance for low concentration elements. RESULTS: This paper proposed a comprehensive framework for predicting elemental concentrations, encompassing preprocessing, variable selection, decision-making, to enable fast, non-destructive, and accurate estimation of element concentrations in soil. Firstly, an optimal denoising method based on fractional discrete wavelet transform (FDWT) was introduced to enhance signal quality. Furthermore, the frequency-based competitive adaptive reweighted sampling (FCARS) algorithm was employed for feature selection of XRF spectral variables, allowing extraction of the most informative features from the complex spectral data. Finally, a novel deep learning network, called ConvBiLSTM-Attention (CBLA-Net), was designed to achieve precise estimation of heavy metal elements concentration. Compared with other advanced algorithms, The CBLA-Net demonstrated the highest accuracy for V, Cr, Mn, Zn, Cd, and Pb, achieving the coefficient of determination (R2) of 0.9730, 0.9874, 0.9952, 0.9921, 0.9518, and 0.9741, respectively. The CBLA-Net not only effectively extract local features and capture global information, but also combines attention mechanism to focus on key information. SIGNIFICANCE: The proposed novel deep learning quantitative framework, including preprocessing, feature selection, and CBLA-Net decision-making, significantly enhances the accuracy of elemental content prediction. It provides a new approach for accurately assessing the concentration of heavy metal elements in soil.
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Food fraud is widespread in the aquatic food market, hence fast and non-destructive methods of identification of fish flesh are needed. In this study, multispectral imaging (MSI) was used to screen flesh slices from 20 edible fish species commonly found in the sea around Yantai, China, by combining identification based on the mitochondrial COI gene. We found that nCDA images transformed from MSI data showed significant differences in flesh splices of the 20 fish species. We then employed eight models to compare their prediction performances based on the hold-out method with 70% training and 30% test sets. Convolutional neural network (CNN), quadratic discriminant analysis (QDA), support vector machine (SVM), and linear discriminant analysis (LDA) models perform well on cross-validation and test data. CNN and QDA achieved more than 99% accuracy on the test set. By extracting the CNN features for optimization, a very high degree of separation was obtained for all species. Furthermore, based on the Gini index in RF, 11 bands were selected as key classification features for CNN, and an accuracy of 98% was achieved. Our study developed a successful pipeline for employing machine learning models (especially CNN) on MSI identification of fish flesh, and provided a convenient and non-destructive method to determine the marketing of fish flesh in the future.
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Hyperuricaemia (HUA) is a metabolic disorder characterised by high blood uric acid (UA) levels; moreover, HUA severity is closely related to the gut microbiota. HUA is also a risk factor for renal damage, diabetes, hypertension, and dyslipidaemia; however, current treatments are associated with detrimental side effects. Alternatively, Fangyukangsuan granules are a natural product with UA-reducing properties. To examine their efficacy in HUA, the binding of small molecules in Fangyukangsuan granules to xanthine oxidase (XOD), a key factor in UA metabolism, was investigated via molecular simulation, and the effects of oral Fangyukangsuan granule administration on serum biochemical indices and intestinal microorganisms in HUA-model rats were examined. Overall, 24 small molecules in Fangyukangsuan granules could bind to XOD. Serum UA, creatinine, blood urea nitrogen, and XOD levels were decreased in rats treated with Fangyukangsuan granules compared to those in untreated HUA-model rats. Moreover, Fangyukangsuan granules restored the intestinal microbial structure in HUA-model rats. Functional analysis of the gut microbiota revealed decreased amino acid biosynthesis and increased fermentation of pyruvate into short-chain fatty acids in Fangyukangsuan granule-treated rats. Together, these findings demonstrate that Fangyukangsuan granules have anti-hyperuricaemic and regulatory effects on the gut microbiota and may be a therapeutic candidate for HUA.
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Modelos Animais de Doenças , Medicamentos de Ervas Chinesas , Microbioma Gastrointestinal , Hiperuricemia , Ácido Úrico , Animais , Hiperuricemia/tratamento farmacológico , Hiperuricemia/metabolismo , Microbioma Gastrointestinal/efeitos dos fármacos , Ratos , Medicamentos de Ervas Chinesas/farmacologia , Medicamentos de Ervas Chinesas/uso terapêutico , Masculino , Ácido Úrico/sangue , Xantina Oxidase/metabolismo , Ratos Sprague-DawleyRESUMO
This study aim to assess the clinical efficacy and safety of generic cefoperazone/sulbactam compared to the branded cefoperazone/sulbactam (Sulperazon) in treating bacterial infections through a meta-analysis. Searches were conducted across PubMed, Embase, Cochrane Library, CNKI, WanFang, VIP databases, and Clinical Trials database, resulting in the inclusion of 11 studies comprising 7 randomized controlled trials (RCTs) and 4 retrospective cohort studies (RCSs). Meta-analysis of the RCTs indicated no statistical differences in clinical success rates, clinical cure rates, microbiological eradication rates, and incidence of adverse reactions between the generic cefoperazone/sulbactam and the branded version. Findings from the RCSs aligned with those from the RCTs, demonstrating that generic versions of cefoperazone/sulbactam are equivalent in efficacy and safety to their branded counterparts in treating bacterial infections.
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A new disaccharide glycoside, franchoside A (1), and 17 known compounds were isolated from the tubers of Arisaema franchetianum Engler. The chemical structure of the previously undescribed compound 1 was elucidated on the basis of detailed spectroscopic analyses. Compounds 1, 2, 6, 10, 14 and 18 showed significant cytotoxic activities at varying IC50 values in the range of 4.0-10.6 µM against five cancer cell lines. Compounds 8, 10, 13 and 17 (10 µM) exhibited moderate anti-inflammatory activities by inhibiting the NF-κB signaling pathway and the release of NO from RAW264.7 macrophages induced by lipopolysaccharide (LPS), while compounds 1, 9, 14, 15 and 16 showed weak anti-inflammatory activities.
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Antineoplásicos , Arisaema , Glicosídeos/farmacologia , Glicosídeos/química , Linhagem Celular , Anti-Inflamatórios/farmacologia , Lipopolissacarídeos/farmacologiaRESUMO
RAD54B belongs to the SNF2/SWI2 superfamily, participating in homologous recombination repair. DNA damage is the central driver of aging, but there is no direct evidence of an association between RAD54B and vascular aging. The present study sought to investigate the role and mechanisms of RAD54B in endothelial senescence. In senescent animal models, including spontaneously hypertensive rats, normal aging mice, and D-gal-induced senescent mice, and senescent cell models induced by H2O2, D-gal, and culture, RAD54B was remarkably downregulated. Knockdown of RAD54B increased the expression of p53 and p21, increased the ratio of SA-ß-gal-positive cells, and decreased the proportion of EdU-positive cells. Conversely, overexpression of RAD54B reversed the senescent phenotypes stimulated by H2O2 and delayed replicative endothelial senescence. Mechanistically, silencing RAD54B compensatorily increased the expression of RAD51/XRCC4, which remained unchanged in H2O2-induced senescence. RAD54B lacking the SNF2 domain could still reverse the increasing expression of p53/p21 induced by H2O2. RAD54B reduced γH2A.X expression and inhibited the expression and phosphorylation of CHK1. In conclusion, RAD54B exerts a direct protective effect against DNA damage through enhancing homologous recombination repair in endothelial senescence, resulting in inhibition of the downstream CHK1/p53/p21 pathway, suggesting that RAD54B may be a potential therapeutic target for vascular aging-associated diseases.
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Senescência Celular , Proteína Supressora de Tumor p53 , Camundongos , Animais , Proteína Supressora de Tumor p53/metabolismo , Peróxido de Hidrogênio/toxicidade , Peróxido de Hidrogênio/metabolismo , Envelhecimento/metabolismo , Endotélio Vascular/metabolismoRESUMO
In this article, we investigate a novel but insufficiently studied issue, unpaired multi-view clustering (UMC), where no paired observed samples exist in multi-view data, and the goal is to leverage the unpaired observed samples in all views for effective joint clustering. Existing methods in incomplete multi-view clustering usually utilize the sample pairing relationship between views to connect the views for joint clustering, but unfortunately, it is invalid for the UMC case. Therefore, we strive to mine a consistent cluster structure between views and propose an effective method, namely selective contrastive learning for UMC (scl-UMC), which needs to solve the following two challenging issues: 1) uncertain clustering structure under no supervision information and 2) uncertain pairing relationship between the clusters of views. Specifically, for the first one, we design an inner-view (IV) selective contrastive learning module to enhance the clustering structures and alleviate the uncertainty, which selects confident samples near the cluster centroids to perform contrastive learning in each view. For the second one, we design a cross-view (CV) selective contrastive learning module to first iteratively match the clusters between views and then tighten the matched clusters. Also, we utilize mutual information to further enhance the correlation of the matched clusters between views. Extensive experiments show the efficiency of our methods for UMC, compared with the state-of-the-art methods.
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Two doses of the inactivated influenza vaccine (IIV) are generally recommended for children under 9 years old. This study assessed the necessity for a second dose of quadrivalent IIV (IIV4) in children aged 3-8 years. In this randomized, open-label, paralleled-controlled study, 400 children aged 3-8 years who were vaccine-unprimed were randomly assigned at a 1:1 ratio to receive a two-dose (Group 1) or one-dose (Group 2) regimen of IIV4, and 200 who were vaccine-primed received one dose of IIV4 (Group 3). A serum sample was collected before and 28 days after the last dose to determine the hemagglutination inhibition (HI) antibody level. Adverse events were collected within 28 days after each dose. One-dose or two-doses of IIV4 were well tolerated and safe in children aged 3-8 years, and no serious adverse events related to the vaccine were reported. The seroconversion rates (SCRs) of HI antibody ranged from 61.86% to 95.86%, and the post-vaccination seroprotection rates (SPRs) were all >70% in three groups against the four virus strains. The two-dose regimen in vaccine-unprimed participants (Group 1) achieved similar SPRs in comparison with the one-dose in the vaccine-primed group (Group 3), and the SPRs in Group 1 and Group 3 were higher in vaccine-unprimed participants of the one-dose regimen (Group 2). The present study supports the recommendations of a two-dose regimen for IIV4 use in children aged 3-8 years.
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Speckle-type pox virus and zinc finger (POZ) protein (SPOP), a substrate recognition adaptor of cullin-3 (CUL3)/RING-type E3 ligase complex, is investigated for its role in cardiac fibrosis in our study. Cardiac fibroblasts (CFs) activation was achieved with TGF-ß1 (20 ng/mL) and MI mouse model was established by ligation of the left anterior descending coronary, and lentivirus was employed to mediate interference of SPOP expression. SPOP was increased both in fibrotic post-MI mouse hearts and TGF-ß1-treated CFs. The gain-of-function of SPOP promoted myofibroblast transformation in CFs, and exacerbated cardiac fibrosis and cardiac dysfunction in MI mice, while the loss-of-function of SPOP exhibited the opposite effects. Mechanistically, SPOP bound to the receptor of activated protein C kinase 1 (RACK1) and induced its ubiquitination and degradation by recognizing Ser/Thr-rich motifs on RACK1, leading to Smad3-mediated activation of CFs. Forced RACK1 expression canceled the effects of SPOP on cardiac fibrosis. The study reveals therapeutic targets for fibrosis-related cardiac diseases.
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Infarto do Miocárdio , Fator de Crescimento Transformador beta1 , Animais , Camundongos , Fibrose , Infarto do Miocárdio/complicações , Infarto do Miocárdio/metabolismo , Proteínas Nucleares/genética , Proteínas Nucleares/metabolismo , Receptores de Quinase C Ativada , Fator de Crescimento Transformador beta1/metabolismo , Fator de Crescimento Transformador beta1/farmacologiaRESUMO
In real applications, several unpredictable or uncertain factors could result in unpaired multiview data, i.e., the observed samples between views cannot be matched. Since joint clustering among views is more effective than individual clustering in each view, we investigate unpaired multiview clustering (UMC), which is a valuable but insufficiently studied problem. Due to lack of matched samples between views, we could fail to build the connection between views. Therefore, we aim to learn the latent subspace shared by views. However, existing multiview subspace learning methods usually rely on the matched samples between views. To address this issue, we propose an iterative multiview subspace learning strategy iterative unpaired multiview clustering (IUMC), aiming to learn a complete and consistent subspace representation among views for UMC. Moreover, based on IUMC, we design two effective UMC methods: 1) Iterative unpaired multiview clustering via covariance matrix alignment (IUMC-CA) that further aligns the covariance matrix of subspace representations and then performs clustering on the subspace and 2) iterative unpaired multiview clustering via one-stage clustering assignments (IUMC-CY) that performs one-stage multiview clustering (MVC) by replacing the subspace representations with clustering assignments. Extensive experiments show the excellent performance of our methods for UMC, compared with the state-of-the-art methods. Also, the clustering performance of observed samples in each view can be considerably improved by those observed samples from the other views. In addition, our methods have good applicability in incomplete MVC.
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Two novel compounds including a cyclohelminthol type polyketide (namely oxaleimide K, 1) and a maleimide derivative (namely peniroquefortine A, 2), and a new natural product (namely 2-(acetylamino)-N-[(1E)-2-phenylethenyl]-acetamide, 3), together with four known compounds (4-7), were isolated and identified from fungus Penicillium roqueforti, which was separated from the root soil of Hypericum beanii N. Robson collected from the Shennongjia Forestry District, Hubei Province. Their structures including absolute configurations were mainly established by the NMR spectroscopy analyses and single-crystal X-ray diffraction experiment. Compound 1 represents the second example of a cyclohelminthol type polyketide, which features a rare 6/6/5/5 tetracyclic system and a branched aliphatic chain containing a terminal olefin (oct-1-en-3-yl) moiety, and compound 2 possesses an unprecedented carbon skeleton that is uniquely defined by a maleimide moiety linked to the respective 4-methylene-2-(3-methylbut-2-en-1-yl)-phenol and para-substituted aromatic moieties via the carbon-carbon bonds. Remarkably, the absolute configuration of a cyclohelminthol type polyketide as exemplified by compound 1 is determined by the single-crystal diffraction analysis for the first time, highlighting an E-configuration for the linkage of a succinimide moiety and a tetrahydrofuran moiety for 1 rather than a Z-configuration as previously reported in the biosynthesis study, which gives a new insight into the structural elucidation of this category of polyketides. Additionally, compound 1 exhibited significant cytotoxic activity against multiple tumor cells, especially against the Farage and SU-DHL-2 cells (IC50 < 20 µM, 48 h). Further mechanism study revealed that compound 1 significantly induced cell cycle arrest in Farage and SU-DHL-2 cells by causing abnormal ROS level and triggering oxidative stress.
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The present study investigated the chemical constituents from the leaves of Craibiodendron yunnanense. The compounds were isolated and purified from the leaves of C. yunnanense by a combination of various chromatographic techniques including column chromatography over polyamide, silica gel, Sephadex LH-20, and reversed-phase HPLC. Their structures were identified by extensive spectroscopic analyses including MS and NMR data. As a result, 10 compounds, including melionoside F(1), meliosmaionol D(2), naringenin(3), quercetin-3-O-α-L-arabinopyranoside(4), epicatechin(5), quercetin-3'-glucoside(6), corbulain Ib(7), loliolide(8), asiatic acid(9), and ursolic acid(10), were isolated. Compounds 1 and 2 were two new compounds, and compound 7 was isolated from this genus for the first time. All compounds showed no significant cytotoxic activity by MTT assay.
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Catequina , Ericaceae , Quercetina , Folhas de Planta , Cromatografia Líquida de Alta PressãoRESUMO
Tutin, an established toxic natural product that causes epilepsy in rodents, is often used as a tool to develop animal model of acute epileptic seizures. However, the molecular target and toxic mechanism of tutin were unclear. In this study, for the first time, we conducted experiments to clarify the targets in tutin-induced epilepsy using thermal proteome profiling. Our studies showed that calcineurin (CN) was a target of tutin, and that tutin activated CN, leading to seizures. Binding site studies further established that tutin bound within the active site of CN catalytic subunit. CN inhibitor and calcineurin A (CNA) knockdown experiments in vivo proved that tutin induced epilepsy by activating CN, and produced obvious nerve damage. Together, these findings revealed that tutin caused epileptic seizures by activating CN. Moreover, further mechanism studies found that N-methyl-D-aspartate (NMDA) receptors, gamma-aminobutyric acid (GABA) receptors and voltage- and Ca2+- activated K+ (BK) channels might be involved in related signaling pathways. Our study fully explains the convulsive mechanism of tutin, which provides new ideas for epilepsy treatment and drug development.
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Calcineurina , Epilepsia , Animais , Camundongos , Calcineurina/genética , Calcineurina/metabolismo , Epilepsia/induzido quimicamente , Epilepsia/tratamento farmacológico , Epilepsia/genética , Picrotoxina , Receptores de GABA/metabolismo , Receptores de N-Metil-D-Aspartato , Convulsões/induzido quimicamente , Convulsões/genéticaRESUMO
Blockade of programmed cell death 1 (PD-1)/programmed cell death 1 ligand (PD-L1) has evolved into one of the most promising immunotherapy strategies for cancer patients. Tumor cells frequently overexpress PD-L1 to evade T cell-mediated immune surveillance. However, the specific genetic alterations that drive aberrant overexpression of PD-L1 in cancer cells remain poorly understood. The gene encoding the E3 ubiquitin ligase substrate-binding adaptor SPOP is frequently mutated in endometrial cancer (EC). Here, we report that SPOP negatively regulates PD-L1 expression at the transcriptional level. Wild-type SPOP binds to IRF1, a primary transcription factor responsible for the inducible expression of PD-L1, and subsequently triggers its ubiquitin- proteasomal degradation to suppress IRF1-mediated transcriptional upregulation of PD-L1. In contrast, EC-associated SPOP mutants lose their capacity to degrade IRF1 but stabilize IRF1, and upregulate PD-L1 expression. EC-associated SPOP mutations accelerate xenograft tumor growth partially by increasing IRF1 and PD-L1 expression. Together, we identify SPOP as a negative regulator of the IRF1-PD-L1 axis and characterize the critical roles of IRF1 and PD-L1 in SPOP mutation-driven tumor immune evasion in EC.
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Neoplasias do Endométrio , Proteínas Nucleares , Evasão Tumoral , Feminino , Humanos , Antígeno B7-H1/genética , Antígeno B7-H1/metabolismo , Linhagem Celular Tumoral , Neoplasias do Endométrio/genética , Fator Regulador 1 de Interferon/genética , Fator Regulador 1 de Interferon/metabolismo , Mutação/genética , Proteínas Nucleares/metabolismoRESUMO
ETHNOPHARMACOLOGICAL RELEVANCE: The plant Erigeron breviscapus (Vant.) Hand.-Mazz.,a Chinese herbal medicine with multiple pharmacological effects and clinical applications, has been traditionally used in the treatment of paralysis caused by stroke and joint pain from rheumatism by the Yi minority people of Southwest China for generations.However, its mechanism involves many factors and has not been fully clarified. AIM OF THE STUDY: Taking intestinal flora as the target, the protective effect of extract(breviscapine) of E. breviscapus on cerebral ischemia and its possible mechanism were discussed from the perspective of brain inflammatory pathway and intestinal CYP3A4, which depends on intestinal flora. MATERIALS AND METHODS: In this study, we first verified the binding ability between major active ingredient of Erigeron breviscapus and the core target TLR4 protein by molecular docking using Vina software.We established a rat model of cerebral ischemia-reperfusion injury in vivo.The neurological function of rats was scored by Bederson score table, the cerebral infarction volume was detected by TTC staining, and the serum NSE level was detected by ELASA. 16S rRNA sequencing was used to detect the intestinal flora of rats in each group.The expression levels of cerebral TLR4/MyD88/NF-κB and CYP3A4 mRNA and protein in different intestinal segments were detected by qRT-PCR and Western blot. RESULTS: Compared with the model group, the neurological injury score, infarct volume and serum NSE concentration of breviscapine low, medium and high dose groups and nimodipine groups decreased significantly. Meanwhile, breviscapine could significantly reduce the expression level of the TLR4/MyD88/NF-κB in brain tissue and CYP3A4 in different intestinal segments of rats with cerebral ischemia-reperfusion injury. In addition, breviscapine also significantly ameliorated intestinal flora dysbiosis of rats with cerebral ischemia-reperfusion injury. CONCLUSIONS: Breviscapine can protect rats from cerebral ischemia-reperfusion injury by regulating intestinal flora, inhibiting brain TLR4/MyD88/NF-κB inflammatory pathway and intestinal CYP3A4 expression.
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Isquemia Encefálica , Medicamentos de Ervas Chinesas , Erigeron , Microbioma Gastrointestinal , Traumatismo por Reperfusão , Animais , Isquemia Encefálica/tratamento farmacológico , Isquemia Encefálica/metabolismo , Citocromo P-450 CYP3A/genética , Citocromo P-450 CYP3A/metabolismo , Medicamentos de Ervas Chinesas/farmacologia , Medicamentos de Ervas Chinesas/uso terapêutico , Erigeron/genética , Erigeron/metabolismo , Flavonoides , Simulação de Acoplamento Molecular , Fator 88 de Diferenciação Mieloide/genética , Fator 88 de Diferenciação Mieloide/metabolismo , NF-kappa B/metabolismo , Nimodipina/farmacologia , RNA Mensageiro/metabolismo , RNA Ribossômico 16S , Ratos , Ratos Sprague-Dawley , Traumatismo por Reperfusão/tratamento farmacológico , Traumatismo por Reperfusão/metabolismo , Transdução de Sinais , Receptor 4 Toll-Like/genética , Receptor 4 Toll-Like/metabolismoRESUMO
One new taraxastane-type triterpenoid, three new grayanane-type diterpenoids (2 - 4), and 12 known compounds (5 - 16) were isolated from the leaves of Craiobiodendron yunnanens W. W. Smith. The structures of these compounds were elucidated on the basis of their spectroscopic data and chemical evidence. Compounds 1 and 8 exhibited partly anti-inflammatory activity based on the inhibition of NF-κB activity in SW480 cells at 10 µM with inhibition ratios of 60.53 and 59.20%, respectively. Compounds 10 and 13 showed excellent cytotoxicity against human leukemia cell (MV4-11) at 10 µM with inhibition ratios of 43.02 and 49.11%, respectively.
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Diterpenos , Ericaceae , Humanos , Terpenos/farmacologia , Estrutura Molecular , Ericaceae/química , Folhas de Planta/química , Diterpenos/farmacologia , Diterpenos/químicaRESUMO
Six undescribed caryophyllene sesquiterpenoids named pestalotiopsins O-T, along with eight known analogues, were obtained from the fungus Pestalotiopsis chamaeropis. Their structures and absolute configurations were assigned by NMR spectroscopic analyses, HRESIMS, single-crystal X-ray diffraction, electronic circular dichroism (ECD) calculations, Mo2(OAc)4-induced ECD, and chemical derivatization. Pestalotiopsin P represents the first example of a caryophyllene sesquiterpenoid possessing an oxatricyclo [7.2.2.03.6]tridecane decorated with a rare bridgehead double bond, while pestalotiopsin Q has an oxatricyclic [6.3.1.01,4]dodecane skeleton with an unusual ether bridge between C-1 and C-5. These undescribed caryophyllene sesquiterpenoids were screened for their cytotoxic and anti-inflammatory activities.
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Sesquiterpenos , Estrutura Molecular , Sesquiterpenos/farmacologia , Sesquiterpenos/química , FungosRESUMO
BACKGROUND: The gene encoding the E3 ubiquitin ligase substrate-binding adapter Speckle-type BTB/POZ protein (SPOP) is frequently mutated in prostate cancer (PCa) and endometrial cancer (EC); however, the molecular mechanisms underlying the contribution of SPOP mutations to tumorigenesis remain poorly understood. METHODS: BRAF harbors a potential SPOP-binding consensus motif (SBC) motif. Co-immunoprecipitation assays demonstrated that BRAF interacts with SPOP. A series of functional analyses in cell lines were performed to investigate the biological significance of MAPK/ERK activation caused by SPOP mutations. RESULTS: Cytoplasmic SPOP binds to and induces non-degradative ubiquitination of BRAF, thereby reducing the interaction between BRAF and other core components of the MAPK/ERK pathway. SPOP ablation increased MAPK/ERK activation. EC- or PCa-associated SPOP mutants showed a reduced capacity to bind and ubiquitinate BRAF. Moreover, cancer-associated BRAF mutations disrupted the BRAF-SPOP interaction and allowed BRAF to evade SPOP-mediated ubiquitination, thereby upregulating MAPK/ERK signaling and enhancing the neoplastic phenotypes of cancer cells. CONCLUSIONS: Our findings provide new insights into the molecular link between SPOP mutation-driven tumorigenesis and aberrant BRAF-dependent activation of the MAPK/ERK pathway.
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Heavy metal concentration is an important index for evaluating soil pollution. It is of great significance to measure the trace element content accurately for green agriculture development. In order to detect the trace element content accurately, a new prediction framework including pre-processing, signal extraction, feature selection and decision-making was proposed. The energy dispersive X-ray fluorescence (ED-XRF) spectra of 57 national standard soil samples were investigated based on the proposed methods. Firstly, an innovative background deduction method called iterative adaptive window empirical wavelet transform (IAWEWT) was introduced to extract effective counts of characteristic peaks, and the proposed approach was validated by the coefficient of determination (R2) of the instrumental calibration curve compared with two other conventional methods. Secondly, principal component analysis (PCA) was combined with the analysis of variance (ANOVA) for variable selection optimization of the ED-XRF spectrum. After PCA feature extraction and ANOVA variable selection treatment, the optimum number of principal components for V, Cr, Cu, Zn, Mo, Cd and Pb were determined to be 7, 15, 4, 4, 4, 5 and 12 respectively. Furthermore, the support vector regression (SVR) model was adopted for heavy metal estimation. The evaluation indices included R2 and root mean square error (RMSE). It was demonstrated that the predictive capabilities of seven heavy metal elements were improved substantially for elemental analysis by the proposed PCA-ANOVA-SVR model, with excellent results for V, Cr, Cu, Zn, Mo, Cd and Pb estimates, and the R2 values were 0.993, 0.996, 0.999, 0.999, 0.997, 0.998 and 0.998 respectively. Therefore, the new framework proposed in this paper can effectively eliminate redundant features and determine the concentration of trace elements in soil. It provides an effective alternative for the quantitative analysis of X-ray fluorescence spectrometry.
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Metais Pesados , Poluentes do Solo , Oligoelementos , Solo/química , Oligoelementos/análise , Poluentes do Solo/análise , Análise de Componente Principal , Raios X , Cádmio/análise , Chumbo/análise , Monitoramento Ambiental/métodos , Metais Pesados/análise , Análise de VariânciaRESUMO
A pair of stilbenes with γ-lactam unit [(+)-1 and (-)-1], a new phenolic glucoside (2), and a new isoflavone glucoside (3), together with two known compounds (4-5) were isolated from the rhizomes of Belamcanda chinensis. The chemical structures of the undescribed compounds were elucidated on the basis of detailed spectroscopic analyses. Compounds 1, 4, and 5 (10 µM) exhibited anti-inflammatory activities with inhibition rates of 30.46%, 60.34%, and 37.91%, respectively, against the NF-κB signaling pathway.