Cell damage and apoptosis in the hepatopancreas of Eriocheir sinensis induced by cadmium.

Cadmium (Cd) is one of the most common pollutants in the environment and it is known to cause a range of tissue damages and apoptosis in invertebrates. In this study, we investigated the effect of Cd on the hepatopancreas of the crab Eriocheir sinensis, a commercially and ecologically important species of crustacean. The crabs were first exposed to water containing different concentrations of Cd2+ (0, 0.63, 1.26, 2.52, 5.04 and 10.07mg/L) for 6days. Typical morphological characteristics and physiological changes of apoptosis were then observed using various methods, including AO/EB double fluorescence staining, transmission electron microscopy and DNA fragmentation analysis. The results showed that Cd2+ induced cell damage and apoptosis in a concentration-dependent manner. Transmission electron microscopy revealed the presence of cellular swelling and necrosis with reduced number of microvilli on the cell surface and damages to individual organelles. The mitochondria became swollen and vacuolated. The rough endoplasmic reticulum (Rer) was expanded, with membrane rupture and many different sizes of vesicles, suggesting the destruction of protein-synthesizing structures in the hepatopancreatic cells. The activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidases (GPx) initially increased and subsequently decreased with increasing Cd2+ concentrations. This was accompanied by increases in malondialdehyde (MDA) and H2O2 contents, which led to membrane lipid peroxidation. Crabs exposed to Cd2+ also displayed significant increases in caspase-3, -8, and -9 activities compared to control crabs. Cadmium induced the production and accumulation of ROS in the hepatopancreas, which resulted in oxidative damage and abnormal metabolism. Taken together, the results indicated that Cd2+ could induce oxidative damage as well caspase-dependent apoptosis in E. sinensis hepatopancreas.

The fine structure of coelomocytes in the sipunculid Phascolosoma esculenta.

Ultrastructural characteristics of coelomocytes of the sipunculid Phascolosoma esculenta were studied by transmission electron microscopy. There are several cell types in the coelomic fluid, including three kinds of granulocytes, vesicular cells, germ cells, amoebocytes, phagocytes, and erythrocytes; there are also a new cell complex which is composed of podocytes and granular cells. And several other cell types (erythrocyte and different kinds of granulocytes) gathering together was discovered in the coelomic fluid of P. esculenta. Functional interpretations were provided for these cells using morphological evidence. The coelomocytes from different sipunculid genera and Annelida were compared. The structural diversity of coelomocytes provides both taxonomic characteristics for discriminative identification and phylogenetic markers in Phascolosoma and other sipunculid taxa.

KIFC1 participates in acrosomal biogenesis, with discussion of its importance for the perforatorium in the Chinese mitten crab Eriocheir sinensis.

Spermatogenesis is a complicated process during which spermatogonia undergo proliferation and divisions leading, after a series of dramatic changes, to the production of mature spermatozoa. Many molecular motors are involved in this process. KIFC1, a C-terminal kinesin motor, participates in acrosome biogenesis and nuclear shaping. We report here the expression profile of KIFC1 during spermatogenesis in the Chinese mitten crab, Eriocheir sinensis. KIFC1 mainly localizes around the nucleus but is also present within the nucleus of the spermatogonium and spermatocyte. At the early spermatid stage, KIFC1 begins to be distributed on the nuclear membrane at the region where the proacrosomal vesicle is located. By the late spermatid stage, KIFC1 is found on the acrosome. Immunocytochemical and ultrastructural analyses have shown that KIFC1 localizes on the perforatorium, which is composed of an apical cap and an acrosomal tubule. We demonstrate that, during spermatogenesis in E. sinensis, KIFC1 probably plays important roles in the biogenesis of the acrosome and in its maintenance. KIFC1 may also be essential for the eversion of the acrosome during fertilization.

Ultrastructure of spermatozoa of Bullacta exarata (philippi) and its significance on reproductive evolution and physio-ecological adaptation.

The morphology and ultrastructure of Bullacta exarata spermatozoa observed by light and transmission electron microscopy are presented in this paper. The spermatozoa is composed of head with a simple acrosomal complex and an elongated nucleus, and tail with a midpiece, principal piece and an end piece. The midpiece consists of a mitochondrial ring, and the principal piece is composed of axoneme and lateral fin. The structure of B. exarata spermatozoa differs significantly from that of other gastropods, especially in the lateral fin and the principal piece, which was described scarcely before. A comparison is made between B. exarata and other gastropods, and its significance on reproductive evolution and physio-ecological adaptation is preliminarily discussed.