Estimation of gestational age in buffaloes (Bubalus bubalis) by transabdominal and transrectal ultrasonography.

The aims of the presented study were (1) to examine the relationship between foetal measurements and gestational age (GA), (2) to generate GA formulas, and (3) to investigate the estimation of GA by transabdominal ultrasonography in buffaloes. Thirteen pregnant buffaloes were used in the study. Transrectal ultrasonography was performed between Day (D) 28 and 112 of gestational age, whereas transabdominal ultrasonography was between 126 and 294. The diameters of embryonic (EVD) and amniotic (AVD) vesicles, crown-rump length (CRL), occipito-nasal length (ONL), biparietal diameter (BPD), orbit diameter (OD), cervical, thoracic, lumbar and coccygeal vertebrae lengths (CVL, TVL, LVL, CcVL), abdominal diameter (AD), chest diameter (CD), umbilical cord diameter (UCD), scapula, humerus, radius-ulna, metacarpus, femur, tibia, and metatarsus lengths (SL, HL, RUL, McL, FL, TL, MtL), diameters of transversal heart (THD), stomach (SD), kidney (KD), and outer diameter, circumference and area of placentomas (OPD, OPC, OPA) were measured by ultrasonography. All 26 parameters were highly correlated with GA (r = 0.968 - 0.999). The observation of the foetus was evident in all animals via transabdominal ultrasonography, and all parameters except EVD, AVD, and CRL could be measured on D 126. In addition, heartbeats, the sign of foetal vitality, could be observed in 11 of 13 living foetuses. This study is the first to demonstrate that transabdominal ultrasonography can be used to estimate GA in buffaloes. In addition, GA formulas related to ONL, CVL, TVL, LVL, CcVL, extremity bone lengths, THD, UCD, PC, and PA measurements were created for the first time in buffaloes.

The comparative results of myostatin introgression from donor Texel to recipient Ramliç sheep with the aspects of growth, pre-, and post-slaughter carcass traits in the second backcross generation.

The aim of the study was to evaluate the growth, body and ultrasonic measurements and some carcass traits of purebred Ramliç, Texel and crossbred (87.5 % Ramliç and 12.5 % Texel) lambs in a marker-assisted introgression (MAI) project. The effects of some environmental factors such as genotype, sex, birth type, dam age, weaning age, and birth weight on growth, ultrasound measurements, and carcass traits were significant ( P < 0.05 ). There were no significant differences between pure Ramliç and its myostatin carrier ( + / - ) and non-carrier ( - / - ) second backcrosses (BC 2 ) for birth weight, daily live weight gain, and weaning weight. The BC 2 genotype ( + / - ) was statistically caught up with pure Ramliç for wither height, body length, and rump width. Differences in the longissimus muscle depth indicated that the BC 2 ( + / - ) genotypes tended to be better for both pure Ramliç and Texel lambs. Texel lambs were superior to Ramliç in the aspect of some carcass characteristics such as leg length, cold right-half carcass weight, foreleg weight in the left-half carcass, and muscle weight in the left leg. BC 2 ( + / - ) lambs were in the middle of both breeds for the traits. BC 2 lambs carrying myostatin did not vary from Ramliç morphologically. The new type of Ramliç was also closer to Texel in the aspect of carcass characteristics. In this connection, improvement of indigenous breeds could be achieved by MAI without changing the essential characteristics. For the summary, please visit http://www.mustafatekerli.com (last access: 14 June 2022).

Teat characteristics in relation to animal temperament during milking in buffaloes, and comparison of buffalo and cow teat morphology.

This study aimed to examine the teat characteristics in relation to the animal temperament during milking in the Anatolian buffaloes using ultrasonographic, histomorphological and immunohistochemical methods. The teat canal length (TCL), teat wall thickness (TWT), teat cisternal diameter (TCD), teat diameter (TD), teat length (TL), and teat circumference (TC) values in docile (n = 5) and nervous (n = 7) buffaloes were measured at the 0th, 3rd and 6th minute of stimulation. In additional experiments, comparative histomorphology and immunohistochemical examinations of buffalo (n = 7) and cow teats (n = 8) were performed. It was determined that post-stimulation mean TCL values were significantly higher in nervous buffaloes than those of teats in docile buffaloes (p < .05). A significant positive correlation between TCD and TD, TL and TC in both docile and nervous buffaloes was noted (p < .05). Unlike nervous buffaloes where only 3/14 teat canals were open by 3rd minute of milking stimulation, almost all (9/10) teat canals were observed opened in docile buffaloes. There were fewer muscle but more collagen bundles in buffalo teats compared with cow teats. It seems that temperament of animal during milking effects the milking efficiency, and in nervous buffaloes, probably the stimulation alone may not be sufficient for opening of the teat canal and hence achieve complete milking.

Ultrasonographic foetometry and maternal serum progesterone concentrations during pregnancy in Turkish Saanen goats.

The objectives of the present study were (1) to generate reliable equations to estimate the gestational age of Saanen goats by measuring embryonic and foetal structures via B-Mode ultrasonography (USG), (2) determine the maternal serum progesterone (P4) concentrations during pregnancy, and (3) compare the relationship between P4 concentrations and gestational age, litter size, and birth weight of kids. Seven pregnant goats were used in this study. Ultrasonographic examinations were performed transrectally from Days 15 to 27 of pregnancy at 3-day intervals and transabdominally from Days 30 to 128 at 7-day intervals. Blood samples were collected daily between Day 15 and 24 of gestation, and weekly from Day 27 to 128 to determine the serum P4 concentrations. Outer placentome diameter, biparietal diameter, occipito-nasal length, chest diameter, foetal heart rate, transversal heart diameter, orbit diameter, umbilical cord diameter, and kidney diameter were highly correlated with gestational age (P < 0.0001). Goats with twins had greater progesterone concentrations than those with a single foetus (P < 0.001). Furthermore, maternal serum progesterone concentrations detected on Days 51, 107, 114, and 128 of gestation were greater in goats carrying twins than those with a single foetus (P < 0.05). A negative correlation was evident between serum progesterone concentrations and birth weights of the kids (r = -0.78; P = 0.007). In conclusion, gestational age can be estimated using ultrasonographic foetometry, and litter size at specific times during pregnancy can be determined based on maternal serum progesterone concentration in Turkish Saanen goats.