Abscisic acid plays a key role in the mechanism of photosynthetic and physiological response effect of Tetrabromobisphenol A on tobacco.

The toxicity of bromide to animals and microorganisms has been widely studied, but the mechanism by which bromide toxicity affects plants is rarely studied. This study used the bromophenol compound Tetrabromobisphenol A (TBBPA) as a representative of bromide to explore the physiological and molecular response mechanism of tobacco leaves to TBBPA. In addition, physiological determination, transcriptomics, weighted gene co-expression network analysis (WGCNA) analysis, and random forest prediction model were conducted. The findings from this study indicated that TBBPA limited the photoreaction process by destroying the light-catching antenna protein of tobacco leaves, the activity of the photosystem reaction centers (PSII and PSI), and the linear electron transport efficiency. TBBPA also reduced the rate of the Calvin-Benson cycle by inhibiting the activities of gene such as Rubisco, PGK, and TPI, and finally destroyed the photosynthesis process. Although cyclic electron transport was enhanced under stress conditions, it could not reverse the damage caused by TBBPA on photosynthesis. TBBPA exposure resulted in the accumulation of reactive oxygen species (ROS) in tobacco leaves, and the activities of Superoxide dismutase (SOD), Ascorbate peroxidase (APX), and Glutathione peroxidase (GPX) and their coding genes were significantly down-regulated. Although POD activity and proline (Pro) content were increased, they were insufficient to remove excess O2·- free radicals to relieve ROS stress. WCGNA and random forest models predicted that the damage of TBBPA to the above processes in tobacco was closely related to the increase in abscisic acid (ABA) content. TBBPA affects the Calvin cycle by inducing ABA signal transduction and stomatal closure, which leads to a series of chain reactions, such as electron transport chain obstruction, excess of ROS, decrease in chlorophyll synthesis, and photosystem reaction center damage.

Effects of salt concentration, pH, and their interaction on plant growth, nutrient uptake, and photochemistry of alfalfa (Medicago sativa) leaves.

In order to explore the main limiting factors affecting the growth and physiological function of alfalfa under salt and alkali stress, the effect of the salt and alkali stress on the growth and physiological function of alfalfa was studied. The results showed that effects of the excessive salt concentration (100 and 200 mM) on the growth and physiological characteristics were significantly greater than that of pH (7.0 and 9.0). Under 100 mM salt stress, there was no significant difference in the growth and photosynthetic function between pH 9.0 and pH 7.0. Under the 200 mM salt concentration the absorption of Na+ by alfalfa treated at the pH 9.0 did not increase significantly compared with absorption at the pH 7.0. However, the higher pH directly reduced the root activity, leaf's water content, and N-P-K content also decreased significantly. The PSII and PSI activities decreased with increasing the salt concentration, especially the damage degree of PSI. Although the photoinhibition of PSII was not significant, PSII donor and electron transfer from the QA to QB of the PSII receptor sides was inhibited. In a word, alfalfa showed relatively strong salt tolerance capacity, at the 100 mM salt concentration, even when the pH reached 9.0. Thus, the effect on the growth and photosynthetic function was not significant. However, at 200 mM salt concentration, pH 9.0 treatment caused damage to root system and the photosynthetic function in leaves of alfalfa was seriously injured.

Elevated air temperature damage to photosynthetic apparatus alleviated by enhanced cyclic electron flow around photosystem I in tobacco leaves.

High temperature can lead to increased production of excess light energy, thus reducing photosynthetic capacity in plants. Photosynthetic cyclic electron flow (CEF) in photosystem I (PSI) can effectively protect photosystems, but its physiological mechanism under high temperature is poorly understood. In this study, antimycin A (AA) and thenoyltrifluoroacetone (TTFA) were used to inhibit PGR5-and NDH-dependent CEF pathways, respectively, to reveal the photoprotective functions of CEF for PSII in tobacco leaves under high temperature stress (37 °C, HT). High temperatures caused decreases in maximal photochemistry efficiency (Fv/Fm) and damaged photosystem II (PSII) in tobacco leaves. Under AA inhibition of PGR5-dependent CEF, high temperature increased the fluorescence intensity of point O (Fo) in OJIP curves, i.e., the energy absorption per active reaction center (ABS/RC), the trapping rate of the reaction center (TRo/RC), and the electron transport efficiency per reaction center (ETo/RC) in tobacco leaves. High temperature induced an increase in the hydrogen peroxide content and a decrease in pigment content in tobacco leaves. Under the high temperature treatment, inhibition of PGR5-dependent CEF reduced the activities of the PSII reaction center significantly, destroyed the oxygen-evolving complex (OEC), and impeded photosynthetic electron transfer from PSII to the plastoquinone (PQ) pool in tobacco leaves. The TTFA treatment inhibited the NDH-dependent pathway under high temperature conditions, with the relative fluorescence intensity of point I (VI) decreased significantly, and the content of hydrogen peroxide and superoxide anion increased significantly. Additionally, Fo and the redox degree of the PSII donor side (Wk) increased, and pigment content decreased compared to the control, but with little change compared to high temperature treatment, indicating that the inhibition of the NDH-dependent pathway directly weakened the capacity of the PQ pool to lead to the accumulation of reactive oxygen species (ROS) in tobacco leaves. In conclusion, CEF alleviated damage to the photosynthetic apparatus in tobacco leaves by increasing PSII heat dissipation, reducing ROS production, and maintaining the stability of the PQ pool to accommodate photosynthetic electron flow.

Elevated NO2 damages the photosynthetic apparatus by inducing the accumulation of superoxide anions and peroxynitrite in tobacco seedling leaves.

This study aimed to further understand the toxicity of high concentrations of nitrogen dioxide (NO2) to plants, especially to plant photosynthesis. Tobacco plants in the six-leaf stage were exposed to 16.0 µL L-1 NO2 to determine the activities of photosystem II (PSII) and photosystem I (PSI) reaction centers, the blocking site of PSII electron transport, the degree of membrane peroxidation and the relative expression of PsbA, PsbO and PsaA genes in the third fully expanded leaves by using gas exchange and chlorophyll fluorescence techniques, biochemical and RT-PCR analysis. The results showed that 16.0 µL L-1 NO2 caused necrotic lesions to form on leaves and significantly increased the generation rate of superoxide anions (O2-) and the content of peroxynitrite (ONOO-) in leaves of tobacco seedling, leading to damage to cell membrane, chlorophyll content and net photosynthetic rate reduction, and photosynthetic apparatus destruction. Fumigation with 16.0 µL L-1 NO2 decreased the activity of PSII reaction center and oxygen evolution complex, and the relative expression of PabA in leaves of tobacco seedlings to inhibit the electron transport from the donor side to the receptor side of PSII, especially blocking the electron transport from QA to QB on the receptor side. The activity of the PSI reaction center and the relative expression of PsaA decreased, weakening the ability to accept electrons and inhibiting the electron transfer from PSII to PSI, which further increased the damage of PSII of tobacco seedling leaves caused by 16.0 µL L-1 NO2. Therefore, 16.0 µL L-1 NO2 leaded to the accumulation of O2- and ONOO-, which damaged the cell membrane and thylakoid membrane, inhibit the electron transport, and destroyed the photosynthetic apparatus in leaves of tobacco seedlings. The results from this study emphasized the importance of reducing the NO2 concentration in the atmosphere.

Chlorophyll synthesis and the photoprotective mechanism in leaves of mulberry (Morus alba L.) seedlings under NaCl and NaHCO3 stress revealed by TMT-based proteomics analyses.

Chlorophyll (Chl) and effective photoprotective mechanism are important prerequisites to ensure the photosynthetic function of plants under stress. In this study, the effects of 100 mmol L-1 NaCl and NaHCO3 stress on chlorophyll synthesis and photosynthetic function of mulberry seedlings were studied by physiological combined with proteomics technology. The results show that: NaCl stress had little effect on the expression of Chl synthesis related proteins, and there were no significant changes in Chl content and Chl a:b ratio. However, 13 of the 15 key proteins in the process of Chl synthesis were significantly decreased under NaHCO3 stress, and the contents of Chl a and Chl b were significantly decreased (especially Chl a). Although stomatal conductance (Gs) decreased significantly under NaCl stress, net photosynthetic rate (Pn), PSII maximum photochemical efficiency (Fv/Fm) and electron transfer rate (ETR) did not change significantly, but under NaHCO3 stress, not only Gs decreased significantly, PSII activity and photosynthetic carbon were the same. In the photoprotective mechanism under NaCl stress, NAD(P)H dehydrogenase (NDH)-dependent cyclic electron flow (CEF) enhanced, the expression of related proteins subunit, ndhH, ndhI, ndhK, and ndhM, the key enzyme of the xanthophyll cycle, violaxanthin de-epoxidase (VDE) were up-regulated, the ratio of (A + Z)/(V + A + Z) and non-photochemical quenching (NPQ) was increased. The expressions of proteins FTR and Fd-NiR were also significant up-regulated under NaCl stress, Fd-dependent ROS metabolism and nitrogen metabolism can effectively reduce the electronic pressure on Fd. Under NaHCO3 stress, the expressions of NDH-dependent CEF related proteins subunit (ndhH, ndhI, ndhK, ndhM and ndhN), VDE, ZE, FTR, Fd-NiR and Fd-GOGAT, were significant down-regulated, and ZE, CP26, ndhK, ndhM, Fd-NiR, Fd-GOGAT and FTR genes expression also significantly decreased, the photoprotective mechanism, like the xanthophyll cycle，CEF and Fd-dependent ROS metabolism and nitrogen metabolism might be damaged, resulting in the inhibition of PSII electron transfer and carbon assimilation in mulberry leaves under NaHCO3 stress.