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Plant Physiol Biochem ; 47(8): 724-31, 2009 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-19410476

RESUMO

Oligochitosan (OC) can regulate plant defense responses in many aspects, but the basic signal transduction pathway is still unclear. In this study, we used transgenic (TG) tobacco (Nicotiana Tabacum var. Samsun NN) as plant material whose oligochitosan induced protein kinase (OIPK) gene was inhibited by antisense transformation, to study the role of OIPK in tobacco defense reactions. The results showed that OIPK could increase tobacco resistance against tobacco mosaic virus (TMV), in that wild-type (WT) tobacco showed longer lesion appearance time, higher lesion inhibition ratio, smaller average final lesion diameter and lower average final lesion area percent to whole leaf area. It led us to analyze some pathogenesis related (PR) enzymes' activities and mRNA level, which played roles in tobacco resistance against TMV. We found that phenylalanine ammonia-lyase (PAL) and peroxidase (POD) activities were positively related to OIPK, but not polyphenol oxidase (PPO). It was also demonstrated that OIPK mRNA could be induced by OC, wound and TMV infection. In addition, OIPK could up-regulated three PR genes, PAL, chitinase (CHI) and beta-1, 3-glucanase (GLU) mRNA level to different extent. Taken together, these results implied that OIPK could function in tobacco resistance against both biotic and abiotic stress, possibly via various PR proteins.


Assuntos
Quitina/análogos & derivados , Regulação da Expressão Gênica de Plantas , Nicotiana/enzimologia , Doenças das Plantas/virologia , Proteínas de Plantas/genética , Proteínas Quinases/genética , Proteínas Serina-Treonina Quinases/genética , Vírus do Mosaico do Tabaco/patogenicidade , Catecol Oxidase/genética , Catecol Oxidase/metabolismo , Quitina/metabolismo , Quitinases/genética , Quitinases/metabolismo , Quitosana , Expressão Gênica , Glucana 1,3-beta-Glucosidase/genética , Glucana 1,3-beta-Glucosidase/metabolismo , Oligossacarídeos , Peroxidase/genética , Peroxidase/metabolismo , Fenilalanina Amônia-Liase/genética , Fenilalanina Amônia-Liase/metabolismo , Doenças das Plantas/genética , Folhas de Planta/efeitos dos fármacos , Folhas de Planta/enzimologia , Folhas de Planta/genética , Proteínas de Plantas/metabolismo , Plantas Geneticamente Modificadas , Proteínas Quinases/metabolismo , Proteínas Serina-Treonina Quinases/metabolismo , RNA Mensageiro/metabolismo , Transdução de Sinais , Nicotiana/efeitos dos fármacos , Nicotiana/genética , Regulação para Cima
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