A new mechanism of antibody diversity: formation of the natural antibodies containing LAIR1 and LILRB1 extracellular domains.

The recent discovery of public antibodies targeting Plasmodium falciparum-encoded repetitive interspersed families of polypeptides (RIFINs), which contain extracellular immunoglobulin-like domains from LAIR1 or LILRB1, constitutes a significant step forward in comprehending the reactivity of the Plasmodium parasite. These antibodies arise from unique B cell clones and demonstrate extensive cross-reactivity through their interaction with P. falciparum RIFINs. LAIR1 and LILRBs are specialized type I transmembrane glycoproteins, classified as immune inhibitory receptors, restricted to primates and mainly found on hematopoietic cells. They are instrumental in modulating interactions within the tumor microenvironment and across the immune system, and are increasingly recognized as important in anti-cancer immunotherapy and pathogen defense. The presence of LAIR1/LILRB1-containing antibodies offers new insights into malaria parasite evasion strategies and the immune system's response. Additionally, the innovative method of integrating extra exons into the antibody switch region is a noteworthy advancement, enriching the strategies for the generation of a varied array of bispecific and multispecific antibodies.

A strategy to build a library of oil tracers by oleophilic silica-encapsulated DNA nanoparticles.

Artificially synthesized DNA is involved in the construction of a library of oil tracers due to their unlimited number and no-biological toxicity. The strategy of the construction is proposed by oleophilic Silica-encapsulated DNA nanoparticles, which offers fresh thinking in developing novel tracers, sensors, and molecular machines in engineering & applied sciences based on artificially synthesized DNA blocks.

A Dual-domain Engineered Antibody for Efficient HBV Suppression and Immune Responses Restoration.

Chronic hepatitis B (CHB) remains a major public health concern because of the inefficiency of currently approved therapies in clearing the hepatitis B surface antigen (HBsAg). Antibody-based regimens have demonstrated potency regarding virus neutralization and HBsAg clearance. However, high dosages or frequent dosing are required for virologic control. In this study, a dual-domain-engineered anti-hepatitis B virus (HBV) therapeutic antibody 73-DY is developed that exhibits significantly improved efficacy regarding both serum and intrahepatic viral clearance. In HBV-tolerant mice, administration of a single dose of 73-DY at 2 mg kg-1 is sufficient to reduce serum HBsAg by over 3 log10 IU mL-1 and suppress HBsAg to < 100 IU mL-1 for two weeks, demonstrating a dose-lowering advantage of at least tenfold. Furthermore, 10 mg kg-1 of 73-DY sustainably suppressed serum viral levels to undetectable levels for ≈ 2 weeks. Molecular analyses indicate that the improved efficacy exhibited by 73-DY is attributable to the synergy between fragment antigen binding (Fab) and fragment crystallizable (Fc) engineering, which conferred sustained viral suppression and robust viral eradication, respectively. Long-term immunotherapy with reverse chimeric 73-DY facilitated the restoration of anti-HBV immune responses. This study provides a foundation for the development of next-generation antibody-based CHB therapies.

Study on the Coupling Coordination between New-Type Urbanization and Water Ecological Environment and Its Driving Factors: Evidence from Jiangxi Province, China.

With the rapid development of urbanization, problems such as the degradation of water ecological environment have emerged. How to improve the water ecological environment in the process of urbanization has become one of the urgent problems facing policy makers. This paper studies the coupling coordination relationship between new-type urbanization and water ecological environment, with the purpose of using insights gained from the study to help improve the quality of water ecological environment and promote sustainable development of water ecological environment. We take 11 cities in China's Jiangxi Province as the research object, and construct the coupling coordination evaluation indicator system of new-type urbanization and water ecological environment, then using the coupling coordination degree model to examine the state of coupling coordination between new-type urbanization and water ecological environment from 2009 to 2019. We further explore its driving factors employing random effect panel Tobit model. The results show that: (1) The level of new-type urbanization in Jiangxi Province shows a steady upward trend, and the water ecological environment level tends to rise steadily and slowly, although the comprehensive score of water ecological environment in most cities is lower than 0.1, indicating that the situation of water ecological environment is not optimal yet and there is room for improvement. (2) In 2009, 2014 and 2019, the coupling coordination development level between new-type urbanization and water ecological environment in Jiangxi Province showed an upward trend, from moderate maladjustment recession to mild maladjustment recession, and from low coupling coordination to moderate coupling coordination, although the overall coupling coordination degree was low. (3) The investment in scientific and technological innovation, degree of opening-up and government capacity are positively correlated with the coupling coordination degree, while economic development level, resource agglomeration ability, education level and industrialization level are negatively correlated with the coupling coordination degree. These results can provide insights to support new-type urbanization and water ecological environment in the future, and hold great significance for urban sustainable development.