Shot-Noise Limited Nonlinear Optical Imaging Excited With GHz Femtosecond Pulses and Denoised by Deep-Learning.

Multiphoton fluorescence microscopy excited with femtosecond pulses at high repetition rates, particularly in the range of 100's MHz to GHz, offers an alternative solution to suppress photoinduced damage to biological samples, for example, photobleaching. Here, we demonstrate the use of a U-Net-based deep-learning algorithm for suppressing the inherent shot noise of the two-photon fluorescence images excited with GHz femtosecond pulses. With the trained denoising neural network, the image quality of the representative two-photon fluorescence images of the biological samples is shown to be significantly improved. Moreover, for input raw images with even SNR reduced to -4.76 dB, the trained denoising network can recover the main image structure from noise floor with acceptable fidelity and spatial resolution. It is anticipated that the combination of GHz femtosecond pulses and deep-learning denoising algorithm can be a promising solution for eliminating the trade-off between photoinduced damage and image quality in nonlinear optical imaging platforms.

Dynamic gain driven mode-locking in GHz fiber laser.

Ultrafast lasers have become powerful tools in various fields, and increasing their fundamental repetition rates to the gigahertz (GHz) level holds great potential for frontier scientific and industrial applications. Among various schemes, passive mode-locking in ultrashort-cavity fiber laser is promising for generating GHz ultrashort pulses (typically solitons), for its simplicity and robustness. However, its pulse energy is far lower than the critical value of the existing theory, leading to open questions on the mode-locking mechanism of GHz fiber lasers. Here, we study the passive mode-locking in GHz fiber lasers by exploring dynamic gain depletion and recovery (GDR) effect, and establish a theoretical model for comprehensively understanding its low-threshold mode-locking mechanism with multi-GHz fundamental repetition rates. Specifically, the GDR effect yields an effective interaction force and thereby binds multi-GHz solitons to form a counterpart of soliton crystals. It is found that the resulting collective behavior of the solitons effectively reduces the saturation energy of the gain fiber and permits orders of magnitude lower pulse energy for continuous-wave mode-locking (CWML). A new concept of quasi-single soliton defined in a strongly correlated length is also proposed to gain insight into the dynamics of soliton assembling, which enables the crossover from the present mode-locking theory to the existing one. Specifically, two distinguishing dynamics of Q-switched mode-locking that respectively exhibit rectangular- and Gaussian-shape envelopes are theoretically indicated and experimentally verified in the mode-locked GHz fiber laser through the measurements using both the standard real-time oscilloscope and emerging time-lens magnification. Based on the proposed criterion of CWML, we finally implement a GDR-mediated mode-locked fiber laser with an unprecedentedly high fundamental repetition rate of up to 21 GHz and a signal-to-noise ratio of 85.9 dB.

Effect of dietary fibre on cognitive function and mental health in children and adolescents: a systematic review and meta-analysis.

Objective: The purpose of this study is to investigate the impact of dietary fibre on the mental health and cognitive function of children and adolescents. Methods: All interventional and observational studies that contained information on the relevant population (children and adolescents), intervention/exposures (high dietary fibre consumption) and outcomes (mental and cognitive parameters) were eligible. Eight electronic databases (Embase, Medline, Pubmed, Web of Science, Scopus, PsycINFO, Cochrane Library and ClinicalTrials.gov) were searched up to December 11, 2023. Results: A total of 15 studies (n = 4628) met inclusion criteria, consisting of 9 intervention trials and 6 observational studies. According to observational studies, higher dietary fibre consumption was associated with a 49% reduction in the odds of depression compared to lower intake (P < 0.0001; OR = 0.51; 95% CI: 0.38, 0.69; I2 = 0%). Furthermore, no significant correlations were found between dietary fibre consumption and intelligence or anxiety. Among intervention studies, no significant difference was observed between fibre supplementation and placebo in terms of anxiety (standardized mean difference (SMD): -0.23; 95% CI: -0.72, 0.27), stress (SMD: 0.03; 95% CI: -0.21, 0.28), memory (SMD: 0.46; 95% CI: -0.79, 1.71), or attention (SMD: -2.72; 95% CI: -6.30, 0.86). Conclusion: Evidence from observation studies demonstrated that higher dietary fibre consumption is associated with a decreased odds of depression symptoms, both in childhood and adolescence. However, the causal relationship between dietary fibre intake and mental and cognitive function in children and adolescents still requires further clarification through high-quality intervention studies in the future.

Near-Infrared Mechanoluminescence from Cr3+-Doped Spinel Nanoparticles for Potential Oral Diseases Detection.

Mechanoluminescence (ML) phosphors have found various promising utilizations such as in non-destructive stress sensing, anti-counterfeiting, and bio stress imaging. However, the reported NIR MLs have predominantly been limited to bulky particle size and weak ML intensity, hindering the further practical applications. For this regard, a nano-sized ZnGa2O4: Cr3+ NIR ML phosphor is synthesized by hydrothermal method. By improving the synthesis method and regulating the chemical composition, the NIR ML (600-1000 nm) intensity of such nano-materials has been further enhanced about four times. The reasons for the ML performance difference between micro-/nano- sized phosphors also have been preliminarily analyzed. Additionally, this work probes into the ML mechanism deeply in traps' aspect from band structure and defect formation energy, which can supply significant references for a new approach to develop efficient NIR ML nanoparticles. Finally, due to excellent tissue penetration capability, nano-sized ZnGa2O4:Cr3+ NIR ML phosphor shows great potential applications in biomedical fields such as for the detection of clinical oral diseases.

Dietary fiber for the prevention of childhood obesity: a focus on the involvement of the gut microbiota.

Given the worldwide epidemic of overweight and obesity among children, evidence-based dietary recommendations are fundamentally important for obesity prevention. Although the significance of the human gut microbiome in shaping the physiological effects of diet and obesity has been widely recognized, nutritional therapeutics for the mitigation of pediatric obesity globally are only just starting to leverage advancements in the nutritional microbiology field. In this review, we extracted data from PubMed, EMBASE, Scopus, Web of Science, Google Scholar, CNKI, Cochrane Library and Wiley online library that focuses on the characterization of gut microbiota (including bacteria, fungi, viruses, and archaea) in children with obesity. We further review host-microbe interactions as mechanisms mediating the physiological effects of dietary fibers and how fibers alter the gut microbiota in children with obesity. Contemporary nutritional recommendations for the prevention of pediatric obesity are also discussed from a gut microbiological perspective. Finally, we propose an experimental framework for integrating gut microbiota into nutritional interventions for children with obesity and provide recommendations for the design of future studies on precision nutrition for pediatric obesity.

Transparent nanocrystal-in-glass composite fibers for multifunctional temperature and pressure sensing.

The pursuit of compact and integrated devices has stimulated a growing demand for multifunctional sensors with rapid and accurate responses to various physical parameters, either separately or simultaneously. Fluorescent fiber sensors have the advantages of robust stability, light weight, and compact geometry, enabling real-time and noninvasive signal detection by monitoring the fluorescence parameters. Despite substantial progress in fluorescence sensors, achieving multifunctional sensing in a single optical fiber remains challenging. To solve this problem, in this study, we present a bottom-up strategy to design and fabricate thermally drawn multifunctional fiber sensors by incorporating functional nanocrystals with temperature and pressure fluorescence responses into a transparent glass matrix. To generate the desired nanocrystal-in-glass composite (NGC) fiber, the fluorescent activators, incorporated nanocrystals, glassy core materials, and cladding matrix are rationally designed. Utilizing the fluorescence intensity ratio technique, a self-calibrated fiber sensor is demonstrated, with a bi-functional response to temperature and pressure. For temperature sensing, the NGC fiber exhibits temperature-dependent near-infrared emission at temperatures up to 573 K with a maximum absolute sensitivity of 0.019 K-1. A pressure-dependent upconversion emission is also realized in the visible spectral region, with a linear slope of -0.065. The successful demonstration of multifunctional NGC fiber sensors provides an efficient pathway for new paradigms of multifunctional sensors as well as a versatile strategy for future hybrid fibers with novel combinations of magnetic, optical, and mechanical properties.

Visible-to-Near-Infrared Mechanoluminescence in Bi-Activated Spinel Compounds for Multiple Information Anticounterfeiting.

Mechanoluminescence (ML) is the nonthermal luminescence generated in the process of force-to-light conversion, which has broad prospects in stress sensing, wearable devices, biomechanics, and multiple information anticounterfeiting. Multivalence emitter ions utilize their own self-reduction process to realize multiband ML without introducing another dopant, such as Eu3+/Eu2+, Sm3+/Sm2+, and Mn4+/Mn2+. However, self-reduction-induced ML in bismuth-activated materials has rarely been reported so far. In this work, a novel visible-to-near-infrared (vis-NIR) ML induced by the self-reduction of Bi3+ to Bi2+ in the spinel-type compound (MgGa2O4) is reported. The photoluminescence (PL) spectra, PL excitation (PLE) spectra, and PL lifetime curves demonstrate that Bi3+/Bi2+ ions are the main luminescence centers. Notably, the possible self-reduction model is proposed, where a magnesium vacancy (VMg″) is considered as the driving force for the self-reduction of Bi3+ to Bi2+. Furthermore, an oxygen vacancy (VO••) is confirmed by electron paramagnetic resonance (EPR) spectroscopy. Combined with thermoluminescence (TL) glow curves and ML spectra, a plausible trap-controlled ML mechanism is illustrated, where electron-hole (VO••/VMg″) pairs play a significant role in capturing electrons and holes. It is worth noting that the proof-of-concept dual-mode electronic signature application is implemented based on the flexible ML film, which improves the capabilities of signature anticounterfeiting for high-level security applications. Besides, multistimulus-responsive luminescence behaviors of the ML film are realized under the excitation of a 254 nm UV lamp, thermal disturbance, 980 nm laser, and mechanical stimuli. In general, this study provides new insights into designing vis-NIR ML materials toward wider application possibilities.

Genome-Wide Identification of the Pectate Lyase Gene Family in Potato and Expression Analysis under Salt Stress.

Pectin is a structural polysaccharide and a major component of plant cell walls. Pectate lyases are a class of enzymes that degrade demethylated pectin by cleaving the α-1,4-glycosidic bond, and they play an important role in plant growth and development. Currently, little is known about the PL gene family members and their involvement in salt stress in potato. In this study, we utilized bioinformatics to identify members of the potato pectate lyase gene family and analyzed their gene and amino acid sequence characteristics. The results showed that a total of 27 members of the pectate lyase gene family were identified in potato. Phylogenetic tree analysis revealed that these genes were divided into eight groups. Analysis of their promoters indicated that several members' promoter regions contained a significant number of hormone and stress response elements. Further, we found that several members responded positively to salt treatment under single salt and mixed salt stress. Since StPL18 exhibited a consistent expression pattern under both single and mixed salt stress conditions, its subcellular localization was determined. The results indicated that StPL18 is localized in the endoplasmic reticulum membrane. The results will establish a foundation for analyzing the functions of potato pectate lyase family members and their expression under salt stress.

Boosted Second Harmonic Generation and Cascaded Sum Frequency Generation from a Surface Crystallized Glass Ceramic Microcavity.

The development of novel materials and structures for efficient second-order nonlinear micro/nano devices remains a significant challenge. In this study, the remarkable enhancement of second-harmonic generation (SHG) and cascaded sum frequency generation in whispering gallery mode microspheres made of surface-crystallized glass with a 6-µm Ba2TiSi2O8 crystal layer are demonstrated. Attributed to the core-shell design, the Ba2TiSi2O8 located on the surface can be efficiently coupled with whispering gallery modes, resulting in a highly efficient micron-scale cavity-enhanced second-order optical nonlinearity. Greatly enhanced SHG of the microcavity is observed, which is up to 80 times stronger than that of a non-resonant sample. Furthermore, owing to the wavelength non-selectivity of random quasi-phase matching, ultra-wideband SHG with a strong response ranging from 860 to 1600 nm and high-contrast polarization characteristics is demonstrated. The glass-ceramic-based microsphere cavity also boosts the cascading optical nonlinearity, manifested by a two-magnitude enhancement of cascaded sum frequency generation. This work delineates an efficient strategy for boosting nonlinear optical response in glass ceramics, which will open up new opportunities for applications in photonics and optical communications.

10 W super-wideband ultra-low-intensity-noise single-frequency fiber laser at 1 µm.

A 10 W super-wideband ultra-low-intensity-noise single-frequency fiber laser (SFFL) at 1 µm is experimentally demonstrated, based on dual gain saturation effects from semiconductors and optical fibers, together with an analog-digital hybrid optoelectronic feedback loop. Three intensity-noise-inhibited units synergistically work, which actualizes a connection of effective bandwidth and enhancement of noise-suppressing amplitude. With the cascade action of the semiconductor optical amplifier and optical fiber amplifier, the laser power is remarkably boosted. Eventually, an SFFL with an output power of 10.8 W and a relative intensity noise (RIN) below -150 dB/Hz at the frequency range over 1 Hz is realized. More meaningfully, within the total frequency range of 10 Hz to 10 GHz exceeding 29 octaves, the RIN is controlled to below -160 dB/Hz, approaching the shot-noise limit (SNL) level. To the best of our knowledge, this is the lowest RIN result of SFFL within such an extensive frequency range, and this is the highest output power of the near-SNL super-wideband SFFL. Furthermore, a linewidth of less than 0.8 kHz, a long-term stable polarization extinction ratio of 20 dB, and an optical signal-to-noise ratio of over 60 dB are obtained simultaneously. This start-of-the-art SFFL has provided a systematic solution for high-power and low-noise light sources, which is competitive for sophisticated applications, such as free-space laser communication, space-based gravitational wave detection, and super-long-distance space coherent velocity measurement and ranging.

Watt-level all polarization-maintaining femtosecond fiber laser source at 1100 †nm.

We demonstrate a compact watt-level all polarization-maintaining (PM) femtosecond fiber laser source at 1100 nm. The fiber laser source is seeded by an all PM fiber mode-locked laser employing a nonlinear amplifying loop mirror. The seed laser can generate stable pulses at a fundamental repetition rate of 40.71 MHz with a signal-to-noise rate of >100 dB and an integrated relative intensity noise of only ∼0.061%. After two-stage external amplification and pulse compression, an output power of ∼1.47 W (corresponding to a pulse energy of ∼36.1 nJ) and a pulse duration of ∼251 fs are obtained. The 1100 nm femtosecond fiber laser is then employed as the excitation light source for multicolor multi-photon fluorescence microscopy of Chinese hamster ovary (CHO) cells stably expressing red fluorescent proteins.

Behavioral dynamics of neuroprotective macrophage polarization in neuropathic pain observed by GHz femtosecond laser two-photon excitation microscopy.

Macrophage polarization in neurotoxic (M1) or neuroprotective (M2) phenotypes is known to play a significant role in neuropathic pain, but its behavioral dynamics and underlying mechanism remain largely unknown. Two-photon excitation microscopy (2PEM) is a promising functional imaging tool for investigating the mechanism of cellular behavior, as using near-infrared excitation wavelengths is less subjected to light scattering. However, the higher-order photobleaching effect in 2PEM can seriously hamper its applications to long-term live-cell studies. Here, we demonstrate a GHz femtosecond (fs) 2PEM that enables hours-long live-cell imaging of macrophage behavior with reduced higher-order photobleaching effect-by leveraging the repetition rate of fs pulses according to the fluorescence lifetime of fluorophores. Using this new functional 2PEM platform, we measure the polarization characteristics of macrophages, especially the long-term cellular behavior in efferocytosis, unveiling the dynamic mechanism of neuroprotective macrophage polarization in neuropathic pain. These efforts can create new opportunities for understanding long-term cellular dynamic behavior in neuropathic pain, as well as other neurobiological problems, and thus dissecting the underlying complex pathogenesis.

Frequency-stabilized improvement of saturated absorption spectroscopy based on laser linewidth-control strategy.

Single-frequency fiber lasers (SFFLs), 1083 nm, have been extensively applied in 4He optical pumping magnetometers (OPMs) for magnetic field detection. However, the sensitivity and accuracy of OPMs are constrained by the frequency stability of SFFLs. Focusing on this concern, the frequency-stabilized performance of the 1083 nm SFFLs is successfully improved by externally tailoring the laser linewidth to match the spectral width of the error signal in saturated absorption spectroscopy. Thereinto, a high-intensity error signal of saturated absorption is generated as a large number of 4He atoms with a wide range of velocities interacting with the 1083 nm laser. Consequently, the root mean square value of the fluctuating frequency after locking is effectively decreased from 24.6 to 13.6 kHz, which achieves a performance improvement of 44.7%. Such a strategy can provide a technical underpinning for effectuating an absolute frequency stabilization with higher precision based on atomic and molecular absorption spectroscopy techniques.

Low-noise mode-locking in a GHz repetition rate Tm3+-doped fiber laser.

We demonstrate a GHz repetition rate mode-locked Tm3+-doped fiber laser with low noise. Based on a home-made Tm3+-doped barium gallo-germanate fiber with reduced dispersion, a broad optical spectrum of mode-locking is achieved, and its amplified spontaneous emission quantum-limited timing jitter is largely suppressed. Besides, we carefully investigate the influence of the intracavity pump strength on the noise performance of the mode-locked pulses and find that manipulating the intracavity pump power can be an effective method for optimizing the timing jitter and relative intensity noise (RIN). Particularly, RIN, which originated from the relaxation oscillation, can be effectively suppressed by 33 dB at offset frequencies of >1 MHz. The integrated timing jitter and RIN are only 7.9 fs (10 kHz-10 MHz) and 0.05% (10 Hz-10 MHz), respectively.

1200-W all polarization-maintaining fiber GHz-femtosecond-pulse laser with good beam quality.

In this work, we demonstrate a 1200-W average power all polarization-maintaining (PM) fiber ultrafast laser system operating at 1.0 µm. In accordance with the numerical modeling, the PM fiber laser system is designed and it delivers linearly-polarized femtosecond pulses at a 1.39-GHz fundamental repetition rate, with a maximum output power of 1214 W - to the best of our knowledge, the highest average power from all PM fiber ultrafast laser at 1.0 µm to date. The pulse width can be compressed to ∼800 fs with a beam quality of M2 < 1.1. This kilowatt-class all PM fiber laser system is expected to open new potential for high energy pulse generation through temporal coherent combination and laser ablation using GHz burst fs laser.

Single-frequency fiber laser at 1627†nm based on a self-designed Er-doped hybridized glass fiber.

Aiming at applications like expanding usable wave band of optical telecommunication and preparing Sr optical lattice clocks, a 1627 nm single-frequency fiber laser (SFFL) is demonstrated based on a 7-m-long self-designed Er-doped hybridized glass fiber (EDHF) and a linear cavity configuration with a loop mirror filter (LMF). By inserting a 10-m-long unpumped commercial Er-doped fiber as a dynamic Bragg grating into the LMF, a stable single-longitudinal-mode (SLM) laser with an output power of about 10 mW is obtained. The optical signal-to-noise ratio (OSNR) of SFFL is over 50 dB, and the linewidth is about 3.7 kHz. The measured relative intensity noise (RIN) is less than -140 dB/Hz at frequencies of over 0.5 MHz, and a power variation in 1 h is less than ±0.26%. To our best knowledge, it is the first demonstration of a SFFL operating at the U-band. This 1627 nm SFFL can provide advanced light source technology support for many cutting-edge applications.

Broadband 1.0†µm emission in Nd3+/Yb3+ co-doped phosphate glasses and fibers for photonic applications.

In this work, the spectroscopic properties of 1.0 µm emission in Nd3+/Yb3+ co-doped phosphate glasses were systematically investigated under 808 nm excitation. Notably, broadband 1.0 µm emission with a full width at half maximum (FWHM) of 96 nm was obtained in the phosphate glass doped with 2 mol.% Nd2O3 and 1 mol.% Yb2O3. In addition, the energy transfer microscopic parameter and transfer efficiency were analyzed. What is more, multimaterial fibers with Nd3+/Yb3+ co-doped phosphate glass core and silicate cladding were successfully drawn by using the molten core method. An intense 1.0 µm amplified spontaneous emission (ASE) can be realized in a 3 cm long multimaterial fiber. More importantly, the FWHM of the ASE can reach as large as 60 nm when excited at 976 nm. These results demonstrate that the Nd3+/Yb3+ co-doped phosphate glasses and fibers are promising gain materials for amplifier and laser applications in photonics.

Study on Potato Bud Cultivation Techniques in a Greenhouse in Spring.

The species degeneration caused by traditional potato cultivation methods is becoming increasingly evident, and it is particularly important to study new potato cultivation methods. Sprout planting technology has the advantages of large reproductive capacity, fast growth speed, and simplified maintenance of cultivated crops. In this study, four disease-free potato varieties ('Fujin', 'Youjin', 'Zhongshu 4', and 'Feiwuruita') were treated with different parts (top bud, middle bud, and tail bud) and different bud lengths (10 cm, 15 cm, and 20 cm), and then potato sprout planting was carried out. A nutrient pot experiment was performed following a randomized complete block design (RCBD) with various replicates and a natural control (CK) treatment. By comprehensively measuring the emergence, chlorophyll content, net photosynthetic rate, dry matter distribution during the bulking period of blocks, and effect of growth and quality with bud direct seeding under both treatments, it was found that potato block top bud direct seeding cultivation is significantly superior to other parts. In terms of early maturity and yield statistics, the advantage of top bud cultivation in 'zhongshu 4' is most obvious; it reaches maturity an average of 14 days earlier, and the yield can be increased by 38.05%. Therefore, top bud direct seeding is more suitable for potato sprout planting technology. On this basis, the 20 cm and 15 cm bud length treatments of top buds were used for direct cultivation, and all the above indicators performed well. Among them, in the zhongshu 4 variety, the yields of 15 cm and 20 cm bud length treatments increased by 41.78% and 38.05%, the growth rates of commercial potatoes increased by 6% and 6.9%, respectively, and the effects were the most obvious. In conclusion, the deep research and application of potato sprouting technology has high utilization value for improving potato yield and quality and has guiding significance for greenhouse potato cultivation in early spring.

Broadband near-infrared amplified spontaneous emission of Er3+-doped germanate glass fiber.

Er3+-doped glass and fiber are very attractive for near-infrared (NIR) lasers and photonic applications. In this work, the full width at half maximum (FWHM) of NIR fluorescence emission of the Er3+-doped germanate glass can be broadened from 72 to 99 nm when Al2O3 was added. In addition, the spectroscopic properties, including absorption and emission spectra, Judd-Ofelt intensity parameters, absorption and emission cross sections, gain coefficient, and fluorescence lifetime, of the Al2O3-modified germanate glass were systematically investigated. What is more, silicate-clad heavily Er3+-doped germanate core multimaterial fibers were successfully drawn by a rod-in-tube method. Notably, broadband NIR amplified spontaneous emission (ASE) with an FWHM of 120 nm was achieved in this new fiber. To the best of our knowledge, this is the largest FWHM reported for Er3+-doped germanate glass fibers. These results suggest that the as-drawn Er3+-doped germanate glass fiber with superior performances is a promising candidate for broadband optical amplification.

Ultrafine electro-optical frequency comb based on cascade phase modulation with cyclic frequency shifting.

An ultrafine electro-optical frequency comb (EOFC) with plentiful comb teeth is demonstrated. Adopting a single-frequency fiber laser as a light source, cascade phase modulation based on a sinusoidal signal and a frequency-time transformation (FTT) signal is executed to generate the EOFC with high fineness. Meanwhile, a cyclic fast frequency shifting strategy is introduced to boost the number of comb teeth and the bandwidth of the EOFC. As a result, an EOFC with 12600 comb lines covering a broad bandwidth from -6.3 GHz to 6.3 GHz is established, corresponding to an ultrafine comb space of 1 MHz. Moreover, the power fluctuation of a comb tooth is less than 0.5 dBm. This state-of-the-art EOFC has significant potential in the field of precision spectroscopy.