Ceria Nanoenzyme-Based Hydrogel with Antiglycative and Antioxidative Performance for Infected Diabetic Wound Healing.

Diabetic wound healing still faces a dilemma because of the hostile hyperglycemic, oxidative, and easily-infected wound microenvironment. In addition, advanced glycation end products (AGEs) further impede wound repair by altering the immunological balance. Herein, ceria nanorods with distinctive antiglycative and excellent antioxidative capacities are innovatively introduced into a self-healing and erasable hydrogel, which could reshape the wound microenvironment by expediting hemostasis, inhibiting infection, reducing AGEs, and continuously depleting reactive oxygen species. The remitted oxidative stress and glycosylation synergistically regulate inflammatory responses, and promote revascularization and extracellular matrix deposition, resulting in accelerated diabetic wound repair. This study provides a highly efficient strategy for constructing nanoenzyme-reinforced antiglycative hydrogel that regulates every wound healing stage for diabetic wound management.

Cobalt-Iron Double Ion-Bovine Serum Albumin Chelation-Assisted Thermo-Sensitive Surface-Imprinted Nanocage with High Specificity.

To develop multifunctional protein imprinted materials, a cobalt-iron double ion-BSA directional chelation-assisted thermo-sensitive surface-imprinted hollow nanocage (Co-Fe@CBMA-MIPs) with excellent specificity is developed on the surface of ZIF-67@Co-Fe in this study by synergizing the advantages of surface imprinting, metal ion chelation, anti-protein adsorption segments, and thermo-sensitive components. Beyond previous research, well-designed multifunctional protein-imprinted materials possess high binding capacity, fast adsorption kinetics, and outstanding selectivity. When the adsorption is carried out at 32 °C, the adsorption capacity of Co-Fe@CBMA-MIPs for BSA reaches 520.35 mg/g within 50 min. The imprinting factor is 8.55. The selectivity factors of Co-Fe@CBMA-MIPs for HSA, Bhb, OVA, and Lyz are 3.72, 6.09, 4.10, and 8.41, respectively. More significantly, Co-Fe@CBMA-MIPs could specifically recognize BSA from mixed proteins and actual samples and exhibit excellent repeated use stability. Based on the above advantages, the development of this research provides an effective means to improve the recognition specificity of molecularly imprinted polymers.

Development of surface imprinted heterogeneous nitrogen-doped magnetic carbon nanotubes as promising materials for protein separation and purification.

To promote the development of molecular imprinting technique in the separation and analysis of protein, novel bovine serum albumin (BSA) surface imprinted nitrogen-doped magnetic carbon nanotubes (N-MCNTs@MIPs) are developed by this paper. The imprinted materials are prepared by depositing polydopamine (PDA) on the surface of nitrogen-doped magnetic carbon nanotubes (N-MCNTs). N-MCNTs prepared by high temperature pyrolysis and chemical vapor deposition exhibit high specific surface area, positive hydrophilicity, abundant nitrogen functional groups and excellent magnetic properties. These characteristics are conducive to the increase of effective binding sites, the smooth development of the protein imprinting process in the aqueous phase, the improvement of the binding capacity and the simplification of the separation process. The amount of BSA adsorbing on the N-MCNTs@MIPs can reach 150.86 mg/g within 90 min. The imprinting factor (IF) is 1.43. The results of competitive adsorption and separation of fetal bovine serum showed that N-MCNTs@MIPs can specifically recognize BSA. The excellent reusability and separation ability for real sample prove that N-MCNTs@MIPs have the potential to be applied to the separation and purification of proteins in complex biological samples.

Preparation of BSA surface imprinted manganese dioxide-loaded tubular carbon fibers with excellent specific rebinding to target protein.

Along with the wide development of protein imprinted polymers, the researchers still face many challenges, such as difficult template elution, slow adsorption rate and low adsorption capacity. In order to promote the progress of protein separation and purification, the surface imprinted manganese dioxide-loaded tubular carbon fibers (FTCFs@MnO2@MIPs) are prepared in this work. FTCFs@MnO2@MIPs are based on tubular carbon fibers (TCFs) coated with flaky MnO2. Dopamine (DA) and bovine serum albumin (BSA) are utilized as functional monomers and templates. The MnO2 nanosheets are grown and loaded on the surface of carboxyl-modified tubular carbon fibers (CMTCFs) to form a MnO2 shell, which provides more imprinting sites for protein imprinting. Meanwhile, this shell enhances the interaction between the imprinting sites and BSA. The content of MnO2 loaded on the surface of CMTCFs is 9.42%. The obtained materials are systematically characterized and the adsorption performances of FTCFs@MnO2@MIPs for BSA are investigated. The adsorption process of FTCFs@MnO2@MIPs exhibits significant self-driven characteristics. The adsorption capacity reaches 816.44 mg/g in 60 min and the imprinting factor (IF) is 3.31. FTCFs@MnO2@MIPs can selectively separate BSA from the mixed proteins and fetal bovine serum. Excellent reusability and practical application ability make MnO2-loaded tubular carbon fibers (FTCFs@MnO2) become a promising carrier in the field of protein imprinting.

Preparation of CTCNFs/Co9S8 hybrid nanofibers with enhanced microwave absorption performance.

A three-step synthesis strategy has been applied to the preparation of Co9S8-loaded tubular carbon nanofibers (CTCNFs/Co9S8 hybrid nanofibers) with excellent microwave absorbing ability. Firstly, tubular polymer nanofibers (TPNFs) are synthesized using the confined self-condensation method that we developed. Afterwards, TPNFs are converted into surface carboxylated tubular carbon nanofibers (CTCNFs) by carbonization and subsequent acidification processes. Finally, a hydrothermal method is used for the controllable growth of Co9S8 nanoparticles on CTCNFs, and a series of CTCNFs/Co9S8 hybrid nanofibers with different Co9S8 loading are obtained. The prepared CTCNFs/Co9S8 hybrid nanofibers possess abundant effective interface and defect dipoles, which will lead to stronger polarization. Using the strategy of enhancing dielectric loss, the microwave dissipation ability of CTCNFs/Co9S8 hybrid nanofibers has been significantly improved, showing an excellent low-frequency absorbing performance with a minimum reflection loss of -46.81 dB@5.3 GHz. In addition, the composition, structure and properties of nanofibers have been systematically characterized. The Co9S8 loading on CTCNFs and the filler content of CTCNFs/Co9S8 hybrid nanofibers in matrix are studied and optimized. The microwave attenuation mechanism is also explained.

Preparation of surface protein imprinted thermosensitive polymer monolithic column and its specific adsorption for BSA.

In this work, a novel thermosensitive surface protein imprinted polymer monolithic column (TsIPMC) was synthesized by combining high internal phase emulsion with 1,1-diphenylethene (DPE) controlled polymerization. Innovatively, DPE and acrylic acid (AA) monomers were introduced in high internal oil and water phases respectively. The research showed that DPE could not only initiate the polymerization of monomers, but also improve the pore performance of monolithic columns. The elution efficiency of template or target protein could be significantly improved by the thermoresponse characteristics of TsIPMC. The effects of DPE and AA on adsorption capacity and imprinting factor (IF) were studied. The optimization results presented that the optimal addition amounts were 55 mg and 50 mg. Under such conditions, the IF of as-prepared TsIPMC was 1.61 and the saturated adsorption capacity was 66 mg/mL. The influences of the flow rate and target protein concentration on the adsorption equilibrium time and effluent volume were revealed. TsIPMC showed higher selectivity for different competing proteins. The reuse stability result showed that the adsorption of TsIPMC to BSA decreased by 3.69% after 12 times of reuse, and the IF remained basically unchanged. TsIPMC would demonstrate the potential applications in the field of protein purification and separation.

Surface Microstructure Regulation of Porous Polymer Microspheres by Volume Contraction of Phase Separation Process in Traditional Suspension Polymerization System.

In the present work, the suspension polymerization method is used for the preparation of porous polymer microspheres with different surface morphology, and the preparation mechanism is systematically expounded. The morphology results show that the smooth, convex, and wrinkled microspheres could be controlled by adjusting the ratio of monomer to porogens. The micelles forming the framework support the "Eggshell," and its size and shape directly affect the morphology of "Eggshell." The addition of monomers (GMA), whose polymer has low glass transition temperature (Tg ), is important for the formation of wrinkled morphology. The amount of toluene and polydimethylsiloxane also affects the surface morphology of microspheres. In addition, the effect of polydimethylsiloxane is also more significant. The preparation process of the wrinkled P(GMA-St-EGDA) microspheres with abundant epoxy groups can be amplified. The morphology of the material prepared in the 100 L reactor is well maintained, and the yield in the size range of 80-160 µm is more than 80%. The surface wrinkled porous polymer microspheres have potential applications in the fields of enzyme carrier, separation and purification, and light scattering.