RESUMO
Analysis of polymorphisms of genes encoding antioxidant enzymes SOD1 (G7958A), SOD2 (T58C), CAT (C-262T), and GSTP1 (Ile105Val) in 93 patients with post-traumatic gonarthrosis showed that GSTP1 Ile105Val polymorphism is often associated with heterozygous mutation in catalase gene CAT C-262T. In gonarthrosis, catalase activity in peripheral blood mononuclear cells in patients with CT genotype of the C-262T locus of CAT gene more than 2-fold surpassed that in CC genotype and more than 50% surpassed the normal. Changes in the balance of activity of antioxidant enzymes can affect viability of mononuclear cells.
Assuntos
Antioxidantes/metabolismo , Polimorfismo Genético/genética , Adulto , Catalase/genética , Feminino , Predisposição Genética para Doença , Glutationa S-Transferase pi/genética , Humanos , Traumatismos do Joelho/genética , Masculino , Pessoa de Meia-Idade , Superóxido Dismutase/genéticaRESUMO
Post-traumatic knee osteoarthritis patients (n=117) and healthy people (n=94) were investigated to determine the genotypic and allelic frequency distribution of polymorphic loci Т-31С, С+3953Т IL-1ß and G-308A TNF-α. The plasma and synovial fluid levels of proinflammatory cytokines IL-1ß and TNF-α were measured in 31 patients. The research demonstrated that the blood and synovial fluid levels of IL-1ß but not TNF-α were increased in patients with post-traumatic knee osteoarthritis. Also, the linkage between TNF-α level and polymorphic locus G-308A TNF-α was shown: the presence of allele -308Ð was associated with decreased TNF-α level. We demonstrated that polymorphic loci Т-31С, С+3953Т IL-1ß and G-308A TNF-α do not predict the higher risk of post-traumatic knee osteoarthritis development in Russian population of Rostov-on-Don region.
Assuntos
Osteoartrite do Joelho , Polimorfismo Genético , Citocinas , Humanos , Articulação do Joelho , Federação Russa , Líquido Sinovial , Fator de Necrose Tumoral alfaRESUMO
The features of regulation of free radical oxidation in the blood and the synovial fluid have been studied in patients with knee osteoarthritis. The data were obtained from 46 patients with primary knee osteoarthritis (II-III Kellgren-Lawrence grade, aged 63.26 ± 9.18) and 27 healthy controls. It was defined that the blood plasma was characterized by increased superoxide-eliminating activity and a tendency to the increasing of nitrite/nitrate concentration (nitrosative stress biomarkers). The erythrocytes were measured to have the increased concentration of malondialdehyde (secondary product of lipid peroxidation), activation of superoxide dismutase, gIutathione-S-transferase and reduced glutathione peroxidase activity and glutathione content. The peripheral blood mononuclear fraction of patients with osteoarthritis was characterized by significant activation of superoxide dismutase and glutathione-S- transferase, moderate catalase activation, non-significant glutathione peroxidase activity alterations and increased activity of xanthine oxidoreductase and myeloperoxidase. Finally redox-balance disturbance in the mononuclear cells leaded to oxidative stress development, which promoted lymphocytes apoptosis.