RESUMO
Isoflavone, which are mainly found in soybeans, are a secondary metabolite with a variety of physiological functions. In recent years, increasing the isoflavone content of soybeans has received widespread attention. Although ethephon treatment significantly increased isoflavone content in soybean sprouts, it also had a certain inhibitory effect on the growth of sprouts. Melatonin (MT), as a new type of plant hormone, not only alleviated the damage caused by abiotic stress to plants, but also promoted the synthesis of secondary metabolites. In this study, we aimed to elucidate the mechanism of exogenous MT in regulating the growth and development, and the metabolism of isoflavone in soybean sprouts under ethephon treatment. The results indicated that MT alleviated the adverse effects of ethephon treatment on soybean sprouts by increasing the activities of superoxide dismutase, peroxidase, catalase, and the expression of their corresponding genes, as well as decreased the content of malondialdehyde and hydrogen peroxide. In addition, MT further increased the isoflavone content by up-regulating the expression level of isoflavone synthesis genes and increased the activities of phenylalanine ammonia-lyase and cinnamic acid 4-hydroxylase under ethephon treatment. This study provided technical support and reference value for the production of high-quality soybean sprouts to a certain extent.
Assuntos
Antioxidantes , Etilenos , Glycine max , Isoflavonas , Melatonina , Reguladores de Crescimento de Plantas , Glycine max/metabolismo , Glycine max/efeitos dos fármacos , Glycine max/crescimento & desenvolvimento , Glycine max/genética , Melatonina/metabolismo , Isoflavonas/metabolismo , Isoflavonas/biossíntese , Etilenos/metabolismo , Antioxidantes/metabolismo , Reguladores de Crescimento de Plantas/metabolismo , Plântula/metabolismo , Plântula/efeitos dos fármacos , Plântula/crescimento & desenvolvimento , Compostos Organofosforados/farmacologia , Compostos Organofosforados/metabolismoRESUMO
Phenolic acids are secondary metabolites in higher plants, with antioxidant, anticancer, and anti-aging effects on the human body. Therefore, foods rich in phenolic acids are popular. Methyl jasmonate (MeJA) promoted phenolic acids accumulation but also inhibited sprout growth. Melatonin (MT) was a new type of plant hormone that not only alleviated plants' abiotic stress, but also promoted the synthesis of plant-stimulating metabolism. This study aimed to elucidate the mechanism of exogenous MT on the growth and development, and phenolic acids metabolism of barley sprouts under MeJA treatment. The results showed that MT increased the phenolic acids content in sprouts by increasing the activities of phenylalanine ammonia-lyase and cinnamic acid 4-hydroxylase, and up-regulating the gene expression of phenylalanine ammonia-lyase, cinnamic acid 4-hydroxylase, 4-coumarate: coenzyme a ligase, and ferulic acid-5-hydroxylase. MT attenuated the growth inhibition of barley sprouts under MeJA stress by increasing the activities of regulated antioxidant enzymes and the expression of their corresponding genes. Furthermore, MT increased the NO content and induced Ca2+ burst in barley sprouts under MeJA stress. These events were inhibited by DL-4-Chlorophenylalanine. These results suggested that MT ameliorated growth inhibition and promoted the biosynthesis of phenolic acids in barley sprouts under MeJA stress.
RESUMO
α-Phase formamidinium lead iodide (FAPbI3) perovskite solar cells (PSCs) have garnered significant attention, owing to their remarkable efficiency. Methylammonium chloride (MACl), a common additive, is used to control the crystallization of FAPbI3, thereby facilitating the formation of the photoactive α-phase. However, MACl's high volatility raises concerns regarding its stability and potential impact on the stability of the device. In this study, we partially substituted MACl with n-propylammonium chloride (PACl), which has a long alkyl chain, to promote the oriented crystallization of FAPbI3, ultimately forming an δ-phase-free perovskite. The FAPbI3 film containing PACl demonstrates an enhanced photoluminescence intensity and lifetime. Additionally, PACl's presence at grain boundaries acts as a protective layer for the PSCs. Consequently, we achieved a power conversion efficiency (PCE) of 22.4% and exceptional stability. It maintains over 95% of initial PCE for 100 days in an N2 glovebox, over 85% after 100 h of maximum power point tracking, and over 80% after 60 °C thermal aging.
RESUMO
Germination holds the key to nutritional equilibrium in plant grains. In this study, the effect of soybean germination on the processing of soymilk (SM) and glucono-δ-lactone (GDL) induced soymilk gel (SG) was investigated. Germination promoted soybean sprout (SS) growth by activating the energy metabolism system. The energy metabolism was high during the three-day germination and was the most vigorous on the second day of germination. After germination, protein dissolution was improved in SM, and endogenous enzymes produced small molecule proteins. Small molecule proteins were more likely to aggregate to produce SM protein particles. Germination increased the water-holding capacity of SG induced by GDL but weakened the strength. Furthermore, the dynamic fluctuations in isoflavone content were closely monitored throughout the processing of soybean products, including SS, SM, and SG. Although the total amount of isoflavones in SM and SG processed from germinated soybeans decreased, a significant enrichment in the content of aglycone isoflavones was observed. The content of aglycone isoflavones in SG processed from germinated soybeans on the second day of germination was 736.17 ± 28.49 µg/g DW, which was 83.19 % higher than that of the control group. This study demonstrates that germination can enhance the nutritional value of soybean products, providing innovative opportunities for the development of health-promoting soybean-based products.
Assuntos
Géis , Germinação , Glycine max , Isoflavonas , Leite de Soja , Isoflavonas/análise , Isoflavonas/metabolismo , Leite de Soja/química , Leite de Soja/metabolismo , Glycine max/crescimento & desenvolvimento , Glycine max/química , Glycine max/metabolismo , Manipulação de Alimentos/métodos , Valor Nutritivo , Sementes/química , Sementes/crescimento & desenvolvimento , Sementes/metabolismo , Metabolismo Energético , Lactonas/metabolismo , Lactonas/análiseRESUMO
As the world's population and income levels continue to rise, there is a substantial increase in the demand for meat, which poses significant environmental challenges due to large-scale livestock production. This review explores the potential of microalgae as a sustainable protein source for meat analogues. The nutritional composition, functional properties, and environmental advantages of microalgae are analyzed. Additionally, current obstacles to large-scale microalgal food production are addressed, such as strain development, contamination risks, water usage, and downstream processing. The challenges associated with creating meat-like textures and flavors using techniques like extrusion and emulsion formation with microalgae are also examined. Lastly, considerations related to consumer acceptance, marketing, and regulation are summarized. By focusing on improvements in cultivation, structure, sensory attributes, and affordability, microalgae demonstrate promise as a transformative and eco-friendly protein source to enhance the next generation of meat alternatives.
Assuntos
Carne , Microalgas , Microalgas/química , Microalgas/metabolismo , Microalgas/crescimento & desenvolvimento , Animais , Humanos , Carne/análise , Valor Nutritivo , Substitutos da CarneRESUMO
Exogenous abiotic stimulant treatments are a straightforward and effective method for enhancing secondary metabolites in plants. In this study, the response surface optimization method was used to optimize the conditions for enriching flavonoids in short-germinated black soybeans under a slight acid treatment, and the mechanism of flavonoid accumulation during black soybean germination was explored. The results show that the use of a 126.2 mM citric acid-sodium citrate buffer (pH 5.10) as a slight acid treatment resulted in the highest flavonoid content when the black soybeans were germinated for 24 h. Under these conditions, the isoflavonoid (glycitin, daidzein, and genistein) increased significantly, and the flavonoid content reached 2.32 mg/g FW. The microacidified germination treatment significantly increased the activities and relative gene expression levels of key enzymes involved in flavonoid metabolism (4-coumarate-CoA ligase and cinnamic acid 4-hydroxylase, etc.). However, the slight acid treatment inhibited the growth of the black soybeans and caused damage to their cells. This was evidenced by significantly higher levels of malondialdehyde, superoxide anion, and hydrogen peroxide compared to the control group. Furthermore, the antioxidant system in the short-germinated soybeans was activated by the slight acid treatment, leading to a significant increase in the activities and relative gene expression levels of catalase and peroxidase. The results above show that a slight acid treatment was beneficial in inducing the accumulation of flavonoids during the growth of black soybean sprouts. This lays a technical foundation for producing black soybean products that are rich in flavonoids.
RESUMO
The massive production of food waste and plastic pollution necessitates innovative solutions. This study reports the first fabrication of a flexible chitosan (CH) film reinforced with lignosulfonate (LS) derived from pulping byproduct as a sustainable alternative to synthetic food packaging. The CH/LS composite film was prepared by a simple casting method with varying LS contents of 1 % and 2 %. Compared to CH film, the addition of 2 % LS increased the tensile strength by over 4 times and decreased water vapor permeability by 11 %. Moreover, the CH/LS film exhibited excellent UV-shielding properties. This novel use of LS to reinforce CH film presents an eco-friendly active packaging material. When used to package cherry tomatoes for 2 weeks, the CH/LS film effectively maintained fruit freshness and hardness while minimizing weight loss. This work provides new scientific evidence on the optimized preparation and application of CH/LS composite films from renewable resources for food preservation.
RESUMO
BACKGROUND: Phenolic acid exhibits a variety of well-known physiological functions. In this study, optimal germination conditions to ensure total phenolic acid enrichment in barley sprouts induced by salicylic acid treatment and its effects on sprout physiology and activity, as well as the gene expression of key enzymes for phenolic acid biosynthesis, were investigated. RESULTS: When sprouts were treated with 1 mmol L-1 salicylic acid during germination and germinated at 25 °C for 4 days, the phenolic acid content was 1.82 times that of the control, reaching 1221.54 µg g-1 fresh weight. Salicylic acid significantly increased the activity of phenylalanine aminolase and cinnamic acid-4-hydroxylase and the gene expression of phenylalanine aminolase, cinnamic acid-3-hydroxylase, cinnamic acid-4-hydroxylase, 4-coumaric acid-coenzyme A, caffeic acid O-methyltransferase, and ferulate-5-hydroxylase in barley sprouts. However, salicylic acid treatment significantly increased malondialdehyde and H2O2 content, H2O2 and O2 - fluorescence intensity, as well as significantly decreasing sprout length and fresh weight. Salicylic acid treatment markedly increased the activity of peroxidase and catalase and the gene expression of peroxidase, catalase, and ascorbate peroxidase in barley sprouts. CONCLUSION: Salicylic acid treatment during barley germination significantly promoted the enrichment of total phenolic acid by increasing the activities and gene expression levels of enzymes involved in the phenolic acid biosynthesis pathway. Salicylic acid induced the accumulation of reactive oxygen species, inhibited sprout growth, and activated the antioxidant system. This study provides a basis for the future development of functional foods using phenol acid-rich plants as raw materials. © 2024 Society of Chemical Industry.
Assuntos
Germinação , Hordeum , Hidroxibenzoatos , Proteínas de Plantas , Ácido Salicílico , Hordeum/crescimento & desenvolvimento , Hordeum/metabolismo , Hordeum/efeitos dos fármacos , Hordeum/genética , Ácido Salicílico/farmacologia , Ácido Salicílico/metabolismo , Hidroxibenzoatos/metabolismo , Germinação/efeitos dos fármacos , Proteínas de Plantas/metabolismo , Proteínas de Plantas/genética , Regulação da Expressão Gênica de Plantas/efeitos dos fármacos , Plântula/crescimento & desenvolvimento , Plântula/metabolismo , Plântula/efeitos dos fármacos , Sementes/crescimento & desenvolvimento , Sementes/metabolismo , Sementes/efeitos dos fármacos , Sementes/química , Peróxido de Hidrogênio/metabolismo , Catalase/metabolismo , Catalase/genéticaRESUMO
Ultraviolet (UV) A radiation (315-400 nm) is the predominant component of solar UV radiation that reaches the Earth's surface. However, the underlying mechanisms of the positive effects of UV-A on photosynthetic organisms have not yet been elucidated. In this study, we investigated the effects of UV-A radiation on the growth, photosynthetic ability, and metabolome of the edible cyanobacterium Nostoc sphaeroides. Exposures to 5-15 W m-2 (15-46 µmol photons m-2 s-1) UV-A and 4.35 W m-2 (20 µmol photons m-2 s-1) visible light for 16 days significantly increased the growth rate and biomass production of N. sphaeroides cells by 18%-30% and 15%-56%, respectively, compared to the non-UV-A-acclimated cells. Additionally, the UV-A-acclimated cells exhibited a 1.8-fold increase in the cellular nicotinamide adenine dinucleotide phosphate (NADP) pool with an increase in photosynthetic capacity (58%), photosynthetic efficiency (24%), QA re-oxidation, photosystem I abundance, and cyclic electron flow (87%), which further led to an increase in light-induced NADPH generation (31%) and ATP content (83%). Moreover, the UV-A-acclimated cells showed a 2.3-fold increase in ribulose-1,5-bisphosphate carboxylase/oxygenase activity, indicating an increase in their carbon-fixing capacity. Gas chromatography-mass spectrometry-based metabolomics further revealed that UV-A radiation upregulated the energy-storing carbon metabolism, as evidenced by the enhanced accumulation of sugars, fatty acids, and citrate in the UV-A-acclimated cells. Therefore, our results demonstrate that UV-A radiation enhances energy flow and carbon assimilation in the cyanobacterium N. sphaeroides.IMPORTANCEUltraviolet (UV) radiation exerts harmful effects on photo-autotrophs; however, several studies demonstrated the positive effects of UV radiation, especially UV-A radiation (315-400 nm), on primary productivity. Therefore, understanding the underlying mechanisms associated with the promotive effects of UV-A radiation on primary productivity can facilitate the application of UV-A for CO2 sequestration and lead to the advancement of photobiological sciences. In this study, we used the cyanobacterium Nostoc sphaeroides, which has an over 1,700-year history of human use as food and medicine, to explore its photosynthetic acclimation response to UV-A radiation. As per our knowledge, this is the first study to demonstrate that UV-A radiation increases the biomass yield of N. sphaeroides by enhancing energy flow and carbon assimilation. Our findings provide novel insights into UV-A-mediated photosynthetic acclimation and provide a scientific basis for the application of UV-A radiation for optimizing light absorption capacity and enhancing CO2 sequestration in the frame of a future CO2 neutral, circular, and sustainable bioeconomy.
Assuntos
Nostoc , Raios Ultravioleta , Humanos , Biomassa , Carbono/metabolismo , Dióxido de Carbono/metabolismo , Nostoc/metabolismo , Fotossíntese/fisiologiaRESUMO
The present study investigated the effects regulating melatonin (MT) biosynthesis under methyl jasmonate (MeJA) treatment in mustard sprouts. The results revealed that MeJA significantly increased the MT content in the sprouts to 11.43 times that of the control. However, MeJA treatment had an inhibitory effect on growth. Tryptophan decarboxylase and tryptamine 5-hydroxylase gene expression were significantly induced by MeJA. Moreover, 156 differential abundance proteins (DAPs) were detected in 4-day-old sprouts using quantitative proteomic methods. These DAPs were divided into 13 functional groups, and the vast majority of DAPs involved in defense/stress, energy, signal transduction, and secondary metabolism increased. MeJA treatment significantly enriched 15 pathways, including glutathione metabolism, biosynthesis of secondary metabolites, and tryptophan metabolism. In particular, the abundance of three DAPs (myrosinase 1, cytosolic sulfotransferase 16, and glutamate-glyoxylate aminotransferase 2) in the tryptophan metabolism pathway, a substrate for MT biosynthesis, increased significantly. In summary, MeJA induces endogenous MT biosynthesis in mustard sprouts by promoting the genes expression of MT synthetase and increasing the abundance of tryptophan-related proteins.
RESUMO
Promoting resveratrol accumulation in plants and utilizing resveratrol-rich plants as raw materials for the development of functional foods is a promising development direction. The effects of methyl jasmonate (MeJA), in combination with CaCl2 and Ca2+ inhibitors, on physiological metabolism and resveratrol enrichment of peanut sprouts were investigated. MeJA combined with CaCl2 increased Ca2+ content, calmodulin content, and Ca2+- adenosine triphosphatase activity, as well as upregulated calcium-binding proteinase expression levels. Treatment with MeJA plus CaCl2 significantly increased peroxidase and superoxide dismutase activities and antioxidant capacities, significantly decreased the content of malondialdehyde and hydrogen peroxide, which resulted in a significantly increased in sprout length and fresh weight, and alleviated the inhibition of sprout growth. MeJA plus CaCl2 significantly increased the activities of phenylalanine ammonia-lyase and 4-coumarate coenzyme A ligase and upregulated the expression levels of phenylalanine ammonia-lyase, cinnamic acid 4-hydroxylase, and resveratrol synthase, thus significantly increasing resveratrol content. However, MeJA combined with Ca2+ antagonists reversed these effects. These results indicate that MeJA interacts with Ca2+ to promote resveratrol synthesis in peanut sprouts and to improve sprout stress tolerances.
RESUMO
Microalgae have been recognized as emerging cell factories due to the high value-added bio-products. However, the balance between algal growth and the accumulation of metabolites is always the main contradiction in algal biomass production. Hence, the security and effectiveness of regulating microalgal growth and metabolism simultaneously have drawn substantial attention. Since the correspondence between microalgal growth and reactive oxygen species (ROS) level has been confirmed, improving its growth under oxidative stress and promoting biomass accumulation under non-oxidative stress by exogenous mitigators is feasible. This paper first introduced ROS generation in microalgae and described the effects of different abiotic stresses on the physiological and biochemical status of microalgae from these aspects associated with growth, cell morphology and structure, and antioxidant system. Secondly, the role of exogenous mitigators with different mechanisms in alleviating abiotic stress was concluded. Finally, the possibility of exogenous antioxidants regulating microalgal growth and improving the accumulation of specific products under non-stress conditions was discussed.
Assuntos
Microalgas , Espécies Reativas de Oxigênio/metabolismo , Microalgas/metabolismo , Biomassa , Estresse Oxidativo , Estresse Fisiológico , Antioxidantes/metabolismo , BiocombustíveisRESUMO
Biomanufacturing of ethylene is particularly important for modern society. Cyanobacterial cells are able to photosynthesize various valuable chemicals. A promising platform for next-generation biomanufacturing, the semiconductor-cyanobacterial hybrid systems are capable of enhancing the solar-to-chemical conversion efficiency. Herein, the native ethylene-producing capability of a filamentous cyanobacterium Nostoc sphaeroides is confirmed experimentally. The self-assembly characteristic of N.â sphaeroides is exploited to facilitate its interaction with InP nanomaterial, and the resulting biohybrid system gave rise to further elevated photosynthetic ethylene production. Based on chlorophyll fluorescence measurement and metabolic analysis, the InP nanomaterial-augmented photosystem I activity and enhanced ethylene production metabolism of biohybrid cells are confirmed, the mechanism underlying the material-cell energy transduction as well as nanomaterial-modulated photosynthetic light and dark reactions are established. This work not only demonstrates the potential application of semiconductor-N.â sphaeroides biohybrid system as a good platform for sustainable ethylene production but also provides an important reference for future studies to construct and optimize nano-cell biohybrid systems for efficient solar-driven valuable chemical production.
Assuntos
Etilenos , FotossínteseRESUMO
Broccoli sprouts have a strong ability to accumulate isothiocyanate and selenium. In this study, the isothiocyanate content increased significantly as a result of ZnSO4 stress. Particularly, based on the isothiocyanate content is not affected, the combined ZnSO4 and Na2SeO3 treatment alleviated the inhibition of ZnSO4 and induced selenium content. Gene transcription and protein expression analyses revealed the changes in isothiocyanate and selenium metabolite levels in broccoli sprouts. ZnSO4 combined with Na2SeO3 was proven to activate a series of isothiocyanate metabolite genes (UGT74B1, OX1, and ST5b) and selenium metabolite genes (BoSultr1;1, BoCOQ5-2, and BoHMT1). The relative abundance of the total 317 and 203 proteins, respectively, in 4-day-old broccoli sprouts varied, and the metabolic and biosynthetic pathways for secondary metabolites were significantly enriched in ZnSO4/control and ZnSO4 combined Na2SeO3/ZnSO4 comparisons. The findings demonstrated how ZnSO4 combined with Na2SeO3 treatment reduced stress inhibition and the accumulation of encouraged selenium and isothiocyanates during the growth of broccoli sprouts.
Assuntos
Brassica , Selênio , Selênio/metabolismo , Proteoma/metabolismo , Isotiocianatos/metabolismo , Enxofre , Brassica/metabolismo , Glucosinolatos/metabolismo , Sulfóxidos/metabolismoRESUMO
Soybeans have medicinal value and are an oil crop with medicinal and food properties. The present work investigated two aspects of isoflavone accumulation in soybean. First, germination conditions for exogenous-ethephon-mediated accumulation of isoflavone were optimised through response surface methodology. Second, various influences of ethephon on the growth of germinating soybeans and isoflavone metabolism were investigated. The findings of the research led to the conclusion that exogenous ethephon treatment effectively facilitated the enrichment of isoflavones in soybeans during germination. Optimal germination conditions were obtained through a response surface optimization test, which yielded the following criteria: a germination time of 4.2 d, an ethephon concentration of 102.6 µM, and a germination temperature of 30.2 °C. The maximum isoflavone content was 544.53 µg/sprout FW. Relative to the control, the addition of ethephon significantly inhibited sprout growth. Exogenous ethephon treatment led to the phenomenon that peroxidase, superoxide dismutase, and catalase activities and their gene expression increased significantly in germinating soybeans. Meanwhile, the expression of genes related to ethylene synthetase increase under the effect of ethephon promoting ethylene synthesis. Ethylene multiplied the total flavonoid content of soybean sprouts relying on the increase in activity and gene expression of crucial isoflavone biosynthesis-related enzymes (phenylalanine ammonia-lyase and 4-coumarate coenzyme A ligase) during germination.
Assuntos
Isoflavonas , Isoflavonas/farmacologia , Isoflavonas/metabolismo , Glycine max/metabolismo , Vias Biossintéticas , Etilenos/metabolismo , AceleraçãoRESUMO
Broccoli sprouts have high isothiocyanate and selenium accumulation capacity. This study used a combination of methods, including physiological and biochemical, gene transcription and proteomic, to investigate the isothiocyanate and endogenous selenium accumulation mechanisms in broccoli sprouts under exogenous sodium selenite treatment during germination. Compared with the control, the sprouts length of broccoli sprouts under exogenous selenium treatment was significantly lower, and the contents of total phenol and malondialdehyde in 6-day-old broccoli sprouts were substantially higher. The contents of isothiocyanate and sulforaphane in 4-day-old were increased by up-regulating the relative expression of genes of UGT74B1, OX-1, and ST5b. The relative expression of BoSultr1;1, BoSMT, BoHMT1, and BoCOQ5-2 genes regulating selenium metabolism was significantly up-regulated. In addition, 354 proteins in 4-day-old broccoli sprouts showed different relative abundance compared to the control under selenium treatment. These proteins were classified into 14 functional categories. It was discovered that metabolic pathways and biosynthetic pathways of secondary metabolites were significantly enriched. The above results showed that exogenous selenium was beneficial in inducing the accumulation of isothiocyanate and selenium during the growth of broccoli sprouts.
RESUMO
Organic-inorganic hybrid perovskite solar cells (PSCs) are among the most promising candidates for the next generation of photovoltaic devices because of the significant increase in their power conversion efficiency (PCE) from less than 10% to 25.7% in past decade. The metal-organic framework (MOF) materials owing to their unique properties, such as large specific surface area, abundant binding sites, adjustable nanostructures, and synergistic effects, are used as additives or functional layers to enhance the device performance and long-term stability of PSCs. This review focuses on the recent advancements in the applications of MOFs as/in different functional layers of PSCs. The photovoltaic performance, impact, and advantages of MOF materials integrated into the perovskite absorber, electron transport layer, hole transport layer, and interfacial layer are reviewed. In addition, the applicability of MOFs to mitigate leakage of Pb2+ from halide perovskites and corresponding devices is discussed. This review concludes with the perspectives on further research directions for employing MOFs in PSCs.
RESUMO
In this study, the effects of methyl jasmonate (MeJA) and sodium chloride (NaCl) treatments on the resveratrol biosynthesis and physiology of peanuts during germination were investigated. The results showed that MeJA (150 µM) and NaCl (150 mM) treatments significantly promoted resveratrol biosynthesis in germinated peanuts. MeJA and NaCl treatments promoted resveratrol accumulation by regulating the activities of phenylalanine ammonia lyase (PAL), cinnamic acid 4-hydroxylase (C4H) and 4-coumarate coenzyme A ligase (4CL) and their gene expression levels in cotyledons and non-cotyledons. In addition, both MeJA and NaCl treatments inhibited peanut sprout growth, as evidenced by shorter sprout length, increased malondialdehyde content, and accumulation of reactive oxygen species in cotyledons and non-cotyledons. Both treatments' germinated peanuts responded to the environmental stimuli by raising the activities of antioxidant enzymes and controlling the levels of their gene' expression. Meanwhile, MeJA and NaCl treatments promoted Ca2+ aggregation in the root tips. Therefore, it can be deduced that Ca2+ may help improve the plant's resistance to adversity. In conclusion, treatment with MeJA (150 µM) or NaCl (150 mM) during germination is an effective way to enrich the resveratrol content of peanuts. Germinated peanuts enhance adaptation to the external environment by promoting resveratrol biosynthesis and enhancing antioxidant systems.
Assuntos
Antioxidantes , Arachis , Resveratrol/farmacologia , Antioxidantes/metabolismo , Arachis/metabolismo , Cloreto de Sódio/farmacologia , Cloreto de Sódio/metabolismo , Acetatos/farmacologia , Ciclopentanos/farmacologia , Oxilipinas/farmacologiaRESUMO
The involvement of nitric oxide (NO) in exogenous melatonin (MT)-induced isoflavone accumulation and growth improvement in NaCl-stressed soybeans was investigated in this study. The results demonstrated that MT increased the activity of nitrate reductase (NR) and upregulated the relative expression of NR1, NR2, and nitric oxide synthase1, which subsequently led to an increase in NO content. MT and sodium nitroprusside (SNP, as an NO donor) markedly increased isoflavone content by enhancing the activities of cinnamic acid 4-hydroxylase (C4H) and phenylalanine ammonia lyase (PAL), and by upregulating gene expression of C4H, Isoflavone synthase, PAL, and Chalcone isomerase 1A, which are involved in isoflavone biosynthesis. Moreover, MT, as well as SNP, improved the growth and biomass of NaCl-treated soybeans by increasing the activities of superoxide dismutase, catalase, and peroxidase, and reducing the accumulation of H2O2 and O2â¢- in soybeans under NaCl stress. These MT-induced responses were entirely reversed by the supply of 4-carboxyphenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO, a specific scavenger of NO), which in turn considerably decreased endogenous NO content. These results suggest that NO acts as an important downstream signal molecule, mediating MT-induced isoflavone accumulation and growth improvement in NaCl-stressed soybeans.
Assuntos
Fabaceae , Isoflavonas , Melatonina , Glycine max , Isoflavonas/farmacologia , Cloreto de Sódio/farmacologia , Óxido Nítrico , Melatonina/farmacologia , Peróxido de Hidrogênio , Fenilalanina Amônia-LiaseRESUMO
Phenolic acid is a physiologically active substance that has a variety of effects on humans. Barley sprouts are often used as food ingredients to enrich phenolic acids and to further produce functional foods rich in phenolic acids. In this study, the mechanism of Ca2+ involvement in regulating phenolic acid biosynthesis and plant growth in barley by melatonin (MT) under NaCl stress was investigated. According to the studies, MT (25 µM) increased total calcium content, induced Ca2+ burst, and up-regulated the gene expression of calcium-regulated protein-dependent protein kinase and calcium-binding protein transcription-activating protease in NaCl-stressed (60 mM) barley. Exogenous MT and its combined CaCl2 (0.4 mM) significantly promoted phenolic acid biosynthesis by increasing the activity of C4H and PAL, and induced gene expression of PAL and F5H. The addition of exogenous CaCl2 and MT caused systemic tolerance in NaCl-stressed barley, as determined by a decrease in the fluorescence intensity of hydrogen peroxide and oxygen radical anions as well as an enhancement in the antioxidant enzyme, thus significantly increasing sprout length and fresh weight. In addition, combined use of MT with Ca2+ antagonists (lanthanum chloride or ethylene glycol tetraacetic acid), impaired all impacts as mentioned above. These findings imply that Ca2+ participated in MT-induced phenolic acid biosynthesis and growth improvement in NaCl-stressed barley.