Landscape pattern prediction method based on ANN-CA-Markov coupling model.

The simulation and prediction of landscape patterns can provide a reference for the formulation of ecological space security policies and management and control measures. Therefore, it is necessary to use objective and accurate scientific methods to make accurate simulation predictions. In this study, the quantitative prediction of the Markov model is combined with the advantages of the space-time simulation of the CA model, and the artificial neural network (ANN) module is used for calculation and coupling correction. The Kappa coefficient and the FoM (figure of merit) index are used to test the simulation accuracy. Based on this process, the changes of the landscape pattern in Mudanjiang City at three time points in 2000, 2010 and 2020 were analyzed, and the simulation prediction was made based on this. The experimental results show that the CA-Markov model using the ANN module for coupling correction can be tested for accuracy in the study area, and its Kappa coefficient is 0.834 and the FoM index is 0.001. The model meets the requirements of the landscape pattern for the accuracy of simulation and prediction, and the prediction and analysis of the study area is carried out, and the accurate prediction results are obtained. The results of the study were: Looking at the change in landscape pattern over the 20 years from 2000 to 2020, significant changes occurred in the areas of arable land and artificial surface. The total area of arable land decreased by nearly 130 km2, while the area of artificial surface (i.e., built-up land) increased by about 167 km2. The forest area initially decreased and then increased, with an overall increase of approximately 56 km2, while the water and grassland areas first increased and then decreased. In terms of spatial distribution, the changes in agricultural and forest land are mainly concentrated in the central area where urban development is intensive and in the northern forest area. The results of this study are quite important for policy formulation, according to which it was found that human activities, especially urbanization, have led to a decrease in the area of forested land, and therefore the implementation of the natural forest protection policy can lead to a sustained increase in the area of forested land.

Nickel molybdate/cobalt iron carbonate hydroxide heterojunction with oxygen vacancy enables interfacial synergism to trigger oxygen evolution reaction.

The development of electrocatalysts with excellent performance toward oxygen evolution reaction (OER) for the production of hydrogen is of great significance to alleviate energy crisis and environmental pollution. Herein, the heterostructure (NMO/FCHC-0.4) was fabricated by the coupling growth of NiMoO4 (NMO) and cobalt iron carbonate hydroxide (FCHC) on nickel foam as an electrocatalyst for OER. The interfacial synergy on NMO/FCHC-0.4 heterojunction can promote the interfacial electron redistribution, affect the center position of d band, optimize the adsorption of intermediate, and improve the conductivity. Beyond, oxygen defect sites are conducive to the adsorption of intermediates, and increase the number of active sites. Real-time OER kinetic simulation revealed that the interfacial synergism and molybdate could reduce the adsorption of hydroxide, promote the deprotonation step of M-OH, and facilitate the formation of M-OOH (M represents the metal active site). As a result, NMO/FCHC-0.4 displays excellent OER electrocatalytic performance with an overpotential of 250/280 mV at the current density 100/200 mA cm-2 and robust stability at 100 mA cm-2 for 100 h. This work provides deep insights into the roles of interfacial electronic modulation and oxygen vacancy to design high-efficiency electrocatalysts for OER.

Electronic modulation and reaction-pathway optimization on three-dimensional seaweed-like NiSe@NiMn LDH heterostructure to trigger effective oxygen evolution reaction.

The development of low-cost and high-efficiency electrocatalysts for the oxygen evolution reaction (OER) is essential to produce high-purity hydrogen in large scale. Herein, a three-dimensional (3D) seaweed-like hierarchical structure was fabricated using two-dimensional (2D) NiMn LDH nanosheets wrapped on one-dimensional (1D) NiSe nanowires with nickel foam (NF) as a substrate (NiSe@NiMn LDH/NF) via hydrothermal and electrodeposition processes. Owing to the strong interfacial synergy, 3D seaweed-like hierarchical structure, higher conductivity, and strong structural stability, the NiSe@NiMn LDH/NF exhibited superior OER performance with an overpotential of 287 mV at 100 mA cm-2, and stably operated for 160 h at large current. Moreover, the overall water splitting system with NiSe@NiMn LDH/NF as the anode and Pt/C/NF as the cathode exhibited a low cell voltage of 1.59/1.64 V to reach 50/100 mA cm-2, and excellent stability for 110 h at 300 mA cm-2. The density function theory (DFT) calculations unveiled that NiSe@NiMn LDH enabled the interfacial synergy, reallocating the electron density at the interface, and further weakening the energy barrier of OH* by strengthening chemical bonds with OH* intermediates to improve the intrinsic OER activity.

Coupling effect and electronic modulation for synergistically enhanced overall alkaline water splitting on bifunctional Fe-doped CoBi/CoP nanoneedle arrays.

Designing bifunctional electrocatalysts with high efficiency and low cost for water splitting is urgently required for the production of green hydrogen. Herein, a bifunctional iron-doped cobalt borate/cobalt phosphide hybrid supported on nickel foam (Fe-CoBi/CoP/NF) was fabricated via hydrothermal and phosphating process. Benefit from the unique nanoneedle architecture for faster mass transfer, the existence of borate on CoBi for accelerating proton transfer, the moderate adsorption of H* species on CoP, Fe doping and the synergistic effect between CoBi and CoP, Fe-CoBi/CoP/NF hybrid exhibits a low overpotential of 137 mV and 260 mV at 100 mA cm-2 for hydrogen evolution reaction (HER) and oxygen evolution reaction (OER), respectively. Moreover, Fe-CoBi/CoP/NF||Fe-CoBi/CoP/NF also presents a low cell potential of 1.65 V@100 mA cm-2 for overall alkaline water splitting and excellent durability (128 h) without decay. This work provides a new insight into the design of bifunctional electrocatalysts simultaneously through the morphological engineering and heteroatomic doping.

La and S Co-Doping Induced the Synergism of Multiphase Nickel-Iron Nanosheets with Rich Oxygen Vacancies to Trigger Large-Current-Density Oxygen Evolution and Urea Oxidation Reactions.

The development of cost-effective electrocatalysts for oxygen evolution reaction (OER) and urea oxidation reaction (UOR) is of great significance for hydrogen production. Herein, La and S co-doped multiphase electrocatalyst (LSFN-63) is fabricated by metal-corrosion process. FeOOH can reduce the formation energy of NiOOH, and enhance the stability of NiOOH as active sites for OER/UOR. The rich oxygen vacancies can increase the number of active sites, optimize the adsorption of intermediates, and improve electrical conductivity. Beyond, La and S co-doping can also regulate the electronic structure of FeOOH. As a result, LSFN-63 presents a low overpotential of 210/450 mV at 100/1000 mA cm-2 , small Tafel slope (32 mV dec-1 ), and outstanding stability under 1000 mA cm-2 @60 h, and can also display excellent OER activity with 180 mV at 250 mA cm-2 and long-term catalytic durability at 250 mA cm-2 @135 h in 30 wt% KOH under 60 °C. Moreover, LSFN-63 demonstrates remarkable UOR performance in 1 m KOH + 0.5 m urea, which just requires an ultra-small overpotential of 140 mV at 100 mA cm-2 , and maintain long-term durability over 120 h. This work opens up a promising avenue for the development of high-efficiency electrocatalysts by a facile metal-corrosion strategy.

The Establishment of a Tobramycin-Responsive Whole-Cell Micro-Biosensor Based on an Artificial Ribozyme Switch.

In this study, a tobramycin concentration-dependent whole-cell micro-biosensor (tob-HHAz) was constructed by fusing a tobramycin aptamer with a hammerhead ribozyme (HHR) from Schistosoma mansoni. The biosensor was obtained by integrating all the modules into one complete RNA sequence, which was easily introduced into E. coli without suffering from harsh external environments. Three independent tobramycin-sensitive RNA structures were identified via high-throughput screening in vivo and were further verified in vitro to undergo the desired self-cleavage reaction. The computation prediction of the RNA structure was performed to help analyze the mechanisms of various conformations by performing a qualitative and rapid detection of tobramycin in practical samples; two sensors exhibited high responsiveness to spiked milk, with a detection limit of around 40 nM, which is below the EU's antibiotic maximum residual level. One of the structures provides a linear range from 30 to 650 nM with a minimum detection limit of 30 nM and showed relatively good selectivity in spiked urine. This study is the first in which in vivo screening was combined with computation analysis to optimize the pivotal structure of sensors. This strategy enables researchers to use artificial ribozyme-based biosensors not only for antibiotic detection but also as a generally applicable method for the further detection of substances in living cells.

Genome-wide analysis of dysregulated RNA-binding proteins and alternative splicing genes in keloid.

Introduction: The pathogenesis of keloids remains unclear. Methods: In this study, we analyzed RNA-Seq data (GSE113619) of the local skin tissue of 8 keloid-prone individuals (KPI) and 6 healthy controls (HC) before and 42 days after trauma from the gene expression omnibus (GEO) database. The differential alternative splicing (AS) events associated with trauma healing between KPIs and HCs were identifified, and their functional differences were analyzed by gene ontology (GO) and kyoto encyclopedia of genes and genomes (KEGG) pathways. The co-expression relationship of differentially alternative splicing genes and differentially expressed RNA binding proteins (RBPs) was established subsequently. Results: A total of 674 differential AS events between the KD42 and the KD0 and 378 differential AS events between the HD42 and the HD0 were discovered. Notably, most of the differential genes related to keloids are enriched in actin, microtubule cells, and cortical actin cytoskeletal tissue pathway. We observed a signifificant association between AS genes (EPB41, TPM1, NF2, PARD3) and trauma healing in KPIs and HCs. We also found that the differential expression of healthy controls-specifific trauma healing-related RBPs (TKT, FDPS, SAMHD1) may affect the response of HCs to trauma healing by regulating the AS of downstream trauma healing-related genes such as DCN and DST. In contrast, KPIs also has specifific differential expression of trauma healing related RBPs (S100A9, HspB1, LIMA1, FBL), which may affect the healing response of KPIs to trauma by regulating the AS of downstream trauma healing-related genes such as FN1 and TPM1. Discussion: Our results were innovative in revealing early wound healing-related genes (EPB41, TPM1, NF2, PARD3) in KPI from the perspective of AS regulated by RBPs.

Self-sacrificial reconstruction of MoO42- intercalated NiFe LDH/Co2P heterostructures enabling interfacial synergies and oxygen vacancies for triggering oxygen evolution reaction.

Exploring high-efficiency electrocatalysts for oxygen evolution reaction (OER) is one of the most important concerns to produce hydrogen in water electrolysis. Herein, the FNM/Co2P-0.4 heterostructure was designed as an electrocatalyst for the OER process by the combination of MoO42- intercalating NiFe LDH and Co2P on nickel foam (NF). The surface reconstruction and MoO42- leaching can induce the conversion of Co2P and NiFe LDH on FNM/Co2P-0.4 to generate Co/NiOOH with more oxygen vacancies. Beyond, CoOOH and NiOOH can also synergize to reduce the energy barrier of OER, optimize conductivity, and improve stability. The surface reconstruction and the formation of OOH⁎ were further unveiled by in-situ UV-vis absorption spectra and Fourier-transformed alternative current voltammetry (FTACV). The integration of interfacial synergies and oxygen vacancies can facilitate the adsorption/desorption of intermediates, regulate the d-band center, and expose more active sites. And as a result, FNM/Co2P-0.4 shows a significant low overpotential (240 mV) at 50 mA cm-2, a small Tafel (74 mV dec-1), low activation energy (Ea) and remarkable durability. This work provides a new pathway to improve the OER performance by using interfacial synergies and rich oxygen vacancies derived from the self-sacrificial reconstruction of heterostructured electrocatalysts.

Sports knee joint injury in ski training and its prevention / Lesão esportiva da articulação do joelho em treino de esqui e sua prevenção / Lesión de la articulación de la rodilla en el entrenamiento de esquí y su prevención

ABSTRACT Introduction Ski training presents the high ability of athletes and strong demand on the physical conditioning of the knee joint, which is frequently injured. Objective Study the sports injuries of the knee joint in ski training and explore its preventive measures. Methods The interview method was used with ski instructors, enthusiasts, and athletes by questionnaire with design and distribution of topics related to the occurrence of injuries. Sport injury factors are discussed internally and externally. Preventive measures and protective equipment for knee sports injuries are presented. Results Currently, the proportion of serious sports injuries to the knee joint is relatively high, and problems such as collision are the most common injury factors, and differences exist between male and female athletes. Among the causes of injury, the highest-scoring technical factor for injury was "deviation from technical movements", and the highest-scoring preventive measures were "strength training for vulnerable parts" and "sufficient preparation for activities". Conclusion The instructors should provide effective training programs according to the actual situation of the athletes, pay attention to monitoring the skiing environment, and recommend the appropriate protective equipment for the sport. Level of evidence II; Therapeutic studies - investigation of treatment results.

RESUMO Introdução O treino de esqui apresenta alta habilidade dos atletas e forte demanda no condicionamento físico da articulação do joelho, que é frequentemente lesionada. Objetivo Estudar as lesões esportivas da articulação do joelho no treino de esqui e explorar suas medidas preventivas. Métodos O método de entrevista foi usado com instrutores, entusiastas e esportistas de esqui por questionário com projetação e distribuição de tópicos relacionados à ocorrência das lesões. Os fatores de lesão esportiva são discutidos interna e externamente. Medidas preventivas e equipamentos de proteção para lesões esportivas no joelho são apresentados. Resultados Atualmente, a proporção de lesões esportivas graves na articulação do joelho é relativamente alta e problemas como colisão são os fatores de lesão mais comuns, existindo diferenças entre atletas do sexo masculino e feminino. Entre as causas de lesão, o fator técnico de maior pontuação para lesão foi "desvio de movimentos técnicos", e as medidas preventivas de maior pontuação foram "treinamento de força para partes vulneráveis" e "preparação suficiente para as atividades". Conclusão Os treinadores devem fornecer programas de treino eficazes de acordo com a situação real dos atletas, atentarem-se ao monitoramento do ambiente de esqui e indicar os equipamentos de proteção adequados ao esporte. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.

RESUMEN Introducción El entrenamiento de esquí presenta una alta capacidad de los deportistas y una fuerte exigencia en el acondicionamiento físico de la articulación de la rodilla, que se lesiona con frecuencia. Objetivo Estudiar las lesiones deportivas de la articulación de la rodilla en el entrenamiento de esquí y explorar sus medidas preventivas. Métodos Se utilizó el método de la entrevista con instructores de esquí, aficionados y deportistas mediante un cuestionario con diseño y distribución de temas relacionados con la ocurrencia de las lesiones. Los factores de las lesiones deportivas se discuten interna y externamente. Se presentan las medidas preventivas y los equipos de protección para las lesiones deportivas de rodilla. Resultados En la actualidad, la proporción de lesiones deportivas graves en la articulación de la rodilla es relativamente alta y problemas como la colisión son los factores de lesión más comunes, y existen diferencias entre los deportistas masculinos y femeninos. Entre las causas de las lesiones, el factor técnico con mayor puntuación fue la "desviación de los movimientos técnicos", y las medidas preventivas con mayor puntuación fueron el "entrenamiento de fuerza de las partes vulnerables" y la "preparación suficiente para las actividades". Conclusión Los entrenadores deben proporcionar programas de entrenamiento eficaces en función de la situación real de los deportistas, prestar atención al seguimiento del entorno de esquí e indicar el equipo de protección adecuado para el deporte. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.

Analysis of the effect of high intensity intermittent training for young athletes / Análise do efeito do treinamento intermitente de alta intensidade para jovens atletas / Análisis del efecto del entrenamiento intermitente de alta intensidad en atletas jóvenes

ABSTRACT Introduction The CPC Central Committee and the State Council have pointed out that currently, the basic indicators of young students' physical health have completely declined. This circumstance has made adolescent physical health and improving the quality of young athletes a key issue in physical education targeting adolescents. Objective Investigate the effect of high-intensity intermittent training on young athletes. Methods 24 adolescents performed 10 intermittent high-intensity training for one month, and all athletes' pre and post-training indexes were collected and compared. Results The physical examination results of male and female athletes improved to some extent (athletes' blood pressure fluctuated during the 10 training sessions, increasing slightly, and heart rate variability increased considerably (P < 0.05). Conclusion High-intensity intermittent training has a good effect on health promotion in young athletes, and can effectively improve their physical function and competitive performance. Physical education teachers and student-athletes should conduct relevant training. In addition, this training method also fits the current learning life situation of high school students. Level of evidence II; Therapeutic studies - investigation of treatment results.

RESUMO Introdução O Comitê Central do CPC e o Conselho de Estado destacaram que, atualmente, os indicadores básicos da saúde física dos jovens estudantes têm diminuído de forma completa. Essa circunstância tornou a saúde física dos adolescentes e a melhoria da qualidade dos jovens atletas um ponto-chave na educação física direcionada aos adolescentes. Objetivo Investigar o efeito do treinamento intermitente de alta intensidade em jovens atletas. Métodos 24 adolescentes realizaram 10 treinos intermitentes de alta intensidade, com duração de um mês, todos os índices pré e pós-treino dos atletas foram coletados e comparados. Resultados Os resultados do exame físico de atletas do sexo masculino e feminino melhoraram em certa medida (a pressão arterial dos atletas flutuou durante as 10 sessões de treinamento, aumentando ligeiramente, e a variabilidade da frequência cardíaca aumentou consideravelmente (P < 0,05). Conclusão O treinamento intermitente de alta intensidade tem um bom efeito na promoção da saúde nos jovens atletas, podendo melhorar efetivamente sua função física e desempenho competitivo. Professores de educação física e atletas estudantes devem realizar treinamentos relevantes. Além disso, esse método de treinamento também se encaixa na situação atual da vida de aprendizagem dos alunos do ensino médio. Nível de evidência II; Estudos terapêuticos - investigação dos desfechos do tratamento.

RESUMEN Introducción El Comité Central del CPC y el Consejo de Estado han puesto de relieve que en la actualidad los indicadores básicos de salud física de los jóvenes estudiantes han disminuido completamente. Esta circunstancia hizo que la salud física de los adolescentes y la mejora de la calidad de los jóvenes deportistas fuera un punto clave en la educación física dirigida a los adolescentes. Objetivo Investigar el efecto del entrenamiento intermitente de alta intensidad en atletas jóvenes. Métodos 24 adolescentes realizaron 10 sesiones de entrenamiento intermitente de alta intensidad de un mes de duración, se recogieron y compararon los índices de todos los atletas antes y después del entrenamiento. Resultados Los resultados de la exploración física de los atletas masculinos y femeninos mejoraron en cierta medida (la presión arterial de los atletas fluctuó durante las 10 sesiones de entrenamiento, aumentando ligeramente, y la variabilidad de la frecuencia cardíaca aumentó considerablemente (P < 0,05). Conclusión El entrenamiento intermitente de alta intensidad tiene un buen efecto en la promoción de la salud en los atletas jóvenes, y puede mejorar eficazmente su función física y su rendimiento competitivo. Los profesores de educación física y los alumnos deportistas deben realizar la formación pertinente. Además, este método de formación también se ajusta a la situación actual de la vida de aprendizaje de los estudiantes de secundaria. Nivel de evidencia II; Estudios terapêuticos - investigación de los resultados del tratamiento.

Fatigue and rehabilitation in the training of gymnasts / Fadiga e reabilitação no treinamento de ginastas / Fatiga y rehabilitación en el entrenamiento de gimnastas

ABSTRACT Introduction Aerobic gymnastics requires a lot of physical ability and endurance. Fatigue is an inevitable consequence of intrinsic movements with a high intensity related to the sport. Objective Analyze the rehabilitation strategy of sports fatigue in aerobic gymnastics athletes caused by training. Methods 20 volunteer aerobics students in colleges and universities were recruited and divided into experimental and control groups. High-intensity aerobic gymnastics training was performed where the experimental group used a combined exercise fatigue recovery scheme, while the control group used only traditional walking and stretching. Results Lasting 40 minutes, the post-exercise fatigue rehabilitation protocol showed a decreasing trend in muscle stress, while the control group evidenced a fluctuating decreasing trend. The recovery frequency of the experimental group was higher than that of the control group. Conclusion The combined method of rehabilitation training mentioned in this paper can better regulate the heart rate of athletes, reduce the level of fatigue, and transform passive relaxation into active sports rehabilitation, engaging the enthusiasm of sport in athletes. Therefore, the scheme proposed in this paper has better practical significance and practical value. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.

RESUMO Introdução A ginástica aeróbica requer muita habilidade física e resistência. A fadiga é uma consequência inevitável dos movimentos intrínsecos com alta intensidade relacionados ao esporte. Objetivo Analisar a estratégia de reabilitação da fadiga esportiva nos atletas de ginástica aeróbica ocasionada pelo treinamento. Métodos 20 voluntários estudantes de aeróbica em faculdades e universidades foram recrutados e divididos em grupo experimental e controle. Foi realizado um treinamento de ginástica aeróbica de alta intensidade onde o grupo experimental utilizou um esquema de recuperação de fadiga de exercício combinado, enquanto o grupo de controle utilizou apenas a caminhada e o alongamento tradicionais. Resultados Com duração de 40 minutos, o protocolo de reabilitação de fadiga após o exercício mostrou uma tendência decrescente do estresse muscular, enquanto o grupo controle evidenciou uma tendência decrescente flutuante. A frequência de recuperação do grupo experimental foi maior do que a do grupo controle. Conclusão a utilização do método combinado de treinamento de reabilitação mencionado neste trabalho pode regular melhor a frequência cardíaca dos atletas, reduzir o nível de fadiga e transformar o relaxamento passivo em reabilitação esportiva ativa, engajando um entusiasmo do esporte nos atletas. Portanto, o esquema proposto neste trabalho tem melhor significado prático e valor prático. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.

RESUMEN Introducción La gimnasia aeróbica requiere mucha capacidad física y resistencia. La fatiga es una consecuencia inevitable de los movimientos intrínsecos de alta intensidad relacionados con el deporte. Objetivo Analizar la estrategia de rehabilitación de la fatiga deportiva en atletas de gimnasia aeróbica causada por el entrenamiento. Métodos Se reclutaron 20 voluntarios estudiantes de aeróbic en colegios y universidades y se dividieron en grupo experimental y de control. Se realizó un entrenamiento de gimnasia aeróbica de alta intensidad en el que el grupo experimental utilizó un esquema combinado de recuperación de la fatiga del ejercicio, mientras que el grupo de control sólo utilizó la caminata y los estiramientos tradicionales. Resultados Con una duración de 40 minutos, el protocolo de rehabilitación tras la fatiga mostró una tendencia a la disminución del estrés muscular, mientras que el grupo de control evidenció una tendencia a la disminución fluctuante. La frecuencia de recuperación del grupo experimental fue mayor que la del grupo de control. Conclusión El uso del método combinado de entrenamiento de rehabilitación mencionado en este artículo puede regular mejor el ritmo cardíaco de los atletas, reducir el nivel de fatiga y transformar la relajación pasiva en una rehabilitación deportiva activa, despertando el entusiasmo por el deporte en los atletas. Por lo tanto, el esquema propuesto en este trabajo tiene mayor importancia práctica y valor práctico. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.

Doping Mo into NiFe LDH/NiSe Heterostructure to Enhance Oxygen Evolution Activity by Synergistically Facilitating Electronic Modulation and Surface Reconstruction.

It is of great significance to design highly efficient electrocatalysts with abundant earth elements instead of precious metals for water splitting. Herein, Mo-doped NiFe-layered double hydroxides/NiSe heterostructure (Mo-NiFe LDH/NiSe) was fabricated by coupling Mo-doped NiFe LDH and NiSe on nickel foam (NF). The heterostructure electrocatalyst showed ultra-low overpotential (250 mV) and remarkable durability for oxygen evolution reaction (OER) at 150 mA cm-2 . Both theoretical and experimental results confirmed that Mo doping and interfacial synergism induced the interfacial charge redistribution and the lifted d-band center to weaken the energy barrier (EB) of the formation of OOH* . Mo doping also facilitated the surface reconstruction of NiFe LDH into Ni(Fe)OOH as the active sites under electro-oxidation process. This work provides a facile strategy for electronic modulation and surface reconstruction of OER electrocatalyst by transition metal doping and heterostructure generation.

Synergistic coupling of heterostructured porous CoP nanosheets with P doped NiO for highly efficient overall alkaline water splitting.

Exploring non-noble metal materials as bifunctional catalysts for water electrolysis is of great significance for the development and utilization of hydrogen energy. Herein, a flower branch-leaf shaped phosphide/oxide heterogeneous electrocatalyst located on Ni foam (CoP/P-NiO/NF) was developed through hydrothermal and phosphorization strategy. Benefiting from the strong ability to dissociate H2O molecules on P-NiO and the suitable adsorption of intermediate H species on CoP, the optimal CoP/P-NiO/NF exhibited outstanding performance with low overpotentials of 52 mV at current density of 10 mA cm-2, smaller Tafel slopes of 73.6 mV dec-1 for hydrogen evolution reaction (HER). Meanwhile, CoP/P-NiO/NF indicated 265 mV at 100 mA cm-2 with Tafel slope of 101.8 mV dec-1 for oxygen evolution reaction (OER) due to the optimal redistribution of electrons among Ni2+, Co2+ and Co3+ for favorable adsorption/desorption of oxygen-intermediates. Both HER and OER shown robust stability during 32 h without decline. The corresponding two-electrode system for overall alkaline water splitting required a low voltage of 1.6 V at 100 mA cm-2 with long stability (20 h) which is far lower than that on RuO2-Pt/C and many other reported non-noble metal electrocatalysts. This work demonstrates that the synergistic effect and morphology engineering play vital roles in the enhanced electrocatalytic performance.

A Maluku Sea intermediate western boundary current connecting Pacific Ocean circulation to the Indonesian Throughflow.

The Indonesian Throughflow plays an important role in the global ocean circulation and climate. Existing studies of the Indonesian Throughflow have focused on the Makassar Strait and the exit straits, where the upper thermocline currents carry North Pacific waters to the Indian Ocean. Here we show, using mooring observations, that a previous unknown intermediate western boundary current (with the core at ~1000 m depth) exists in the Maluku Sea, which transports intermediate waters (primarily the Antarctic Intermediate Water) from the Pacific into the Seram-Banda Seas through the Lifamatola Passage above the bottom overflow. Our results suggest the importance of the western boundary current in global ocean intermediate circulation and overturn. We anticipate that our study is the beginning of more extensive investigations of the intermediate circulation of the Indo-Pacific ocean in global overturn, which shall improve our understanding of ocean heat and CO2 storages significantly.

Electronic modulation and proton transfer by iron and borate co-doping for synergistically triggering the oxygen evolution reaction on a hollow NiO bipyramidal prism.

Designing an Earth-abundant and inexpensive electrocatalyst to drive the oxygen evolution reaction (OER) for high-purity hydrogen production is of great importance. Herein, the cation (iron) and anion (borate) co-doping strategy was proposed to effectively trigger the OER performance on a low-cost NiO material. The optimal hollow Fe/Bi-NiO bipyramidal prism shows superior OER performance, and displays a low overpotential (261 mV) at 10 mA cm-2, accompanied by a low Tafel slope (46 mV dec-1), excellent intrinsic activity and robust stability. The overall alkaline water splitting using Fe/Bi-NiO/NF as an anode affords low cell voltages of 1.50 and 1.63 V at 10 and 100 mA cm-2, and operates steadily at a high current density of 100 mA cm-2 for 55 h without decay. The excellent electrocatalytic activity could be ascribed to the hollow structure to shorten the mass transfer pathway, the electronic modulation by Fe doping, the increased accessible electroactive sites created by oxygen vacancies through borate doping, and the formation of BO33--OH- to accelerate the deprotonation of OHads.

Hierarchical sheet-on-sheet heterojunction array of a ß-Ni(OH)2/Fe(OH)3 self-supporting anode for effective overall alkaline water splitting.

Rationally designing high-performance non-noble metal electrocatalysts is of essence to improve energy conversion efficiency in water splitting. Herein, a unique 3D hierarchical sheet-on-sheet heterojunction between Fe(OH)3 and ß-Ni(OH)2 on pretreated Ni foam (NiFe-HD/pre-NF) was fabricated by a two-step strategy involving the interfacial hydrolysis-deposition of Fe2+ and electrodeposition of Ni2+. The presence of the Ni-O-Fe bridge at the Fe(OH)3/ß-Ni(OH)2 heterointerface can induce interfacial electronic redistribution to form Ni3+ in NiFe-HD/pre-NF, and further strengthen the adsorption of OH- and weaken the O-H bond to change the rate-determining step (RDS) for accelerating OER kinetics. Benefiting from the sheet-on-sheet architecture and dual-phase synergism on NiFe-HD/pre-NF, the optimal NiFe-HD/pre-NF exhibits excellent OER performance with a lower overpotential of 256 mV at 100 mA cm-2, a small Tafel slope of 81 mV dec-1, high intrinsic activity and robust stability. Alkaline water-splitting using NiFe-HD/pre-NF as the anode requires ultralow cell voltages of 1.62 V and 1.83 V at current densities of 100 mA cm-2 and 400 mA cm-2, respectively, which are comparable with commercial alkaline water electrolysis, and operates steadily at a current density of 100 mA cm-2 for 85 h without decay. This work proposes a facile strategy for constructing heterojunctions and modulating electronic interaction to develop electrocatalysts with new architectures.

Molecular profiling of TOX-deficient neoplastic cells in cutaneous T cell lymphoma.

Cutaneous T cell lymphoma (CTCL) is a rare but potentially devastating primary cutaneous lymphoma. CTCL is characterized by localization of neoplastic T lymphocytes to the skin, with mycosis fungoides (MF) and its leukemic form, Sézary syndrome (SS) being the most common variants. Thymocyte selection-associated high-mobility group box (TOX) gene has been found to be highly expressed in MF and SS. It is reported that higher expression levels of TOX in patients will increase risks of disease progression and poor prognosis. However, the molecular events leading to these abnormalities have not been well understood. To better understand the molecular mechanism underlying TOX-mediated differentially expressed genes (DEGs) in CTCL, and to identify DEGs pathways triggered after knockdown of TOX gene in the CTCL cell line Hut78, we employed two shRNA-mediated lentiviruses to knock down TOX gene in the skin lymphoma cell line HuT78. RNA sequencing (RNAseq) analysis was applied to analyze DEGs, DEGs GO and their corresponding pathways. Knockdown of TOX can induce upregulation of 547 genes and downregulation of 649 genes, respectively. HOXC9 was the most significant downregulated gene. Most DEGs are enriched in malignancies and relate to the Wnt and mTOR signaling pathways, and therefore they can regulate cellular processes and induce different biological regulation. Transcriptome analysis of DEGs after knockdown of TOX in our study provides insights into the mechanism of TOX in CTCL and suggests candidate targets for therapy of CTCL.

IL28RA inhibits human epidermal keratinocyte proliferation by inhibiting cell cycle progression.

Interleukin (IL) 28 receptor α (IL28RA) is a well-known candidate for psoriasis susceptibility based on previous genome-wide association study (GWAS) analysis. However, the function of IL28RA in psoriasis has not been elucidated. In the present study, the expression of IL28RA was significantly decreased in lesional tissues from patients with plaque psoriasis when compared with the expression observed in adjacent non-lesional tissues. In vitro studies further demonstrated that in the presence of IL-29, HaCaT keratinocytes with IL28RA knockdown exhibited a faster rate of proliferation than control cells, and an enhanced ratio of cells in the S and G2/M phase. By contrast, IL28RA overexpression inhibited the proliferation of HaCaT keratinocytes and caused cell cycle arrest at the G0/G1 phases. Western blot analysis revealed that knockdown of IL28RA upregulated cyclinB1 expression and downregulated cyclinE expression; the opposite results were observed in the IL28RA-overexpressing HaCaT cells. Finally, a mechanistic study revealed that IL28RA functions through the activation of the Janus kinase-signal transducer and activator of transcription signaling pathway to exert its anti-proliferative effect. These results suggested that weak expression of IL28RA may contribute to the pathogenesis of psoriasis and that IL28RA may be an effective drug target for the treatment of psoriasis. However, further in vivo studies are required.