RESUMO
A novel skeleton alkaloid named Chinese Bittersweet Alkaloid III from the leaves of C. angulatus was reported. The structure and its stereochemistry were established by IR, ID and 2DNMR (1H-1HCOSY, HMQC, HMBC, NOESY), MS and elemental analysis.
Assuntos
Alcaloides/isolamento & purificação , Celastraceae/química , Medicamentos de Ervas Chinesas/isolamento & purificação , Piperazinas/isolamento & purificação , Piperidinas/isolamento & purificação , Alcaloides/química , Medicamentos de Ervas Chinesas/química , Espectrometria de Massas , Conformação Molecular , Estrutura Molecular , Ressonância Magnética Nuclear Biomolecular , Piperazinas/química , Piperidinas/química , Folhas de Planta/química , Plantas Medicinais/química , Sementes/química , Espectrofotometria Infravermelho , Espectrofotometria Ultravioleta , EstereoisomerismoRESUMO
AIM: To study the role of interleukin-2 (IL-2) and mediobasal hypothalamus (MBH) in analgesia produced by Coriolus versicolor polysaccharide peptide (PSP). METHODS: The IL-2 antiserum was injected i.c.v. or i.p. and the MBH was destroyed electrolytically. RESULTS: PSP i.g. 1 g.kg-1.d-1 for 6 d increased the pain threshold in tail stimulation-vocalization test in rats. This PSP-produced analgesia was blocked by i.c.v., but not i.p., IL-2 antiserum and disappeared after electrolytic lesion of MBH. CONCLUSION: The analgesia produced by PSP is mediated by IL-2 which is activated by PSP and interacts with IL-2 receptors in the MBH.
Assuntos
Analgésicos não Narcóticos/farmacologia , Hipotálamo Médio/fisiologia , Polyporaceae , Polissacarídeos/farmacologia , Animais , Feminino , Soros Imunes/farmacologia , Injeções Intraventriculares , Interleucina-2/imunologia , Interleucina-2/fisiologia , Masculino , Limiar da Dor , Peptídeos/isolamento & purificação , Peptídeos/farmacologia , Polyporaceae/química , Polissacarídeos/isolamento & purificação , Ratos , Ratos WistarRESUMO
The effect of microinjection of corticotropin releasing hormone (CRH) into the central nucleus of amygdala (CeA) on blood pressure was examined in Wistar rats anaesthetized with pentobarbital sodium. The results were as follows: (1) Microinjection of CRH 100, 500 ng into the CeA produced a dose-dependent increase in blood pressure. The response occurred 5 min after injection and lasted for at least 1 h. (2) The response could be blocked by injection of CRH receptor antagonist alpha-helical CRH9-41 into CeA. (3) Lateral ventricle injection of naloxone could also attenuate the pressor response of CRH. (4) After injection of alpha-helical CRH9-41 into nucleus of solitary tract, the pressor response of CRH was diminished. The results suggest that the pressor effect induced by microinjection of CRH into the CeA might be partly mediated through opioid system via a descending pathway from CeA to NTS.
Assuntos
Tonsila do Cerebelo/fisiologia , Pressão Sanguínea/efeitos dos fármacos , Hormônio Liberador da Corticotropina/farmacologia , Animais , Masculino , Microinjeções , Naloxona/farmacologia , Ratos , Ratos Wistar , Núcleo Solitário/fisiologiaRESUMO
It has been demonstrated in animal model of somatic pain that hypothalamic paraventricular nucleus (PVN) participates in acupuncture analgesia, probably by mediation of vasopressin release. The role of PVN in acupuncture analgesia for experimental visceral pain in rats was further investigated in the present study. Experimental results demonstrated that electroacupuncture could inhibit the writhing response, produced by intraperitoneal injection of antimonium potassium tartrate and this inhibitory effect could be enhanced by electrical stimulation of PVN, but decreased by electrolytical lesion of PVN, intracerebroventricular injection of vasopressin antiserum (14 microliters) or the vasopressin antagonist, d(CH2)5Tyr(Me)-AVP (500 ng/5 microliters). Intraperitoneal administration of the latter drug (10 micrograms/kg), however, was ineffective. The above experimental results suggest that vasopressinergic neurons in PVN also participate in the inhibition of visceral pain by electroacupuncture.
Assuntos
Eletroacupuntura , Nociceptores/fisiologia , Núcleo Hipotalâmico Paraventricular/fisiologia , Receptores de Vasopressinas/fisiologia , Analgesia por Acupuntura , Animais , Arginina Vasopressina/análogos & derivados , Arginina Vasopressina/antagonistas & inibidores , Arginina Vasopressina/imunologia , Feminino , Soros Imunes , Masculino , Limiar da Dor , Ratos , Ratos Wistar , Vasopressinas/antagonistas & inibidores , Vasopressinas/imunologiaRESUMO
Previous studies have demonstrated that locus coeruleus (LC)-noradrenergic neuronal system plays an important role in pain modulation and electroacupuncture (EA) analgesia. In the present experiment, the effect of LC stimulation and EA on nociceptive response of spinal dorsal horn neurons was investigated. The main results were: 1) LC stimulation and electroacupuncture produced a significant inhibitory effect on nociceptive response of dorsal horn neurons; 2) The inhibitory effect of LC stimulation was not affected by the lesion of nucleus raphe magnus or by the injection of naloxone; 3) These inhibitory effects of LC stimulation and electroacupuncture could be enhanced by alpha 2-agonist clonidine, and decreased slightly by alpha-antagonist phentolamine. These results suggest that the inhibitory effect of LC stimulation and electroacupuncture on the nociceptive response of dorsal horn neurons might be mediated by alpha 2-receptors.