RESUMO
The Michael addition of fluorouracil (0.01 mol), uracil (0.01 mol), thymine (0.01 mol) to disaccharide acrylates (0.03 mol) was carried out in pyridine (20 ml) at 50 degrees C for 72 h with lipase M from Mucor javanicus (200 mg). Six new pyrimidine derivatives containing a branched sugar were obtained in yields from 56 to 75%.
Assuntos
Acrilatos/química , Dissacarídeos/química , Fluoruracila/química , Lipase/metabolismo , Mucor/enzimologia , Timina/química , Uracila/químicaRESUMO
Combined regioselective acylation/Michael addition reaction catalyzed by alkaline protease from Bacillus subtilis in anhydrous pyridine for synthesis of N-substituted imidazole derivatives containing a glucose branch via a novel single-enzyme, two-step, one-pot procedure is reported.
Assuntos
Glucose/química , Imidazóis/síntese química , Acilação , Bacillus subtilis/enzimologia , Proteínas de Bactérias/química , Proteínas de Bactérias/farmacologia , Catálise , Endopeptidases/química , Endopeptidases/farmacologia , Modelos Químicos , Piridinas/química , EstereoisomerismoRESUMO
A new activity of alkaline protease from Bacillus subtilis for Michael addition reactions of imidazole, 4-nitro-1H-imidazole and 2-methyl-4-nitro-1H-imidazole with acrylates and acrylic acid was investigated. The reactions were carried out in pyridine at 50 degrees C for 72 h. Five N-substituted imidazole derivatives were obtained using acrylate esters, but not acrylic acid, in yields from 62% to 76%.